The ＇double T-DNA＇ binary vector p13HSR which harbored two independent T-DNAs, containing hygromycin phosphotransferase gene （hpf） in one T-DNA region and three target genes （hLF, SB401, RZ10） in another T-DNA r...The ＇double T-DNA＇ binary vector p13HSR which harbored two independent T-DNAs, containing hygromycin phosphotransferase gene （hpf） in one T-DNA region and three target genes （hLF, SB401, RZ10） in another T-DNA region, was used to generate selectable marker-free transgenic rice by Agrobacterium-mediated transformation. The regenerated plants with both the three target genes and the selectable marker gene hpt were selected for anther culture. RT-PCR analysis indicated that target genes were inserted in rice genomic DNA and successfully transcribed. It took only one year to obtain double haploid selectable marker-free transgenic plants containing the three target genes with co-transformation followed by anther culture technique, and the efficiency was 12.2%. It was also noted that one or two target genes derived from the binary vector were lost in some transgenic rice plants.展开更多
Antimicrobial peptide is a polypeptide with antimicrobial activity. Antimicrobial peptide genes Np3 and Np5 from Chinese shrimp （Fenneropenaeus Chinensis） were integrated into Oryza sativa L. subsp, japonica cv. Aic...Antimicrobial peptide is a polypeptide with antimicrobial activity. Antimicrobial peptide genes Np3 and Np5 from Chinese shrimp （Fenneropenaeus Chinensis） were integrated into Oryza sativa L. subsp, japonica cv. Aichi ashahi by Agrobacterium mediated transformation system. PCR analysis showed that the positive ratios of Np3 and Np5 were 36% and 45% in To generation, respectively. RT-PCR analysis showed that the antimicrobial peptide genes were expressed in T1 generation, and there was no obvious difference in agronomic traits between transgenic plants and non-transgenic plants. Four Np3 and Np5 transgenic lines in T1 generation were inoculated with Xanthomonas oryzae pv. oryzae strain CR4, and all the four transgenic lines had significantly enhanced resistance to bacterial blight caused by the strain CR4. The Np5 transgenic lines also showed higher resistance to bacterial blight caused by strains JS97-2, Zhe 173 and OS-225. It is suggested that transgenic lines with Np5 gene might possess broad spectrum resistance to rice bacterial blight.展开更多
Forest structure analysis is important for understanding the properties and development of a forest community,and its outcomes can be influenced by how trees are measured in sampled plots.Although there is a general c...Forest structure analysis is important for understanding the properties and development of a forest community,and its outcomes can be influenced by how trees are measured in sampled plots.Although there is a general consensus on the height at which tree diameter should be measured[1.3 m:diameter at breast height(DBH)],the minimum measureddiameter(MMD)often varies in different studies.In this study,we assumed that the outcomes of forest structure analysis can be influenced by MMD and,to this end,we applied g(r)function and stand spatial structural parameters(SSSPs)to investigate how different MMDs affect forest spatial structure analysis in two pine-oak mixed forests(30 and 57 years old)in southwest China and one old-growth oak forest(>120years old)from northwest China.Our results showed that 1)MMD was closely related to the distribution patterns of forest trees.Tree distribution patterns at each observational scale(r=0-20 m)tended tobecome random as the MMD increased.The older the community,the earlier this random distribution pattern appeared.2)As the MMD increased,neighboring trees became more regularly distributed around a reference tree.In most cases,however,nearest neighbors of a reference tree were randomly distributed.3)Tree species mingling decreased with increasing diameter,but it decreased slowly in older forests.4)No correlations can be found between individual tree size differentiation and MMD.We recommend that comparisons of spatial structures between communities would be more effective if using a unified MMD criterion.展开更多
Panicles of an indica rice line TM7-5 were subjected to radiation with 137^Cs gamma rays at 0 （control）, 5, 10, 15 and 20 Gy respectively, and then its anthers were cultured. There were slight differences among the ...Panicles of an indica rice line TM7-5 were subjected to radiation with 137^Cs gamma rays at 0 （control）, 5, 10, 15 and 20 Gy respectively, and then its anthers were cultured. There were slight differences among the treatments in peak emerging time of callus initiation, from 38 to 44 days after inoculation （DAI） as well as the frequency of callus initiation （2.3-3.5%）. About two thirds calli were induced before 44 DAI, and calli derived beyond 60 DAI lost the regeneration ability. Green plant regeneration frequency was significantly stimulated from two- to three-fold by irradiation of the 1370S gamma rays compared with the control, and the maximum was 22,81% （15 Gy）. The culture ability based on callus initiation and green plantlet regeneration was 0.19% for the control while it was over 0.45% for all the irradiated treatments, and the maximum was 0,59% for 15 Gy treatment. The advantages of panicle irradiation before anther culture and the potential application in rice anther culture, especially for recalcitrant indica rice, were discussed.展开更多
This paper analyses a poem Desert Places written by Robert Frost from the aspects of sound and meter. The analysis shows that this poem is richly loaded with metrical devices, flavoring the poetic frame with strong se...This paper analyses a poem Desert Places written by Robert Frost from the aspects of sound and meter. The analysis shows that this poem is richly loaded with metrical devices, flavoring the poetic frame with strong sense of music, which work to satisfy the thought of this poem.展开更多
文摘The ＇double T-DNA＇ binary vector p13HSR which harbored two independent T-DNAs, containing hygromycin phosphotransferase gene （hpf） in one T-DNA region and three target genes （hLF, SB401, RZ10） in another T-DNA region, was used to generate selectable marker-free transgenic rice by Agrobacterium-mediated transformation. The regenerated plants with both the three target genes and the selectable marker gene hpt were selected for anther culture. RT-PCR analysis indicated that target genes were inserted in rice genomic DNA and successfully transcribed. It took only one year to obtain double haploid selectable marker-free transgenic plants containing the three target genes with co-transformation followed by anther culture technique, and the efficiency was 12.2%. It was also noted that one or two target genes derived from the binary vector were lost in some transgenic rice plants.
基金supported by the Important National Science & Technology Specific Projects for Breeding New Transgenic Varieties in China (Grant No. 2008ZX08010-004)
文摘Antimicrobial peptide is a polypeptide with antimicrobial activity. Antimicrobial peptide genes Np3 and Np5 from Chinese shrimp （Fenneropenaeus Chinensis） were integrated into Oryza sativa L. subsp, japonica cv. Aichi ashahi by Agrobacterium mediated transformation system. PCR analysis showed that the positive ratios of Np3 and Np5 were 36% and 45% in To generation, respectively. RT-PCR analysis showed that the antimicrobial peptide genes were expressed in T1 generation, and there was no obvious difference in agronomic traits between transgenic plants and non-transgenic plants. Four Np3 and Np5 transgenic lines in T1 generation were inoculated with Xanthomonas oryzae pv. oryzae strain CR4, and all the four transgenic lines had significantly enhanced resistance to bacterial blight caused by the strain CR4. The Np5 transgenic lines also showed higher resistance to bacterial blight caused by strains JS97-2, Zhe 173 and OS-225. It is suggested that transgenic lines with Np5 gene might possess broad spectrum resistance to rice bacterial blight.
基金financially supported by the National Science Foundation of China (grant no. 31400542 31460196)+1 种基金Guangxi Natural Science Foundation (grant 2016GXNSFBA380233)Guangxi special fund project for innovation-driven development (AA 17204087-8)
文摘Forest structure analysis is important for understanding the properties and development of a forest community,and its outcomes can be influenced by how trees are measured in sampled plots.Although there is a general consensus on the height at which tree diameter should be measured[1.3 m:diameter at breast height(DBH)],the minimum measureddiameter(MMD)often varies in different studies.In this study,we assumed that the outcomes of forest structure analysis can be influenced by MMD and,to this end,we applied g(r)function and stand spatial structural parameters(SSSPs)to investigate how different MMDs affect forest spatial structure analysis in two pine-oak mixed forests(30 and 57 years old)in southwest China and one old-growth oak forest(>120years old)from northwest China.Our results showed that 1)MMD was closely related to the distribution patterns of forest trees.Tree distribution patterns at each observational scale(r=0-20 m)tended tobecome random as the MMD increased.The older the community,the earlier this random distribution pattern appeared.2)As the MMD increased,neighboring trees became more regularly distributed around a reference tree.In most cases,however,nearest neighbors of a reference tree were randomly distributed.3)Tree species mingling decreased with increasing diameter,but it decreased slowly in older forests.4)No correlations can be found between individual tree size differentiation and MMD.We recommend that comparisons of spatial structures between communities would be more effective if using a unified MMD criterion.
文摘Panicles of an indica rice line TM7-5 were subjected to radiation with 137^Cs gamma rays at 0 （control）, 5, 10, 15 and 20 Gy respectively, and then its anthers were cultured. There were slight differences among the treatments in peak emerging time of callus initiation, from 38 to 44 days after inoculation （DAI） as well as the frequency of callus initiation （2.3-3.5%）. About two thirds calli were induced before 44 DAI, and calli derived beyond 60 DAI lost the regeneration ability. Green plant regeneration frequency was significantly stimulated from two- to three-fold by irradiation of the 1370S gamma rays compared with the control, and the maximum was 22,81% （15 Gy）. The culture ability based on callus initiation and green plantlet regeneration was 0.19% for the control while it was over 0.45% for all the irradiated treatments, and the maximum was 0,59% for 15 Gy treatment. The advantages of panicle irradiation before anther culture and the potential application in rice anther culture, especially for recalcitrant indica rice, were discussed.
文摘This paper analyses a poem Desert Places written by Robert Frost from the aspects of sound and meter. The analysis shows that this poem is richly loaded with metrical devices, flavoring the poetic frame with strong sense of music, which work to satisfy the thought of this poem.
