Emerging evidence suggests that microbial dysbiosis plays vital roles in many human cancers.However,knowledge of whether the microbial community in thyroid tumor is related to tumorigenesis remains elusive.In this stu...Emerging evidence suggests that microbial dysbiosis plays vital roles in many human cancers.However,knowledge of whether the microbial community in thyroid tumor is related to tumorigenesis remains elusive.In this study,we aimed to explore the microbial community in thyroid tissues and its contribution to papillary thyroid cancer(PTC).In parallel,we performed microbial profiling and transcriptome sequencing in the tumor and adjacent normal tissues of a large cohort of 340 PTC and benign thyroid nodule(BTN)patients.Distinct microbial signatures were identified in PTC,BTN,and their adjacent nontumor tissues.Intra-thyroid tissue bacteria were verified by means of bacteria staining,fluorescence in situ hybridization,and immunoelectron microscopy.We found that 17 bacterial taxa were differentially abundant in PTC compared with BTN,which included enrichment in PTC of the pathobionts Rhodococcus,Neisseria,Streptococcus,Halomonas,and Devosia,and depletion of the beneficial bacteria Amycolatopsis.These differentially abundant bacteria could differentiate PTC tumor tissues(PTC-T)from BTN tissues(BTN-T)with an area under the curve(AUC)of 81.66%.Microbial network analysis showed increased correlation strengths among the bacterial taxa in PTC-T in comparison with BTN-T.Immunefunction-corresponding bacteria(i.e.,Erwinia,Bacillus,and Acinetobacter)were found to be enriched in PTC with Hashimoto’s thyroiditis.Moreover,our integrative analysis revealed that the PTC-enriched bacteria had a positive association with key PTC-oncogenic pathway-related genes,including BRAF,KRAS,IRAK4,CTNNB1,PIK3CA,MAP3K7,and EGFR.In conclusion,our results suggest that intratumor bacteria dysbiosis is associated with the thyroid tumorigenesis and oncogenic signaling pathways of PTC.展开更多
Objective:Limited effective interventions for advanced hepatocellular carcinoma(HCC)are available.This study aimed to investigate the potential clinical utility of apatinib,a highly selective inhibitor of the vascular...Objective:Limited effective interventions for advanced hepatocellular carcinoma(HCC)are available.This study aimed to investigate the potential clinical utility of apatinib,a highly selective inhibitor of the vascular endothelial growth factor receptor-2(VEGFR2)tyrosine kinase,as a radiosensitizer in the treatment of HCC.展开更多
Objective:Approximately 50%of hepatocellular carcinomas(HCCs)recur within 2 years after radiofrequency ablation(RFA).Cancer stem cells(CSCs)may play a critical role in the process of intrahepatic metastasis.Methods:We...Objective:Approximately 50%of hepatocellular carcinomas(HCCs)recur within 2 years after radiofrequency ablation(RFA).Cancer stem cells(CSCs)may play a critical role in the process of intrahepatic metastasis.Methods:We evaluated the migration and proliferation of HCC cell lines after sublethal heat treatment by performing transwell and cell counting assays.展开更多
Aims: To investigate the diagnostic value of fecal calprotectin (FC) determined by a new immunofluorescence assay-fluorescence enzyme immunoassay (FEIA) in patient with inflammatory bowel disease (IBD) or functional b...Aims: To investigate the diagnostic value of fecal calprotectin (FC) determined by a new immunofluorescence assay-fluorescence enzyme immunoassay (FEIA) in patient with inflammatory bowel disease (IBD) or functional bowel disease, compared with the typical ELISA kit. Methods: FC was determined simultaneously by FEIA and an ELISA kit in 26 patients with functional bowel disease and 77 patients with IBD. We compared the difference of FC levels between patients with IBD and patients with functional bowel disease. Receiver operating characteristics curve (ROC) was constructed to obtain the optimal cut-off value of FC for distinguishing IBD from functional bowel disease and the corresponding sensitivity and specificity. Results: The median FC levels of patients with IBD in clinical active stage or clinical remission stage was significantly higher than that of patients with functional bowel disease. The median FC levels of patients with IBD in clinical active stage, IBD in clinical remission stage and functional bowel disease were as follow: 699.91 (346.14 ~ 1647.54) μg/g;407.36 (121.81 ~ 878.48) μg/g;39.04 (12.09 ~ 81.04) μg/g when FC was measured by FEIA. The median FC levels were 716.99 (240.42 ~ 1232.53) μg/g;338.46 (53.08 ~ 692.82) μg/g;41.44 (11.77 ~ 73.19) μg/g among such above three groups of patients respectively, when FC was measured by ELISA kit. The diagnostic value of IBD with FC determined by FEIA (optimal cut-off = 131.79 μg/g) and ELISA kit (optimal cut-off = 121.85 μg/g) presented an area under the curve of 0.881 and 0.873, respectively. Conclusions: FC determined by FEIA was an accurate surrogate marker to distinguish IBD from functional bowel disease.展开更多
To the Editor:Even with the emergence of highly active antiretroviral therapy,human immunodeficiency virus type 1(HIV-1)remains a serious public health problem worldwide.The widespread use of antiretroviral therapy(AR...To the Editor:Even with the emergence of highly active antiretroviral therapy,human immunodeficiency virus type 1(HIV-1)remains a serious public health problem worldwide.The widespread use of antiretroviral therapy(ART)has,on the one hand,significantly improved life expectancy in patients infected with HIV-1;on the other hand,it has inevitably brought along with it a difficult problem—viral resistance.Viral resistance is a common phenomenon evolutionarily,particularly in patients with poor adherence and suboptimal drug levels.展开更多
We report on N,N-bidentate-chelation-assistedα-and β-olefinic C–H alkenylation of aryl alkenes in ethanol to afford aryl dienes/trienes with excellent regio-and stereo-selectivities.The reaction of 2-alkenyl benzyl...We report on N,N-bidentate-chelation-assistedα-and β-olefinic C–H alkenylation of aryl alkenes in ethanol to afford aryl dienes/trienes with excellent regio-and stereo-selectivities.The reaction of 2-alkenyl benzylamine and benzoic acid derived substrates proceeded through six-membered exo-cyclo-metallation and seven-membered endo-cyclometallation.The aerobic protocols feature wide functional-ity tolerance,high selectivities and yields,mild conditions and scalable preparation,and the directing group can be easily removed to afford Boc-protected amine by simple reduction.展开更多
Hepatocellular carcinoma(HCC)is the fourth leading cause of cancer-related deaths worldwide(1).Resident immune cell populations within the liver,which is considered the largest immune organ,increase the pathological c...Hepatocellular carcinoma(HCC)is the fourth leading cause of cancer-related deaths worldwide(1).Resident immune cell populations within the liver,which is considered the largest immune organ,increase the pathological complexity of HCC(2).Innate lymphoid cells(ILCs),recently identified leukocytes,are a family of innate immune cells with similar characters to T lymphocytes.ILCs largely exist in intestinal mucosal tissue and regulate intestinal inflammation,mucosal defense,and tissue repair in gut disease processes(3).Unlike T cells,ILCs lack antigen-specific receptors;therefore,they cannot directly mediate antigen-specific responses(4).However,they can be activated by cytokines and/or natural cytotoxic receptors(such as NKp44)(5).ILCs are a highly heterogeneous cell population with high plasticity.Based on the expression of transcription factors and the production of different cytokines,ILCs can be divided into ILC1,ILC2,and ILC3,which are counterparts of the three main helper T cell subsets(Th1,Th2,and Th17)(6).Although ILCs can be detected in various tumor types,their specific contribution to tumor immunity is yet to be thoroughly elucidated.展开更多
基金supported by the National Natural Science Foundation of China(81772850 and 82273300)。
文摘Emerging evidence suggests that microbial dysbiosis plays vital roles in many human cancers.However,knowledge of whether the microbial community in thyroid tumor is related to tumorigenesis remains elusive.In this study,we aimed to explore the microbial community in thyroid tissues and its contribution to papillary thyroid cancer(PTC).In parallel,we performed microbial profiling and transcriptome sequencing in the tumor and adjacent normal tissues of a large cohort of 340 PTC and benign thyroid nodule(BTN)patients.Distinct microbial signatures were identified in PTC,BTN,and their adjacent nontumor tissues.Intra-thyroid tissue bacteria were verified by means of bacteria staining,fluorescence in situ hybridization,and immunoelectron microscopy.We found that 17 bacterial taxa were differentially abundant in PTC compared with BTN,which included enrichment in PTC of the pathobionts Rhodococcus,Neisseria,Streptococcus,Halomonas,and Devosia,and depletion of the beneficial bacteria Amycolatopsis.These differentially abundant bacteria could differentiate PTC tumor tissues(PTC-T)from BTN tissues(BTN-T)with an area under the curve(AUC)of 81.66%.Microbial network analysis showed increased correlation strengths among the bacterial taxa in PTC-T in comparison with BTN-T.Immunefunction-corresponding bacteria(i.e.,Erwinia,Bacillus,and Acinetobacter)were found to be enriched in PTC with Hashimoto’s thyroiditis.Moreover,our integrative analysis revealed that the PTC-enriched bacteria had a positive association with key PTC-oncogenic pathway-related genes,including BRAF,KRAS,IRAK4,CTNNB1,PIK3CA,MAP3K7,and EGFR.In conclusion,our results suggest that intratumor bacteria dysbiosis is associated with the thyroid tumorigenesis and oncogenic signaling pathways of PTC.
文摘Objective:Limited effective interventions for advanced hepatocellular carcinoma(HCC)are available.This study aimed to investigate the potential clinical utility of apatinib,a highly selective inhibitor of the vascular endothelial growth factor receptor-2(VEGFR2)tyrosine kinase,as a radiosensitizer in the treatment of HCC.
文摘Objective:Approximately 50%of hepatocellular carcinomas(HCCs)recur within 2 years after radiofrequency ablation(RFA).Cancer stem cells(CSCs)may play a critical role in the process of intrahepatic metastasis.Methods:We evaluated the migration and proliferation of HCC cell lines after sublethal heat treatment by performing transwell and cell counting assays.
文摘Aims: To investigate the diagnostic value of fecal calprotectin (FC) determined by a new immunofluorescence assay-fluorescence enzyme immunoassay (FEIA) in patient with inflammatory bowel disease (IBD) or functional bowel disease, compared with the typical ELISA kit. Methods: FC was determined simultaneously by FEIA and an ELISA kit in 26 patients with functional bowel disease and 77 patients with IBD. We compared the difference of FC levels between patients with IBD and patients with functional bowel disease. Receiver operating characteristics curve (ROC) was constructed to obtain the optimal cut-off value of FC for distinguishing IBD from functional bowel disease and the corresponding sensitivity and specificity. Results: The median FC levels of patients with IBD in clinical active stage or clinical remission stage was significantly higher than that of patients with functional bowel disease. The median FC levels of patients with IBD in clinical active stage, IBD in clinical remission stage and functional bowel disease were as follow: 699.91 (346.14 ~ 1647.54) μg/g;407.36 (121.81 ~ 878.48) μg/g;39.04 (12.09 ~ 81.04) μg/g when FC was measured by FEIA. The median FC levels were 716.99 (240.42 ~ 1232.53) μg/g;338.46 (53.08 ~ 692.82) μg/g;41.44 (11.77 ~ 73.19) μg/g among such above three groups of patients respectively, when FC was measured by ELISA kit. The diagnostic value of IBD with FC determined by FEIA (optimal cut-off = 131.79 μg/g) and ELISA kit (optimal cut-off = 121.85 μg/g) presented an area under the curve of 0.881 and 0.873, respectively. Conclusions: FC determined by FEIA was an accurate surrogate marker to distinguish IBD from functional bowel disease.
基金Co-Sponsored Construction Project of Provincial Medicine and Technique of Henan Province(No. LHGJ20191103)。
文摘To the Editor:Even with the emergence of highly active antiretroviral therapy,human immunodeficiency virus type 1(HIV-1)remains a serious public health problem worldwide.The widespread use of antiretroviral therapy(ART)has,on the one hand,significantly improved life expectancy in patients infected with HIV-1;on the other hand,it has inevitably brought along with it a difficult problem—viral resistance.Viral resistance is a common phenomenon evolutionarily,particularly in patients with poor adherence and suboptimal drug levels.
基金We gratefully acknowledge the National Natural Science Foundation of China(NSFC)(21672048 and 81570989)Natural Science Foundation of Zhejiang Province(ZJNSF)(LY19B020006)+1 种基金Major Project of Hangzhou Health Science and Technology Plan(Z20200046)Key Subject of Stomatology in Hangzhou for financial support.
文摘We report on N,N-bidentate-chelation-assistedα-and β-olefinic C–H alkenylation of aryl alkenes in ethanol to afford aryl dienes/trienes with excellent regio-and stereo-selectivities.The reaction of 2-alkenyl benzylamine and benzoic acid derived substrates proceeded through six-membered exo-cyclo-metallation and seven-membered endo-cyclometallation.The aerobic protocols feature wide functional-ity tolerance,high selectivities and yields,mild conditions and scalable preparation,and the directing group can be easily removed to afford Boc-protected amine by simple reduction.
文摘Hepatocellular carcinoma(HCC)is the fourth leading cause of cancer-related deaths worldwide(1).Resident immune cell populations within the liver,which is considered the largest immune organ,increase the pathological complexity of HCC(2).Innate lymphoid cells(ILCs),recently identified leukocytes,are a family of innate immune cells with similar characters to T lymphocytes.ILCs largely exist in intestinal mucosal tissue and regulate intestinal inflammation,mucosal defense,and tissue repair in gut disease processes(3).Unlike T cells,ILCs lack antigen-specific receptors;therefore,they cannot directly mediate antigen-specific responses(4).However,they can be activated by cytokines and/or natural cytotoxic receptors(such as NKp44)(5).ILCs are a highly heterogeneous cell population with high plasticity.Based on the expression of transcription factors and the production of different cytokines,ILCs can be divided into ILC1,ILC2,and ILC3,which are counterparts of the three main helper T cell subsets(Th1,Th2,and Th17)(6).Although ILCs can be detected in various tumor types,their specific contribution to tumor immunity is yet to be thoroughly elucidated.
