A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method.Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of the...A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method.Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of the hand,and/or by injecting mannitol into the caudal vein twice daily.Brain tissue was collected at 24,48 and 72 hours after modeling,and blood was collected through the retinal vein before Evans blue was injected,approximately 1 hour prior to harvesting of brain tissue.Results showed that Evans blue leakage into brain tissue and serum nitric oxide synthase activity were significantly increased in model rats.Treatment with blood-letting punctures at twelve Jing-Well points of the hand and/or injection of mannitol into the caudal vein reduced the amount of Evans blue leakage into the brain tissue and serum nitric oxide synthase activity to varying degrees.There was no significant difference between single treatment and combined treatment.Experimental findings indicate that blood-letting punctures at twelve Jing-Well points of the hand can decrease blood-brain barrier permeability and serum nitric oxide synthase activity in rats following middle cerebral artery occlusion,and its effect is similar to that of mannitol injection alone and Jing-Well points plus mannitol injection.展开更多
A method for constructing core-germplasm of Rosa rugosa in China based on molecular marker data was probed and the optimum core germplasm was established. Studies were initiated to analyze the genetic diversity of 120...A method for constructing core-germplasm of Rosa rugosa in China based on molecular marker data was probed and the optimum core germplasm was established. Studies were initiated to analyze the genetic diversity of 120 rugged roses from 6 different Chinese source populations based on CDDP marker, and a preliminary construction of the core collection was established using stepwise UPGMA clustering sampling method. The 26 core collection resources of R. rugosa collections in China have 20% germplasm samples of initial collection, the retention ratio of polymorphic loci, effective number of alleles (Ne), Nei’s genetic diversity (H) and Shannon information index (I) were respectively 97.52%, 104.16%, 108.38% and 106.18%. The results of t-test showed that no significant difference was found in genetic diversity indexes between the core collection and the original collection. These results also demonstrated that the core collection could stand for original collection excellently. The results show that CDDP molecular technology can be successfully applied to the construction of core germplasm resources of rugged roses.展开更多
Mechanical force is crucial in the whole process of embryonic development.However,the role of trophoblast mechanics during embryo implantation has rarely been studied.In this study,we constructed a model to explore th...Mechanical force is crucial in the whole process of embryonic development.However,the role of trophoblast mechanics during embryo implantation has rarely been studied.In this study,we constructed a model to explore the effect of stiffness changes in mouse trophoblast stem cells(mTSCs)on implantation:microcarrier was prepared by sodium alginate using a droplet microfluidics system,and mTSCs were attached to the microcarrier surface with laminin modifications,called T(micro).Compared with the spheroid,formed by the self-assembly of mTSCs(T(sph)),we could regulate the stiffness of the microcarrier,making the Young’s modulus of mTSCs(367.70±79.81 Pa)similar to that of the blastocyst trophoblast ectoderm(432.49±151.90 Pa).Moreover,T(micro)contributes to improve the adhesion rate,expansion area and invasion depth of mTSCs.Further,T(micro)was highly expressed in tissue migration-related genes due to the activation of the Rho-associated coiled-coil containing protein kinase(ROCK)pathway at relatively similar modulus of trophoblast.Overall,our study explores the embryo implantation process with a new perspective,and provides theoretical support for understanding the effect of mechanics on embryo implantation.展开更多
Dear Editor,The placenta connecting the fetus to the matermal uterus provides material exchange and an immune-tolerant environment for the embryo in all eutherian mammals(Shao et al.,2022).The representative mouse pla...Dear Editor,The placenta connecting the fetus to the matermal uterus provides material exchange and an immune-tolerant environment for the embryo in all eutherian mammals(Shao et al.,2022).The representative mouse placenta displays a multilayered structure with distinct characteristics and functions,including the matermal decidua,junctional zone,and labyrinth layer(Marsh and Blelloch,2020).The decidua,which is thought to be derived from the matermal endometrium(and further undergoes decidualization),provides an anchor for fetal trophoblast invasion.The junctional zone predominantly contains spongiotrophoblasts(SpT),glycogen trophoblasts(GlyT),and trophoblast giant cells(TGCs).The labyrinth is the innermost two-layer structure,which mainly consists of syncytiotrophoblast cells(SynTI and SynTII),sinusoidal TGCs(S-TGCs),and fetal endothelial cells(Simmons and Cross,2005).展开更多
Effective methods for visualizing neurovascular morphology are essential for understanding the normal spinal cord and the morphological alterations associated with diseases.However,ideal techniques for simultaneously ...Effective methods for visualizing neurovascular morphology are essential for understanding the normal spinal cord and the morphological alterations associated with diseases.However,ideal techniques for simultaneously imaging neurovascular structure in a broad region of a specimen are still lacking.In this study,we combined Golgi staining with angiography and synchrotron radiation micro-computed tomography(SRμCT)to visualize the 3D neurovascular network in the mouse spinal cord.Using our method,the 3D neurons,nerve fibers,and vasculature in a broad region could be visualized in the same image at cellular resolution without destructive sectioning.Besides,we found that the 3D morphology of neurons,nerve fiber tracts,and vasculature visualized by SRjiCT were highly consistent with that visualized using the histological method.Moreover,the 3D neurovascular structure could be quantitatively evaluated by the combined methodology.The method shown here will be useful in fundamental neuroscience studies.展开更多
Following fertilization in mammals,the zygote initiates the developmental program,which has a transient capacity to generate cell types of both embryonic and extraembryonic lineages,which is defined as totipotency (Co...Following fertilization in mammals,the zygote initiates the developmental program,which has a transient capacity to generate cell types of both embryonic and extraembryonic lineages,which is defined as totipotency (Condic,2014),In mice, only zygotes and blastomeres of 2-cell stage embryos are considered totipotent,since they have the ability to develop into a full organism without the requirement of carrier cells (Tarkowski, 1959;Ishiuchi and Torres-Padilla,2013).However,the blastomeres of the 4-cell or later stage embryos quickly lose this ability (Rossant,1976).展开更多
In the field of developmental biology and regenerative medicine,mammalian interspecific chimeras have been proved very useful for investigating early embryonic development and the immune system establishment,and exten...In the field of developmental biology and regenerative medicine,mammalian interspecific chimeras have been proved very useful for investigating early embryonic development and the immune system establishment,and extended to a promising potential for human organ generation(Rossant et al.,1982).In the展开更多
基金sponsored by the Open Research Fund of Zhejiang First-foremost Key Subject-Acupuncture & Moxibustion,No. ZTK2010A07
文摘A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method.Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of the hand,and/or by injecting mannitol into the caudal vein twice daily.Brain tissue was collected at 24,48 and 72 hours after modeling,and blood was collected through the retinal vein before Evans blue was injected,approximately 1 hour prior to harvesting of brain tissue.Results showed that Evans blue leakage into brain tissue and serum nitric oxide synthase activity were significantly increased in model rats.Treatment with blood-letting punctures at twelve Jing-Well points of the hand and/or injection of mannitol into the caudal vein reduced the amount of Evans blue leakage into the brain tissue and serum nitric oxide synthase activity to varying degrees.There was no significant difference between single treatment and combined treatment.Experimental findings indicate that blood-letting punctures at twelve Jing-Well points of the hand can decrease blood-brain barrier permeability and serum nitric oxide synthase activity in rats following middle cerebral artery occlusion,and its effect is similar to that of mannitol injection alone and Jing-Well points plus mannitol injection.
文摘A method for constructing core-germplasm of Rosa rugosa in China based on molecular marker data was probed and the optimum core germplasm was established. Studies were initiated to analyze the genetic diversity of 120 rugged roses from 6 different Chinese source populations based on CDDP marker, and a preliminary construction of the core collection was established using stepwise UPGMA clustering sampling method. The 26 core collection resources of R. rugosa collections in China have 20% germplasm samples of initial collection, the retention ratio of polymorphic loci, effective number of alleles (Ne), Nei’s genetic diversity (H) and Shannon information index (I) were respectively 97.52%, 104.16%, 108.38% and 106.18%. The results of t-test showed that no significant difference was found in genetic diversity indexes between the core collection and the original collection. These results also demonstrated that the core collection could stand for original collection excellently. The results show that CDDP molecular technology can be successfully applied to the construction of core germplasm resources of rugged roses.
基金supported by National Natural Science Foundation of China(T2222029 and U21A20396)Strategic Priority Research Program of Chinese Academy of Sciences(XDA16020802)+1 种基金CAS Project for Young Scientists in Basic Research(YSBR-012)CAS Engineering Laboratory for Intelligent Organ Manufacturing(KFJ-PTXM-039).
文摘Mechanical force is crucial in the whole process of embryonic development.However,the role of trophoblast mechanics during embryo implantation has rarely been studied.In this study,we constructed a model to explore the effect of stiffness changes in mouse trophoblast stem cells(mTSCs)on implantation:microcarrier was prepared by sodium alginate using a droplet microfluidics system,and mTSCs were attached to the microcarrier surface with laminin modifications,called T(micro).Compared with the spheroid,formed by the self-assembly of mTSCs(T(sph)),we could regulate the stiffness of the microcarrier,making the Young’s modulus of mTSCs(367.70±79.81 Pa)similar to that of the blastocyst trophoblast ectoderm(432.49±151.90 Pa).Moreover,T(micro)contributes to improve the adhesion rate,expansion area and invasion depth of mTSCs.Further,T(micro)was highly expressed in tissue migration-related genes due to the activation of the Rho-associated coiled-coil containing protein kinase(ROCK)pathway at relatively similar modulus of trophoblast.Overall,our study explores the embryo implantation process with a new perspective,and provides theoretical support for understanding the effect of mechanics on embryo implantation.
基金supported by the National Key Research and Development Program of China(2018YFE0201100 to Lw.,2019YFA0110901 to G.F,2022YFA1104101 to GF,2022YFA1104300 to CL.)the Youth Innovation Promotion Association,CAS(2019084 to GF),informatization Plan of Chinese Academy of Sciences(CAS-Wx20215F-0101 to GF)the National Natural Science Foundation of China(31972895 toLw).
文摘Dear Editor,The placenta connecting the fetus to the matermal uterus provides material exchange and an immune-tolerant environment for the embryo in all eutherian mammals(Shao et al.,2022).The representative mouse placenta displays a multilayered structure with distinct characteristics and functions,including the matermal decidua,junctional zone,and labyrinth layer(Marsh and Blelloch,2020).The decidua,which is thought to be derived from the matermal endometrium(and further undergoes decidualization),provides an anchor for fetal trophoblast invasion.The junctional zone predominantly contains spongiotrophoblasts(SpT),glycogen trophoblasts(GlyT),and trophoblast giant cells(TGCs).The labyrinth is the innermost two-layer structure,which mainly consists of syncytiotrophoblast cells(SynTI and SynTII),sinusoidal TGCs(S-TGCs),and fetal endothelial cells(Simmons and Cross,2005).
基金by the National Natural Science Foundation of China(82030071,81874004,and 81672174)the Key R&D Program of the Hunan Provincial Science&Technology Department(2017SK2061)+1 种基金Hunan Provincial Department of Finance[(2018)2]by the Fundamental Research Funds for the Central Universities of Central South University(2018zzts254).
文摘Effective methods for visualizing neurovascular morphology are essential for understanding the normal spinal cord and the morphological alterations associated with diseases.However,ideal techniques for simultaneously imaging neurovascular structure in a broad region of a specimen are still lacking.In this study,we combined Golgi staining with angiography and synchrotron radiation micro-computed tomography(SRμCT)to visualize the 3D neurovascular network in the mouse spinal cord.Using our method,the 3D neurons,nerve fibers,and vasculature in a broad region could be visualized in the same image at cellular resolution without destructive sectioning.Besides,we found that the 3D morphology of neurons,nerve fiber tracts,and vasculature visualized by SRjiCT were highly consistent with that visualized using the histological method.Moreover,the 3D neurovascular structure could be quantitatively evaluated by the combined methodology.The method shown here will be useful in fundamental neuroscience studies.
基金supported by the National Natural Science Foundation of China (Nos. 31621004 and 81571356)the Key Research Projects of the Frontier Science of the Chinese Academy of Sciences (QYZDY-SSWSMC002)+1 种基金the Key Deployment Projects of the Chinese Academy of Sciences (ZDRW-ZS-2017-5)the Youth Innovation Promotion Association CAS (No. 2017113)
文摘Following fertilization in mammals,the zygote initiates the developmental program,which has a transient capacity to generate cell types of both embryonic and extraembryonic lineages,which is defined as totipotency (Condic,2014),In mice, only zygotes and blastomeres of 2-cell stage embryos are considered totipotent,since they have the ability to develop into a full organism without the requirement of carrier cells (Tarkowski, 1959;Ishiuchi and Torres-Padilla,2013).However,the blastomeres of the 4-cell or later stage embryos quickly lose this ability (Rossant,1976).
基金supported by the grants from the National Natural Science Foundation of China (Nos.31621004,31501188 and 31422038)the Key Research Projects of the Frontier Science of the Chinese Academy of Sciences (QYZDY-SSW-SMC002)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16000000)
文摘In the field of developmental biology and regenerative medicine,mammalian interspecific chimeras have been proved very useful for investigating early embryonic development and the immune system establishment,and extended to a promising potential for human organ generation(Rossant et al.,1982).In the
