The aim of this study is to demonstrate the influence of Spirulina platensis on the growth of Bifidobacteium breve in the infantile milk. The authors used a stump of Bifidobacterium breve isolated from the new saddles...The aim of this study is to demonstrate the influence of Spirulina platensis on the growth of Bifidobacteium breve in the infantile milk. The authors used a stump of Bifidobacterium breve isolated from the new saddles which was born after identification and characterization. The follow-up of the kinetics of growth of B. breve in the milk enriched by various concentrations of Spirulina (0, 1, 5 and 10 mg/mL) was realized, and the authors estimated the antimicrobial effect of the cultures realised on three pathogenic bacteria: a bacteria isolated from saddles "Salmonella ssp.", two reference stumps "Staphylococcus aureus ATCC25923" and "Escherichia coli ATCC25922". The growth rate was 0.11 hl for the culture of B. breve associated with Spirulina (10 mg/mL), 0.09 hl for the culture of B. breve enriched with Spirulina (5 mg/mL) and 0.08 hl for the culture of B. breve enriched with Spirulina (1 mg/mL). In the case of the pure culture of B. breve, the growth rate was 0.07 h1. The authors observed inhibitory effects of B. breve in the various cultures towards the considered pathogenic bacteria. The optimal antimicrobial activity was observed in the preculture bifid with the algae culture (10 mg/mL) towards Salmonella ssp. (Zi = 25 mm), S. aureus ATCC25923 (Zi = 26 mm) and E. coli ATCC25922 (Zi = 28 mm).展开更多
文摘The aim of this study is to demonstrate the influence of Spirulina platensis on the growth of Bifidobacteium breve in the infantile milk. The authors used a stump of Bifidobacterium breve isolated from the new saddles which was born after identification and characterization. The follow-up of the kinetics of growth of B. breve in the milk enriched by various concentrations of Spirulina (0, 1, 5 and 10 mg/mL) was realized, and the authors estimated the antimicrobial effect of the cultures realised on three pathogenic bacteria: a bacteria isolated from saddles "Salmonella ssp.", two reference stumps "Staphylococcus aureus ATCC25923" and "Escherichia coli ATCC25922". The growth rate was 0.11 hl for the culture of B. breve associated with Spirulina (10 mg/mL), 0.09 hl for the culture of B. breve enriched with Spirulina (5 mg/mL) and 0.08 hl for the culture of B. breve enriched with Spirulina (1 mg/mL). In the case of the pure culture of B. breve, the growth rate was 0.07 h1. The authors observed inhibitory effects of B. breve in the various cultures towards the considered pathogenic bacteria. The optimal antimicrobial activity was observed in the preculture bifid with the algae culture (10 mg/mL) towards Salmonella ssp. (Zi = 25 mm), S. aureus ATCC25923 (Zi = 26 mm) and E. coli ATCC25922 (Zi = 28 mm).