Tumor cell proliferation, infiltration, migration, and neovascularization are known causes of treatment resistance in glioblastoma multiforme(GBM). The purpose of this study was to determine the effect of radiation on...Tumor cell proliferation, infiltration, migration, and neovascularization are known causes of treatment resistance in glioblastoma multiforme(GBM). The purpose of this study was to determine the effect of radiation on the growth characteristics of primary human GBM developed in a nude rat. Primary GBM cells grown from explanted GBM tissues were implanted orthotopically in nude rats. Tumor growth was confirmed by magnetic resonance imaging on day 77(baseline) after implantation. The rats underwent irradiation to a dose of 50 Gy delivered subcuratively on day 84 postimplantation(n = 8), or underwent no radiation(n = 8). Brain tissues were obtained on day 112(nonirradiated) or day 133(irradiated). Immunohistochemistry was performed to determine tumor cell proliferation(Ki-67) and to assess the expression of infiltration marker(matrix metalloproteinase-2, MMP-2) and cell migration marker(CD44). Tumor neovascularization was assessed by microvessel density using von-Willebrand factor(vWF) staining. Magnetic resonance imaging showed well-developed, infiltrative tumors in 11 weeks postimplantation. The proportion of Ki-67-positive cells in tumors undergoing radiation was(71 ± 15)% compared with(25 ± 12)% in the nonirradiated group(P = 0.02). The number of MMP-2-positive areas and proportion of CD44-positive cells were also high in tumors receiving radiation, indicating great invasion and infiltration. Microvessel density analysis did not show a significant difference between nonirradiated and irradiated tumors. Taken together, we found that subcurative radiation significantly increased proliferation, invasion, and migration of primary GBM. Our study provides insights into possible mechanisms of treatment resistance following radiation therapy for GBM.展开更多
We analyze the spin coincidence experiment considered by Bell in the derivation of Bells theorem. We solve the equation of motion for the spin system with a spin Hamiltonian, Hz, where the magnetic field is only in th...We analyze the spin coincidence experiment considered by Bell in the derivation of Bells theorem. We solve the equation of motion for the spin system with a spin Hamiltonian, Hz, where the magnetic field is only in the z-direction. For the specific case of the coincidence experiment where the two magnets have the same orientation the Hamiltonian Hz commutes with the total spin Iz, which thus emerges as a constant of the motion. Bells argument is then that an observation of spin up at one magnet A necessarily implies spin down at the other B. For an isolated spin system A-B with classical translational degrees of freedom and an initial spin singlet state there is no force on the spin particles A and B. The spins are fully entangled but none of the spin particles A or B are deflected by the Stern-Gerlach magnets. This result is not compatible with Bells assumption that spin 1/2 particles are deected in a Stern-Gerlach device. Assuming a more realistic Hamiltonian Hz + Hx including a gradient in x direction the total Iz is not conserved and fully entanglement is not expected in this case. The conclusion is that Bells theorem is not applicable to spin coincidence measurement originally discussed by Bell.展开更多
Aims: To investigate whether intravesical lower pH induces a micturition threshold reflex change of anaesthetized rats. Methods: Thirteen female rats, anaesthetized by a-chloralose and paralyzed by pancurone bromide w...Aims: To investigate whether intravesical lower pH induces a micturition threshold reflex change of anaesthetized rats. Methods: Thirteen female rats, anaesthetized by a-chloralose and paralyzed by pancurone bromide were used for the experiments. The micturition threshold volume was determined by repeated cystometry. Simultaneously bladder afferent activities in four and efferent activities in seven rats were recorded from the exposed pelvic nerve to the bladder. Results: Cystometry with acidic saline at pH 3 and 4 induced a significant decrease in micturition threshold volume. The mean value of these groups of test showed that bladder compliance, micturition threshold pressure and maximal micturition contraction pressure were decreased significantly comparing with the controls. There were no changes in all other parameters recorded from afferent and efferent, e.g. afferent threshold volume, afferent or efferent activity at the micturition threshold and their peak activity at the micturition contraction. Conclusions: Acidic solution provoked sensitivity but reduced the force of the contractility to the urinary bladder. The effect was presented without a detectable change in the firing properties of bladder Ad mechanoreceptor afferents. It is proposed that proton sensitive bladder receptors with unmyelinated afferent were stimulated by the acidic solution and that the micturition reflex was facilitated by afferent inflow from such receptors. The findings were clinically important in choosing the medium for urodynamic examination.展开更多
The noncontact atomic force microscope(NCAFM)using frequency modulation(FM)technique can resolve the atomic features of the various surfaces.Recently,we found that the NC-AFM has a capability to identify or recogn... The noncontact atomic force microscope(NCAFM)using frequency modulation(FM)technique can resolve the atomic features of the various surfaces.Recently,we found that the NC-AFM has a capability to identify or recognize atom species on a sample surface,if we can control an atomic species at the tip apex.That is,we succeeded in identification of Si and Ge atoms by imaging the Si/Ge intermixing Si(111)Surface using Si tip,where both of Si and Ge atoms are group IV elements[1].In the present experiments,we further investigate the capability of NC-AFM to identify the atom species under the surface.……展开更多
b Département de Physique, Ecole Polythechnique Fédérale de Lausanne, EPFL, Lausanne, Switzerland c Department of Chemistry, Tsinghua University, Beijing 100084, China An excellent hole-transpor...b Département de Physique, Ecole Polythechnique Fédérale de Lausanne, EPFL, Lausanne, Switzerland c Department of Chemistry, Tsinghua University, Beijing 100084, China An excellent hole-transport material, 1,3-diphenyl-5-(9-phenanthryl)-2-pyrazoline (DPPhP) for OLEDs was studied. This compound not only offers high glass transition temperature (T g=96 ℃), good film forming ability, and high HOMO energy level, but also displays excellent hole-transport property. The electroluminescent device with a simple structure of ITO/DPPhP (60 nm)/AlQ (60 nm)/LiF (0.8 nm)/Al shows an external quantum efficiency as high as 1.6%.展开更多
To investigate the nature of the Ψ（3770） resonance and to measure the cross section for e^＋e^-→DD, a cross-section scan data sample, distributed among 41 center-of-mass energy points from 3.73 to 3.89 GeV, was ta...To investigate the nature of the Ψ（3770） resonance and to measure the cross section for e^＋e^-→DD, a cross-section scan data sample, distributed among 41 center-of-mass energy points from 3.73 to 3.89 GeV, was taken with the BESIII detector operated at the BEPCII collider in the year 2010. By analyzing the large angle Bhabha scattering events, we measure the integrated luminosity of the data sample at each center-of-mass energy point. The total integrated luminosity of the data sample is 76.16±0.04±0.61 pb^-1, where the first uncertainty is statistical and the second systematic.展开更多
Background Recently congenital infection with Schistosoma japonicum (S. japonicum) has been domonstrated in pigs, rabbits, mice and dogs. We explored the rabbit as an animal model for the congenital infection of schi...Background Recently congenital infection with Schistosoma japonicum (S. japonicum) has been domonstrated in pigs, rabbits, mice and dogs. We explored the rabbit as an animal model for the congenital infection of schistosomiasis japonica and assessed the effect of a congenital S. japonicum infection on the resistance of rabbit kittens to a postnatal challenge infection.Methods Sixteen pregnant New Zealand white rabbits were infected with a single dose of S. japonicum cercariae. The exposed animals were divided into three groups according to the gestation age at the time of infection. Diagnosis of prenatally acquired S. japonicum infection in the rabbit kittens was primarily based on serological tests in combination with parasitological and histopathological findings. Congenitally infected kittens were challenged percutaneously with 100 S. japonicum cercariae to assess the effect of a congenital S. japonicum infection on kitten resistance to a postnatal challenge infection.Results The overall prevalence of congenital infection in offspring of infected mothers was 20% (12/60). The congenital infection rate in group L (late gestation) was much higher than in group E (early gestation) and group M (mid-gestation) (P<0.05). After a postnatal challenge infection, prenatally infected kittens had a 54.66% worm reduction rate, 41.45% egg reduction rate, and 51.76% granuloma size reduction rate compared to nave kittens.Conclusions This study demonstrates the possibility of congenital infection of S. japonicum in rabbits and the resistance of congenitally infected kittens to a postnatal challenge infection. These results have important implications not only for epidemiological investigations, but also in designing government control programs for schistosomiasis.展开更多
We report new measurements of the cross sections for the production of DD final states at theψ（3770）resonance.Our data sample consists of an integrated luminosity of 2.93 fb^（-1）of e~＋e^-annihilation data produc...We report new measurements of the cross sections for the production of DD final states at theψ（3770）resonance.Our data sample consists of an integrated luminosity of 2.93 fb^（-1）of e~＋e^-annihilation data produced by the BEPCⅡ collider and collected and analyzed with the BESⅢ detector.We exclusively reconstruct three D^0and six D~＋hadronic decay modes and use the ratio of the yield of fully reconstructed DD events（“double tags”）to the yield of all reconstructed D or D mesons（“single tags”）to determine the number of D^0 D^0and D~＋D^-events,benefiting from the cancellation of many systematic uncertainties.Combining these yields with an independent determination of the integrated luminosity of the data sample,we find the cross sections to beσ（e~＋e^-→D^0D^0）=（3.615±0.010±0.038）nb andσ（e~＋e^-→D~＋D^-）=（2.830±0.011±0.026）nb,where the uncertainties are statistical and systematic,respectively.展开更多
基金supported by grants from the National Institutes of Health (NIH) [No. K25CA129173 (MMA), R01CA122031 (ASA), and 1R01CA160216 (ASA)]
文摘Tumor cell proliferation, infiltration, migration, and neovascularization are known causes of treatment resistance in glioblastoma multiforme(GBM). The purpose of this study was to determine the effect of radiation on the growth characteristics of primary human GBM developed in a nude rat. Primary GBM cells grown from explanted GBM tissues were implanted orthotopically in nude rats. Tumor growth was confirmed by magnetic resonance imaging on day 77(baseline) after implantation. The rats underwent irradiation to a dose of 50 Gy delivered subcuratively on day 84 postimplantation(n = 8), or underwent no radiation(n = 8). Brain tissues were obtained on day 112(nonirradiated) or day 133(irradiated). Immunohistochemistry was performed to determine tumor cell proliferation(Ki-67) and to assess the expression of infiltration marker(matrix metalloproteinase-2, MMP-2) and cell migration marker(CD44). Tumor neovascularization was assessed by microvessel density using von-Willebrand factor(vWF) staining. Magnetic resonance imaging showed well-developed, infiltrative tumors in 11 weeks postimplantation. The proportion of Ki-67-positive cells in tumors undergoing radiation was(71 ± 15)% compared with(25 ± 12)% in the nonirradiated group(P = 0.02). The number of MMP-2-positive areas and proportion of CD44-positive cells were also high in tumors receiving radiation, indicating great invasion and infiltration. Microvessel density analysis did not show a significant difference between nonirradiated and irradiated tumors. Taken together, we found that subcurative radiation significantly increased proliferation, invasion, and migration of primary GBM. Our study provides insights into possible mechanisms of treatment resistance following radiation therapy for GBM.
文摘We analyze the spin coincidence experiment considered by Bell in the derivation of Bells theorem. We solve the equation of motion for the spin system with a spin Hamiltonian, Hz, where the magnetic field is only in the z-direction. For the specific case of the coincidence experiment where the two magnets have the same orientation the Hamiltonian Hz commutes with the total spin Iz, which thus emerges as a constant of the motion. Bells argument is then that an observation of spin up at one magnet A necessarily implies spin down at the other B. For an isolated spin system A-B with classical translational degrees of freedom and an initial spin singlet state there is no force on the spin particles A and B. The spins are fully entangled but none of the spin particles A or B are deflected by the Stern-Gerlach magnets. This result is not compatible with Bells assumption that spin 1/2 particles are deected in a Stern-Gerlach device. Assuming a more realistic Hamiltonian Hz + Hx including a gradient in x direction the total Iz is not conserved and fully entanglement is not expected in this case. The conclusion is that Bells theorem is not applicable to spin coincidence measurement originally discussed by Bell.
文摘Aims: To investigate whether intravesical lower pH induces a micturition threshold reflex change of anaesthetized rats. Methods: Thirteen female rats, anaesthetized by a-chloralose and paralyzed by pancurone bromide were used for the experiments. The micturition threshold volume was determined by repeated cystometry. Simultaneously bladder afferent activities in four and efferent activities in seven rats were recorded from the exposed pelvic nerve to the bladder. Results: Cystometry with acidic saline at pH 3 and 4 induced a significant decrease in micturition threshold volume. The mean value of these groups of test showed that bladder compliance, micturition threshold pressure and maximal micturition contraction pressure were decreased significantly comparing with the controls. There were no changes in all other parameters recorded from afferent and efferent, e.g. afferent threshold volume, afferent or efferent activity at the micturition threshold and their peak activity at the micturition contraction. Conclusions: Acidic solution provoked sensitivity but reduced the force of the contractility to the urinary bladder. The effect was presented without a detectable change in the firing properties of bladder Ad mechanoreceptor afferents. It is proposed that proton sensitive bladder receptors with unmyelinated afferent were stimulated by the acidic solution and that the micturition reflex was facilitated by afferent inflow from such receptors. The findings were clinically important in choosing the medium for urodynamic examination.
文摘 The noncontact atomic force microscope(NCAFM)using frequency modulation(FM)technique can resolve the atomic features of the various surfaces.Recently,we found that the NC-AFM has a capability to identify or recognize atom species on a sample surface,if we can control an atomic species at the tip apex.That is,we succeeded in identification of Si and Ge atoms by imaging the Si/Ge intermixing Si(111)Surface using Si tip,where both of Si and Ge atoms are group IV elements[1].In the present experiments,we further investigate the capability of NC-AFM to identify the atom species under the surface.……
文摘b Département de Physique, Ecole Polythechnique Fédérale de Lausanne, EPFL, Lausanne, Switzerland c Department of Chemistry, Tsinghua University, Beijing 100084, China An excellent hole-transport material, 1,3-diphenyl-5-(9-phenanthryl)-2-pyrazoline (DPPhP) for OLEDs was studied. This compound not only offers high glass transition temperature (T g=96 ℃), good film forming ability, and high HOMO energy level, but also displays excellent hole-transport property. The electroluminescent device with a simple structure of ITO/DPPhP (60 nm)/AlQ (60 nm)/LiF (0.8 nm)/Al shows an external quantum efficiency as high as 1.6%.
基金Supported by National Key Basic Research Program of China(2015CB856700)National Natural Science Foundation of China(NSFC)(11235011,11335008,11425524,11625523,11635010)+13 种基金the Chinese Academy of Sciences(CAS)Large-Scale Scientific Facility Programthe CAS Center for Excellence in Particle Physics(CCEPP)Joint Large-Scale Scientific Facility Funds of the NSFCCAS(U1332201,U1532257,U1532258)CAS Key Research Program of Frontier Sciences(QYZDJ-SSW-SLH003,QYZDJ-SSW-SLH040)100 Talents Program of CASNational 1000 Talents Program of China,INPACShanghai Key Laboratory for Particle Physics and Cosmology,German Research Foundation DFG under Contracts Nos.Collaborative Research Center CRC 1044,FOR 2359Istituto Nazionale di Fisica Nucleare,Italy,Koninklijke Nederlandse Akademie van Wetenschappen(KNAW)(530-4CDP03)Ministry of Development of Turkey(DPT2006K-120470)National Science and Technology fundThe Swedish Research Council,U.S.Department of Energy(DEFG02-05ER41374,DE-SC-0010118,DE-SC-0010504,DE-SC-0012069)University of Groningen(RuG)and the Helmholtzzentrum fuer Schwerionenforschung GmbH(GSI)Darmstadt,WCU Program of National Research Foundation of Korea(R32-2008-000-10155-0)
文摘To investigate the nature of the Ψ（3770） resonance and to measure the cross section for e^＋e^-→DD, a cross-section scan data sample, distributed among 41 center-of-mass energy points from 3.73 to 3.89 GeV, was taken with the BESIII detector operated at the BEPCII collider in the year 2010. By analyzing the large angle Bhabha scattering events, we measure the integrated luminosity of the data sample at each center-of-mass energy point. The total integrated luminosity of the data sample is 76.16±0.04±0.61 pb^-1, where the first uncertainty is statistical and the second systematic.
文摘Background Recently congenital infection with Schistosoma japonicum (S. japonicum) has been domonstrated in pigs, rabbits, mice and dogs. We explored the rabbit as an animal model for the congenital infection of schistosomiasis japonica and assessed the effect of a congenital S. japonicum infection on the resistance of rabbit kittens to a postnatal challenge infection.Methods Sixteen pregnant New Zealand white rabbits were infected with a single dose of S. japonicum cercariae. The exposed animals were divided into three groups according to the gestation age at the time of infection. Diagnosis of prenatally acquired S. japonicum infection in the rabbit kittens was primarily based on serological tests in combination with parasitological and histopathological findings. Congenitally infected kittens were challenged percutaneously with 100 S. japonicum cercariae to assess the effect of a congenital S. japonicum infection on kitten resistance to a postnatal challenge infection.Results The overall prevalence of congenital infection in offspring of infected mothers was 20% (12/60). The congenital infection rate in group L (late gestation) was much higher than in group E (early gestation) and group M (mid-gestation) (P<0.05). After a postnatal challenge infection, prenatally infected kittens had a 54.66% worm reduction rate, 41.45% egg reduction rate, and 51.76% granuloma size reduction rate compared to nave kittens.Conclusions This study demonstrates the possibility of congenital infection of S. japonicum in rabbits and the resistance of congenitally infected kittens to a postnatal challenge infection. These results have important implications not only for epidemiological investigations, but also in designing government control programs for schistosomiasis.
基金Supported by National Key Basic Research Program of China(2015CB856700)National Natural Science Foundation of China(NSFC)(11235011,11335008,11425524,11625523,11635010)+8 种基金the Chinese Academy of Sciences(CAS)Large-Scale Scientific Facility Program,the CAS Center for Excellence in Particle Physics(CCEPP)Joint Large-Scale Scientific Facility Funds of the NSFC and CAS(U1332201,U1532257,U1532258)CAS Key Research Program of Frontier Sciences(QYZDJ-SSW-SLH003,QYZDJ-SSW-SLH040)100 Talents Program of CAS,National 1000 Talents Program of China,INPAC and Shanghai Key Laboratory for Particle Physics and Cosmology,German Research Foundation DFG under Contracts Nos.Collaborative Research Center CRC 1044,FOR 2359Istituto Nazionale di Fisica Nucleare,Italy,Koninklijke Nederlandse Akademie van Wetenschappen(KNAW)(530-4CDP03)Ministry of Development of Turkey(DPT2006K-120470)National Science and Technology fund,The Swedish Research Council,U.S.Department of Energy(DEFG02-05ER41374,DE-SC-0010118,DE-SC-0010504,DE-SC-0012069)University of Groningen(Ru G)and the Helmholtzzentrum fuer Schwerionenforschung Gmb H(GSI)Darmstadt,WCU Program of National Research Foundation of Korea(R32-2008-000-10155-0)
文摘We report new measurements of the cross sections for the production of DD final states at theψ（3770）resonance.Our data sample consists of an integrated luminosity of 2.93 fb^（-1）of e~＋e^-annihilation data produced by the BEPCⅡ collider and collected and analyzed with the BESⅢ detector.We exclusively reconstruct three D^0and six D~＋hadronic decay modes and use the ratio of the yield of fully reconstructed DD events（“double tags”）to the yield of all reconstructed D or D mesons（“single tags”）to determine the number of D^0 D^0and D~＋D^-events,benefiting from the cancellation of many systematic uncertainties.Combining these yields with an independent determination of the integrated luminosity of the data sample,we find the cross sections to beσ（e~＋e^-→D^0D^0）=（3.615±0.010±0.038）nb andσ（e~＋e^-→D~＋D^-）=（2.830±0.011±0.026）nb,where the uncertainties are statistical and systematic,respectively.
