Given the environmental pollution caused by petroleum-based plastics,finding alternative substitutes for sustainability has become critical.Polyhydroxybutyrate(PHB),a storage food material that is accumulated by sever...Given the environmental pollution caused by petroleum-based plastics,finding alternative substitutes for sustainability has become critical.Polyhydroxybutyrate(PHB),a storage food material that is accumulated by several bacteria,is biodegrad-able,safe,environment friendly and comparable to conventional plastics.However,scale-up is an issue due to high produc-tion cost.Substrate replacement using renewable,plentiful,sustainable and low-cost carbon sources derived from industrial waste facilitates waste reduction,while also enabling the synthesis of value-added products.In this context,inexpensive pulp and paper industrial waste as carbon source was exploited for production of PHB by using previously isolated(Source:hot springs of Manikarn,Himachal Pradesh,India)thermophilic bacteria Bacillus sonorensis NAM5 under optimized conditions in a fermenter.Production was done in a fermenter under optimized conditions(72 h of incubation at 50℃temperature and 7 pH)to enhance the accumulation of PHB.The bacterial strain was able to produce 5.28±0.11 gL^(-1)after 72 h of growth without any carbon and nitrogen source supplementation to the industrial effluent.The culture accumulated 66%PHB of cell dry weight(CDW).The produced polymer was characterized through FTIR,NMR and TGA.Additionally,bacteria-treated industrial wastewater was used for phytotoxicity assay on agriculturally important crops such as wheat,maize and mung,which exhibited considerable difference in growth parameters.展开更多
文摘Given the environmental pollution caused by petroleum-based plastics,finding alternative substitutes for sustainability has become critical.Polyhydroxybutyrate(PHB),a storage food material that is accumulated by several bacteria,is biodegrad-able,safe,environment friendly and comparable to conventional plastics.However,scale-up is an issue due to high produc-tion cost.Substrate replacement using renewable,plentiful,sustainable and low-cost carbon sources derived from industrial waste facilitates waste reduction,while also enabling the synthesis of value-added products.In this context,inexpensive pulp and paper industrial waste as carbon source was exploited for production of PHB by using previously isolated(Source:hot springs of Manikarn,Himachal Pradesh,India)thermophilic bacteria Bacillus sonorensis NAM5 under optimized conditions in a fermenter.Production was done in a fermenter under optimized conditions(72 h of incubation at 50℃temperature and 7 pH)to enhance the accumulation of PHB.The bacterial strain was able to produce 5.28±0.11 gL^(-1)after 72 h of growth without any carbon and nitrogen source supplementation to the industrial effluent.The culture accumulated 66%PHB of cell dry weight(CDW).The produced polymer was characterized through FTIR,NMR and TGA.Additionally,bacteria-treated industrial wastewater was used for phytotoxicity assay on agriculturally important crops such as wheat,maize and mung,which exhibited considerable difference in growth parameters.
