Objective:To identify the pathogenic gene of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn Hospital.Methods:A ...Objective:To identify the pathogenic gene of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn Hospital.Methods:A total of 104 samples of Staphylococcus were collected and 78 aureus samples were isolated from Zare Hospital patients from November 2016 to July 2017.All isolates were identified using a standard biochemical and laboratory methodology in accordance with CLSI principles,and disk agar diffusion antibiogram were performed to identify methicillin resistant colonies.Then the presence of the Panton-Valentine leukocidin gene was tested by PCR.Results:Of the methicillin-resistant Staphylococcus aureus samples,80%were negative for the Panton-Valentine leukocidin gene,and only 20%of the samples had Panton-Valentine leukocidin gene.Male and female patients showed no significant difference in the positivity rate of Panton-Valentine leukocidin gene(16.12%vs.33.33%)(P=0.25).Besides,there was no significant difference in bacterial resistance or susceptibility to antibiotics between samples with or without the Panton-Valentine leukocidin gene.Conclusions:In recent years,increased resistance has been a serious threat.The resistance or susceptibility of Staphylococcus aureus strains to different antibiotics is different in different geographical locations.展开更多
Managing Editor:Peng Lyu.Epstein-Barr virus(EBV),a member of the Herpesviridae family,is known for its involvement in gastric cancers.Because of its relatively high prevalence,The Cancer Genome Atlas classifies EBV-as...Managing Editor:Peng Lyu.Epstein-Barr virus(EBV),a member of the Herpesviridae family,is known for its involvement in gastric cancers.Because of its relatively high prevalence,The Cancer Genome Atlas classifies EBV-associated gastric cancers(EBVaGCs)as one of the four major molecular subtypes of gastric cancers.Because some studies have shown that EBV is present in all the cancerous cells and not in other surrounding mucosal cells,EBV infection is considered to have a causal role in EBVaGCs.1 In recent years,the pathogenic mechanism of EBVaGC has been the subject of intense investigation.It is believed that EBV contributes to gastric cancer through four major mechanisms.展开更多
基金This article was financially supported by Sari Branch,IslamicAzad University with grant number 410.
文摘Objective:To identify the pathogenic gene of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn Hospital.Methods:A total of 104 samples of Staphylococcus were collected and 78 aureus samples were isolated from Zare Hospital patients from November 2016 to July 2017.All isolates were identified using a standard biochemical and laboratory methodology in accordance with CLSI principles,and disk agar diffusion antibiogram were performed to identify methicillin resistant colonies.Then the presence of the Panton-Valentine leukocidin gene was tested by PCR.Results:Of the methicillin-resistant Staphylococcus aureus samples,80%were negative for the Panton-Valentine leukocidin gene,and only 20%of the samples had Panton-Valentine leukocidin gene.Male and female patients showed no significant difference in the positivity rate of Panton-Valentine leukocidin gene(16.12%vs.33.33%)(P=0.25).Besides,there was no significant difference in bacterial resistance or susceptibility to antibiotics between samples with or without the Panton-Valentine leukocidin gene.Conclusions:In recent years,increased resistance has been a serious threat.The resistance or susceptibility of Staphylococcus aureus strains to different antibiotics is different in different geographical locations.
文摘Managing Editor:Peng Lyu.Epstein-Barr virus(EBV),a member of the Herpesviridae family,is known for its involvement in gastric cancers.Because of its relatively high prevalence,The Cancer Genome Atlas classifies EBV-associated gastric cancers(EBVaGCs)as one of the four major molecular subtypes of gastric cancers.Because some studies have shown that EBV is present in all the cancerous cells and not in other surrounding mucosal cells,EBV infection is considered to have a causal role in EBVaGCs.1 In recent years,the pathogenic mechanism of EBVaGC has been the subject of intense investigation.It is believed that EBV contributes to gastric cancer through four major mechanisms.
