BACKGROUND Umbilical cord(UC)mesenchymal stem cell(MSC)transplantation is a potential therapeutic intervention for atherosclerotic vascular disease.Integrin beta 3(ITGB3)promotes cell migration in several cell types.H...BACKGROUND Umbilical cord(UC)mesenchymal stem cell(MSC)transplantation is a potential therapeutic intervention for atherosclerotic vascular disease.Integrin beta 3(ITGB3)promotes cell migration in several cell types.However,whether ITGBmodified MSCs can migrate to plaque sites in vivo and play an anti-atherosclerotic role remains unclear.AIM To investigate whether ITGB3-overexpressing MSCs(MSCs^(ITGB3))would exhibit improved homing efficacy in atherosclerosis.METHODS UC MSCs were isolated and expanded.Lentiviral vectors encoding ITGB3 or green fluorescent protein(GFP)as control were transfected into MSCs.Sixty male apolipoprotein E-/-mice were acquired from Beijing Vital River Lab Animal Technology Co.,Ltd and fed with a high-fat diet(HFD)for 12 wk to induce the formation of atherosclerotic lesions.These HFD-fed mice were randomly separated into three clusters.GFP-labeled MSCs(MSCs^(GFP))or MSCs^(ITGB3)were transplanted into the mice intravenously via the tail vein.Immunofluorescence staining,Oil red O staining,histological analyses,western blotting,enzymelinked immunosorbent assay,and quantitative real-time polymerase chain reaction were used for the analyses.RESULTS ITGB3 modified MSCs successfully differentiated into the“osteocyte”and“adipocyte”phenotypes and were characterized by positive expression(>91.3%)of CD29,CD73,and CD105 and negative expression(<1.35%)of CD34 and Human Leukocyte Antigen-DR.In a transwell assay,MSCs^(ITGB3)showed significantly faster migration than MSCsGFP.ITGB3 overexpression had no effects on MSC viability,differentiation,and secretion.Immunofluorescence staining revealed that ITGB3 overexpression substantially enhanced the homing of MSCs to plaque sites.Oil red O staining and histological analyses further confirmed the therapeutic effects of MSCs^(ITGB3),significantly reducing the plaque area.Enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction revealed that MSC^(ITGB3)transplantation considerably decreased the inflammatory response in pathological tissues by improving the dynamic equilibrium of pro-and anti-inflammatory cytokines.CONCLUSION These results showed that ITGB3 overexpression enhanced the MSC homing ability,providing a potential approach for MSC delivery to plaque sites,thereby optimizing their therapeutic effects.展开更多
Objective:To provide high-quality clinical evidence of the efficacy of Tibetan medicine Honghua Ruyi(HHRY)pills for endometriosis-associated dysmenorrhea.Methods:This study constitutes a multicenter,randomized,double-...Objective:To provide high-quality clinical evidence of the efficacy of Tibetan medicine Honghua Ruyi(HHRY)pills for endometriosis-associated dysmenorrhea.Methods:This study constitutes a multicenter,randomized,double-blind,placebo-controlled trial encompassing a three-menstrual cycle intervention followed by a three-menstrual cycle follow-up period.A total of 164 eligible females with endometriosis-associated dysmenorrhea were randomly divided into HHRY pills and placebo groups in a 1:1 ratio.The primary outcome included dysmenorrhea symptoms assessed using Visual Analog Scale(VAS)scores and quality of life,whereas the secondary outcome measures included the maximum VAS for non-menstrual pelvic pain,duration of pain episodes(in days),frequency and quantity of the consumption of ibuprofen sustained-release capsules(or other non-steroidal anti-inflammatory drugs),and days off work/study for staff/student due to dysmenorrhea,ovarian cyst,and/or pelvic nodule size.The safety was monitored throughout the treatment period.All the analyses were based on the intention-to-treat principle.For continuous outcomes,simple or multiple linear regressions were used to estimate the differences between the HHRY pills and placebo groups,with categorical data expressed as the number and percentage of occurrences.Differences were compared using the chi-square test or Fisher's exact test.The predefined analysis was adjusted for concomitant treatment,a variable considered to be associated with outcomes but unaffected by treatment allocation.Estimates of treatment effects were reported with 95%confidence intervals.Two-tailed P values≤.05 were considered statistically significant.Conclusion:Positive results from this trial,upon completion would provide robust evidence for the efficacy and safety of HHRY pills in treating dysmenorrhea in patients with endometriosis.展开更多
By employing many methods including distinguishing the land maps, interpreting the aerophotoes and Satellite photographes, consulting historical documents, investigating ancient cultural relics, making investigations ...By employing many methods including distinguishing the land maps, interpreting the aerophotoes and Satellite photographes, consulting historical documents, investigating ancient cultural relics, making investigations in the field, analysing the names and features of the place, and analysing the characteristics of the deposit, etc., a systematic study of the surface emerging palaeochannels on the South Coast Plain of Laizhou Bay has been made. Twenty five surface emerging palaeochannels were discovered and verified. According to the formation age, these palaeochannels were divided into two stages: six palaeochannels were formed in the Early and Middle Holocene, and nineteen palaeochannels were formed in the Late Holocene(the historical period). According to the landform features, the palaeochannels are divided into four categories: upland palaeochannel belts, strip shaped upland palaeochannels, trough shaped depression palaeochannels and ancient riverbeds. Among the four categories, the strip shaped upland palaeochannels are the main types. The swinging and movement of the channels helped the alluvial plains expand and cause the coastal ancient lakes to vanish in succession.展开更多
Objective:To explore the protective effect and potential mechanisms of danshen root(Salvia miltiorrhiza Bge.,S.miltiorrhiza) and its extracts for the treatment of diabetic nephropathy(DN).Methods:Preclinical studies o...Objective:To explore the protective effect and potential mechanisms of danshen root(Salvia miltiorrhiza Bge.,S.miltiorrhiza) and its extracts for the treatment of diabetic nephropathy(DN).Methods:Preclinical studies of S.miltiorrhiza and its extracts on DN were systematically searched in nine databases.The primary outcomes were blood glucose,kidney function,proteinuria,and renal histopathology.The secondary outcomes included the related mechanisms.The methodological quality of animal studies was assessed based on the risk of bias tool of the Systematic Review Centre for Laboratory Animal Experimentation(SYRCLE) for animal studies.Meta-analysis was performed using R software(version 4.1.2).Results:Twenty-nine animal experimental studies that met the eligibility criteria were included in this study.Compared to the control group,S.miltiorrhiza reduced the serum creatinine,blood urea nitrogen,24-h urine protein,24-h urine albumin,blood glucose,and kidney index(kidney weight/body weight),and alleviated renal pathological damage.In terms of the mechanism of action,compared to the control group,S.miltiorrhiza reduced the levels of transforming growth factor β1,collagen Ⅳ,malondialdehyde,tumor necrosis factor α,interleukin-6,and monocyte/macrophage(ED-1),and increased the levels of superoxide dismutase,glutathione peroxidase,nuclear factor E2-related factor 2,and heme oxygenase-1.Conclusion:The existing evidence shows that S.miltiorrhiza has beneficial effects on the animal model of DN,and its mechanism is mainly related to improving kidney fibrosis,oxidative stress,and inflammatory response.展开更多
Based on the theory of ecological organization,this paper analyzes the operation mechanism of chemical industry park(CIP)ecosystem by means of dynamic simulation.The research shows that the CIP ecosystem is a complex ...Based on the theory of ecological organization,this paper analyzes the operation mechanism of chemical industry park(CIP)ecosystem by means of dynamic simulation.The research shows that the CIP ecosystem is a complex ecological system whose operation mechanism includes two levels,namely individual enterprises and ecosystem.At the level of individual enterprise,there are competition,symbiosis,invasion and other interactions between enterprises in the CIP ecosystem.Through the pre-determined judgment of the competition effect coefficient and the symbiosis effect coefficient,we calculate how the enterprises influence each other,and then generate their respective operation paths,andfinally realize the long-term balance of the system.At the level of ecosystem,the CIP ecosystem makes self-organizing and self-adaptive adjustments and changes due to the changes of the exter-nal environment in policy,manpower,technology,capital,market,etc.,thus reaching the equilibrium state of coordinated operation and stable symbiosis with the external environment.展开更多
目的:系统评价宫瘤宁作为辅助疗法治疗子宫肌瘤的有效性和安全性。方法:检索数据库中国知网、万方、维普、PubMed、Cochrane Library、Embase、Web of Science以及中国临床试验注册系统、美国临床试验注册系统中有关宫瘤宁辅助治疗子宫...目的:系统评价宫瘤宁作为辅助疗法治疗子宫肌瘤的有效性和安全性。方法:检索数据库中国知网、万方、维普、PubMed、Cochrane Library、Embase、Web of Science以及中国临床试验注册系统、美国临床试验注册系统中有关宫瘤宁辅助治疗子宫肌瘤的临床随机对照试验。检索时限为各数据库建库至2023年3月20日。对符合纳入排除标准的研究采用“偏倚风险评估工具”进行文献质量评价,采用GRADE工具进行证据质量评价,应用RevMan5.3进行数据分析。结果:最终纳入20项研究,共1907例患者。15项研究的meta分析结果显示,宫瘤宁联合米非司酮在提高有效率方面优于单独使用米非司酮[RR=1.22,95%CI=(1.11,1.33),P<0.001]。因异质性较大,采用随机效应模型对纳入的研究进行合并。在缩小子宫肌瘤体积[MD=−4.09 cm^(3),95%CI(−5.16,−3.02),P<0.001],缩小子宫体积[MD=−23.22 cm^(3),95%C(I−29.10,−17.33),P<0.001],以及降低女性激素水平如孕激素水平、雌激素水平、促卵泡生成激素和促黄体生成激素等方面优于对照组(均P<0.001)。10项研究报告了不良事件。纳入研究的方法质量不高,存在中等偏倚风险。结论:与仅使用西医常规疗法相比,联合使用宫瘤宁在提高有效率方面有一定优势,在缩小子宫肌瘤体积、缩小子宫体积和降低女性激素水平等方面体现出一定优势趋势,但仍需更多质量高且设计严谨的研究进行验证。展开更多
Objective:To assess the quality of systematic reviews assessing the effects of traditional Chinese exercises on controlling blood indices,anthropometric indices,psychological indices,and quality of life in patients wi...Objective:To assess the quality of systematic reviews assessing the effects of traditional Chinese exercises on controlling blood indices,anthropometric indices,psychological indices,and quality of life in patients with diabetes.Methods:Systematic studies/meta-analyses of intervention with traditional Chinese exercises on diabetes mellitus were searched in the Pub Med,Web of Science,the China National Knowledge Infrastructure Databases(CNKI),Wan Fang Database,and Chinese Scientific Journal Database(VIP).Two researchers independently screened the studies and extracted the data.The methodology and quality of evidence of the included studies were assessed using A Measurement Tool to Assess Systematic Reviews2(AMSTAR-2)and Grading of Recommendations Assessment,Development and Evaluation(GRADE)criteria,respectively.Results:A total of 32 systematic reviews/meta-analyses were included in the present study.Of them,the methodological quality of 4 studies was graded as low,while that of the other 28 was graded as extremely low.The most common quality flaws in key items included a lack of preliminary proposals for systematic reviews,failure to explain the reasons for the inclusion criteria,failure to provide a list of excluded studies and reasons for exclusion,failure to report potential conflicts of interest,and inadequate assessment of publication bias.The quality of evidence for most of the 18 outcomes was subsequently graded as medium or low.Overall,the results of these studies indicated that Tai Chi,health qigong,and other traditional Chinese exercises lowered fasting blood glucose(FBG),2-h postprandial blood glucose(2hPBG),hemoglobin A_(1c)(HbA_(1c)),and body mass index,and relieved anxiety and depression in patients with diabetes.Conclusion:Our findings indicate that the methodological quality of systematic reviews related to traditional Chinese exercises in the diabetic population is generally low,and the quality of evidence is also relatively poor.Therefore,we suggest that the quality of systematic reviews and meta-analyses on traditional Chinese exercises for controlling diabetes mellitus needs to be improved.In the future,researchers should conduct higher-quality clinical studies with reference to the AMSTAR2 checklist and GRADE system.展开更多
Objective:To explore the characteristics of Xiao Chai Hu(XCH)decoction in randomized controlled trials(RCTs)using a bibliometric method.Methods:Seven databases including PubMed,Web of Science,Embase,Cochrane library,C...Objective:To explore the characteristics of Xiao Chai Hu(XCH)decoction in randomized controlled trials(RCTs)using a bibliometric method.Methods:Seven databases including PubMed,Web of Science,Embase,Cochrane library,China National Knowledge Infrastructure(CNKI),Chinese Scientific Journal Database(VIP)and Wanfang database were retrieved from inception to May 27,2022.In the study,XCH decoction(or modified)used alone or combined with conventional Western medicine as an intervention measure was included.The basic characteristics,funding support,relevant diseases,intervention methods,and adverse events(AEs)were analyzed.Data analysis was performed using SPSS 26.0 software.Results:A total of 813 RCTs were included,published from 1989 to 2022.There was only one Englishlanguage literature with the recent impact factor of 5.374.There were 147 studies were from Chineselanguage core journals,with the highest impact factor of 2.414.Only 6.15%of the literatures mentioned funding support.96.31%of the included literature reported the statistical significance of using XCH decoction.The diseases treated mainly included chronic hepatitis B(9.35%),cough variant asthma(5.66%),dizziness(5.54%),bile reflux gastritis(4.43%),and fever(4.18%).However,the overall research design of the included literature was poor,and large sample size,multicenter RCTs are needed.The incidence of AEs of XCH decoction alone was 8.86%,which was significantly lower than that of conventional Western medicine treatment.The combination of XCH decoction and conventional Western medicine treatment could reduce the incidence of AEs,and no serious adverse event was reported.Conclusion:Although the included studies show that XCH decoction is widely used,and has good efficacy and few AEs.Due to the low quality of the included RCTs,there may be some bias,and its rational use based on the specific conditions is recommended in clinical practice.展开更多
[Objectives] Caragana korshinskii resources are rich in Ordos with a large planting area, but they have not been fully and reasonably utilized. This study aims to effectively convert C. korshinskii into high-quality f...[Objectives] Caragana korshinskii resources are rich in Ordos with a large planting area, but they have not been fully and reasonably utilized. This study aims to effectively convert C. korshinskii into high-quality feed, improve the quality of milk and meat of herbivores and increase production. [Methods] The utilization of C. korshinskii in feed was studied in various aspects, the factors were selected, the level was determined, and the multi-factor experiment—orthogonal experiment was conducted. [Results] The effect on the growth of sheep was in the order of silage > C. korshinskii feed > corn stalk silage > hay meal, and the effect on the growth of goats was in the order of hay meal > C. korshinskii feed > silage > corn stalk silage. [Conclusions] From the analysis of the range results, the best level combination was selected: in different growing seasons, C. korshinskii branch and leaf silage in full-bloom stage had the greatest effect on the growth of sheep and goats, and the fattening effect was the best;among the different silage methods of C. korshinskii, the C. korshinskii—corn stalk mixture silage had a good feeding effect for sheep and goats.展开更多
BACKGROUND Chronic hepatitis B virus(HBV)infection is often associated with increased lipid deposition in hepatocytes.However,when combined with non-alcoholic fatty liver disease or hyperlipidemia,it tends to have a l...BACKGROUND Chronic hepatitis B virus(HBV)infection is often associated with increased lipid deposition in hepatocytes.However,when combined with non-alcoholic fatty liver disease or hyperlipidemia,it tends to have a lower HBV deoxyribonucleic acid(DNA)load.The relationship between lipid metabolism and HBV DNA replication and its underlying mechanisms are not well understood.AIM To investigate the relationship between lipid metabolism and HBV DNA replication and its underlying mechanisms.METHODS 1603 HBsAg-seropositive patients were included in the study.We first explored the relationship between patients'lipid levels,hepatic steatosis,and HBV DNA load.Also,we constructed an HBV infection combined with a hepatic steatosis cell model in vitro by fatty acid stimulation of HepG2.2.15 cells to validate the effect of lipid metabolism on HBV DNA replication in vitro.By knocking down and overexpressing Plin2,we observed whether Plin2 regulates autophagy and HBV replication.By inhibiting both Plin2 and cellular autophagy under high lipid stimulation,we examined whether the Plin2-autophagy pathway regulates HBV replication.RESULTS The results revealed that serum triglyceride levels,high-density lipoprotein levels,and hepatic steatosis ratio were significantly lower in the HBV-DNA high load group.Logistic regression analysis indicated that hepatic steatosis and serum triglyceride levels were negatively correlated with HBV-DNA load.Stratified analysis by HBeAg showed significant negative correlations between HBV-DNA load and hepatic steatosis ratio in both HBeAgpositive and HBeAg-negative groups.An in vitro cell model was developed by stimulating HepG2.2.15 cells with palmitic acid and oleic acid to study the relationship between HBV-DNA load and lipid metabolism.The results of the in vitro experiments suggested that fatty acid treatment increased lipid droplet deposition and decreased the expression of cell supernatant HBsAg,HBeAg,and HBV DNA load.Western blot and polymerase chain reaction analysis showed that fatty acid stimulation significantly induced Plin2 protein expression and inhibited the expression of hepatocyte autophagy proteins.Inhibition of Plin2 protein expression under fatty acid stimulation reversed the reduction in HBsAg and HBeAg expression and HBV DNA load induced by fatty acid stimulation and the inhibition of cellular autophagy.Knocking down Plin2 and blocking autophagy with 3-methyladenine(3-MA)inhibited HBV DNA replication.CONCLUSION In conclusion,lipid metabolism is a significant factor affecting HBV load in patients with HBV infection.The in vitro experiments established that fatty acid stimulation inhibits HBV replication via the Plin2-autophagy pathway.展开更多
基金National Natural Science Foundation of China,No.82100301Key Science and Technology Research Program of Hebei Provincial Department of Health,No,20221014.
文摘BACKGROUND Umbilical cord(UC)mesenchymal stem cell(MSC)transplantation is a potential therapeutic intervention for atherosclerotic vascular disease.Integrin beta 3(ITGB3)promotes cell migration in several cell types.However,whether ITGBmodified MSCs can migrate to plaque sites in vivo and play an anti-atherosclerotic role remains unclear.AIM To investigate whether ITGB3-overexpressing MSCs(MSCs^(ITGB3))would exhibit improved homing efficacy in atherosclerosis.METHODS UC MSCs were isolated and expanded.Lentiviral vectors encoding ITGB3 or green fluorescent protein(GFP)as control were transfected into MSCs.Sixty male apolipoprotein E-/-mice were acquired from Beijing Vital River Lab Animal Technology Co.,Ltd and fed with a high-fat diet(HFD)for 12 wk to induce the formation of atherosclerotic lesions.These HFD-fed mice were randomly separated into three clusters.GFP-labeled MSCs(MSCs^(GFP))or MSCs^(ITGB3)were transplanted into the mice intravenously via the tail vein.Immunofluorescence staining,Oil red O staining,histological analyses,western blotting,enzymelinked immunosorbent assay,and quantitative real-time polymerase chain reaction were used for the analyses.RESULTS ITGB3 modified MSCs successfully differentiated into the“osteocyte”and“adipocyte”phenotypes and were characterized by positive expression(>91.3%)of CD29,CD73,and CD105 and negative expression(<1.35%)of CD34 and Human Leukocyte Antigen-DR.In a transwell assay,MSCs^(ITGB3)showed significantly faster migration than MSCsGFP.ITGB3 overexpression had no effects on MSC viability,differentiation,and secretion.Immunofluorescence staining revealed that ITGB3 overexpression substantially enhanced the homing of MSCs to plaque sites.Oil red O staining and histological analyses further confirmed the therapeutic effects of MSCs^(ITGB3),significantly reducing the plaque area.Enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction revealed that MSC^(ITGB3)transplantation considerably decreased the inflammatory response in pathological tissues by improving the dynamic equilibrium of pro-and anti-inflammatory cytokines.CONCLUSION These results showed that ITGB3 overexpression enhanced the MSC homing ability,providing a potential approach for MSC delivery to plaque sites,thereby optimizing their therapeutic effects.
基金supported by the National Natural Science Foundation of China(81830115).
文摘Objective:To provide high-quality clinical evidence of the efficacy of Tibetan medicine Honghua Ruyi(HHRY)pills for endometriosis-associated dysmenorrhea.Methods:This study constitutes a multicenter,randomized,double-blind,placebo-controlled trial encompassing a three-menstrual cycle intervention followed by a three-menstrual cycle follow-up period.A total of 164 eligible females with endometriosis-associated dysmenorrhea were randomly divided into HHRY pills and placebo groups in a 1:1 ratio.The primary outcome included dysmenorrhea symptoms assessed using Visual Analog Scale(VAS)scores and quality of life,whereas the secondary outcome measures included the maximum VAS for non-menstrual pelvic pain,duration of pain episodes(in days),frequency and quantity of the consumption of ibuprofen sustained-release capsules(or other non-steroidal anti-inflammatory drugs),and days off work/study for staff/student due to dysmenorrhea,ovarian cyst,and/or pelvic nodule size.The safety was monitored throughout the treatment period.All the analyses were based on the intention-to-treat principle.For continuous outcomes,simple or multiple linear regressions were used to estimate the differences between the HHRY pills and placebo groups,with categorical data expressed as the number and percentage of occurrences.Differences were compared using the chi-square test or Fisher's exact test.The predefined analysis was adjusted for concomitant treatment,a variable considered to be associated with outcomes but unaffected by treatment allocation.Estimates of treatment effects were reported with 95%confidence intervals.Two-tailed P values≤.05 were considered statistically significant.Conclusion:Positive results from this trial,upon completion would provide robust evidence for the efficacy and safety of HHRY pills in treating dysmenorrhea in patients with endometriosis.
基金Under the auspices of the National Natural Foundation of China (49871011).
文摘By employing many methods including distinguishing the land maps, interpreting the aerophotoes and Satellite photographes, consulting historical documents, investigating ancient cultural relics, making investigations in the field, analysing the names and features of the place, and analysing the characteristics of the deposit, etc., a systematic study of the surface emerging palaeochannels on the South Coast Plain of Laizhou Bay has been made. Twenty five surface emerging palaeochannels were discovered and verified. According to the formation age, these palaeochannels were divided into two stages: six palaeochannels were formed in the Early and Middle Holocene, and nineteen palaeochannels were formed in the Late Holocene(the historical period). According to the landform features, the palaeochannels are divided into four categories: upland palaeochannel belts, strip shaped upland palaeochannels, trough shaped depression palaeochannels and ancient riverbeds. Among the four categories, the strip shaped upland palaeochannels are the main types. The swinging and movement of the channels helped the alluvial plains expand and cause the coastal ancient lakes to vanish in succession.
基金This study was supported by the National Natural Science Foundation of China(81373831)the National Key Research and Development Program of the Ministry of Science and Technology of China(2018YFC1704304).
文摘Objective:To explore the protective effect and potential mechanisms of danshen root(Salvia miltiorrhiza Bge.,S.miltiorrhiza) and its extracts for the treatment of diabetic nephropathy(DN).Methods:Preclinical studies of S.miltiorrhiza and its extracts on DN were systematically searched in nine databases.The primary outcomes were blood glucose,kidney function,proteinuria,and renal histopathology.The secondary outcomes included the related mechanisms.The methodological quality of animal studies was assessed based on the risk of bias tool of the Systematic Review Centre for Laboratory Animal Experimentation(SYRCLE) for animal studies.Meta-analysis was performed using R software(version 4.1.2).Results:Twenty-nine animal experimental studies that met the eligibility criteria were included in this study.Compared to the control group,S.miltiorrhiza reduced the serum creatinine,blood urea nitrogen,24-h urine protein,24-h urine albumin,blood glucose,and kidney index(kidney weight/body weight),and alleviated renal pathological damage.In terms of the mechanism of action,compared to the control group,S.miltiorrhiza reduced the levels of transforming growth factor β1,collagen Ⅳ,malondialdehyde,tumor necrosis factor α,interleukin-6,and monocyte/macrophage(ED-1),and increased the levels of superoxide dismutase,glutathione peroxidase,nuclear factor E2-related factor 2,and heme oxygenase-1.Conclusion:The existing evidence shows that S.miltiorrhiza has beneficial effects on the animal model of DN,and its mechanism is mainly related to improving kidney fibrosis,oxidative stress,and inflammatory response.
文摘Based on the theory of ecological organization,this paper analyzes the operation mechanism of chemical industry park(CIP)ecosystem by means of dynamic simulation.The research shows that the CIP ecosystem is a complex ecological system whose operation mechanism includes two levels,namely individual enterprises and ecosystem.At the level of individual enterprise,there are competition,symbiosis,invasion and other interactions between enterprises in the CIP ecosystem.Through the pre-determined judgment of the competition effect coefficient and the symbiosis effect coefficient,we calculate how the enterprises influence each other,and then generate their respective operation paths,andfinally realize the long-term balance of the system.At the level of ecosystem,the CIP ecosystem makes self-organizing and self-adaptive adjustments and changes due to the changes of the exter-nal environment in policy,manpower,technology,capital,market,etc.,thus reaching the equilibrium state of coordinated operation and stable symbiosis with the external environment.
基金supported by the Fundamental Research Funds for the Central Universities(2022-JYB-JBZR-026)the National Natural Science Foundation of China(81873211)。
文摘Objective:To assess the quality of systematic reviews assessing the effects of traditional Chinese exercises on controlling blood indices,anthropometric indices,psychological indices,and quality of life in patients with diabetes.Methods:Systematic studies/meta-analyses of intervention with traditional Chinese exercises on diabetes mellitus were searched in the Pub Med,Web of Science,the China National Knowledge Infrastructure Databases(CNKI),Wan Fang Database,and Chinese Scientific Journal Database(VIP).Two researchers independently screened the studies and extracted the data.The methodology and quality of evidence of the included studies were assessed using A Measurement Tool to Assess Systematic Reviews2(AMSTAR-2)and Grading of Recommendations Assessment,Development and Evaluation(GRADE)criteria,respectively.Results:A total of 32 systematic reviews/meta-analyses were included in the present study.Of them,the methodological quality of 4 studies was graded as low,while that of the other 28 was graded as extremely low.The most common quality flaws in key items included a lack of preliminary proposals for systematic reviews,failure to explain the reasons for the inclusion criteria,failure to provide a list of excluded studies and reasons for exclusion,failure to report potential conflicts of interest,and inadequate assessment of publication bias.The quality of evidence for most of the 18 outcomes was subsequently graded as medium or low.Overall,the results of these studies indicated that Tai Chi,health qigong,and other traditional Chinese exercises lowered fasting blood glucose(FBG),2-h postprandial blood glucose(2hPBG),hemoglobin A_(1c)(HbA_(1c)),and body mass index,and relieved anxiety and depression in patients with diabetes.Conclusion:Our findings indicate that the methodological quality of systematic reviews related to traditional Chinese exercises in the diabetic population is generally low,and the quality of evidence is also relatively poor.Therefore,we suggest that the quality of systematic reviews and meta-analyses on traditional Chinese exercises for controlling diabetes mellitus needs to be improved.In the future,researchers should conduct higher-quality clinical studies with reference to the AMSTAR2 checklist and GRADE system.
基金supported by a Project of National Administration of Traditional Chinese Medicine(TB2021011)“Excellence Program”of the Beijing University of Chinese Medicine.
文摘Objective:To explore the characteristics of Xiao Chai Hu(XCH)decoction in randomized controlled trials(RCTs)using a bibliometric method.Methods:Seven databases including PubMed,Web of Science,Embase,Cochrane library,China National Knowledge Infrastructure(CNKI),Chinese Scientific Journal Database(VIP)and Wanfang database were retrieved from inception to May 27,2022.In the study,XCH decoction(or modified)used alone or combined with conventional Western medicine as an intervention measure was included.The basic characteristics,funding support,relevant diseases,intervention methods,and adverse events(AEs)were analyzed.Data analysis was performed using SPSS 26.0 software.Results:A total of 813 RCTs were included,published from 1989 to 2022.There was only one Englishlanguage literature with the recent impact factor of 5.374.There were 147 studies were from Chineselanguage core journals,with the highest impact factor of 2.414.Only 6.15%of the literatures mentioned funding support.96.31%of the included literature reported the statistical significance of using XCH decoction.The diseases treated mainly included chronic hepatitis B(9.35%),cough variant asthma(5.66%),dizziness(5.54%),bile reflux gastritis(4.43%),and fever(4.18%).However,the overall research design of the included literature was poor,and large sample size,multicenter RCTs are needed.The incidence of AEs of XCH decoction alone was 8.86%,which was significantly lower than that of conventional Western medicine treatment.The combination of XCH decoction and conventional Western medicine treatment could reduce the incidence of AEs,and no serious adverse event was reported.Conclusion:Although the included studies show that XCH decoction is widely used,and has good efficacy and few AEs.Due to the low quality of the included RCTs,there may be some bias,and its rational use based on the specific conditions is recommended in clinical practice.
基金Supported by Demonstration Project of Forestry and Grassland Science and Technology Popularization by Central Finance[NeiLinCaoKeTui(2021)]Major Project of"Revitalizing Mongolia through Science and Technology"(KJXM-EEDS-2020006)Major Science and Technology Project of Inner Mongolia Autonomous Region(2019ZD007)。
文摘[Objectives] Caragana korshinskii resources are rich in Ordos with a large planting area, but they have not been fully and reasonably utilized. This study aims to effectively convert C. korshinskii into high-quality feed, improve the quality of milk and meat of herbivores and increase production. [Methods] The utilization of C. korshinskii in feed was studied in various aspects, the factors were selected, the level was determined, and the multi-factor experiment—orthogonal experiment was conducted. [Results] The effect on the growth of sheep was in the order of silage > C. korshinskii feed > corn stalk silage > hay meal, and the effect on the growth of goats was in the order of hay meal > C. korshinskii feed > silage > corn stalk silage. [Conclusions] From the analysis of the range results, the best level combination was selected: in different growing seasons, C. korshinskii branch and leaf silage in full-bloom stage had the greatest effect on the growth of sheep and goats, and the fattening effect was the best;among the different silage methods of C. korshinskii, the C. korshinskii—corn stalk mixture silage had a good feeding effect for sheep and goats.
文摘BACKGROUND Chronic hepatitis B virus(HBV)infection is often associated with increased lipid deposition in hepatocytes.However,when combined with non-alcoholic fatty liver disease or hyperlipidemia,it tends to have a lower HBV deoxyribonucleic acid(DNA)load.The relationship between lipid metabolism and HBV DNA replication and its underlying mechanisms are not well understood.AIM To investigate the relationship between lipid metabolism and HBV DNA replication and its underlying mechanisms.METHODS 1603 HBsAg-seropositive patients were included in the study.We first explored the relationship between patients'lipid levels,hepatic steatosis,and HBV DNA load.Also,we constructed an HBV infection combined with a hepatic steatosis cell model in vitro by fatty acid stimulation of HepG2.2.15 cells to validate the effect of lipid metabolism on HBV DNA replication in vitro.By knocking down and overexpressing Plin2,we observed whether Plin2 regulates autophagy and HBV replication.By inhibiting both Plin2 and cellular autophagy under high lipid stimulation,we examined whether the Plin2-autophagy pathway regulates HBV replication.RESULTS The results revealed that serum triglyceride levels,high-density lipoprotein levels,and hepatic steatosis ratio were significantly lower in the HBV-DNA high load group.Logistic regression analysis indicated that hepatic steatosis and serum triglyceride levels were negatively correlated with HBV-DNA load.Stratified analysis by HBeAg showed significant negative correlations between HBV-DNA load and hepatic steatosis ratio in both HBeAgpositive and HBeAg-negative groups.An in vitro cell model was developed by stimulating HepG2.2.15 cells with palmitic acid and oleic acid to study the relationship between HBV-DNA load and lipid metabolism.The results of the in vitro experiments suggested that fatty acid treatment increased lipid droplet deposition and decreased the expression of cell supernatant HBsAg,HBeAg,and HBV DNA load.Western blot and polymerase chain reaction analysis showed that fatty acid stimulation significantly induced Plin2 protein expression and inhibited the expression of hepatocyte autophagy proteins.Inhibition of Plin2 protein expression under fatty acid stimulation reversed the reduction in HBsAg and HBeAg expression and HBV DNA load induced by fatty acid stimulation and the inhibition of cellular autophagy.Knocking down Plin2 and blocking autophagy with 3-methyladenine(3-MA)inhibited HBV DNA replication.CONCLUSION In conclusion,lipid metabolism is a significant factor affecting HBV load in patients with HBV infection.The in vitro experiments established that fatty acid stimulation inhibits HBV replication via the Plin2-autophagy pathway.
