Background: Centrosomal protein 78(CEP78) has been characterized as a component of the centrosome required for the regulation of centrosome-related events during the cell cycle, but its role in human cancers remains u...Background: Centrosomal protein 78(CEP78) has been characterized as a component of the centrosome required for the regulation of centrosome-related events during the cell cycle, but its role in human cancers remains unclear. This study aimed to investigate the role and the clinical value of CEP78 in colorectal cancer(CRC).Methods: Quantitative real-time polymerase chain reaction(q RT-PCR) and immunohistochemistry were performed to examine CEP78 expression in CRC tissues and adjacent noncancerous tissues. The association between CEP78 expression and clinical outcomes of CRC patients was determined. The effect of CEP78 on cell growth was examined in vitro by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay, colony formation, and flow cytometry assays and in vivo using a nude mouse model.Results: The expression level of CEP78 was significantly lower in tumor tissues than in the adjacent normal tissues(P < 0.01). Low CEP78 expression was significantly associated with poor differentiation(P etastasis(P = 0.003), large tumor size(P = 0.017), lymphatic mtient= 0.034), distant metastasis(P s with low CEP78 expression h= 0.029), and advanced stage(P Meier analysis indicated that paad shorter survival than those wit= 0.011). Kaplan–h high CEP78 expression(P < 0.01). Overexpression of CEP78 in CRC cells significantly reduced cell viability and colony formation in vitro and halted tumor growth in vivo. Further study showed that CEP78 reintroduction in CRC cells resulted in G2/M phase arrest rather than cell apoptosis.Conclusions: CEP78 might function as a tumor suppressor and serve as a novel prognostic marker in CRC.展开更多
Lithium-sulfur(Li-S)batteries with high theoretical specific energy are considered to be one of the highly promising next-generation energy storage systems.However,the shuttle effect of lithium polysulfides(LiPSs)and ...Lithium-sulfur(Li-S)batteries with high theoretical specific energy are considered to be one of the highly promising next-generation energy storage systems.However,the shuttle effect of lithium polysulfides(LiPSs)and the interfacial instability of Li anodes have seriously hindered the practical application of Li-S batteries.Optimizing the electrolyte composition with additives can significantly improve the battery performance and has attracted great attention.Herein,we propose an organometallic salt,i.e.,nickel bromide dimethoxyethane(NiBr_(2)DME),as an electrolyte additive,which serves as the dual function of regulating LiPSs redox and synchronously stabilizing Li anodes.We reveal that NiBr_(2)DME can interact with LiPSs via Ni-S and Li-Br bonds,and accelerate the mutual transformation of LiPSs,thus reducing the accumulation of LiPSs in the electrolyte.In addition,NiBr_(2)DME can form a stable LiBr-containing interfacial layer on the Li metal surface,and promote the uniform electrodeposition of Li^(+)ions,and inhibit the formation of Li dendrites.Thus,Li-S batteries with a concentration of 0.5 mmol L^(-1)NiBr_(2)DME show an initial capacity of 919.8 mAh g^(-1)at 0.2 C,and a high capacity retention of 89.3%after 100 cycles.Even at the 4 C rate,a high discharge capacity of 602.9 mAh g^(-1)is achieved.Surprisingly,the good cycling performance is maintained under poor electrolyte conditions with sulfur loading of 4.8 mg cm^(-2)and electrlyte/sulfur ratio of 5µL mg^(-1).This work provides a positive solution to achieve the suppression of shuttle effect,the regulation of LiPSs redox and the stabilization of Li anodes.展开更多
文摘Background: Centrosomal protein 78(CEP78) has been characterized as a component of the centrosome required for the regulation of centrosome-related events during the cell cycle, but its role in human cancers remains unclear. This study aimed to investigate the role and the clinical value of CEP78 in colorectal cancer(CRC).Methods: Quantitative real-time polymerase chain reaction(q RT-PCR) and immunohistochemistry were performed to examine CEP78 expression in CRC tissues and adjacent noncancerous tissues. The association between CEP78 expression and clinical outcomes of CRC patients was determined. The effect of CEP78 on cell growth was examined in vitro by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay, colony formation, and flow cytometry assays and in vivo using a nude mouse model.Results: The expression level of CEP78 was significantly lower in tumor tissues than in the adjacent normal tissues(P < 0.01). Low CEP78 expression was significantly associated with poor differentiation(P etastasis(P = 0.003), large tumor size(P = 0.017), lymphatic mtient= 0.034), distant metastasis(P s with low CEP78 expression h= 0.029), and advanced stage(P Meier analysis indicated that paad shorter survival than those wit= 0.011). Kaplan–h high CEP78 expression(P < 0.01). Overexpression of CEP78 in CRC cells significantly reduced cell viability and colony formation in vitro and halted tumor growth in vivo. Further study showed that CEP78 reintroduction in CRC cells resulted in G2/M phase arrest rather than cell apoptosis.Conclusions: CEP78 might function as a tumor suppressor and serve as a novel prognostic marker in CRC.
基金supported by the Jiangxi Province Leading Talent Project for Academic and Technical Leaders in Major Disciplines(20213BCJ22024)National Natural Science Foundation of China(22269013,22263009 and 21863006)+1 种基金Natural Science Foundation of Jiangxi Province(20224ACB213001 and 20212BBE53051)Elite Scientists Sponsorship Program by Jiangxi Association for Science and Technology(2023QT07)。
文摘Lithium-sulfur(Li-S)batteries with high theoretical specific energy are considered to be one of the highly promising next-generation energy storage systems.However,the shuttle effect of lithium polysulfides(LiPSs)and the interfacial instability of Li anodes have seriously hindered the practical application of Li-S batteries.Optimizing the electrolyte composition with additives can significantly improve the battery performance and has attracted great attention.Herein,we propose an organometallic salt,i.e.,nickel bromide dimethoxyethane(NiBr_(2)DME),as an electrolyte additive,which serves as the dual function of regulating LiPSs redox and synchronously stabilizing Li anodes.We reveal that NiBr_(2)DME can interact with LiPSs via Ni-S and Li-Br bonds,and accelerate the mutual transformation of LiPSs,thus reducing the accumulation of LiPSs in the electrolyte.In addition,NiBr_(2)DME can form a stable LiBr-containing interfacial layer on the Li metal surface,and promote the uniform electrodeposition of Li^(+)ions,and inhibit the formation of Li dendrites.Thus,Li-S batteries with a concentration of 0.5 mmol L^(-1)NiBr_(2)DME show an initial capacity of 919.8 mAh g^(-1)at 0.2 C,and a high capacity retention of 89.3%after 100 cycles.Even at the 4 C rate,a high discharge capacity of 602.9 mAh g^(-1)is achieved.Surprisingly,the good cycling performance is maintained under poor electrolyte conditions with sulfur loading of 4.8 mg cm^(-2)and electrlyte/sulfur ratio of 5µL mg^(-1).This work provides a positive solution to achieve the suppression of shuttle effect,the regulation of LiPSs redox and the stabilization of Li anodes.
