The process of wound healing is routinely evaluated by histological evaluation in the clinic,which may cause scarring and secondary injury.Reflectance confocal microscopy(RCM)represents a noninvasive,real-time imaging...The process of wound healing is routinely evaluated by histological evaluation in the clinic,which may cause scarring and secondary injury.Reflectance confocal microscopy(RCM)represents a noninvasive,real-time imaging technique that allows in vivo evaluation of the skin.Traditional RCM was wide-probe-based,which limited its application on uneven and covered skin.In this study,we report the development of a portable reflectance confocal microscope(PRCM)in which all components were assembled in a handheld shell.Although the size and weight of the PRCM were reduced based on the use of a microelectromechanical system,the resolution was kept at 0.91μm,and the field of view of the system was 343μm×532μm.When used in vivo,the PRCM was able to visualize cellular and nuclear morphology for both mouse and human skin.PRCM evaluations were then performed on wounds after topically applied mesenchymal stem cells(MSCs)or saline treatment.The PRCM allowed visualization of the formation of collagen bundles,re-epithelization from the wound edge to the wound bed,and hair follicle regeneration,which were consistent with histological evaluations.Therefore,we offer new insights into monitoring the effects of topically applied MSCs on the process of wound healing by using PRCM.This study illustrates that the newly developed PRCM represents a promising device for real-time,noninvasive monitoring of the dynamic process of wound healing,which demonstrates its potential to diagnose,monitor,or predict disease in clinical wound therapy.展开更多
Okra(Abelmoschus esculentus)is an important vegetable crop with high nutritional value.However,the mechanism underlying its high nutrient content remains poorly understood.Here,we present a chromosome-scale genome of ...Okra(Abelmoschus esculentus)is an important vegetable crop with high nutritional value.However,the mechanism underlying its high nutrient content remains poorly understood.Here,we present a chromosome-scale genome of okra with a size of 1.19 Gb.Comparative genomics analysis revealed the phylogenetic status of A.esculentus,as well as whole-genome duplication(WGD)events that have occurred widely across the Malvaceae species.We found that okra has experienced three additional WGDs compared with the diploid cotton Gossypium raimondii,resulting in a large chromosome number(2n=130).After three WGDs,okra has undergone extensive genomic deletions and retained substantial numbers of genes related to secondary metabolite biosynthesis and environmental adaptation,resulting in significant differences between okra and G.raimondii in the gene families related to cellulose synthesis.Combining transcriptomic and metabolomic analysis,we revealed the relationship between gene expression and metabolite content change across different okra developmental stages.Furthermore,the sinapic acid/S-lignin biosynthesis-related gene families have experienced remarkable expansion in okra,and the expression of key enzymes involved in the sinapic acid/S-lignin biosynthesis pathway vary greatly across developmental periods,which partially explains the differences in metabolite content across the different stages.Our study gains insights into the comprehensive evolutionary history of Malvaceae species and the genetic basis that underlies the nutrient content changes in okra,which will facilitate the functional study and genetic improvement of okra varieties.展开更多
Personalized search utilizes user preferences to optimize search results,and most existing studies obtain user preferences by analyzing user behaviors in search engines that provide click-through data.However,the beha...Personalized search utilizes user preferences to optimize search results,and most existing studies obtain user preferences by analyzing user behaviors in search engines that provide click-through data.However,the behavioral data are noisy because users often clicked some irrelevant documents to find their required information,and the new user cold start issue represents a serious problem,greatly reducing the performance of personalized search.This paper attempts to utilize online social network data to obtain user preferences that can be used to personalize search results,mine the knowledge of user interests,user influence and user relationships from online social networks,and use this knowledge to optimize the results returned by search engines.The proposed model is based on a holonic multiagent system that improves the adaptability and scalability of the model.The experimental results show that utilizing online social network data to implement personalized search is feasible and that online social network data are significant for personalized search.展开更多
Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired.Recently,topical stem cell therapy has been realized as a promising strategy for wound healing and skin reg...Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired.Recently,topical stem cell therapy has been realized as a promising strategy for wound healing and skin regeneration.However,stem cells must be administrated uniformly to the wound area,otherwise treatment will be ineffective,which has been a limitation of current administration methods.Specifically,the delivery pressure and nozzle features of most clinical cell spray devices are unknown,which may significantly affect the viability of sprayed cells and their capacity for proliferation.Herein,we developed a novel pneumatically assisted atomization device(PAAD)in which cell suspensions were uniformly atomized at a low delivery pressure.We optimized the applied fluidic pressure and air pressure to maximize cell survival and function for the application of multiple cell types,while ensuring uniform dispersal across the wound site.Moreover,we found that the application of sprayed umbilical cord-derived mesenchymal stem cells to wound sites significantly accelerated wound healing and promoted appendage regeneration on an excisional cutaneous wound model.Overall,the novel PAAD system delivered living cells uniformly and maintained the viability and differentiation of sprayed cells,strongly suggesting its potential for application in clinical cell therapy,especially for large,irregular,and severe skin wounds.展开更多
User interest mining on Sina Weibo is the basis of personalized recommendations,advertising,marketing promotions,and other tasks.Although great progress has been made in this area,previous studies have ignored the dif...User interest mining on Sina Weibo is the basis of personalized recommendations,advertising,marketing promotions,and other tasks.Although great progress has been made in this area,previous studies have ignored the differences among users:the varied behaviors and habits that lead to unique user data characteristics.It is unreasonable to use a single strategy to mine interests from such varied user data.Therefore,this paper proposes an adaptive model for user interest mining based on a multi-agent system whose input includes self-descriptive user data,microblogs and correlations.This method has the ability to select the appropriate strategy based on each user’s data characteristics.The experimental results show that the proposed method performs better than the baselines.展开更多
Common buckwheat(Fagopyrum esculentum)and Tartary buckwheat(Fagopyrum tataricum),the two most widely cultivated buckwheat species,differ greatly in flavonoid content and reproductive mode.Here,we report the first high...Common buckwheat(Fagopyrum esculentum)and Tartary buckwheat(Fagopyrum tataricum),the two most widely cultivated buckwheat species,differ greatly in flavonoid content and reproductive mode.Here,we report the first high-quality and chromosome-level genome assembly of common buckwheat with 1.2 Gb.Comparative genomic analysis revealed that common buckwheat underwent a burst of long terminal repeat retrotransposons insertion accompanied by numerous large chromosome rearrangements after divergence from Tartary buckwheat.Moreover,multiple gene families involved in stress tolerance and flavonoid biosynthesis such as multidrug and toxic compound extrusion(MATE)and chalcone synthase(CHS)underwent significant expansion in buckwheat,especially in common buckwheat.Integrated multi-omics analysis identified high expression of catechin biosynthesis-related genes in flower and seed in common buckwheat and high expression of rutin biosynthesis-related genes in seed in Tartary buckwheat as being important for the differences in flavonoid type and content between these buckwheat species.We also identified a candidate key rutindegrading enzyme gene(Ft8.2377)that was highly expressed in Tartary buckwheat seed.In addition,we identified a haplotype-resolved candidate locus containing many genes reportedly associated with the development of flower and pollen,which was potentially related to self-incompatibility in common buckwheat.Our study provides important resources facilitating future functional genomics-related research of flavonoid biosynthesis and selfincompatibility in buckwheat.展开更多
基金the National Key Research andDevelopment Program of China(No.2021YFA1101100)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16020807)+3 种基金the Major Innovative Research Team of Suzhou,China(No.ZXT2019007)Suzhou Institute of Biomedical Engineering and Technology(SIBET)Jilin City Science and Technology Cooperation Project(No.E0550104)Science and Technology Innovation Talents in Universities of Henan Province and Doctor of Entrepreneurship and Innovation Program of Jiangsu Province in the year of 2020.
文摘The process of wound healing is routinely evaluated by histological evaluation in the clinic,which may cause scarring and secondary injury.Reflectance confocal microscopy(RCM)represents a noninvasive,real-time imaging technique that allows in vivo evaluation of the skin.Traditional RCM was wide-probe-based,which limited its application on uneven and covered skin.In this study,we report the development of a portable reflectance confocal microscope(PRCM)in which all components were assembled in a handheld shell.Although the size and weight of the PRCM were reduced based on the use of a microelectromechanical system,the resolution was kept at 0.91μm,and the field of view of the system was 343μm×532μm.When used in vivo,the PRCM was able to visualize cellular and nuclear morphology for both mouse and human skin.PRCM evaluations were then performed on wounds after topically applied mesenchymal stem cells(MSCs)or saline treatment.The PRCM allowed visualization of the formation of collagen bundles,re-epithelization from the wound edge to the wound bed,and hair follicle regeneration,which were consistent with histological evaluations.Therefore,we offer new insights into monitoring the effects of topically applied MSCs on the process of wound healing by using PRCM.This study illustrates that the newly developed PRCM represents a promising device for real-time,noninvasive monitoring of the dynamic process of wound healing,which demonstrates its potential to diagnose,monitor,or predict disease in clinical wound therapy.
基金This work was supported by the Natural Science Foundation of Hebei Province(Grant No.C2021201048)the Interdisciplinary Research Program of Natural Science of Hebei University(Grant No.513201422004)。
文摘Okra(Abelmoschus esculentus)is an important vegetable crop with high nutritional value.However,the mechanism underlying its high nutrient content remains poorly understood.Here,we present a chromosome-scale genome of okra with a size of 1.19 Gb.Comparative genomics analysis revealed the phylogenetic status of A.esculentus,as well as whole-genome duplication(WGD)events that have occurred widely across the Malvaceae species.We found that okra has experienced three additional WGDs compared with the diploid cotton Gossypium raimondii,resulting in a large chromosome number(2n=130).After three WGDs,okra has undergone extensive genomic deletions and retained substantial numbers of genes related to secondary metabolite biosynthesis and environmental adaptation,resulting in significant differences between okra and G.raimondii in the gene families related to cellulose synthesis.Combining transcriptomic and metabolomic analysis,we revealed the relationship between gene expression and metabolite content change across different okra developmental stages.Furthermore,the sinapic acid/S-lignin biosynthesis-related gene families have experienced remarkable expansion in okra,and the expression of key enzymes involved in the sinapic acid/S-lignin biosynthesis pathway vary greatly across developmental periods,which partially explains the differences in metabolite content across the different stages.Our study gains insights into the comprehensive evolutionary history of Malvaceae species and the genetic basis that underlies the nutrient content changes in okra,which will facilitate the functional study and genetic improvement of okra varieties.
基金supported by the National Natural Science Foundation of China (61972300, 61672401, 61373045, and 61902288,)the Pre-Research Project of the “Thirteenth Five-Year-Plan” of China (315***10101 and 315**0102)
文摘Personalized search utilizes user preferences to optimize search results,and most existing studies obtain user preferences by analyzing user behaviors in search engines that provide click-through data.However,the behavioral data are noisy because users often clicked some irrelevant documents to find their required information,and the new user cold start issue represents a serious problem,greatly reducing the performance of personalized search.This paper attempts to utilize online social network data to obtain user preferences that can be used to personalize search results,mine the knowledge of user interests,user influence and user relationships from online social networks,and use this knowledge to optimize the results returned by search engines.The proposed model is based on a holonic multiagent system that improves the adaptability and scalability of the model.The experimental results show that utilizing online social network data to implement personalized search is feasible and that online social network data are significant for personalized search.
基金the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16020807)the Key Research and Development Program of Jiangsu Province,China(Nos.BE2018668 and BE2017669)+2 种基金the Major Innovative Research Team of Suzhou,China(No.ZXT2019007)SIBET and Jilin City Science and Technology Cooperation Project(No.E0550104)Doctor of Entrepreneurship and Innovation Program of Jiangsu Province in the year of 2020.
文摘Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired.Recently,topical stem cell therapy has been realized as a promising strategy for wound healing and skin regeneration.However,stem cells must be administrated uniformly to the wound area,otherwise treatment will be ineffective,which has been a limitation of current administration methods.Specifically,the delivery pressure and nozzle features of most clinical cell spray devices are unknown,which may significantly affect the viability of sprayed cells and their capacity for proliferation.Herein,we developed a novel pneumatically assisted atomization device(PAAD)in which cell suspensions were uniformly atomized at a low delivery pressure.We optimized the applied fluidic pressure and air pressure to maximize cell survival and function for the application of multiple cell types,while ensuring uniform dispersal across the wound site.Moreover,we found that the application of sprayed umbilical cord-derived mesenchymal stem cells to wound sites significantly accelerated wound healing and promoted appendage regeneration on an excisional cutaneous wound model.Overall,the novel PAAD system delivered living cells uniformly and maintained the viability and differentiation of sprayed cells,strongly suggesting its potential for application in clinical cell therapy,especially for large,irregular,and severe skin wounds.
文摘User interest mining on Sina Weibo is the basis of personalized recommendations,advertising,marketing promotions,and other tasks.Although great progress has been made in this area,previous studies have ignored the differences among users:the varied behaviors and habits that lead to unique user data characteristics.It is unreasonable to use a single strategy to mine interests from such varied user data.Therefore,this paper proposes an adaptive model for user interest mining based on a multi-agent system whose input includes self-descriptive user data,microblogs and correlations.This method has the ability to select the appropriate strategy based on each user’s data characteristics.The experimental results show that the proposed method performs better than the baselines.
基金supported by the National Natural Science Foundation of China(32100500)Natural Science Foundation of Hebei Province(C2021201048)supported by the fund from the State Key Laboratory of Plant Genomics and the Chinese Academy of Sciences(CASWX2021SF-0109)。
文摘Common buckwheat(Fagopyrum esculentum)and Tartary buckwheat(Fagopyrum tataricum),the two most widely cultivated buckwheat species,differ greatly in flavonoid content and reproductive mode.Here,we report the first high-quality and chromosome-level genome assembly of common buckwheat with 1.2 Gb.Comparative genomic analysis revealed that common buckwheat underwent a burst of long terminal repeat retrotransposons insertion accompanied by numerous large chromosome rearrangements after divergence from Tartary buckwheat.Moreover,multiple gene families involved in stress tolerance and flavonoid biosynthesis such as multidrug and toxic compound extrusion(MATE)and chalcone synthase(CHS)underwent significant expansion in buckwheat,especially in common buckwheat.Integrated multi-omics analysis identified high expression of catechin biosynthesis-related genes in flower and seed in common buckwheat and high expression of rutin biosynthesis-related genes in seed in Tartary buckwheat as being important for the differences in flavonoid type and content between these buckwheat species.We also identified a candidate key rutindegrading enzyme gene(Ft8.2377)that was highly expressed in Tartary buckwheat seed.In addition,we identified a haplotype-resolved candidate locus containing many genes reportedly associated with the development of flower and pollen,which was potentially related to self-incompatibility in common buckwheat.Our study provides important resources facilitating future functional genomics-related research of flavonoid biosynthesis and selfincompatibility in buckwheat.
