The short-term insemination technique has been widely applied, although it is not clear whether the mechanical desorption of cumulus cells can affect normal fertilization and birth outcomes. Therefore, the present stu...The short-term insemination technique has been widely applied, although it is not clear whether the mechanical desorption of cumulus cells can affect normal fertilization and birth outcomes. Therefore, the present study aimed to evaluate whether early cumulus cell removal could affect in vitro fertilization outcomes. Based on a history of infertility and semen analysis, 61 patients were identified, and the cumulus cells were mechanically removed at either6 h post-insemination (Group A, 387 mature oocytes) or 18 - 20 h post-insemination as traditional in vitro fertilization (Group B, 424 mature oocytes). There were no significant differences in the rates of fertilization, available embryos, high quality embryos, birth, premature birth, pregnancy, or implantation when we compared the two groups. However, Group A exhibited a significantly higher polyspermy rate, and significantly lower rates of blastocyst formation and high-quality blastocyst formation, compared with Group B. Therefore, the results of present study indicate that early cumulus cell removal (at 6 h post-insemination) may negatively affect the rates of polyspermy, blastocyst formation, and high-quality blastocyst formation.展开更多
Crisp Nile tilapia(Oreochromis niloticus)is a kind of valuable fish product with high muscle firmness and crispiness texture.However,with the change of physicochemical in crisp Nile tilapia,the frozen storage paramete...Crisp Nile tilapia(Oreochromis niloticus)is a kind of valuable fish product with high muscle firmness and crispiness texture.However,with the change of physicochemical in crisp Nile tilapia,the frozen storage parameters and quality would be different comparing to normal Nile tilapia.Thus,the aim of this study was to analyze the changes in texture,chemical quality indices and volatile compounds of Nile tilapia fillets during frozen storage.The remaining storage time of the crisp Nile tilapia fillets could be estimated within 120 days.During frozen storage,fillets resulted in softer started at 90-day,and 36.75%,45.74%,48.81%and 20.37%reduction of hardness,springiness,gumminess and chewiness were observed at 120-day.Thiobarbituric acid reactive substances(TBARS)for frozen samples showed similar with fresh fillets within 60 days,while the TBARS was 1.97 folds higher than fresh one at 120-day.Low-field nuclear magnetic resonance(LF-NMR)indicated that the water loss of Nile tilapia fillets was significant changed at 120-day,which reduced more than 12.5%water out of weight.The volatile compound analysis showed that more free fatty acid will be detected at 120-day comparing to the fresh fillets.The combined results demonstrated that the crisp Nile tilapia fillets had the best quality before 60 days frozen storage then loss of some quality properties in longer freezing.Thus,these results identified the ideal storage strategy for the preservation of crisp Nile tilapia without affecting sensory appeal and commercial value.展开更多
Abstract Charcot-Marie-Tooth disease type 4B2 with early-onset glaucoma （CMT4B2, OMIM 604563） is a genetically-heterogeneous childhood-onset neuromuscular disorder. Here, we report the case of a 15-year-old male ado...Abstract Charcot-Marie-Tooth disease type 4B2 with early-onset glaucoma （CMT4B2, OMIM 604563） is a genetically-heterogeneous childhood-onset neuromuscular disorder. Here, we report the case of a 15-year-old male adolescent with lower extremity weakness, gait abnormalities, foot deformities and early-onset glaucoma. Since clinical diagnosis alone was insufficient for providing pathogenetic evidence to indicate that the condition belonged to a consanguineous family, we applied whole-exome sequencing to samples from the patient, his parents and his younger brother, assuming that the patient＇s condition is transmitted in an autosomal recessive pattern. A frame-shift mutation, c.4571delG （P.Gly1524Glufs＊42）, was revealed in the CMT4B2-related gene SBF2 （also known as MTMR13, MIM 607697）, and this mutation was found to be homozygous in the proband and heterozygous in his parents and younger brother. Together with the results of clinical diagnosis, this case was diagnosed as CMT4B2. Our finding further demonstrates the use of whole-exome sequencing in the diagnosis and treatment of rare diseases.展开更多
文摘The short-term insemination technique has been widely applied, although it is not clear whether the mechanical desorption of cumulus cells can affect normal fertilization and birth outcomes. Therefore, the present study aimed to evaluate whether early cumulus cell removal could affect in vitro fertilization outcomes. Based on a history of infertility and semen analysis, 61 patients were identified, and the cumulus cells were mechanically removed at either6 h post-insemination (Group A, 387 mature oocytes) or 18 - 20 h post-insemination as traditional in vitro fertilization (Group B, 424 mature oocytes). There were no significant differences in the rates of fertilization, available embryos, high quality embryos, birth, premature birth, pregnancy, or implantation when we compared the two groups. However, Group A exhibited a significantly higher polyspermy rate, and significantly lower rates of blastocyst formation and high-quality blastocyst formation, compared with Group B. Therefore, the results of present study indicate that early cumulus cell removal (at 6 h post-insemination) may negatively affect the rates of polyspermy, blastocyst formation, and high-quality blastocyst formation.
基金Key Research and Development Program of Guangzhou City(202103000067)Public science brand and standards establishment for Huadu Fishery Industrial Park(21302156)+1 种基金National Natural Science Foundation of China(42106130)Guangdong Basic and Applied Basic Research Foundation(2020A1515110237).
文摘Crisp Nile tilapia(Oreochromis niloticus)is a kind of valuable fish product with high muscle firmness and crispiness texture.However,with the change of physicochemical in crisp Nile tilapia,the frozen storage parameters and quality would be different comparing to normal Nile tilapia.Thus,the aim of this study was to analyze the changes in texture,chemical quality indices and volatile compounds of Nile tilapia fillets during frozen storage.The remaining storage time of the crisp Nile tilapia fillets could be estimated within 120 days.During frozen storage,fillets resulted in softer started at 90-day,and 36.75%,45.74%,48.81%and 20.37%reduction of hardness,springiness,gumminess and chewiness were observed at 120-day.Thiobarbituric acid reactive substances(TBARS)for frozen samples showed similar with fresh fillets within 60 days,while the TBARS was 1.97 folds higher than fresh one at 120-day.Low-field nuclear magnetic resonance(LF-NMR)indicated that the water loss of Nile tilapia fillets was significant changed at 120-day,which reduced more than 12.5%water out of weight.The volatile compound analysis showed that more free fatty acid will be detected at 120-day comparing to the fresh fillets.The combined results demonstrated that the crisp Nile tilapia fillets had the best quality before 60 days frozen storage then loss of some quality properties in longer freezing.Thus,these results identified the ideal storage strategy for the preservation of crisp Nile tilapia without affecting sensory appeal and commercial value.
基金supported by the National Natural Science Foundation of China (Grant No. 81172681)
文摘Abstract Charcot-Marie-Tooth disease type 4B2 with early-onset glaucoma （CMT4B2, OMIM 604563） is a genetically-heterogeneous childhood-onset neuromuscular disorder. Here, we report the case of a 15-year-old male adolescent with lower extremity weakness, gait abnormalities, foot deformities and early-onset glaucoma. Since clinical diagnosis alone was insufficient for providing pathogenetic evidence to indicate that the condition belonged to a consanguineous family, we applied whole-exome sequencing to samples from the patient, his parents and his younger brother, assuming that the patient＇s condition is transmitted in an autosomal recessive pattern. A frame-shift mutation, c.4571delG （P.Gly1524Glufs＊42）, was revealed in the CMT4B2-related gene SBF2 （also known as MTMR13, MIM 607697）, and this mutation was found to be homozygous in the proband and heterozygous in his parents and younger brother. Together with the results of clinical diagnosis, this case was diagnosed as CMT4B2. Our finding further demonstrates the use of whole-exome sequencing in the diagnosis and treatment of rare diseases.
