Bacterial or viral infections,such as Brucella,mumps virus,herpes simplex virus,and Zika virus,destroy immune homeostasis of the testes,leading to spermatogenesis disorder and infertility.Of note,recent research shows...Bacterial or viral infections,such as Brucella,mumps virus,herpes simplex virus,and Zika virus,destroy immune homeostasis of the testes,leading to spermatogenesis disorder and infertility.Of note,recent research shows that SARS-CoV-2 can infect male gonads and destroy Sertoli and Leydig cells,leading to male reproductive dysfunction.Due to the many side effects associated with antibiotic therapy,finding alternative treatments for inflammatory injury remains critical.Here,we found that Dmrt1 plays an important role in regulating testicular immune homeostasis.Knockdown of Dmrt1 in male mice inhibited spermatogenesis with a broad inflammatory response in seminiferous tubules and led to the loss of spermatogenic epithelial cells.Chromatin immunoprecipitation sequencing(ChIP-seq)and RNA sequencing(RNA-seq)revealed that Dmrt1 positively regulated the expression of Spry1,an inhibitory protein of the receptor tyrosine kinase(RTK)signaling pathway.Furthermore,immunoprecipitation-mass spectrometry(IP-MS)and co-immunoprecipitation(Co-IP)analysis indicated that SPRY1 binds to nuclear factor kappa B1(NF-κB1)to prevent nuclear translocation of p65,inhibit activation of NF-κB signaling,prevent excessive inflammatory reaction in the testis,and protect the integrity of the blood-testis barrier.In view of this newly identified Dmrt1-Spry1-NF-κB axis mechanism in the regulation of testicular immune homeostasis,our study opens new avenues for the prevention and treatment of male reproductive diseases in humans and livestock.展开更多
Single-cell RNA sequencing(scRNA-seq)is useful for exploring cell heterogeneity.For large animals,however,little is known regarding spermatogonial stem cell(SSC)selfrenewal regulation,especially in dairy goats.In this...Single-cell RNA sequencing(scRNA-seq)is useful for exploring cell heterogeneity.For large animals,however,little is known regarding spermatogonial stem cell(SSC)selfrenewal regulation,especially in dairy goats.In this study,we described a high-resolution scRNA-seq atlas derived from a dairy goat.We identified six somatic cell and five spermatogenic cell subtypes.During spermatogenesis,genes with significantly changed expression were mainly enriched in the Notch,TGF-β,and Hippo signaling pathways as well as the signaling pathway involved in the regulation of stem cell pluripotency.We detected and screened specific candidate marker genes(TKTL1 and AES)for spermatogonia.Our study provides new insights into goat spermatogenesis and the development of testicular somatic cells.展开更多
Double sex and mab-3-related transcription factor 1(Dmrt1),which is expressed in goat male germline stem cells(mGSCs)and Sertoli cells,is one of the most conserved transcription factors involved in sex determination.I...Double sex and mab-3-related transcription factor 1(Dmrt1),which is expressed in goat male germline stem cells(mGSCs)and Sertoli cells,is one of the most conserved transcription factors involved in sex determination.In this study,we highlighted the role of Dmrt1 in balancing the innate immune response in goat mGSCs.Dmrt1 recruited promyelocytic leukemia zinc finger(Plzf),also known as zinc finger and BTB domain-containing protein 16(Zbtb16),to repress the Toll-like receptor 4(TLR4)-dependent inflammatory signaling pathway and nuclear factor(NF)-κB.Knockdown of Dmrt1 in seminiferous tubules resulted in widespread degeneration of germ and somatic cells,while the expression of proinflammatory factors were significantly enhanced.We also demonstrated that Dmrt1 stimulated proliferation of mGSCs,but repressed apoptosis caused by the immune response.Thus,Dmrt1 is sufficient to reduce inflammation in the testes,thereby establishing the stability of spermatogenesis and the testicular microenvironment.展开更多
Targeting the problem of high real-time requirements in astronomical data processing,this paper proposes a real-time early warning model for light curves based on a Gated Recurrent Unit(GRU)network.Using the memory fu...Targeting the problem of high real-time requirements in astronomical data processing,this paper proposes a real-time early warning model for light curves based on a Gated Recurrent Unit(GRU)network.Using the memory function of the GRU network,a prediction model of the light curve is established,and the model is trained using the collected light curve data,so that the model can predict a star magnitude value for the next moment based on historical star magnitude data.In this paper,we calculate the difference between the model prediction value and the actual observation value and set a threshold.If the difference exceeds the set threshold,the observation value at the next moment is considered to be an abnormal value,and a warning is given.Astronomers can carry out further certification based on the early warning and in combination with other means of observation.The method proposed in this paper can be applied to real-time observations in time domain astronomy.展开更多
We measure the distance to the supernova remnant G15.4+0.1 which is likely associated with Te V source HESS J1818–154. We build the neutral hydrogen(HI) absorption and ^(13)CO spectra for supernova remnant G15.4+0.1 ...We measure the distance to the supernova remnant G15.4+0.1 which is likely associated with Te V source HESS J1818–154. We build the neutral hydrogen(HI) absorption and ^(13)CO spectra for supernova remnant G15.4+0.1 by employing data from the Southern Galactic Plane Survey(SGPS) and the HI/OH/Recombination line survey(THOR). The maximum absorption velocity of about 140 km s^(-1) constrains the lower limit of its distance to about 8.0 kpc. Further, the fact that the HI emission feature at about 95 km s^(-1) seems to have no corresponding absorption suggests that G15.4+0.1 likely has an upper limit for distance of about 10.5 kpc. The ^(13)CO spectrum for the remnant supports our measurement. The new distance provides revised parameters on its associated pulsar wind nebula and Te V source.展开更多
Detecting supernova remnant(SNR) candidates in the interstellar medium is a challenging task because SNRs have weak radio signals and irregular shapes. The use of a convolutional neural network is a deep learning meth...Detecting supernova remnant(SNR) candidates in the interstellar medium is a challenging task because SNRs have weak radio signals and irregular shapes. The use of a convolutional neural network is a deep learning method that can help us extract various features from images. To extract SNRs from astronomical images and estimate the positions of SNR candidates, we design the SNR-Net model composed of a training component and a detection component. In addition, transfer learning is used to initialize the network parameters, which improves the speed and accuracy of network training. We apply a T-T plot(of the different brightness temperatures of map pixels at two different frequencies) to calculate the spectral index of SNR candidates. To accelerate the scientific computing process, we take advantage of innovative hardware architecture, such as deep learning optimized graphics processing units, which increases the speed of computation by a factor of 5. A case study suggests that SNR-Net may be applicable to detecting extended sources in the images automatically.展开更多
We simulate the evolution of supernova remnants(SNRs) in a strong magnetic field. Usually,supernovae explode in a normal interstellar medium with magnetic field of no more than 50 μG, which has been well studied. How...We simulate the evolution of supernova remnants(SNRs) in a strong magnetic field. Usually,supernovae explode in a normal interstellar medium with magnetic field of no more than 50 μG, which has been well studied. However, the surrounding magnetic field will be much stronger in some situations, such as in a galactic center. Therefore, we try to explore these situations. The simulations show that a strong magnetic field of 1 mG will align the motion of ejecta in a way similar to a jet. The ejecta propagating perpendicularly to the magnetic field will be reflected and generate a strong reverse shock. When the reverse shock converges in the explosion center, it will more or less flow along the central magnetic field. Finally,most of the ejecta will propagate parallel to the magnetic field.展开更多
We take advantage of red clump stars to build the relation of the optical extinction(AV) and distance in each direction of supernova remnants(SNRs) with known extinction in the fourth Galactic quadrant.The distances o...We take advantage of red clump stars to build the relation of the optical extinction(AV) and distance in each direction of supernova remnants(SNRs) with known extinction in the fourth Galactic quadrant.The distances of nine SNRs are determined well by this method.Their uncertainties range from 10% to30%,which are significantly improved for eight SNRs,G279.0+1.1,G284.3–1.8,G296.1–0.5,G299.2–2.9,G308.4–1.4,G309.2–0.6,G309.8–2.6 and G332.4–0.4.In addition,SNR G284.3–1.8 with the new distance of 5.5 kpc is not likely associated with the PSR J1016–5857 at 3 kpc.展开更多
基金supported by the National Natural Science Foundation of China(32072806,31572399)National Key Research and Development Program of China(2022YFD1302201)+3 种基金Program of Shaanxi Province Science and Technology Innovation Team(2019TD-036)Major Projects of Natural Science Foundation of Inner Mongolia Autonomous Region(2020ZD10)Key Research and Development Program of Shaanxi Province(2022NY-044)Key Technologies Demonstration of Animal Husbandry in Shaanxi Province(20221086)。
文摘Bacterial or viral infections,such as Brucella,mumps virus,herpes simplex virus,and Zika virus,destroy immune homeostasis of the testes,leading to spermatogenesis disorder and infertility.Of note,recent research shows that SARS-CoV-2 can infect male gonads and destroy Sertoli and Leydig cells,leading to male reproductive dysfunction.Due to the many side effects associated with antibiotic therapy,finding alternative treatments for inflammatory injury remains critical.Here,we found that Dmrt1 plays an important role in regulating testicular immune homeostasis.Knockdown of Dmrt1 in male mice inhibited spermatogenesis with a broad inflammatory response in seminiferous tubules and led to the loss of spermatogenic epithelial cells.Chromatin immunoprecipitation sequencing(ChIP-seq)and RNA sequencing(RNA-seq)revealed that Dmrt1 positively regulated the expression of Spry1,an inhibitory protein of the receptor tyrosine kinase(RTK)signaling pathway.Furthermore,immunoprecipitation-mass spectrometry(IP-MS)and co-immunoprecipitation(Co-IP)analysis indicated that SPRY1 binds to nuclear factor kappa B1(NF-κB1)to prevent nuclear translocation of p65,inhibit activation of NF-κB signaling,prevent excessive inflammatory reaction in the testis,and protect the integrity of the blood-testis barrier.In view of this newly identified Dmrt1-Spry1-NF-κB axis mechanism in the regulation of testicular immune homeostasis,our study opens new avenues for the prevention and treatment of male reproductive diseases in humans and livestock.
基金supported by the National Natural Science Foundation of China(31572399,32072806,32072815,32002246,61772431,62072377)Program of Shaanxi Province Science and Technology Innovation Team(2019TD-036)+4 种基金Program of State Key Lab of Reproductive Regulation&Breeding of Grassland Livestock(SKL-OT-201801)Program of State Key Laboratory of Respiratory Disease(SKLRD-OP-202114)Science and Technology Major Project of Inner Mongolia Autonomous Region of China(ZDZX2018065)the First-class University and Academic Program from Northwest A&F University(Z1010221003)Financial aid for basic operation fee of Central University(Z1090219146)。
文摘Single-cell RNA sequencing(scRNA-seq)is useful for exploring cell heterogeneity.For large animals,however,little is known regarding spermatogonial stem cell(SSC)selfrenewal regulation,especially in dairy goats.In this study,we described a high-resolution scRNA-seq atlas derived from a dairy goat.We identified six somatic cell and five spermatogenic cell subtypes.During spermatogenesis,genes with significantly changed expression were mainly enriched in the Notch,TGF-β,and Hippo signaling pathways as well as the signaling pathway involved in the regulation of stem cell pluripotency.We detected and screened specific candidate marker genes(TKTL1 and AES)for spermatogonia.Our study provides new insights into goat spermatogenesis and the development of testicular somatic cells.
基金This work was supported by the China National Basic Research Program(2016YFA0100203)National Natural Science Foundation of China(31572399Detail,32072806,32072815,32002246)+3 种基金State Key Lab of Reproductive Regulation&Breeding of Grassland Livestock(SKL-OT-201801)Science and Technology Major Project of Inner Mongolia Autonomous Region of China(ZDZX2018065)and Shaanxi Province Science and Technology Innovation Team(2019TD-036)The authors thank Dr.John Clotaire Daguia Zambe for helpful comments about this paper,Jia Fang for the PGL3-NF-κB luciferase reporter plasmid,and Dong-Xue Che for bioinformatics analysis.
文摘Double sex and mab-3-related transcription factor 1(Dmrt1),which is expressed in goat male germline stem cells(mGSCs)and Sertoli cells,is one of the most conserved transcription factors involved in sex determination.In this study,we highlighted the role of Dmrt1 in balancing the innate immune response in goat mGSCs.Dmrt1 recruited promyelocytic leukemia zinc finger(Plzf),also known as zinc finger and BTB domain-containing protein 16(Zbtb16),to repress the Toll-like receptor 4(TLR4)-dependent inflammatory signaling pathway and nuclear factor(NF)-κB.Knockdown of Dmrt1 in seminiferous tubules resulted in widespread degeneration of germ and somatic cells,while the expression of proinflammatory factors were significantly enhanced.We also demonstrated that Dmrt1 stimulated proliferation of mGSCs,but repressed apoptosis caused by the immune response.Thus,Dmrt1 is sufficient to reduce inflammation in the testes,thereby establishing the stability of spermatogenesis and the testicular microenvironment.
基金supported by the National Natural Science Foundation of China(No.41672323)the Interdiscipline Research Funds of Beijing Normal University
文摘Targeting the problem of high real-time requirements in astronomical data processing,this paper proposes a real-time early warning model for light curves based on a Gated Recurrent Unit(GRU)network.Using the memory function of the GRU network,a prediction model of the light curve is established,and the model is trained using the collected light curve data,so that the model can predict a star magnitude value for the next moment based on historical star magnitude data.In this paper,we calculate the difference between the model prediction value and the actual observation value and set a threshold.If the difference exceeds the set threshold,the observation value at the next moment is considered to be an abnormal value,and a warning is given.Astronomers can carry out further certification based on the early warning and in combination with other means of observation.The method proposed in this paper can be applied to real-time observations in time domain astronomy.
基金support from the National Natural Science Foundation of China (11473038 and 11273025)
文摘We measure the distance to the supernova remnant G15.4+0.1 which is likely associated with Te V source HESS J1818–154. We build the neutral hydrogen(HI) absorption and ^(13)CO spectra for supernova remnant G15.4+0.1 by employing data from the Southern Galactic Plane Survey(SGPS) and the HI/OH/Recombination line survey(THOR). The maximum absorption velocity of about 140 km s^(-1) constrains the lower limit of its distance to about 8.0 kpc. Further, the fact that the HI emission feature at about 95 km s^(-1) seems to have no corresponding absorption suggests that G15.4+0.1 likely has an upper limit for distance of about 10.5 kpc. The ^(13)CO spectrum for the remnant supports our measurement. The new distance provides revised parameters on its associated pulsar wind nebula and Te V source.
基金supported bythe National Natural Science Foundation of China(No. 41272359)the Ministry of Land and Resourcesfor the Public Welfare Industry Research Projects(201511079-02)the Natural Science Foundation ofShandong (No. ZR2015FL006)
文摘Detecting supernova remnant(SNR) candidates in the interstellar medium is a challenging task because SNRs have weak radio signals and irregular shapes. The use of a convolutional neural network is a deep learning method that can help us extract various features from images. To extract SNRs from astronomical images and estimate the positions of SNR candidates, we design the SNR-Net model composed of a training component and a detection component. In addition, transfer learning is used to initialize the network parameters, which improves the speed and accuracy of network training. We apply a T-T plot(of the different brightness temperatures of map pixels at two different frequencies) to calculate the spectral index of SNR candidates. To accelerate the scientific computing process, we take advantage of innovative hardware architecture, such as deep learning optimized graphics processing units, which increases the speed of computation by a factor of 5. A case study suggests that SNR-Net may be applicable to detecting extended sources in the images automatically.
基金supported by the National Key R&D Program of China (2018YFA0404203)
文摘We simulate the evolution of supernova remnants(SNRs) in a strong magnetic field. Usually,supernovae explode in a normal interstellar medium with magnetic field of no more than 50 μG, which has been well studied. However, the surrounding magnetic field will be much stronger in some situations, such as in a galactic center. Therefore, we try to explore these situations. The simulations show that a strong magnetic field of 1 mG will align the motion of ejecta in a way similar to a jet. The ejecta propagating perpendicularly to the magnetic field will be reflected and generate a strong reverse shock. When the reverse shock converges in the explosion center, it will more or less flow along the central magnetic field. Finally,most of the ejecta will propagate parallel to the magnetic field.
基金supports from the National Key R&D Programs of China (2018YFA0404203)the National Natural Science Foundation of China (Grant Nos. 11603039 and U1831128)
文摘We take advantage of red clump stars to build the relation of the optical extinction(AV) and distance in each direction of supernova remnants(SNRs) with known extinction in the fourth Galactic quadrant.The distances of nine SNRs are determined well by this method.Their uncertainties range from 10% to30%,which are significantly improved for eight SNRs,G279.0+1.1,G284.3–1.8,G296.1–0.5,G299.2–2.9,G308.4–1.4,G309.2–0.6,G309.8–2.6 and G332.4–0.4.In addition,SNR G284.3–1.8 with the new distance of 5.5 kpc is not likely associated with the PSR J1016–5857 at 3 kpc.
