Chinese wingnut(Pterocarya stenoptera)is a medicinally and economically important tree species within the family Juglandaceae.However,the lack of high-quality reference genome has hindered its in-depth research.In thi...Chinese wingnut(Pterocarya stenoptera)is a medicinally and economically important tree species within the family Juglandaceae.However,the lack of high-quality reference genome has hindered its in-depth research.In this study,we successfully assembled its chromosome-level genome and performed multiomics analyses to address its evolutionary history and synthesis of medicinal components.A thorough examination of genomes has uncovered a significant expansion in the Lateral Organ Boundaries Domain gene family among the winged group in Juglandaceae.This notable increase may be attributed to their frequent exposure to flood-prone environments.After further differentiation between Chinese wingnut and Cyclocarya paliurus,significant positive selection occurred on the genes of NADH dehydrogenase related to mitochondrial aerobic respiration in Chinese wingnut,enhancing its ability to cope with waterlogging stress.Comparative genomic analysis revealed Chinese wingnut evolved more unique genes related to arginine synthesis,potentially endowing it with a higher capacity to purify nutrient-rich water bodies.Expansion of terpene synthase families enables the production of increased quantities of terpenoid volatiles,potentially serving as an evolved defense mechanism against herbivorous insects.Through combined transcriptomic and metabolomic analysis,we identified the candidate genes involved in the synthesis of terpenoid volatiles.Our study offers essential genetic resources for Chinese wingnut,unveiling its evolutionary history and identifying key genes linked to the production of terpenoid volatiles.展开更多
Polynaphthalimide(PNI)with six-membered imide ring(_(6-)PI)has better chemical resistance than five-membered imide ring(_(5-)PI),but is difficult to be processed into nano fibers due to the poor processability.In this...Polynaphthalimide(PNI)with six-membered imide ring(_(6-)PI)has better chemical resistance than five-membered imide ring(_(5-)PI),but is difficult to be processed into nano fibers due to the poor processability.In this work,we proposed a template strategy to fabricate nanofiber _(6-)PI membranes and their composite membranes.Neat _(6-)PI and _(6-)PI composite fibrous membranes were prepared using high-molecular-weight polymers _(5-)PAA and PVP as templates by electrospinning.FTIR,DMA,TGA and tensile tests were used to characterize their chemical structures,thermal stability and mechanical properties.Further eye-observation,micro-morphology investigation and tensile tests were applied to evaluate the chemical resistance of nanofibrous membranes in strong acid,strong alkaline,and concentrated salt.The results demonstrated that 6-PI nano fibrous membra nes possessed the best thermal stability,best acid,alkaline,and salt resista nee with the highest mecha nical rete ntion.This study will provide basic information for high-performance electrospun 6-PI nanofiber membranes and provide opportunities for applications of Pls in different chemically harsh environments.展开更多
基金supported by National Natural Science Foundation of China(32360307)the Natural Science Foundation of Inner Mongolia(2023MS03031)+1 种基金Inner Mongolia Grassland Talents Project(3211002406)the Open Fund of State Key Laboratory of Tree Genetics and Breeding(Chinese Academy of Forestry)(Grant No.TGB2021004).
文摘Chinese wingnut(Pterocarya stenoptera)is a medicinally and economically important tree species within the family Juglandaceae.However,the lack of high-quality reference genome has hindered its in-depth research.In this study,we successfully assembled its chromosome-level genome and performed multiomics analyses to address its evolutionary history and synthesis of medicinal components.A thorough examination of genomes has uncovered a significant expansion in the Lateral Organ Boundaries Domain gene family among the winged group in Juglandaceae.This notable increase may be attributed to their frequent exposure to flood-prone environments.After further differentiation between Chinese wingnut and Cyclocarya paliurus,significant positive selection occurred on the genes of NADH dehydrogenase related to mitochondrial aerobic respiration in Chinese wingnut,enhancing its ability to cope with waterlogging stress.Comparative genomic analysis revealed Chinese wingnut evolved more unique genes related to arginine synthesis,potentially endowing it with a higher capacity to purify nutrient-rich water bodies.Expansion of terpene synthase families enables the production of increased quantities of terpenoid volatiles,potentially serving as an evolved defense mechanism against herbivorous insects.Through combined transcriptomic and metabolomic analysis,we identified the candidate genes involved in the synthesis of terpenoid volatiles.Our study offers essential genetic resources for Chinese wingnut,unveiling its evolutionary history and identifying key genes linked to the production of terpenoid volatiles.
基金by the National Natural Science Foundation of China(Nos.21975111,21774053,and 51803093)Natural Science Foundation of Jiangsu Province(No.BK20180770)and Open Project of Chemistry Department of Qin gdao Un iversity of Scie nee and Technology(No.QUSTHX201921).
文摘Polynaphthalimide(PNI)with six-membered imide ring(_(6-)PI)has better chemical resistance than five-membered imide ring(_(5-)PI),but is difficult to be processed into nano fibers due to the poor processability.In this work,we proposed a template strategy to fabricate nanofiber _(6-)PI membranes and their composite membranes.Neat _(6-)PI and _(6-)PI composite fibrous membranes were prepared using high-molecular-weight polymers _(5-)PAA and PVP as templates by electrospinning.FTIR,DMA,TGA and tensile tests were used to characterize their chemical structures,thermal stability and mechanical properties.Further eye-observation,micro-morphology investigation and tensile tests were applied to evaluate the chemical resistance of nanofibrous membranes in strong acid,strong alkaline,and concentrated salt.The results demonstrated that 6-PI nano fibrous membra nes possessed the best thermal stability,best acid,alkaline,and salt resista nee with the highest mecha nical rete ntion.This study will provide basic information for high-performance electrospun 6-PI nanofiber membranes and provide opportunities for applications of Pls in different chemically harsh environments.
