While the rechargeable aqueous zinc-ion batteries(AZIBs)have been recognized as one of the most viable batteries for scale-up application,the instability on Zn anode–electrolyte interface bottleneck the further devel...While the rechargeable aqueous zinc-ion batteries(AZIBs)have been recognized as one of the most viable batteries for scale-up application,the instability on Zn anode–electrolyte interface bottleneck the further development dramatically.Herein,we utilize the amino acid glycine(Gly)as an electrolyte additive to stabilize the Zn anode–electrolyte interface.The unique interfacial chemistry is facilitated by the synergistic“anchor-capture”effect of polar groups in Gly molecule,manifested by simultaneously coupling the amino to anchor on the surface of Zn anode and the carboxyl to capture Zn^(2+)in the local region.As such,this robust anode–electrolyte interface inhibits the disordered migration of Zn^(2+),and effectively suppresses both side reactions and dendrite growth.The reversibility of Zn anode achieves a significant improvement with an average Coulombic efficiency of 99.22%at 1 mA cm^(−2)and 0.5 mAh cm^(−2)over 500 cycles.Even at a high Zn utilization rate(depth of discharge,DODZn)of 68%,a steady cycle life up to 200 h is obtained for ultrathin Zn foils(20μm).The superior rate capability and long-term cycle stability of Zn–MnO_(2)full cells further prove the effectiveness of Gly in stabilizing Zn anode.This work sheds light on additive designing from the specific roles of polar groups for AZIBs.展开更多
目的观察人脐带间充质干细胞(HUMSC)对糖尿病肾病大鼠肾脏缺氧诱导因子(HIF-1α)表达的影响。方法50只健康清洁级8周龄雄性SD大鼠,随机选取20只为健康对照组,其余30只采用链脲菌素复制糖尿病肾病大鼠模型(模型组),两组大鼠饲养12周。第1...目的观察人脐带间充质干细胞(HUMSC)对糖尿病肾病大鼠肾脏缺氧诱导因子(HIF-1α)表达的影响。方法50只健康清洁级8周龄雄性SD大鼠,随机选取20只为健康对照组,其余30只采用链脲菌素复制糖尿病肾病大鼠模型(模型组),两组大鼠饲养12周。第12周,随机选取两组大鼠各3只分别尾静脉注射DiR-HUMSCs,代谢12~16 h后在小动物活体光学3D成像系统下观察DiR-HUMSCs在大鼠体内的分布。随机选取9只模型组大鼠进行HUMSCs移植(HUMSCs移植组)。HUMSCs移植采用尾静脉注射方式移植浓度为1×10^(6)个/mL HUMSCs 500μL至大鼠体内,每周1次,连续4周,共28 d。检测3组大鼠的24 h尿蛋白定量(24 h UPro)、血清肌酐(Scr)、血尿素氮(BUN)、尿肌酐(Ucr)、尿白蛋白与肌酐比值(UACR)水平;酶联免疫吸附试验(ELISA)检测3组大鼠血清HIF-1α水平;采用PAS和Masson染色进行肾脏组织病理检测;免疫荧光法检测3组大鼠肾组织HIF-1α、Slc12A3和Aquaporin1蛋白的表达。结果移植HUMSCs治疗4周后,与健康对照组比较,模型组和HUMSCs移植组大鼠Ucr水平降低(P<0.05),Scr、24 h UPro、BUN、UCAR均升高(P<0.05);与模型组比较,HUMSCs移植组大鼠Ucr水平差异无统计学意义(P>0.05),Scr、24 h UPro、BUN、UCAR均降低(P<0.05)。糖尿病肾病大鼠病理损伤缓解,系膜增生和基底膜增厚改善,小管空泡变性减少,间质纤维化减轻。模型组大鼠血清HIF-1α水平较健康对照组升高(P<0.05),HUMSCs移植组血清HIF-1α水平较模型组下降(P<0.05)。与健康对照组比较,模型组大鼠肾脏远端小管中HIF-1α蛋白水平增加(P<0.05);HUMSCs移植组的HIF-1α蛋白水平较模型组降低(P<0.05)。模型组远端小管标记蛋白Slc12A3水平低于健康对照组(P<0.05),HUMSCs移植组Slc12A3水平较模型组升高(P<0.05)。HUMSCs移植组的Aquaporin1蛋白水平较模型组和健康对照组均降低(P<0.05)。糖尿病肾病大鼠近端小管中无HIF-1α表达。结论HIF-1α主要在糖尿病肾病大鼠肾脏远端小管表达,且HUMSCs可通过抑制HIF-1α的表达修复肾小管的损伤。展开更多
Finding energetic materials with tailored properties is always a significant challenge due to low research efficiency in trial and error.Herein,a methodology combining domain knowledge,a machine learning algorithm,and...Finding energetic materials with tailored properties is always a significant challenge due to low research efficiency in trial and error.Herein,a methodology combining domain knowledge,a machine learning algorithm,and experiments is presented for accelerating the discovery of novel energetic materials.A high-throughput virtual screening(HTVS)system integrating on-demand molecular generation and machine learning models covering the prediction of molecular properties and crystal packing mode scoring is established.With the proposed HTVS system,candidate molecules with promising properties and a desirable crystal packing mode are rapidly targeted from the generated molecular space containing 25112 molecules.Furthermore,a study of the crystal structure and properties shows that the good comprehensive performances of the target molecule are in agreement with the predicted results,thus verifying the effectiveness of the proposed methodology.This work demonstrates a new research paradigm for discovering novel energetic materials and can be extended to other organic materials without manifest obstacles.展开更多
A novel third-generation hydrogen peroxide（H2O2） biosensor（Hb/CdS/MWNTs/GCE） was fabricated through hemoglobin（Hb） adsorbed onto the mercaptoacetic acid modified CdS QDs/carboxyl multiwall carbon nanotubes＇（MW...A novel third-generation hydrogen peroxide（H2O2） biosensor（Hb/CdS/MWNTs/GCE） was fabricated through hemoglobin（Hb） adsorbed onto the mercaptoacetic acid modified CdS QDs/carboxyl multiwall carbon nanotubes＇（MWNTs） films. Cyclic voltammogram of Hb/CdS/MWNTs/GCE showed a pair of well-defined and quasi-reversible redox peaks with a formal potential（E^0） of-0.230 V（vs. Ag/AgCl） in 0.1 mol/L pH=8.0 phosphate buffer solution（PBS）, which was the characteristic of the Hb heme Fe（Ⅲ）/Fe（Ⅱ） redox couple. The biosensor shows an excellent electrocatalytic activity to the reduction of H2O2. The response time of the designed biosensor to H202 at a potential of-0.30 V was less than 2 s and linear relationships were obtained in the concentration ranges of 2.0×10^-6-2.7×10^-3 mol/L and 2.7×10^-3-7.7×10^-3 mol/L with a detection limit of 3.0×10^-7 mol/L（S/N=3）. The apparent Michaelis-Menten constant Km was estimated to be 1.324 mmol/L that illustrated the excellent biological activity of the fixed Hb.展开更多
OBJECTIVE Learning and memory impairment is one of the common sequelae of stroke patients,which is called"post-stroke dementia"and seriously affects the quality of life of the patients.For post-stroke dement...OBJECTIVE Learning and memory impairment is one of the common sequelae of stroke patients,which is called"post-stroke dementia"and seriously affects the quality of life of the patients.For post-stroke dementia,there is still no effective clinical treatment.In the present study,we aim to investigate the effect of the active ingredient of Ferula sinkiangensis,AW09,on global cerebral ischemia-reperfusion mice.METHODS The bilateral common carotid artery occlusion(BCCAO)reperfusion model was used to investigate the protective effect of AW09 on cognitive dysfunction in mice with global cerebral ischemia reperfusion.Y-maze and Morris water maze were used to test the learning and memory ability of mice.RESULTS Y-maze test showed that AW09 treatment significantly increased the spontaneous alternation rate of BCCAO model animals and had no significant effect on the total number of arm entries.The results of Morris water maze showed that AW09 significantly reduced the escape latency of BCCAO mice during the training period.During the probe test phase,AW09 significantly increased the swimming time in target quadrant,distance in target quadrant and number of platform crossings and decreased the swimming time in the quadrant opposite the target quadrant of BCCAO mice.CONCLUSION AW09,the active ingredient of Ferula sinkiangensis,can improve working memory impairment and spatial memory impairment in animals with global cerebral ischemia-reperfusion,suggesting that AW09 has poten⁃tial therapeutic value for cognitive dysfunction caused by global cerebral ischemia.展开更多
基金supported by National Key R&D Program(2022YFB2502000)Zhejiang Provincial Natural Science Foundation of China(LZ23B030003)+1 种基金the Fundamental Research Funds for the Central Universities(2021FZZX001-09)the National Natural Science Foundation of China(52175551).
文摘While the rechargeable aqueous zinc-ion batteries(AZIBs)have been recognized as one of the most viable batteries for scale-up application,the instability on Zn anode–electrolyte interface bottleneck the further development dramatically.Herein,we utilize the amino acid glycine(Gly)as an electrolyte additive to stabilize the Zn anode–electrolyte interface.The unique interfacial chemistry is facilitated by the synergistic“anchor-capture”effect of polar groups in Gly molecule,manifested by simultaneously coupling the amino to anchor on the surface of Zn anode and the carboxyl to capture Zn^(2+)in the local region.As such,this robust anode–electrolyte interface inhibits the disordered migration of Zn^(2+),and effectively suppresses both side reactions and dendrite growth.The reversibility of Zn anode achieves a significant improvement with an average Coulombic efficiency of 99.22%at 1 mA cm^(−2)and 0.5 mAh cm^(−2)over 500 cycles.Even at a high Zn utilization rate(depth of discharge,DODZn)of 68%,a steady cycle life up to 200 h is obtained for ultrathin Zn foils(20μm).The superior rate capability and long-term cycle stability of Zn–MnO_(2)full cells further prove the effectiveness of Gly in stabilizing Zn anode.This work sheds light on additive designing from the specific roles of polar groups for AZIBs.
文摘目的观察人脐带间充质干细胞(HUMSC)对糖尿病肾病大鼠肾脏缺氧诱导因子(HIF-1α)表达的影响。方法50只健康清洁级8周龄雄性SD大鼠,随机选取20只为健康对照组,其余30只采用链脲菌素复制糖尿病肾病大鼠模型(模型组),两组大鼠饲养12周。第12周,随机选取两组大鼠各3只分别尾静脉注射DiR-HUMSCs,代谢12~16 h后在小动物活体光学3D成像系统下观察DiR-HUMSCs在大鼠体内的分布。随机选取9只模型组大鼠进行HUMSCs移植(HUMSCs移植组)。HUMSCs移植采用尾静脉注射方式移植浓度为1×10^(6)个/mL HUMSCs 500μL至大鼠体内,每周1次,连续4周,共28 d。检测3组大鼠的24 h尿蛋白定量(24 h UPro)、血清肌酐(Scr)、血尿素氮(BUN)、尿肌酐(Ucr)、尿白蛋白与肌酐比值(UACR)水平;酶联免疫吸附试验(ELISA)检测3组大鼠血清HIF-1α水平;采用PAS和Masson染色进行肾脏组织病理检测;免疫荧光法检测3组大鼠肾组织HIF-1α、Slc12A3和Aquaporin1蛋白的表达。结果移植HUMSCs治疗4周后,与健康对照组比较,模型组和HUMSCs移植组大鼠Ucr水平降低(P<0.05),Scr、24 h UPro、BUN、UCAR均升高(P<0.05);与模型组比较,HUMSCs移植组大鼠Ucr水平差异无统计学意义(P>0.05),Scr、24 h UPro、BUN、UCAR均降低(P<0.05)。糖尿病肾病大鼠病理损伤缓解,系膜增生和基底膜增厚改善,小管空泡变性减少,间质纤维化减轻。模型组大鼠血清HIF-1α水平较健康对照组升高(P<0.05),HUMSCs移植组血清HIF-1α水平较模型组下降(P<0.05)。与健康对照组比较,模型组大鼠肾脏远端小管中HIF-1α蛋白水平增加(P<0.05);HUMSCs移植组的HIF-1α蛋白水平较模型组降低(P<0.05)。模型组远端小管标记蛋白Slc12A3水平低于健康对照组(P<0.05),HUMSCs移植组Slc12A3水平较模型组升高(P<0.05)。HUMSCs移植组的Aquaporin1蛋白水平较模型组和健康对照组均降低(P<0.05)。糖尿病肾病大鼠近端小管中无HIF-1α表达。结论HIF-1α主要在糖尿病肾病大鼠肾脏远端小管表达,且HUMSCs可通过抑制HIF-1α的表达修复肾小管的损伤。
基金the Science Challenge Project(TZ2018004)the National Natural Science Foundation of China(21875228 and 21702195)for financial support。
文摘Finding energetic materials with tailored properties is always a significant challenge due to low research efficiency in trial and error.Herein,a methodology combining domain knowledge,a machine learning algorithm,and experiments is presented for accelerating the discovery of novel energetic materials.A high-throughput virtual screening(HTVS)system integrating on-demand molecular generation and machine learning models covering the prediction of molecular properties and crystal packing mode scoring is established.With the proposed HTVS system,candidate molecules with promising properties and a desirable crystal packing mode are rapidly targeted from the generated molecular space containing 25112 molecules.Furthermore,a study of the crystal structure and properties shows that the good comprehensive performances of the target molecule are in agreement with the predicted results,thus verifying the effectiveness of the proposed methodology.This work demonstrates a new research paradigm for discovering novel energetic materials and can be extended to other organic materials without manifest obstacles.
基金Supported by the Natural Science Foundation of Guangxi Province,China(Nos.0639025 and 0991084)the Support Program for 100 Young and Middle-aged Disciplinary Leaders in Higher Education Institutions of Guangxi Province,China(No. RC20060703005)+2 种基金the Key Laboratory of Development and Application of Forest Chemicals of Guangxi Province,China (No.GXFC08-06)the Education Department of Guangxi Province,China(No.200807MS074)the Innovation Project of Guangxi University fot Nationalities,China(No.gxun-chx2009081)
文摘A novel third-generation hydrogen peroxide（H2O2） biosensor（Hb/CdS/MWNTs/GCE） was fabricated through hemoglobin（Hb） adsorbed onto the mercaptoacetic acid modified CdS QDs/carboxyl multiwall carbon nanotubes＇（MWNTs） films. Cyclic voltammogram of Hb/CdS/MWNTs/GCE showed a pair of well-defined and quasi-reversible redox peaks with a formal potential（E^0） of-0.230 V（vs. Ag/AgCl） in 0.1 mol/L pH=8.0 phosphate buffer solution（PBS）, which was the characteristic of the Hb heme Fe（Ⅲ）/Fe（Ⅱ） redox couple. The biosensor shows an excellent electrocatalytic activity to the reduction of H2O2. The response time of the designed biosensor to H202 at a potential of-0.30 V was less than 2 s and linear relationships were obtained in the concentration ranges of 2.0×10^-6-2.7×10^-3 mol/L and 2.7×10^-3-7.7×10^-3 mol/L with a detection limit of 3.0×10^-7 mol/L（S/N=3）. The apparent Michaelis-Menten constant Km was estimated to be 1.324 mmol/L that illustrated the excellent biological activity of the fixed Hb.
基金National Natural Science Foundation of China(U160312581473330+5 种基金8187276881673323)Fundamental Research Funds for the Central Universities of China(N182008004N182006001)Liaoning Revitalization Talents Program(XLYC1807118)Liaoning BaiQianWan Talents Program(2018)
文摘OBJECTIVE Learning and memory impairment is one of the common sequelae of stroke patients,which is called"post-stroke dementia"and seriously affects the quality of life of the patients.For post-stroke dementia,there is still no effective clinical treatment.In the present study,we aim to investigate the effect of the active ingredient of Ferula sinkiangensis,AW09,on global cerebral ischemia-reperfusion mice.METHODS The bilateral common carotid artery occlusion(BCCAO)reperfusion model was used to investigate the protective effect of AW09 on cognitive dysfunction in mice with global cerebral ischemia reperfusion.Y-maze and Morris water maze were used to test the learning and memory ability of mice.RESULTS Y-maze test showed that AW09 treatment significantly increased the spontaneous alternation rate of BCCAO model animals and had no significant effect on the total number of arm entries.The results of Morris water maze showed that AW09 significantly reduced the escape latency of BCCAO mice during the training period.During the probe test phase,AW09 significantly increased the swimming time in target quadrant,distance in target quadrant and number of platform crossings and decreased the swimming time in the quadrant opposite the target quadrant of BCCAO mice.CONCLUSION AW09,the active ingredient of Ferula sinkiangensis,can improve working memory impairment and spatial memory impairment in animals with global cerebral ischemia-reperfusion,suggesting that AW09 has poten⁃tial therapeutic value for cognitive dysfunction caused by global cerebral ischemia.
