Current cell-based biosensors have progressed substantially from mere alternatives to molecular bioreceptors into enabling tools for interfacing molecular machineries and gene circuits with microelectronics and for de...Current cell-based biosensors have progressed substantially from mere alternatives to molecular bioreceptors into enabling tools for interfacing molecular machineries and gene circuits with microelectronics and for developing groundbreaking sensing and theragnostic platforms.The recent literature concerning whole-cell biosensors is reviewed with an emphasis on mammalian cells,and the challenges and breakthroughs brought along in biomedical analyses through novel biosensing concepts and the synthetic biology toolbox.These recent innovations allow development of cell-based biosensing platforms having tailored performances and capable to reach the levels of sensitivity,dynamic range,and stability suitable for high analytic/medical relevance.They also pave the way for the construction of flexible biosensing platforms with utility across biological research and clinical applications.The work is intended to stimulate interest in generation of cell-based biosensors and improve their acceptance and exploitation.展开更多
Retrieving electrical impedance maps at the nanoscale rapidly via nondestructive inspection with a high signal-to-noise ratio is an unmet need,likely to impact various applications from biomedicine to energy conversio...Retrieving electrical impedance maps at the nanoscale rapidly via nondestructive inspection with a high signal-to-noise ratio is an unmet need,likely to impact various applications from biomedicine to energy conversion.In this study,we develop a multimodal functional imaging instrument that is characterized by the dual capability of impedance mapping and phase quantitation,high spatial resolution,and low temporal noise.To achieve this,we advance a quantitative phase imaging system,referred to as epi-magnified image spatial spectrum microscopy combined with electrical actuation,to provide complementary maps of the optical path and electrical impedance.We demonstrate our system with high-resolution maps of optical path differences and electrical impedance variations that can distinguish nanosized,semi-transparent,structured coatings involving two materials with relatively similar electrical properties.We map heterogeneous interfaces corresponding to an indium tin oxide layer exposed by holes with diameters as small as~550 nm in a titanium(dioxide)over-layer deposited on a glass support.We show that electrical modulation during the phase imaging of a macro-electrode is decisive for retrieving electrical impedance distributions with submicron spatial resolution-and beyond the limitations of electrode-based technologies(surface or scanning technologies).The findings,which are substantiated by a theoretical model that fits the experimental data very well enable achieving electro-optical maps with high spatial and temporal resolutions.The virtues and limitations of the novel optoelectrochemical method that provides grounds for a wider range of electrically modulated optical methods for measuring the electric field locally are critically discussed.展开更多
文摘Current cell-based biosensors have progressed substantially from mere alternatives to molecular bioreceptors into enabling tools for interfacing molecular machineries and gene circuits with microelectronics and for developing groundbreaking sensing and theragnostic platforms.The recent literature concerning whole-cell biosensors is reviewed with an emphasis on mammalian cells,and the challenges and breakthroughs brought along in biomedical analyses through novel biosensing concepts and the synthetic biology toolbox.These recent innovations allow development of cell-based biosensing platforms having tailored performances and capable to reach the levels of sensitivity,dynamic range,and stability suitable for high analytic/medical relevance.They also pave the way for the construction of flexible biosensing platforms with utility across biological research and clinical applications.The work is intended to stimulate interest in generation of cell-based biosensors and improve their acceptance and exploitation.
基金the Romanian Executive Unit for Higher Education,Research,Development and Innovation Funding for funding through Grants ERANET Euronanomed(NanoLight,135),Permed(POC4Allergies,138),ERANET-M-(SmartMatter,173)The support of the Attract project funded by the EC(HORIZON 2020-Grant Agreement no.777222)+1 种基金The support of Fonds europeen de developpement regional(FEDER)and the Walloon region under the Operational Program“Wallonia-2020.EU”(project CLEARPOWER)is gratefully acknowledged.G.P.,M.E.K.,H M,received funding from EBICS(US NSF,0939511)supported by MBM(US NSF,NRT-UtB,1735252)GP is grateful to NSF(0939511)and NIH(R01-GM129709 and R01-CA238191)。
文摘Retrieving electrical impedance maps at the nanoscale rapidly via nondestructive inspection with a high signal-to-noise ratio is an unmet need,likely to impact various applications from biomedicine to energy conversion.In this study,we develop a multimodal functional imaging instrument that is characterized by the dual capability of impedance mapping and phase quantitation,high spatial resolution,and low temporal noise.To achieve this,we advance a quantitative phase imaging system,referred to as epi-magnified image spatial spectrum microscopy combined with electrical actuation,to provide complementary maps of the optical path and electrical impedance.We demonstrate our system with high-resolution maps of optical path differences and electrical impedance variations that can distinguish nanosized,semi-transparent,structured coatings involving two materials with relatively similar electrical properties.We map heterogeneous interfaces corresponding to an indium tin oxide layer exposed by holes with diameters as small as~550 nm in a titanium(dioxide)over-layer deposited on a glass support.We show that electrical modulation during the phase imaging of a macro-electrode is decisive for retrieving electrical impedance distributions with submicron spatial resolution-and beyond the limitations of electrode-based technologies(surface or scanning technologies).The findings,which are substantiated by a theoretical model that fits the experimental data very well enable achieving electro-optical maps with high spatial and temporal resolutions.The virtues and limitations of the novel optoelectrochemical method that provides grounds for a wider range of electrically modulated optical methods for measuring the electric field locally are critically discussed.
