Although vaccines have been developed,mutations of SARS-CoV-2,especially the dominant B.1.617.2(delta)and B.1.529(omicron)strains with more than 30 mutations on their spike protein,have caused a significant decline in...Although vaccines have been developed,mutations of SARS-CoV-2,especially the dominant B.1.617.2(delta)and B.1.529(omicron)strains with more than 30 mutations on their spike protein,have caused a significant decline in prophylaxis,calling for the need for drug improvement.Antibodies are drugs preferentially used in infectious diseases and are easy to get from immunized organisms.The current study combined molecular modeling and single memory B cell sequencing to assess candidate sequences before experiments,providing a strategy for the fabrication of SARS-CoV-2 neutralizing antibodies.A total of 128 sequences were obtained after sequencing 196 memory B cells,and 42 sequences were left after merging extremely similar ones and discarding incomplete ones,followed by homology modeling of the antibody variable region.Thirteen candidate sequences were expressed,of which three were tested positive for receptor binding domain recognition but only one was confirmed as having broad neutralization against several SARS-CoV-2 variants.The current study successfully obtained a SARS-CoV-2 antibody with broad neutralizing abilities and provided a strategy for antibody development in emerging infectious diseases using single memory B cell BCR sequencing and computer assistance in antibody fabrication.展开更多
The role played by cytokines, other than interferon （IFN）-a, in the differentiation and function of dendritic cells （DCs） in systemic lupus erythematosus （SLE）, remains unclear. Serum interleukin-10 （IL-10） le...The role played by cytokines, other than interferon （IFN）-a, in the differentiation and function of dendritic cells （DCs） in systemic lupus erythematosus （SLE）, remains unclear. Serum interleukin-10 （IL-10） levels are generally elevated in SLE patients, which might modulate the differentiation of DCs. In this study, DCs were induced from monocytes either by transendothelial trafficking or by culture with granulocyte-macrophage colony-stimulating factor （GM-CSF） ＋ IL-4 ＋ tumor necrosis factor （TNF）-a. Both systems were used to investigate the effects of elevated serum IL-10 level on DC differentiation in SLE patients. The results showed that monocyte-derived DCs induced by either SLE serum or exogenous IL-10 reduced the expression of human leukocyte antigen （HLA）-DR and CD80, decreased IL-12p40 level, and increased IL-10 level, and exhibited an impaired capacity to stimulate allogenic T-cell proliferation. These results indicate that serum IL-10 may be involved in the pathogenesis of SLE by modulating the differentiation and function of DCs.展开更多
Environmental pollutants,such as bisphenol A(BPA) have recently been implicated in the development of adverse birth outcomes.However,the underlying teratogenic mechanisms remain unclear.We investigated the effects of ...Environmental pollutants,such as bisphenol A(BPA) have recently been implicated in the development of adverse birth outcomes.However,the underlying teratogenic mechanisms remain unclear.We investigated the effects of BPA on the migration and invasion of human primary extravillous trophoblast HTR-8/SVneo cells.Our results indicated that BPA reduced cell migration and invasion.Moreover,it altered the ratio of matrix metalloproteinases(MMPs) and tissue inhibitors of MMPs(TIMPs) by downregulating MMP-2 and MMP-9,and upregulating TIMP-1 and TIMP-2.Furthermore,BPA suppressed integrin β1,integrin α5,and vimentin.Interestingly,BPA-induced invasion was partially restored by G15,a membrane G-protein-coupled estrogen receptor 30 antagonist.We further revealed that 42 proteins were differentially expressed by mass spectrometry analysis,which could be divided into three categories based on gene ontology including biological process,cellular component,and molecular function.These results suggest that BPA reduces HTR-8/SVneo cell migration and invasion by downregulating MMP-2 and MMP-9,up-regulating TIMP-1 and TIMP-2,and suppressing adhesion molecules.展开更多
In this paper, we assess the existing seven local pilot carbon emission trading schemes in China and analyse the factors determining whether China’s carbon market is successful in terms of handling substantial amount...In this paper, we assess the existing seven local pilot carbon emission trading schemes in China and analyse the factors determining whether China’s carbon market is successful in terms of handling substantial amounts of CO2 emissions rights, regulating the market and trading them at a reasonable price. The emission trading system is developing slowly in most of the participating provinces and cities. Prices tend to decline, while volumes trading slowly increase. The volatility is partially the result of regulation (the rights need to be renewed before a certain date) and partially due to government interventions in the market. Based on the assessment, recommendations are provided for China implementing a national carbon market, based on the experiences and lessons learnt from the seven local carbon emission trading schemes. Conditions for China to roll out the system and later improve the national emission trading scheme to replace the existing local emission trading schemes are formulated.展开更多
Unpredictable power fluctuation and fault ridethrough capability attract increased attention as two uncertain major factors in doubly-fed induction generators(DFIGs)integrated DC power system.Present solutions usually...Unpredictable power fluctuation and fault ridethrough capability attract increased attention as two uncertain major factors in doubly-fed induction generators(DFIGs)integrated DC power system.Present solutions usually require complicated cooperation comprising multiple modules of energy storage,current control,and voltage stabilizer.To overcome the drawbacks of existing solutions,this paper proposes a superconducting magnetic energy storage(SMES)integrated currentsource DC/DC converter(CSDC).It is mainly composed of a current-source back-to-back converter,and the SMES is tactfully embedded in series with the intermediate DC link.The proposed SMES-CSDC is installed in front of the DC-DFIG to carry out its dual abilities of load voltage stabilization under multifarious transient disturbances and power regulation under wind speed variations.Compared with the existing DC protection devices,the SMES-CSDC is designed on the basis of unique current-type energy storage.It has the advantages of fast response,extensive compensation range,concise hardware structure,and straightforward control strategy.The feasibility of the SMESCSDC is implemented via a scaled-down experiment,and its effectiveness for DC-DFIG protection is verified by a large-scale DC power system simulation.展开更多
In DC micro grids and networks,DC-DC power converters having a large number of semiconductor-based power electronic devices are usually adopted to interconnect the renewable sources and flexible loads.Most of the semi...In DC micro grids and networks,DC-DC power converters having a large number of semiconductor-based power electronic devices are usually adopted to interconnect the renewable sources and flexible loads.Most of the semiconductor-based devices suffer from poor fault withstanding abilities,but conventional power electronic protection schemes have the bottlenecks of the time-delay,self-malfunction and mis-judgement.This paper presents a novel solution using the superconducting fault current limiter(SFCL)to protect a power electronic device and extend the usage to a micro grid.This SFCL is actually a self-triggering,recoverable,and passive current limiter,which does not involve any additional circuit hardware and software.Experimental investigations and simulation analyses clarify the feasibility of using this superconductor-based protection scheme to implement the self-acting fail-safe protection of DC-DC converters.Further system-level simulations explore the SFCL to suppress the over-current and stabilize the bus voltage of a photovoltaic based DC micro grid,particularly facing millisecond-level transients and faults.Our experimental and theoretical investigations lay some technical bases to establish a superconductor-semiconductor-coupled interdisciplinary application from the view from the applied superconductivity,to power electronics,and to micro grids.展开更多
基金supported by the Jiangsu Provincial Key Research and Development Program (Grant No.BE2020616)the National Key R&D Program of China (Grant No.2018YFC1200603)+1 种基金the National Science and Technology Major Project (Grant No.2019SWAQ05-5-4)Jiangsu Key Lab of Cancer Biomarkers,Prevention and Treatment,Collaborative Innovation Center for Cancer Personalized Medicine,Nanjing Medical University.
文摘Although vaccines have been developed,mutations of SARS-CoV-2,especially the dominant B.1.617.2(delta)and B.1.529(omicron)strains with more than 30 mutations on their spike protein,have caused a significant decline in prophylaxis,calling for the need for drug improvement.Antibodies are drugs preferentially used in infectious diseases and are easy to get from immunized organisms.The current study combined molecular modeling and single memory B cell sequencing to assess candidate sequences before experiments,providing a strategy for the fabrication of SARS-CoV-2 neutralizing antibodies.A total of 128 sequences were obtained after sequencing 196 memory B cells,and 42 sequences were left after merging extremely similar ones and discarding incomplete ones,followed by homology modeling of the antibody variable region.Thirteen candidate sequences were expressed,of which three were tested positive for receptor binding domain recognition but only one was confirmed as having broad neutralization against several SARS-CoV-2 variants.The current study successfully obtained a SARS-CoV-2 antibody with broad neutralizing abilities and provided a strategy for antibody development in emerging infectious diseases using single memory B cell BCR sequencing and computer assistance in antibody fabrication.
基金supported by grants from Science Research Foundation of Ministry of Education of China (No. 205057)Foundation of Jiangsu Province Natural Science (No. 2004148)
文摘The role played by cytokines, other than interferon （IFN）-a, in the differentiation and function of dendritic cells （DCs） in systemic lupus erythematosus （SLE）, remains unclear. Serum interleukin-10 （IL-10） levels are generally elevated in SLE patients, which might modulate the differentiation of DCs. In this study, DCs were induced from monocytes either by transendothelial trafficking or by culture with granulocyte-macrophage colony-stimulating factor （GM-CSF） ＋ IL-4 ＋ tumor necrosis factor （TNF）-a. Both systems were used to investigate the effects of elevated serum IL-10 level on DC differentiation in SLE patients. The results showed that monocyte-derived DCs induced by either SLE serum or exogenous IL-10 reduced the expression of human leukocyte antigen （HLA）-DR and CD80, decreased IL-12p40 level, and increased IL-10 level, and exhibited an impaired capacity to stimulate allogenic T-cell proliferation. These results indicate that serum IL-10 may be involved in the pathogenesis of SLE by modulating the differentiation and function of DCs.
基金supported by the National Basic Research Program of China(973 Program,No.2014CB943303)。
文摘Environmental pollutants,such as bisphenol A(BPA) have recently been implicated in the development of adverse birth outcomes.However,the underlying teratogenic mechanisms remain unclear.We investigated the effects of BPA on the migration and invasion of human primary extravillous trophoblast HTR-8/SVneo cells.Our results indicated that BPA reduced cell migration and invasion.Moreover,it altered the ratio of matrix metalloproteinases(MMPs) and tissue inhibitors of MMPs(TIMPs) by downregulating MMP-2 and MMP-9,and upregulating TIMP-1 and TIMP-2.Furthermore,BPA suppressed integrin β1,integrin α5,and vimentin.Interestingly,BPA-induced invasion was partially restored by G15,a membrane G-protein-coupled estrogen receptor 30 antagonist.We further revealed that 42 proteins were differentially expressed by mass spectrometry analysis,which could be divided into three categories based on gene ontology including biological process,cellular component,and molecular function.These results suggest that BPA reduces HTR-8/SVneo cell migration and invasion by downregulating MMP-2 and MMP-9,up-regulating TIMP-1 and TIMP-2,and suppressing adhesion molecules.
文摘In this paper, we assess the existing seven local pilot carbon emission trading schemes in China and analyse the factors determining whether China’s carbon market is successful in terms of handling substantial amounts of CO2 emissions rights, regulating the market and trading them at a reasonable price. The emission trading system is developing slowly in most of the participating provinces and cities. Prices tend to decline, while volumes trading slowly increase. The volatility is partially the result of regulation (the rights need to be renewed before a certain date) and partially due to government interventions in the market. Based on the assessment, recommendations are provided for China implementing a national carbon market, based on the experiences and lessons learnt from the seven local carbon emission trading schemes. Conditions for China to roll out the system and later improve the national emission trading scheme to replace the existing local emission trading schemes are formulated.
基金supported by the National Natural Science Foundation of China(No.51807128)。
文摘Unpredictable power fluctuation and fault ridethrough capability attract increased attention as two uncertain major factors in doubly-fed induction generators(DFIGs)integrated DC power system.Present solutions usually require complicated cooperation comprising multiple modules of energy storage,current control,and voltage stabilizer.To overcome the drawbacks of existing solutions,this paper proposes a superconducting magnetic energy storage(SMES)integrated currentsource DC/DC converter(CSDC).It is mainly composed of a current-source back-to-back converter,and the SMES is tactfully embedded in series with the intermediate DC link.The proposed SMES-CSDC is installed in front of the DC-DFIG to carry out its dual abilities of load voltage stabilization under multifarious transient disturbances and power regulation under wind speed variations.Compared with the existing DC protection devices,the SMES-CSDC is designed on the basis of unique current-type energy storage.It has the advantages of fast response,extensive compensation range,concise hardware structure,and straightforward control strategy.The feasibility of the SMESCSDC is implemented via a scaled-down experiment,and its effectiveness for DC-DFIG protection is verified by a large-scale DC power system simulation.
基金the National Natural Science Foundation of China[Grant No.51807128].
文摘In DC micro grids and networks,DC-DC power converters having a large number of semiconductor-based power electronic devices are usually adopted to interconnect the renewable sources and flexible loads.Most of the semiconductor-based devices suffer from poor fault withstanding abilities,but conventional power electronic protection schemes have the bottlenecks of the time-delay,self-malfunction and mis-judgement.This paper presents a novel solution using the superconducting fault current limiter(SFCL)to protect a power electronic device and extend the usage to a micro grid.This SFCL is actually a self-triggering,recoverable,and passive current limiter,which does not involve any additional circuit hardware and software.Experimental investigations and simulation analyses clarify the feasibility of using this superconductor-based protection scheme to implement the self-acting fail-safe protection of DC-DC converters.Further system-level simulations explore the SFCL to suppress the over-current and stabilize the bus voltage of a photovoltaic based DC micro grid,particularly facing millisecond-level transients and faults.Our experimental and theoretical investigations lay some technical bases to establish a superconductor-semiconductor-coupled interdisciplinary application from the view from the applied superconductivity,to power electronics,and to micro grids.
