Background:B-cell CLL/lymphoma 6(BCL6)is a transcriptional master regulator that represses more than 1200 potential target genes.Our previous study showed that a decline in blood production in runting and stunting syn...Background:B-cell CLL/lymphoma 6(BCL6)is a transcriptional master regulator that represses more than 1200 potential target genes.Our previous study showed that a decline in blood production in runting and stunting syndrome(RSS)affected sex-linked dwarf(SLD)chickens compared to SLD chickens.However,the association between BCL6 gene and hematopoietic function remains unknown in chickens.Methods:In this study,we used RSS affected SLD(RSS-SLD)chickens,SLD chickens and normal chickens as research object and overexpression of BCL6 in hematopoietic stem cells(HSCs),to investigate the effect of the BCL6 on differentiation and development of HSCs.Results:The results showed that comparison of RSS-SLD chickens with SLD chickens,the BCL6 was highly expressed in RSS-SLD chickens bone marrow.The bone marrow of RSS-SLD chickens was exhausted and red bone marrow was largely replaced by yellow bone marrow,bone density was reduced,and the levels of immature erythrocytes in peripheral blood were increased.At the same time,the hematopoietic function of HSCs decreased in RSS-SLD chickens,which was manifested by a decrease in the hematopoietic growth factors(HGFs)EPO,SCF,TPO,and IL-3,as well as hemoglobinα1 and hemoglobinβexpression.Moreover,mitochondrial function in the HSCs of RSS-SLD chickens was damaged,including an increase in ROS production,decrease in ATP concentration,and decrease in mitochondrial membrane potential(ΔΨm).The same results were also observed in SLD chickens compared with normal chickens;however,the symptoms were more serious in RSS-SLD chickens.Additionally,after overexpression of the BCL6 in primary HSCs,the secretion of HGFs(EPO,SCF,TPO and IL-3)was inhibited and the expression of hemoglobinα1 and hemoglobinβwas decreased.However,cell proliferation was accelerated,apoptosis was inhibited,and the HSCs entered a cancerous state.The function of mitochondria was also abnormal,ROS production was decreased,and ATP concentration andΔΨm were increased,which was related to the inhibition of apoptosis of stem cells.Conclusions:Taken together,we conclude that the high expression of BCL6 inhibits the differentiation and development of HSCs by affecting mitochondrial function,resulting in impaired growth and development of chickens.Moreover,the abnormal expression of BCL6 might be a cause of the clinical manifestations of chicken comb,pale skin,stunted growth and development,and the tendency to appear RSS in SLD chickens.展开更多
为探索以“华侨华人”为主题词的国际学术研究脉络,借助文献计量可视化软件CiteSpace,对收录在Web of Science核心数据库(2010—2019)十年内的学术语篇进行定量文本挖掘与定性文本细读。结果表明:多学科跨学科的华侨华人研究得到全球性...为探索以“华侨华人”为主题词的国际学术研究脉络,借助文献计量可视化软件CiteSpace,对收录在Web of Science核心数据库(2010—2019)十年内的学术语篇进行定量文本挖掘与定性文本细读。结果表明:多学科跨学科的华侨华人研究得到全球性的关注和支持;“全球化”“身份”“态度”“高等教育”“学生”“人才”等成为研究热词;全景式的“中国人才流动”与“理解非洲叙事”这两个主题领衔研究前沿;华侨华人身心健康得到高度关注;国内学者在发文数量和前沿观点上把握了国际学术话语权,而高被引作者的相对缺失则说明话语能力与影响力还可望进一步提升。希望该研究能为华侨华人学术研究与实际工作提供有益的参考。展开更多
基金This work was supported by grants from the Key-Area Research and Development Program of Guangdong Province(Grant No.2020B020222002)the Guangdong Provincial Promotion Project on Preservation and Utilization of Local Breed of Livestock and Poultry,National Natural Science Foundation of China(Grant No.31401046)+1 种基金the China Agriculture Research System(CARS-41-G03)Guangdong Youth Talent Project.
文摘Background:B-cell CLL/lymphoma 6(BCL6)is a transcriptional master regulator that represses more than 1200 potential target genes.Our previous study showed that a decline in blood production in runting and stunting syndrome(RSS)affected sex-linked dwarf(SLD)chickens compared to SLD chickens.However,the association between BCL6 gene and hematopoietic function remains unknown in chickens.Methods:In this study,we used RSS affected SLD(RSS-SLD)chickens,SLD chickens and normal chickens as research object and overexpression of BCL6 in hematopoietic stem cells(HSCs),to investigate the effect of the BCL6 on differentiation and development of HSCs.Results:The results showed that comparison of RSS-SLD chickens with SLD chickens,the BCL6 was highly expressed in RSS-SLD chickens bone marrow.The bone marrow of RSS-SLD chickens was exhausted and red bone marrow was largely replaced by yellow bone marrow,bone density was reduced,and the levels of immature erythrocytes in peripheral blood were increased.At the same time,the hematopoietic function of HSCs decreased in RSS-SLD chickens,which was manifested by a decrease in the hematopoietic growth factors(HGFs)EPO,SCF,TPO,and IL-3,as well as hemoglobinα1 and hemoglobinβexpression.Moreover,mitochondrial function in the HSCs of RSS-SLD chickens was damaged,including an increase in ROS production,decrease in ATP concentration,and decrease in mitochondrial membrane potential(ΔΨm).The same results were also observed in SLD chickens compared with normal chickens;however,the symptoms were more serious in RSS-SLD chickens.Additionally,after overexpression of the BCL6 in primary HSCs,the secretion of HGFs(EPO,SCF,TPO and IL-3)was inhibited and the expression of hemoglobinα1 and hemoglobinβwas decreased.However,cell proliferation was accelerated,apoptosis was inhibited,and the HSCs entered a cancerous state.The function of mitochondria was also abnormal,ROS production was decreased,and ATP concentration andΔΨm were increased,which was related to the inhibition of apoptosis of stem cells.Conclusions:Taken together,we conclude that the high expression of BCL6 inhibits the differentiation and development of HSCs by affecting mitochondrial function,resulting in impaired growth and development of chickens.Moreover,the abnormal expression of BCL6 might be a cause of the clinical manifestations of chicken comb,pale skin,stunted growth and development,and the tendency to appear RSS in SLD chickens.
文摘为探索以“华侨华人”为主题词的国际学术研究脉络,借助文献计量可视化软件CiteSpace,对收录在Web of Science核心数据库(2010—2019)十年内的学术语篇进行定量文本挖掘与定性文本细读。结果表明:多学科跨学科的华侨华人研究得到全球性的关注和支持;“全球化”“身份”“态度”“高等教育”“学生”“人才”等成为研究热词;全景式的“中国人才流动”与“理解非洲叙事”这两个主题领衔研究前沿;华侨华人身心健康得到高度关注;国内学者在发文数量和前沿观点上把握了国际学术话语权,而高被引作者的相对缺失则说明话语能力与影响力还可望进一步提升。希望该研究能为华侨华人学术研究与实际工作提供有益的参考。
