Background:Very low-protein(VLP)diets negatively impact calcium(Ca)metabolism and absorption.The objective of this study was to investigate the effect of supplemental branched-chain amino acids(BCAA)and limiting amino...Background:Very low-protein(VLP)diets negatively impact calcium(Ca)metabolism and absorption.The objective of this study was to investigate the effect of supplemental branched-chain amino acids(BCAA)and limiting amino acids(LAA)on Ca digestibility,absorption and reabsorption in pigs fed with VLP diets.Forty-eight piglets were assigned to six treatments:positive control(PC),negative control(NC),and NC containing LAA 25%,LAA 50%,LAA+BCAA 25%(LB25)and LAA+BCAA 50%(LB50)more than recommendations.Results:Relative to PC or NC,LB25 and LB50 had higher digestibility of Ca and plasma Ca and phosphorus(P),but lower plasma vitamin D3.LB50 tended to increase vitamin D receptor transcript and protein in the gut,but decreased mRNA or protein abundance of parathyroid hormone 1 receptor(PTH1R),calbindin 1(CALB1),cytochrome P450 family 27 subfamily B member 1 and occludin in small intestine.LB50 increased the transcript of cytochrome P450 family 24 subfamily A member 1 and PTH1R but decreased the transcript of transient receptor potential cation channel subfamily V member 5,CALB1 and solute carrier family 17 member 4 in kidney.Conclusion:Overall,BCAA increased Ca digestibility through regulating the transcellular and paracellular Ca absorption in the gut and reabsorption in kidney during protein restriction.展开更多
Objective To examine the effect of acupuncture-like transcutaneous electrical nerve stimulation(ACUTENS)on labor pain in nulliparous women.Methods This randomized clinical trial was conducted at Razi Hospital in Qazvi...Objective To examine the effect of acupuncture-like transcutaneous electrical nerve stimulation(ACUTENS)on labor pain in nulliparous women.Methods This randomized clinical trial was conducted at Razi Hospital in Qazvin,Iran between January and July 2018.After the screening process,144 out of 184 pregnant women were selected by convenience sampling and assigned to an intervention group or a control group by the block randomization method.The intervention group received ACUTENS at Hegu(LI4),Shenmen(HT7),Sanyinjiao(SP6),and Neimadian(Extra)during various stages of delivery in addition to routine labor care at the delivery room.For the control group,the same routine labor care was performed alone.The severity of pain was assessed in the two groups using the visual analog scale(VAS).The data were analyzed using R software(ver.4.0.2)and repeated measurement analysis of variance.Results ACUTENS reduced the mean score of pain intensity in the first stage of labor in the intervention group more than that in the control group(P<0.001).Also,the mean scores of pain intensity in the second stage of labor,episiotomy,and one hour after delivery in the intervention group were significantly lower than those in the control group(P<0.001).Conclusion ACUTENS at Hegu(LI4),Shenmen(HT7),Sanyinjiao(SP6),and Neimadian(Extra)can decrease the intensity of labor pain in nulliparous women.It can be used as a non-pharmacological method for reducing pain in and after labor.展开更多
The objective of this study was to assess the growth efficiency of pigs fed with protein-restricted diets supplemented with branched-chain amino acids(BCAA)and limiting amino acids(LAA)above the rec-ommended levels.Fo...The objective of this study was to assess the growth efficiency of pigs fed with protein-restricted diets supplemented with branched-chain amino acids(BCAA)and limiting amino acids(LAA)above the rec-ommended levels.Following 2 weeks of adaptation,48 young barrows were weight matched and randomly assigned to 6 treatments(8 pigs/treatment)for 4 weeks:positive control(PC)with standard protein,negative control(NC)with very low protein containing LAA(i.e.,Lys,Met,Thr and Trp)at rec-ommended levels,and NC containing LAA 25%(L25),LAA 50%(L50),LAA+BCAA(i.e.,Leu,Ile and Val)25%(LB25)and LAA+BCAA 50%(LB50)more than recommendations.Feed intake(FI)and body weight(BW)were measured daily and weekly,respectively.At week 6,blood samples were collected,all pigs euthanized and tissue samples collected.The data were analyzed by univariate GLM or mixed procedure(SPSS)and the means were separated using paired Student's t-test followed by Benjamini-Hochberg correction.Relative to PC,NC had decreased FI,BW,unsupplemented plasma essential amino acids,serum insulin-like growth factor-I(IGF-I)and hypothalamic neuropeptide Y(NPY)(P<0.01).Compared to NC,L25 or L50,LB50 had increased BW and serum IGF-I and decreased plasma serotonin and both LB25 and LB50 had higher FI,plasma BCAA,hypothalamic 5-hydroxytryptamine-receptor 2A and NPY and jejunal 5-hydroxytryptamine-receptor 7(P<0.01).Overall,supplementation of protein-restricted diets with increased levels of dietary BCAA partially recovered the negative effects of these diets on growth through improved IGF-I concentration and FI,which was associated with changed expression of serotonin receptors,blood AA and hypothalamic NPY.展开更多
基金funded by Animal Health and Production and Animal Products:Improved Nutritional Performance,Growth,and Lactation of Animals,grant no. 2021–67016-33399/project accession no. 1024334Hatch project accession no. 1012889 from the USDA National Institute of Food and Agriculture (NIFA)
文摘Background:Very low-protein(VLP)diets negatively impact calcium(Ca)metabolism and absorption.The objective of this study was to investigate the effect of supplemental branched-chain amino acids(BCAA)and limiting amino acids(LAA)on Ca digestibility,absorption and reabsorption in pigs fed with VLP diets.Forty-eight piglets were assigned to six treatments:positive control(PC),negative control(NC),and NC containing LAA 25%,LAA 50%,LAA+BCAA 25%(LB25)and LAA+BCAA 50%(LB50)more than recommendations.Results:Relative to PC or NC,LB25 and LB50 had higher digestibility of Ca and plasma Ca and phosphorus(P),but lower plasma vitamin D3.LB50 tended to increase vitamin D receptor transcript and protein in the gut,but decreased mRNA or protein abundance of parathyroid hormone 1 receptor(PTH1R),calbindin 1(CALB1),cytochrome P450 family 27 subfamily B member 1 and occludin in small intestine.LB50 increased the transcript of cytochrome P450 family 24 subfamily A member 1 and PTH1R but decreased the transcript of transient receptor potential cation channel subfamily V member 5,CALB1 and solute carrier family 17 member 4 in kidney.Conclusion:Overall,BCAA increased Ca digestibility through regulating the transcellular and paracellular Ca absorption in the gut and reabsorption in kidney during protein restriction.
文摘Objective To examine the effect of acupuncture-like transcutaneous electrical nerve stimulation(ACUTENS)on labor pain in nulliparous women.Methods This randomized clinical trial was conducted at Razi Hospital in Qazvin,Iran between January and July 2018.After the screening process,144 out of 184 pregnant women were selected by convenience sampling and assigned to an intervention group or a control group by the block randomization method.The intervention group received ACUTENS at Hegu(LI4),Shenmen(HT7),Sanyinjiao(SP6),and Neimadian(Extra)during various stages of delivery in addition to routine labor care at the delivery room.For the control group,the same routine labor care was performed alone.The severity of pain was assessed in the two groups using the visual analog scale(VAS).The data were analyzed using R software(ver.4.0.2)and repeated measurement analysis of variance.Results ACUTENS reduced the mean score of pain intensity in the first stage of labor in the intervention group more than that in the control group(P<0.001).Also,the mean scores of pain intensity in the second stage of labor,episiotomy,and one hour after delivery in the intervention group were significantly lower than those in the control group(P<0.001).Conclusion ACUTENS at Hegu(LI4),Shenmen(HT7),Sanyinjiao(SP6),and Neimadian(Extra)can decrease the intensity of labor pain in nulliparous women.It can be used as a non-pharmacological method for reducing pain in and after labor.
基金funded by Animal Health and Production and Animal Products:Improved Nutritional Performance,Growth,and Lactation of Animals grant no.2018-67016-27471/project accession no.1014892,grant no.2021-67016-33399/project accession no.1024334Hatch project accession no.1012889 from the USDA National Institute of Food and Agriculture
文摘The objective of this study was to assess the growth efficiency of pigs fed with protein-restricted diets supplemented with branched-chain amino acids(BCAA)and limiting amino acids(LAA)above the rec-ommended levels.Following 2 weeks of adaptation,48 young barrows were weight matched and randomly assigned to 6 treatments(8 pigs/treatment)for 4 weeks:positive control(PC)with standard protein,negative control(NC)with very low protein containing LAA(i.e.,Lys,Met,Thr and Trp)at rec-ommended levels,and NC containing LAA 25%(L25),LAA 50%(L50),LAA+BCAA(i.e.,Leu,Ile and Val)25%(LB25)and LAA+BCAA 50%(LB50)more than recommendations.Feed intake(FI)and body weight(BW)were measured daily and weekly,respectively.At week 6,blood samples were collected,all pigs euthanized and tissue samples collected.The data were analyzed by univariate GLM or mixed procedure(SPSS)and the means were separated using paired Student's t-test followed by Benjamini-Hochberg correction.Relative to PC,NC had decreased FI,BW,unsupplemented plasma essential amino acids,serum insulin-like growth factor-I(IGF-I)and hypothalamic neuropeptide Y(NPY)(P<0.01).Compared to NC,L25 or L50,LB50 had increased BW and serum IGF-I and decreased plasma serotonin and both LB25 and LB50 had higher FI,plasma BCAA,hypothalamic 5-hydroxytryptamine-receptor 2A and NPY and jejunal 5-hydroxytryptamine-receptor 7(P<0.01).Overall,supplementation of protein-restricted diets with increased levels of dietary BCAA partially recovered the negative effects of these diets on growth through improved IGF-I concentration and FI,which was associated with changed expression of serotonin receptors,blood AA and hypothalamic NPY.
