Foreign body reactions to the wear debris and corrosion products from the implants,and bacterial infections are the main factors leading to the implant failures.In order to resolve these problems,the antibacterial TiN...Foreign body reactions to the wear debris and corrosion products from the implants,and bacterial infections are the main factors leading to the implant failures.In order to resolve these problems,the antibacterial TiN/Cu nanocomposite coatings with various N_(2) partial pressures were deposited on 304 stainless steels(SS)using an arc ion plating(AIP)system,named TiN/Cu-x(x=0.5,1.0,1.5 Pa).The results of X-ray diffraction analysis,energy-dispersive X-ray spectroscopy,and scanning electron microscopy showed that the N_(2) partial pressures determined the Cu contents,surface defects,and crystallite sizes of TiN/Cu nanocomposite coatings,which further influenced the comprehensive abilities.And the hardness and wear resistances of TiN/Cu coatings were enhanced with increase of the crystallite sizes.Under the co-actions of surface defects,crystallite sizes,and Cu content,TiN/Cu-1.0 and TiN/Cu-1.5 coatings possessed excellent corrosion resistance.Besides,the biological tests proved that all the TiN/Cu coatings showed no cytotoxicity with strong antibacterial ability.Among them,TiN/Cu-1.5 coating significantly promoted the cell proliferation,which is expected to be a novel antibacterial,corrosion-resistant,and wear-resistant coating on the surfaces of medical implants.展开更多
This research systematically examined the degradation,antibacterial effects,and biocompatibility of micro-arc oxidation(MAO)coatings with nano CuO and ZnO on extruded Mg alloys.Both copper(Cu)and Zinc(Zn)possess antib...This research systematically examined the degradation,antibacterial effects,and biocompatibility of micro-arc oxidation(MAO)coatings with nano CuO and ZnO on extruded Mg alloys.Both copper(Cu)and Zinc(Zn)possess antibacterial properties.The findings demonstrated that adding ZnO will appreciably reduce the degradation rate of MAO-coating alloy due to the self-sealing micro holes.CuO+MAO coating exhibited excellent antibacterial performance,with an antibacterial rate of over 90%within 6 h co-cultured with Staphylococcus aureus.Similarly,the antibacterial rate of ZnO+MAO coating reached 90%after 12 h co-culture.Cytotoxicity test using MG63 cell indicated that the incorporation of CuO and ZnO did not notably affect the cell viability rate of the coating.Moreover,after 14 days of culture,the CuO+MAO and ZnO+MAO coated samples exhibited higher alkaline phosphatase(ALP)activity than the MAO-coated and uncoated samples,suggesting favorable osteogenic properties.展开更多
Ti-15 Zr-xCu(3≤x≤7,wt.%) novel antibacterial and antibiofilm alloys with competitive mechanical properties,biological responses and corrosion resistance were designed and fabricated.Annealing heat treatment on Ti-15...Ti-15 Zr-xCu(3≤x≤7,wt.%) novel antibacterial and antibiofilm alloys with competitive mechanical properties,biological responses and corrosion resistance were designed and fabricated.Annealing heat treatment on Ti-15 Zr-7 Cu(TZC-7 A),after holding for 2 h at slightly above their beta transus temperature(BTT) ensured their tensile strength(UTS),yield strength(YS) and hardness(HRV) were improved by31.2%,20% and 12.3% respectively compared to the control without Cu,Ti-15 Zr(T-15 ZA).Although the3 wt.% Cu alloy displayed the highest elongation(26%),the TZC-7 A alloy also possessed a good ductility.Presence of evenly dispersed Ti2 Cu and Zr2 Cu Cu-rich intermetallic phases formed as interwoven and alternating lamellae within the α+β matrix as a result of Cu addition,as revealed by X-ray diffraction(XRD),scanning electron microscopy(SEM) and transmission electron microscopy(TEM).These greatly contributed to their strengthening and bactericidal properties.Over 98% antibacterial effect against E.coli and S.aureus have been imparted,coupled with excellent biofilm inhibition.Potentiodynamic polarization curves showed that the TZC-7 A alloy possessed higher corrosion resistance than commercially pure titanium,cp-Ti;contact angle test revealed enhanced hydrophilicity;while confocal laser scanning microscopy(CLSM) and cell counting kit(CCK-8) assays also displayed drastically lowered bacterial adhesion rate with comparatively no cytotoxicity.Cell attachment on all alloys was similar but the best spread was obtained on TZC-7 A after 24 h.The developed alloy has good potential as an antibacterial implant material with combination of optimized properties.展开更多
The adsorption behavior, antibacterial, and corrosion properties of a Ti-3 Cu alloy were studied in a phosphate-buffered saline solution containing 0, 1, 3, and 6 gL^(-1) bovine serum albumin protein at 37℃ and pH = ...The adsorption behavior, antibacterial, and corrosion properties of a Ti-3 Cu alloy were studied in a phosphate-buffered saline solution containing 0, 1, 3, and 6 gL^(-1) bovine serum albumin protein at 37℃ and pH = 7.4(±0.2). The protein adsorption behavior was examined via cyclic voltammetry, secondary ions mass spectroscopy(SIMS), and angle-resolved X-ray photoelectron spectroscopy(ARXPS). The corrosion property was analyzed by the open circuit potential(OCP), potentiodynamic polarization(PD),and electrochemical impedance spectroscopy(EIS) examinations. The antibacterial test was conducted according to the GB/T 21510 China Standard. It was observed that the surface charge density(QA DS) was directly proportional to the amount of the adsorbed BSA protein, signifying that the protein adsorption was accompanied by the charge transfer, pointing to chemisorptions phenomena. BSA amino groups and other organic species were observed in the surface analysis examinations. It was shown that the formation of barrier complexes between the TiO_(2) oxide-layer and PBS solution resulted in decreasing the release of Cu-ions, which consequently reduced the antibacterial activity. On the other hand, these barrier complexes improved the corrosion resistance by increasing the charge transfer resistance and double-layer capacitance of the Ti-3 Cu alloy.展开更多
The effects of different aging conditions on the microstructure,strength,corrosion resistance,cytotoxicity and antibacterial ability of Ti-15Zr-xCu(3≤x≤7,wt%)(TZC)alloys were systematically investigated.Microstructu...The effects of different aging conditions on the microstructure,strength,corrosion resistance,cytotoxicity and antibacterial ability of Ti-15Zr-xCu(3≤x≤7,wt%)(TZC)alloys were systematically investigated.Microstructural evolution and behavior were analyzed by X-ray diffraction(XRD)patterns and scanning electron microscopy with energy-dispersive spectroscopy(SEM-EDS),while potentiodynamic polarization technique was employed to characterize the corrosion response of the alloys after solution-treatment and aging(STA).High-temperature aging at 660℃ for 4 h(660-4)gave the best combination of properties by enabling significant precipitation of the Cu-rich Ti_(2)Cu and Zr_(2)Cu compounds,and mild formation of the Zr_(7)Cu_(10) secondary phase.The high kinetics at this condition was beneficial to the complete precipitation and more homogeneous distribution of the intermetallic particles.These led to the inhibition of dislocation movements and allowed for significantly improved mechanical strengths with added ductility,availability of more Cu ions for the desired oligodynamic activity without evoking cytotoxicity,better corrosion resistance and very high antibacterial ability(over 99.5%),thus improving the overall properties of the TZC alloys for biomedical applications.展开更多
基金financially supported by National Key Research and Development Program of China (Nos. 2018YFC1106601 and 2016YFC1100601)Liaoning Revitalization Talents Program (No. XLYC1807069)+1 种基金National Natural Science Foundation of China (Nos. 51631009 and 31870954)Key Projects for Foreign Cooperation of Bureau of International Cooperation Chinese Academy of Sciences (No. 174321KYSB20180006)
文摘Foreign body reactions to the wear debris and corrosion products from the implants,and bacterial infections are the main factors leading to the implant failures.In order to resolve these problems,the antibacterial TiN/Cu nanocomposite coatings with various N_(2) partial pressures were deposited on 304 stainless steels(SS)using an arc ion plating(AIP)system,named TiN/Cu-x(x=0.5,1.0,1.5 Pa).The results of X-ray diffraction analysis,energy-dispersive X-ray spectroscopy,and scanning electron microscopy showed that the N_(2) partial pressures determined the Cu contents,surface defects,and crystallite sizes of TiN/Cu nanocomposite coatings,which further influenced the comprehensive abilities.And the hardness and wear resistances of TiN/Cu coatings were enhanced with increase of the crystallite sizes.Under the co-actions of surface defects,crystallite sizes,and Cu content,TiN/Cu-1.0 and TiN/Cu-1.5 coatings possessed excellent corrosion resistance.Besides,the biological tests proved that all the TiN/Cu coatings showed no cytotoxicity with strong antibacterial ability.Among them,TiN/Cu-1.5 coating significantly promoted the cell proliferation,which is expected to be a novel antibacterial,corrosion-resistant,and wear-resistant coating on the surfaces of medical implants.
基金This work was supported by the National Natural Science Foundation of China(No.52001034)the China Postdoctoral Science Foundation(No.2023M731677)the Major Project of 2025 Sci&Tech Innovation of Ningbo(No.2020Z096).
文摘This research systematically examined the degradation,antibacterial effects,and biocompatibility of micro-arc oxidation(MAO)coatings with nano CuO and ZnO on extruded Mg alloys.Both copper(Cu)and Zinc(Zn)possess antibacterial properties.The findings demonstrated that adding ZnO will appreciably reduce the degradation rate of MAO-coating alloy due to the self-sealing micro holes.CuO+MAO coating exhibited excellent antibacterial performance,with an antibacterial rate of over 90%within 6 h co-cultured with Staphylococcus aureus.Similarly,the antibacterial rate of ZnO+MAO coating reached 90%after 12 h co-culture.Cytotoxicity test using MG63 cell indicated that the incorporation of CuO and ZnO did not notably affect the cell viability rate of the coating.Moreover,after 14 days of culture,the CuO+MAO and ZnO+MAO coated samples exhibited higher alkaline phosphatase(ALP)activity than the MAO-coated and uncoated samples,suggesting favorable osteogenic properties.
基金supported financially by the National Key Research and Development Program of China (Nos. 2018YFC1106601 and 2016YFC1100600)National Natural Science Foundation (No. 51631009)+2 种基金Youth Innovation Promotion Association, CAS (No. 2014168)Promoting Liaoning Province Talents Program-Top Young Talents (XLYC1807069)the support of CAS-TWAS President Fellowship。
文摘Ti-15 Zr-xCu(3≤x≤7,wt.%) novel antibacterial and antibiofilm alloys with competitive mechanical properties,biological responses and corrosion resistance were designed and fabricated.Annealing heat treatment on Ti-15 Zr-7 Cu(TZC-7 A),after holding for 2 h at slightly above their beta transus temperature(BTT) ensured their tensile strength(UTS),yield strength(YS) and hardness(HRV) were improved by31.2%,20% and 12.3% respectively compared to the control without Cu,Ti-15 Zr(T-15 ZA).Although the3 wt.% Cu alloy displayed the highest elongation(26%),the TZC-7 A alloy also possessed a good ductility.Presence of evenly dispersed Ti2 Cu and Zr2 Cu Cu-rich intermetallic phases formed as interwoven and alternating lamellae within the α+β matrix as a result of Cu addition,as revealed by X-ray diffraction(XRD),scanning electron microscopy(SEM) and transmission electron microscopy(TEM).These greatly contributed to their strengthening and bactericidal properties.Over 98% antibacterial effect against E.coli and S.aureus have been imparted,coupled with excellent biofilm inhibition.Potentiodynamic polarization curves showed that the TZC-7 A alloy possessed higher corrosion resistance than commercially pure titanium,cp-Ti;contact angle test revealed enhanced hydrophilicity;while confocal laser scanning microscopy(CLSM) and cell counting kit(CCK-8) assays also displayed drastically lowered bacterial adhesion rate with comparatively no cytotoxicity.Cell attachment on all alloys was similar but the best spread was obtained on TZC-7 A after 24 h.The developed alloy has good potential as an antibacterial implant material with combination of optimized properties.
基金financially supported by the National Key Research and Development Program of China(2018YFC1106601)Liaoning Revitalization Talents Program(XLYC1807069)+1 种基金National Natural Science Foundation(No.51631009,31870954)support of the CSC scholarship。
文摘The adsorption behavior, antibacterial, and corrosion properties of a Ti-3 Cu alloy were studied in a phosphate-buffered saline solution containing 0, 1, 3, and 6 gL^(-1) bovine serum albumin protein at 37℃ and pH = 7.4(±0.2). The protein adsorption behavior was examined via cyclic voltammetry, secondary ions mass spectroscopy(SIMS), and angle-resolved X-ray photoelectron spectroscopy(ARXPS). The corrosion property was analyzed by the open circuit potential(OCP), potentiodynamic polarization(PD),and electrochemical impedance spectroscopy(EIS) examinations. The antibacterial test was conducted according to the GB/T 21510 China Standard. It was observed that the surface charge density(QA DS) was directly proportional to the amount of the adsorbed BSA protein, signifying that the protein adsorption was accompanied by the charge transfer, pointing to chemisorptions phenomena. BSA amino groups and other organic species were observed in the surface analysis examinations. It was shown that the formation of barrier complexes between the TiO_(2) oxide-layer and PBS solution resulted in decreasing the release of Cu-ions, which consequently reduced the antibacterial activity. On the other hand, these barrier complexes improved the corrosion resistance by increasing the charge transfer resistance and double-layer capacitance of the Ti-3 Cu alloy.
基金financially supported by the National Natural Science Foundation of China(Nos.51631009 and 31870954).S.K.Kolawole graciously appreciates CAS-TWAS President Fellowship for the scholarship aid。
文摘The effects of different aging conditions on the microstructure,strength,corrosion resistance,cytotoxicity and antibacterial ability of Ti-15Zr-xCu(3≤x≤7,wt%)(TZC)alloys were systematically investigated.Microstructural evolution and behavior were analyzed by X-ray diffraction(XRD)patterns and scanning electron microscopy with energy-dispersive spectroscopy(SEM-EDS),while potentiodynamic polarization technique was employed to characterize the corrosion response of the alloys after solution-treatment and aging(STA).High-temperature aging at 660℃ for 4 h(660-4)gave the best combination of properties by enabling significant precipitation of the Cu-rich Ti_(2)Cu and Zr_(2)Cu compounds,and mild formation of the Zr_(7)Cu_(10) secondary phase.The high kinetics at this condition was beneficial to the complete precipitation and more homogeneous distribution of the intermetallic particles.These led to the inhibition of dislocation movements and allowed for significantly improved mechanical strengths with added ductility,availability of more Cu ions for the desired oligodynamic activity without evoking cytotoxicity,better corrosion resistance and very high antibacterial ability(over 99.5%),thus improving the overall properties of the TZC alloys for biomedical applications.
