【目的】大豆(Glycine max L.)含有丰富的植物蛋白和油脂,因而成为一种具有较高经济价值的作物。探究不同大豆品种的腐竹加工产量及不同大豆品种生产的腐竹在蛋白质、油分、可溶性糖、异黄酮之间的相关性,为制作生产高异黄酮腐竹提供参...【目的】大豆(Glycine max L.)含有丰富的植物蛋白和油脂,因而成为一种具有较高经济价值的作物。探究不同大豆品种的腐竹加工产量及不同大豆品种生产的腐竹在蛋白质、油分、可溶性糖、异黄酮之间的相关性,为制作生产高异黄酮腐竹提供参考依据。【方法】采用来自黑龙江和广东大豆产区的品种24份,用同一工艺制作腐竹,然后用凯氏定氮法测定大豆和腐竹中蛋白质,用索氏抽提法测定油分,用蒽酮比色法测定可溶性糖,用高效液相色谱法测定大豆和腐竹中的异黄酮含量。【结果】不同品种在腐竹产量和品质方面都存在明显的差异,大豆品种华夏8号制得腐竹产率最高,达到60.50%;其次为品种华春2号,为52.44%,这两个品种是制作腐竹的理想品种;此外,绥农37、华春6号和黑河43的腐竹生产率分别达到了48.59%、48.37%和47.91%,也是产率比较高的品种。相关分析结果表明,腐竹产量与大豆中蛋白质含量呈显著正相关(r=0.598**),与大豆中的可溶性糖呈负相关(r=-0.423*)。腐竹蛋白质含量、油分含量及异黄酮含量3个性状都分别与大豆种子对应的性状呈极显著正相关(r分别为0.700**、0.537**和0.879**);腐竹可溶性糖含量与大豆可溶性糖含量呈显著正相关(r=0.441*)。腐竹中的蛋白质含量与大豆可溶性糖含量呈极显著负相关(r=-0.519**)。腐竹中的油分含量与大豆中蛋白质呈极显著负相关(r=-0.889**),与大豆中可溶性糖和异黄酮含量呈极显著正相关(r分别为0.614**和0.574**);腐竹中异黄酮含量与大豆中蛋白质含量呈极显著负相关(r=-0.589**),与大豆中可溶性糖含量呈极显著正相关(r=0.568**)。【结论】大豆品种的腐竹产率和主要品质性状存在显著差异,其中,华夏8号、华春2号是制作腐竹的高产品种,大豆品种的品质特性决定了腐竹的品质特性,其主要由大豆品种的遗传特性决定的。展开更多
Soybean mosaic virus(SMV) affects seed quality and production of soybean(Glycine max(L.) Merr.) worldwide.SC18 is one of the dominant SMV strains in South China,and accession Zhonghuang 24 displayed resistance to SC18...Soybean mosaic virus(SMV) affects seed quality and production of soybean(Glycine max(L.) Merr.) worldwide.SC18 is one of the dominant SMV strains in South China,and accession Zhonghuang 24 displayed resistance to SC18.The F_(1),F_(2) and 168 F_(11) recombinant inbred lines(RILs) population derived from a hybridization between Zhonghuang 24(resistant,R) and Huaxia 3(susceptible,S) were used in this study.According to the segregation ratios of the F_(2) generation(3 R:1 S) and the recombinant inbred lines(RILs) population(1 R:1 S),one dominant locus may regulate the resistance to SC18 in Zhonghuang 24.By using composite interval mapping(CIM),Rsc18 was mapped to a 415.357-kb region on chromosome 13.Three candidate genes,including one NBS-LRR type gene and two serine/threonine protein type genes,were identified according to the genetic annotations,which may be related to the resistance to SC18.The q RT-PCR demonstrated that these genes were up-regulated in the R genotype compared to the control.In conclusion,the findings of this research enhanced the understanding about the R genes at the Rsc18 locus.Moreover,our results will provide insights for designing molecular markers to improve marker-assisted selection and developing new varieties with resistance to SC18.展开更多
Pod shattering is an important domesticated trait which can cause great economic loss of crop yield in cultivated soybean.In this study,we utilized two recombinant inbred line populations(RILs,CY,Huachun 2×Wayao;...Pod shattering is an important domesticated trait which can cause great economic loss of crop yield in cultivated soybean.In this study,we utilized two recombinant inbred line populations(RILs,CY,Huachun 2×Wayao;GB,Guizao 1×B13)to identify quantitative trait loci(QTLs)associated with pod shattering in soybean across multiple environments.A total of 14 QTLs for pod shattering were identified in the two RIL populations,which had LOD scores ranging from 2.64 to 44.33 with phenotypic variance explanation(PVE)ranging from 1.33 to 50.85%.One QTL qPS16-1,located on chromosome 16,included a well-known functional gene Pod dehiscence 1(Pdh1)that was reported previously.Ten new putative QTLs were validated in two RIL populations,and their LOD scores were between 2.55 and 4.24,explaining 1.33 to 2.60%of the phenotypic variation.Of which four novel QTLs(qPS01-1,qPS03-2,qPS05-1,and qPS07-1)could be detected in two environments where nine genes had specific changes in gene expression.Although the nine genes may have significant effects on pod shattering of soybean,their detailed functions still need to be further explored in the future.The results of this study will facilitate a better understanding of the genetic basis of the pod shattering-resistant trait and benefit soybean molecular breeding for improving pod shattering resistance.展开更多
In order to produce efficiently symbiotic system in acid soils which could be used for the recovery of insoluble phosphate soil and improvement of the fertility, etc., host range of Rhizobium was isolated from the roo...In order to produce efficiently symbiotic system in acid soils which could be used for the recovery of insoluble phosphate soil and improvement of the fertility, etc., host range of Rhizobium was isolated from the root nodules of wild and cultivated soybean varieties growing in south China, evaluated the effects of Rhizobium on enhance nitrogen fixing activities, phosphorus(P), nitrogen(N) uptakes, growth and yield of soybean under pot and field conditions. The results showed that combined Rhizobium inoculation was more effective than individual Rhizobium inoculation. The combined Rhizobium of CW54 strains had the highest effect, nodule number, nodule weight and nodule nitrogenase activity were markedly stimulated as compared to those of the control U110, respectively. The nitrogen contents in the plants treated with CW54 strains were significantly increased by 43.2% as compared to those of the control U110 in Al-P soil, but statistical significance was not observed in Fe-P soil. By contrast, inoculation with CW54 significantly enhanced phosphorus uptake of plant in Fe-P soil than that in Al-P soil. The effects of inoculation of CW54 strains in a field condition were similar to those of in a pot condition. The total biomass and yield in the plants treated with CW54 strains were significantly increased by 86.4% and 51.4% as compared to those of the control at the time of soybean podding in a field condition, respectively. These results suggested that inoculation Rhizobium was beneficial for soybean nutrient uptake, growth and yield under insoluble phosphate soil conditions.展开更多
Twenty five soybean strains of similar growth period were grown at eight locations simultanously in three northeast provinces of China.Most of the experimental sites differed markedly in temperature,precipitation, soi...Twenty five soybean strains of similar growth period were grown at eight locations simultanously in three northeast provinces of China.Most of the experimental sites differed markedly in temperature,precipitation, soil fertility and length of growing season.Protein and oil contents were detelmined to study the effects of ecological conditions on soybean chemical composition. The results showed that the average protein and protein + oil contents were higher at the south locations and lower at north locations, but there was no consistent variation for oil content,and there was no significant difference among most of the sites. Oil content of strains was highest when planted in Jiling. Combined analyses of variance indicated that there existed significant genotype,location and G×E interactions effects. F values of G×E interactions for all traits,although significant at 0.01 level of probability, were extremely smaller than those for location and genotype.This suggested that G×E interaction effects were relatively less important than both genotyp and location effects.Phenotypic correlations of the performance of genotypes,on single ehtry -basis,among eight locations were all positive for protein and protein+ oil and most of correlation coefficients were significant at 0.01 level of probability.This indicated that the relative performance of genotypes at each location was similar. In contrast,most of the correlation coefficients for oil content among the eight locations were not significant.Therefore,G×E interactions should be considered in breeding for high oil soybeans,as it may lead to the instability of genotypes and affect the rankings of genotypes when soybeans arc grown in diverse environments.展开更多
文摘【目的】大豆(Glycine max L.)含有丰富的植物蛋白和油脂,因而成为一种具有较高经济价值的作物。探究不同大豆品种的腐竹加工产量及不同大豆品种生产的腐竹在蛋白质、油分、可溶性糖、异黄酮之间的相关性,为制作生产高异黄酮腐竹提供参考依据。【方法】采用来自黑龙江和广东大豆产区的品种24份,用同一工艺制作腐竹,然后用凯氏定氮法测定大豆和腐竹中蛋白质,用索氏抽提法测定油分,用蒽酮比色法测定可溶性糖,用高效液相色谱法测定大豆和腐竹中的异黄酮含量。【结果】不同品种在腐竹产量和品质方面都存在明显的差异,大豆品种华夏8号制得腐竹产率最高,达到60.50%;其次为品种华春2号,为52.44%,这两个品种是制作腐竹的理想品种;此外,绥农37、华春6号和黑河43的腐竹生产率分别达到了48.59%、48.37%和47.91%,也是产率比较高的品种。相关分析结果表明,腐竹产量与大豆中蛋白质含量呈显著正相关(r=0.598**),与大豆中的可溶性糖呈负相关(r=-0.423*)。腐竹蛋白质含量、油分含量及异黄酮含量3个性状都分别与大豆种子对应的性状呈极显著正相关(r分别为0.700**、0.537**和0.879**);腐竹可溶性糖含量与大豆可溶性糖含量呈显著正相关(r=0.441*)。腐竹中的蛋白质含量与大豆可溶性糖含量呈极显著负相关(r=-0.519**)。腐竹中的油分含量与大豆中蛋白质呈极显著负相关(r=-0.889**),与大豆中可溶性糖和异黄酮含量呈极显著正相关(r分别为0.614**和0.574**);腐竹中异黄酮含量与大豆中蛋白质含量呈极显著负相关(r=-0.589**),与大豆中可溶性糖含量呈极显著正相关(r=0.568**)。【结论】大豆品种的腐竹产率和主要品质性状存在显著差异,其中,华夏8号、华春2号是制作腐竹的高产品种,大豆品种的品质特性决定了腐竹的品质特性,其主要由大豆品种的遗传特性决定的。
基金supported by the projects of the Key-Areas Research and Development Program of Guangdong Province, China (2020B020220008)the China Agriculture Research System of MOF and MARA (CARS-04-PSO9)+2 种基金the Major Project of New Varieties Cultivation of Genetically Modified Varieties, China (2016ZX08004002-007)the National Key R&D Program of China (2017FYD0101500)the National Natural Science Foundation of China (31971966)。
文摘Soybean mosaic virus(SMV) affects seed quality and production of soybean(Glycine max(L.) Merr.) worldwide.SC18 is one of the dominant SMV strains in South China,and accession Zhonghuang 24 displayed resistance to SC18.The F_(1),F_(2) and 168 F_(11) recombinant inbred lines(RILs) population derived from a hybridization between Zhonghuang 24(resistant,R) and Huaxia 3(susceptible,S) were used in this study.According to the segregation ratios of the F_(2) generation(3 R:1 S) and the recombinant inbred lines(RILs) population(1 R:1 S),one dominant locus may regulate the resistance to SC18 in Zhonghuang 24.By using composite interval mapping(CIM),Rsc18 was mapped to a 415.357-kb region on chromosome 13.Three candidate genes,including one NBS-LRR type gene and two serine/threonine protein type genes,were identified according to the genetic annotations,which may be related to the resistance to SC18.The q RT-PCR demonstrated that these genes were up-regulated in the R genotype compared to the control.In conclusion,the findings of this research enhanced the understanding about the R genes at the Rsc18 locus.Moreover,our results will provide insights for designing molecular markers to improve marker-assisted selection and developing new varieties with resistance to SC18.
基金supported by the Research and Development Program in the Key Areas of Guangdong Province,China(2020B020220008)the China Agriculture Research System of MOF and MARA(CARS-04-PS09)+1 种基金the Guangdong Agricultural Research System,China(2020KJ136)the Research Project of the State Key Laboratory of Agricultural and Biological ResourcesProtection and Utilization in Subtropics,China。
文摘Pod shattering is an important domesticated trait which can cause great economic loss of crop yield in cultivated soybean.In this study,we utilized two recombinant inbred line populations(RILs,CY,Huachun 2×Wayao;GB,Guizao 1×B13)to identify quantitative trait loci(QTLs)associated with pod shattering in soybean across multiple environments.A total of 14 QTLs for pod shattering were identified in the two RIL populations,which had LOD scores ranging from 2.64 to 44.33 with phenotypic variance explanation(PVE)ranging from 1.33 to 50.85%.One QTL qPS16-1,located on chromosome 16,included a well-known functional gene Pod dehiscence 1(Pdh1)that was reported previously.Ten new putative QTLs were validated in two RIL populations,and their LOD scores were between 2.55 and 4.24,explaining 1.33 to 2.60%of the phenotypic variation.Of which four novel QTLs(qPS01-1,qPS03-2,qPS05-1,and qPS07-1)could be detected in two environments where nine genes had specific changes in gene expression.Although the nine genes may have significant effects on pod shattering of soybean,their detailed functions still need to be further explored in the future.The results of this study will facilitate a better understanding of the genetic basis of the pod shattering-resistant trait and benefit soybean molecular breeding for improving pod shattering resistance.
基金Supported by National Natural Science Foundation of China(31371642)
文摘In order to produce efficiently symbiotic system in acid soils which could be used for the recovery of insoluble phosphate soil and improvement of the fertility, etc., host range of Rhizobium was isolated from the root nodules of wild and cultivated soybean varieties growing in south China, evaluated the effects of Rhizobium on enhance nitrogen fixing activities, phosphorus(P), nitrogen(N) uptakes, growth and yield of soybean under pot and field conditions. The results showed that combined Rhizobium inoculation was more effective than individual Rhizobium inoculation. The combined Rhizobium of CW54 strains had the highest effect, nodule number, nodule weight and nodule nitrogenase activity were markedly stimulated as compared to those of the control U110, respectively. The nitrogen contents in the plants treated with CW54 strains were significantly increased by 43.2% as compared to those of the control U110 in Al-P soil, but statistical significance was not observed in Fe-P soil. By contrast, inoculation with CW54 significantly enhanced phosphorus uptake of plant in Fe-P soil than that in Al-P soil. The effects of inoculation of CW54 strains in a field condition were similar to those of in a pot condition. The total biomass and yield in the plants treated with CW54 strains were significantly increased by 86.4% and 51.4% as compared to those of the control at the time of soybean podding in a field condition, respectively. These results suggested that inoculation Rhizobium was beneficial for soybean nutrient uptake, growth and yield under insoluble phosphate soil conditions.
文摘Twenty five soybean strains of similar growth period were grown at eight locations simultanously in three northeast provinces of China.Most of the experimental sites differed markedly in temperature,precipitation, soil fertility and length of growing season.Protein and oil contents were detelmined to study the effects of ecological conditions on soybean chemical composition. The results showed that the average protein and protein + oil contents were higher at the south locations and lower at north locations, but there was no consistent variation for oil content,and there was no significant difference among most of the sites. Oil content of strains was highest when planted in Jiling. Combined analyses of variance indicated that there existed significant genotype,location and G×E interactions effects. F values of G×E interactions for all traits,although significant at 0.01 level of probability, were extremely smaller than those for location and genotype.This suggested that G×E interaction effects were relatively less important than both genotyp and location effects.Phenotypic correlations of the performance of genotypes,on single ehtry -basis,among eight locations were all positive for protein and protein+ oil and most of correlation coefficients were significant at 0.01 level of probability.This indicated that the relative performance of genotypes at each location was similar. In contrast,most of the correlation coefficients for oil content among the eight locations were not significant.Therefore,G×E interactions should be considered in breeding for high oil soybeans,as it may lead to the instability of genotypes and affect the rankings of genotypes when soybeans arc grown in diverse environments.
