We report the results of a search for radio pulsars in five supernova remnants(SNRs)with the FAST telescope.The observations were made using the 19-beam receiver in“snapshot”mode.The integration time for each pointi...We report the results of a search for radio pulsars in five supernova remnants(SNRs)with the FAST telescope.The observations were made using the 19-beam receiver in“snapshot”mode.The integration time for each pointing was 10 min.We discovered a new pulsar,PSR J1845–0306,which has a spin period of 983.6 ms and a dispersion measure of 444.6±2.0 cm^(−3)·pc,in observations of SNR G29.6+0.1.To judge the association between the pulsar and the SNR,further verification is needed.We also re-detected some known pulsars in the data from SNRs G29.6+0.1 and G29.7–0.3.No pulsars were detected in the observations of the other three SNRs.展开更多
Observing and timing a group of millisecond pulsars with high rotational stability enables the direct detection of gravitational waves(GWs).The GW signals can be identified from the spatial correlations encoded in the...Observing and timing a group of millisecond pulsars with high rotational stability enables the direct detection of gravitational waves(GWs).The GW signals can be identified from the spatial correlations encoded in the times-of-arrival of widely spaced pulsar-pairs.The Chinese Pulsar Timing Array(CPTA)is a collaboration aiming at the direct GW detection with observations carried out using Chinese radio telescopes.This short article serves as a“table of contents”for a forthcoming series of papers related to the CPTA Data Release 1(CPTA DR1)which uses observations from the Five-hundred-meter Aperture Spherical radio Telescope.Here,after summarizing the time span and accuracy of CPTA DR1,we report the key results of our statistical inference finding a correlated signal with amplitude logA_(c)=-14.4_(-2.8)^(+1.0)for spectral index in the range ofα∈[-1.8,1.5]assuming a GW background(GWB)induced quadrupolar correlation.The search for the Hellings–Downs(HD)correlation curve is also presented,where some evidence for the HD correlation has been found that a 4.6σstatistical significance is achieved using the discrete frequency method around the frequency of 14 n Hz.We expect that the future International Pulsar Timing Array data analysis and the next CPTA data release will be more sensitive to the n Hz GWB,which could verify the current results.展开更多
Dysfunction of neuronal nitric oxide synthase contributes to neurotoxicity,which triggers cell death in various neuropathological diseases,including epilepsy.Studies have shown that inhibition of neuronal nitric oxide...Dysfunction of neuronal nitric oxide synthase contributes to neurotoxicity,which triggers cell death in various neuropathological diseases,including epilepsy.Studies have shown that inhibition of neuronal nitric oxide synthase activity increases the epilepsy threshold,that is,has an anticonvulsant effect.However,the exact role and potential mechanism of neuronal nitric oxide synthase in seizures are still unclear.In this study,we performed RNA sequencing,functional enrichment analysis,and weighted gene coexpression network analysis of the hippocampus of tremor rats,a rat model of genetic epilepsy.We found damaged hippocampal mitochondria and abnormal succinate dehydrogenase level and Na+-K+-ATPase activity.In addition,we used a pilocarpine-induced N2a cell model to mimic epileptic injury.After application of neuronal nitric oxide synthase inhibitor 7-nitroindazole,changes in malondialdehyde,lactate dehydrogenase and superoxide dismutase,which are associated with oxidative stress,were reversed,and the increase in reactive oxygen species level was reversed by 7-nitroindazole or reactive oxygen species inhibitor N-acetylcysteine.Application of 7-nitroindazole or N-acetylcysteine downregulated the expression of caspase-3 and cytochrome c and reversed the apoptosis of epileptic cells.Furthermore,7-nitroindazole or N-acetylcysteine downregulated the abnormally high expression of NLRP3,gasdermin-D,interleukin-1βand interleukin-18.This indicated that 7-nitroindazole and N-acetylcysteine each reversed epileptic cell death.Taken together,our findings suggest that the neuronal nitric oxide synthase/reactive oxygen species pathway is involved in pyroptosis of epileptic cells,and inhibiting neuronal nitric oxide synthase activity or its induced oxidative stress may play a neuroprotective role in epilepsy.展开更多
The structure of the all-d-metal alloy Ni_(50-x)Co_(x)Mn_(25)V_(25)(x=0–50)is investigated by using theoretical and experimental methods.The first-principles calculations indicate that the most stable structure of th...The structure of the all-d-metal alloy Ni_(50-x)Co_(x)Mn_(25)V_(25)(x=0–50)is investigated by using theoretical and experimental methods.The first-principles calculations indicate that the most stable structure of the Ni_2MnV alloy is face-centered cubic (fcc)type structure with ferrimagnetic state and the equilibrium lattice constant is 3.60A,which is in agreement with the experimental result.It is remarkable that replacing partial Ni with Co can turn the alloy from the fcc structure to the B2-type Heusler structure as Co content x>37 by using the melting spinning method,implying that the d–d hybridization between Co/Mn elements and low-valent elements V stabilizes the Heusler structure.The Curie temperature T_(C) of all-dmetal Heuser alloy Ni_(50-x)Co_(x)Mn_(25)V_(25)(x>37)increases almost linearly with the increase of Co due to that the interaction of Co–Mn is stronger than that of Ni–Mn.A magnetic transition from ferromagnetic state to weak magnetic state accompanying with grinding stress induced transformation from B2 to the dual-phase of B2 and fcc has been observed in these all-d-metal Heusler alloys.This phase transformation and magnetic change provide a guide to overcome the brittleness and make the all-d-metal Heusler alloy interesting in stress and magnetic driving structural transition.展开更多
Combined with two-dimensional(2D)and three-dimensional(3D)finite element analysis and preliminary experimental tests,the effects of size and placement of the electromagnetic shield of the radio-frequency(RF)ion source...Combined with two-dimensional(2D)and three-dimensional(3D)finite element analysis and preliminary experimental tests,the effects of size and placement of the electromagnetic shield of the radio-frequency(RF)ion source with two drivers on plasma parameters and RF power transfer efficiency are analyzed.It is found that the same input direction of the current is better for the RF ion source with multiple drivers.The electromagnetic shield(EMS)should be placed symmetrically around the drivers,which is beneficial for the plasma to distribute uniformly and symmetrically in both drivers.Furthermore,the bigger the EMS shield radius is the better generating a higher electron density.These results will be of guiding significance to the design of electromagnetic shielding for RF ion sources with a multi-driver.展开更多
To alleviate the performance deterioration caused by dynamic stall of a wind turbine airfoil,the flow control by a microsecond-pulsed dielectric barrier discharge(MP-DBD) actuator on the dynamic stall of a periodicall...To alleviate the performance deterioration caused by dynamic stall of a wind turbine airfoil,the flow control by a microsecond-pulsed dielectric barrier discharge(MP-DBD) actuator on the dynamic stall of a periodically pitching NACA0012 airfoil was investigated experimentally.Unsteady pressure measurements with high temporal accuracy were employed in this study,and the unsteady characteristics of the boundary layer were investigated by wavelet packet analysis and the moving root mean square method based on the acquired pressure.The experimental Mach number was 0.2,and the chord-based Reynolds number was 870 000.The dimensionless actuation frequencies F+ were chosen to be 0.5,1,2,and 3,respectively.For the light dynamic regime,the MP-DBD plasma actuator plays the role of suppressing flow separation from the trial edge and accelerating the flow reattachment due to the high-momentum freestream flow being entrained into the boundary layer.Meanwhile,actuation effects were promoted with the increasing dimensionless actuation frequency F+.The control effects of the deep dynamic stall were to delay the onset and reduce the strength of the dynamic stall vortex due to the accumulating vorticity near the leading edge being removed by the induced coherent vortex structures.The laminar fluctuation and Kelvin-Helmholtz(K-H) instabilities of transition and relaminarization were also mitigated by the MP-DBD actuation,and the alleviated K-H rolls led to the delay of the transition onset and earlier laminar reattachment,which improved the hysteresis effect of the dynamic stall.For the controlled cases of F+=2,and F+=3,the laminar fluctuation was replaced by relatively low frequency band disturbances corresponding to the harmonic responses of the MP-DBD actuation frequency.展开更多
A kicker is a critical component for beam injection and accumulation in circular particle accelerators. A ceramic slat kicker plated with a TiN conductive coating was applied in the Beijing Electron Positron Collider ...A kicker is a critical component for beam injection and accumulation in circular particle accelerators. A ceramic slat kicker plated with a TiN conductive coating was applied in the Beijing Electron Positron Collider (BEPCII). However, the ceramic slat kicker has experienced several sudden malfunctions during the operation of the BEPCII in the past. With a reliable kicker structure, a three-metal-strip kicker can substitute the original ceramic slat kicker to maintain the operational stability of the BEPCII. A comparison of the numerical simulation was conducted for three kicker models, demonstrating the comprehensive advantage of the three-metal-strip kicker. Furthermore, impedance bench measurements were conducted on a prototype of a three-metal-strip kicker. The longitudinal beam-coupling impedance was measured using a vector network analyzer via the coaxial wire method. A satisfactory agreement was obtained between the numerical simulations and measurements. Based on the numerical simulation data, the loss factor was 0.01721 V/pC, and the effective impedance was 3.59 mΩ(σ=10 mm).The simulation of the heat deposition on each part of the kicker demonstrated that 84.4%of the parasitic loss of the beam was deposited on the metal strips, and the total heat deposition power on the kicker was between 113.3 and 131.5 W. The obtained heat deposition powers can be considered as a reference for establishing the cooling system.展开更多
Cytokinins(CKs)function in plant development and during stress responses,but their role in drought tolerance in wheat is unknown.In the present study,24 isopentenyltransferase(IPT)genes,encoding ratelimiting enzymes i...Cytokinins(CKs)function in plant development and during stress responses,but their role in drought tolerance in wheat is unknown.In the present study,24 isopentenyltransferase(IPT)genes,encoding ratelimiting enzymes in CK biosynthesis were identified in the wheat genome.The chromosomal locations and structures of the genes,protein properties,and phylogenetic relationships were characterized.ATP/ADP TaIPT genes showed tissue-specific expression.TaIPT2,TaIPT7,and TaIPT8 expression was rapidly induced by 0.5–1 h drought treatments,which decreased to low levels after 2 h drought treatment,as did most other TaIPT genes.TaIPT8-5a/5b/5d triple mutants showed decreased levels of tZtype CK under normal and drought conditions and reduced drought tolerance,which,however,did not manifest as phenotype alterations.By contrast,transgenic wheat plants with drought-induced TaIPT8showed increased drought tolerance.Our study provides a foundation for further investigation of TaIPT genes and novel insights into the role of CKs in the drought response of wheat.展开更多
BACKGROUND Medication misuse or overuse is significantly associated with poor health outcomes.Information regarding the knowledge,cultural beliefs,and behavior about medication safety in the general population is impo...BACKGROUND Medication misuse or overuse is significantly associated with poor health outcomes.Information regarding the knowledge,cultural beliefs,and behavior about medication safety in the general population is important.AIM To conduct a survey on medication habits and explored the potential factors impacting medication safety.METHODS The current survey included adults from 18 districts and counties in Harbin,China.A questionnaire on medication safety was designed based on knowledge,cultural beliefs,and behavior.Both univariate and multivariate analyses were used to explore the factors that impacted medication safety.RESULTS A total of 394 respondents completed the questionnaires on medication safety.The mean scores for knowledge,cultural beliefs,and behavior about medication safety were 59.41±19.33,40.66±9.24,and 60.97±13.69,respectively.The medication knowledge score was affected by age(P=0.044),education(P<0.001),and working status(P=0.015).Moreover,the cultural beliefs score was significantly affected by education(P<0.001).Finally,education(P=0.003)and working status(P=0.011)significantly affected the behavior score.CONCLUSION The knowledge,cultural beliefs,and behavior about medication safety among the general population was moderate.Health education should be provisioned for the elderly,individuals with a low education level,and the unemployed to improve medication safety in Harbin,China.展开更多
Large steerable radio telescopes can rotate in azimuth and elevation,with various upwind postures and complex wind load characteristics.In order to obtain the wind load distribution on the reflector and the wind force...Large steerable radio telescopes can rotate in azimuth and elevation,with various upwind postures and complex wind load characteristics.In order to obtain the wind load distribution on the reflector and the wind force coefficients in different upwind postures,this work took the reflector of the Qi Tai Telescope in Xinjiang as the object.The wind pressure distribution,drag coefficient,side force coefficient,lift coefficient,and total force coefficient of 361 groups of reflectors with different upwind postures were calculated and analyzed by a numerical simulation method.The results show that the force on the reflector when the concave surface faces the wind is significantly greater than when the convex surface faces the wind.The surrogate models for calculating wind force coefficients were established based on the polynomial response surface model(PRSM) and regularized minimumenergy tensor-product spline(RMTS).The adjusted R-squared of RMTS is 0.98,and the root mean square error is below 0.1,which proves RMTS is significantly better than PRSM.Compared with the numerical simulation values,the absolute errors of the drag coefficient,side force coefficient,lift coefficient,and total force coefficient of the reflector with azimuth and elevation of 45° predicted by RMTS are 0.02,0.027,0.032,and 0.046,respectively.The relative errors are 2.42%,2.76%,2.23%,and 2.43%,respectively.It is proved that the RMTS surrogate model is reliable and that the predicted data can fast provide sufficient information for the wind-resistant design of the large steerable radio telescopes.展开更多
We have carried out the Galactic Plane Pulsar Snapshot(GPPS)survey by using the Five-hundred-meter Aperture Spherical radio Telescope(FAST),the most sensitive systematic pulsar survey in the Galactic plane.In addition...We have carried out the Galactic Plane Pulsar Snapshot(GPPS)survey by using the Five-hundred-meter Aperture Spherical radio Telescope(FAST),the most sensitive systematic pulsar survey in the Galactic plane.In addition to more than 500 pulsars already discovered through normal periodical search,we report here the discovery of 76 new transient radio sources with sporadic strong pulses,detected by using the newly developed module for a sensitive single-pulse search.Their small DM values suggest that they all are Galactic rotating radio transients(RRATs).They show different properties in the follow-up observations.More radio pulses have been detected from 26 transient radio sources but no periods can be found due to a limited small number of pulses from all FAST observations.The followup observations show that 16 transient sources are newly identified as being the prototypes of RRATs with a period already determined from more detected sporadic pulses,and 10 sources are extremely nulling pulsars,and 24 sources are weak pulsars with sparse strong pulses.On the other hand,48 previously known RRATs have been detected by the FAST,either during verification observations for the GPPS survey or through targeted observations of applied normal FAST projects.Except for one RRAT with four pulses detected in a session of 5-minute observation and four RRATs with only one pulse detected in a session,sensitive FAST observations reveal that 43 RRATs are just generally weak pulsars with sporadic strong pulses or simply very nulling pulsars,so that the previously known RRATs always have an extreme emission state together with a normal hardly detectable weak emission state.This is echoed by the two normal pulsars J1938+2213 and J1946+1449 with occasional brightening pulses.Though strong pulses of RRATs are very outstanding in the energy distribution,their polarization angle variations follow the polarization angle curve of the averaged normal pulse profile,suggesting that the predominant sparse pulses of RRATs are emitted in the same region with the same geometry as normal weak pulsars.展开更多
This paper presents a modified Stewart platform that enables precise adjustment of the sub-reflector for the Qitai Radio Telescope(QTT).QTT demands a parallel platform capable of carrying a heavy load(≥4000 kg),and m...This paper presents a modified Stewart platform that enables precise adjustment of the sub-reflector for the Qitai Radio Telescope(QTT).QTT demands a parallel platform capable of carrying a heavy load(≥4000 kg),and moving in elevation from 5°to 88°together with the primary reflector while precisely adjusting the sub-reflector’s position in five degrees of freedom.To meet these requirements,a modified Stewart platform with two separated actuators is proposed,and the comprehensive performance is analyzed and optimized for practical application.The performance of the modified platform is compared to that of the traditional Stewart platform,and the results demonstrate that the modified platform has superior load-bearing capacity and stiffness over the entire elevation angle with more uniform actuator load and stiffness distributions.These results indicate that the modified Stewart platform is well-suited for practical application in QTT.展开更多
Studies have shown that the m6A reader primarily affects genes expression by participating in the regulation of mRNA localization,splicing,degradation,translation,and other metabolic processes.Previously,we discovered...Studies have shown that the m6A reader primarily affects genes expression by participating in the regulation of mRNA localization,splicing,degradation,translation,and other metabolic processes.Previously,we discovered that the apple(Malus domestica)m6A reader MhYTP2 bound with and destabilized m6A-modified MdMLO19 mRNA.In addition,it enhanced the translation efficiency of m6A-modified mRNA of MdGDH1L,encoding a glutamate dehydrogenase,which confers resistance to powdery mildew.In this study,we report the function of MhYTP2 in the regulation of resistance to low nitrogen(N).The overexpression of MhYTP2 enhances the resistance of apple to low N.We show that MhYTP2 binds with and stabilizes the mRNAs of MdALN,which participates in the allantoin catabolic process and cellular response to N starvation in apple;MdPIDL,which participates in root hair elongation;MdTTG1,which is involved in the differentiation process of trichomes;and MdATG8A,which is a core participant in the regulation of autophagy.In addition,MhYTP2 accelerates the degradation of MdRHD3 mRNA,which regulates root development.RNA immunoprecipitation-seq and electrophoretic mobility shift assays show that the mRNAs of MdALN,MdATG8A,MdPIDL,MdTTG1,and MdRHD3 are the direct targets of MhYTP2.Overexpressing or knocking down the above genes in MhYTP2 overexpressing plants dismisses the function of MhYTP2 under low N,suggesting the role of MhYTP2 is dependent on those genes.Together,these results demonstrate that MhYTP2 enhances the resistance of apple to N deficiency by affecting the stability of the bound mRNAs.展开更多
BACKGROUND Microalbuminuria is an early and informative marker of diabetic nephropathy.Our study found that microalbuminuria developed in patients with newly diagnosed type 2 diabetes mellitus(T2DM).AIM To investigate...BACKGROUND Microalbuminuria is an early and informative marker of diabetic nephropathy.Our study found that microalbuminuria developed in patients with newly diagnosed type 2 diabetes mellitus(T2DM).AIM To investigate the association between glucagon-like peptide 1(GLP-1)and microalbuminuria in newly diagnosed T2DM patients.METHODS In total,760 patients were recruited for this cross-sectional study.The GLP-1 levels during a standard meal test and urinary albumin-creatinine ratio(UACR)were determined.RESULTS Patients with microalbuminuria exhibited lower GLP-1 levels at 30 min and 120 min during a standard meal test than patients with normal albuminuria(30 min GLP-1,16.7±13.3 pmol vs 19.9±15.6 pmol,P=0.007;120 min GLP-1,16.0±14.1 pmol vs 18.4±13.8 pmol,P=0.037).The corresponding area under the curve for active GLP-1(AUCGLP-1)was also lower in microalbuminuria patients(2257,1585 to 3506 vs 2896,1763 to 4726,pmol×min,P=0.003).Postprandial GLP-1 levels at 30 min and 120 min and AUCGLP-1 were negatively correlated with the UACR(r=0.159,r=0.132,r=0.206,respectively,P<0.001).The prevalence of microalbuminuria in patients with newly diagnosed T2DM was 21.7%,which decreased with increasing quartiles of AUCGLP-1 levels(27.4%,25.3%,18.9%and 15.8%).After logistic regression analysis adjusted for sex,age,hemoglobin A1c,body mass index,systolic blood pressure,estimated glomerular filtration rate,homeostasis model assessment of insulin resistance,AUC_(glucose)and AUC_(glucagon)patients in quartile 4 of the AUCGLP-1 presented a lower risk of microalbuminuria compared with the patients in quartile 1(odds ratio=0.547,95%confidence interval:0.325-0.920,P=0.01).A consistent association was also found between 30 min GLP-1 or 120 min GLP-1 and microalbuminuria.CONCLUSION Postprandial GLP-1 levels were independently associated with microalbuminuria in newly diagnosed Chinese T2DM patients.展开更多
Fast radio bursts(FRBs) are highly dispersed millisecond-duration radio bursts,[1,2]of which the physical origin is still not fully understood. FRB 20201124A is one of the most actively repeating FRBs. In this paper, ...Fast radio bursts(FRBs) are highly dispersed millisecond-duration radio bursts,[1,2]of which the physical origin is still not fully understood. FRB 20201124A is one of the most actively repeating FRBs. In this paper, we present the collection of 1863 burst dynamic spectra of FRB 20201124A measured with the Five-hundred-meter Aperture Spherical radio Telescope(FAST). The current collection, taken from the observation during the FRB active phase from April to June 2021, is the largest burst sample detected for any FRB so far. The standard PSRFITs format is adopted, including dynamic spectra of the burst, and the time information of the dynamic spectra, in addition, mask files help readers to identify the pulse positions are also provided. The dataset is available in Science Data Bank, with the link https://www.doi.org/10.57760/sciencedb.j00113.00076.展开更多
[Objectives]The paper was to clone and analyze bioinformatics of vscN gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the vscN gene sequence of V.alginolyticus HY9901.The full ...[Objectives]The paper was to clone and analyze bioinformatics of vscN gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the vscN gene sequence of V.alginolyticus HY9901.The full length of vscN gene was amplified by PCR and bioinformatics analysis was performed.[Results]The vscN gene was 1323 bp in total,encoding 440 amino acids,with the theoretical molecular weight of 47.86 kD and the theoretical pI value of 5.89.The online prediction showed that there was no signal peptide and no transmembrane region in VscN.The amino acid sequence had 10 N-myristoylation sites,8 phosphorylation sites(2 protein kinase C phosphorylation sites,6 casein kinase II phosphorylation sites),1 amidation site,11 microbody C-terminal target signal sites,1 ATP/GTP binding site motif A(P ring),and 1 ATPaseαandβsubunit specific site.Homology analysis showed that the VscN protein of V.alginolyticus had high homology with that of V.antiquarius,with a similarity of 95.14%.Phylogenetic tree analysis showed that the VscN of V.alginolyticus was clustered into the same subgroup as that of V.diabolicus and V.antiquarius.Functional domain analysis of VscN protein showed that it had Pfam and AAA domains,and involved in the regulation of bacterial virulence.The three-dimensional structure model of VscN simulated by SWISS-MODEL software was similar to the structure of flagellate-specific ATPase FliH-FliI complex.[Conclusions]The results lay a foundation for further study on the regulatory mechanism of VscN protein on bacterial virulence.展开更多
[Objectives]To clone and analyze the vscB gene of Vibrio alginolyticus HY9901 by bioinformatics.[Methods]A pair of specific primers were designed according to the vscB gene sequence of Vibrio alginolyticus HY9901.The ...[Objectives]To clone and analyze the vscB gene of Vibrio alginolyticus HY9901 by bioinformatics.[Methods]A pair of specific primers were designed according to the vscB gene sequence of Vibrio alginolyticus HY9901.The full length of the primers was cloned by PCR and analyzed by bioinformatics.[Results]The vscB gene was 429 bp long,encoding 142 amino acids,with a theoretical molecular weight of 16.4 kDa and a pI value of 5.48.Amino acid sequence analysis of VscB showed that VscB was not a secretory protein,without signal peptide and transmembrane region,and there were protein kinase C phosphorylation site and casein kinase II phosphorylation site in the sequence.Homologous comparison of amino acid sequences showed that VscB of V.alginolyticus had the highest protein similarity with Vibrio Parahaemolyticus,reaching 91%.Phylogenetic tree analysis showed that the corresponding proteins of V.alginolyticus VscB,Vibrio Parahaemolyticus and Vibrio diabolicus were clustered in the same subfamily.Functional domain analysis showed that it had CesT family domain.Tertiary structure prediction showed that there were 3α-helices and 5β-turns in VscB protein.[Conclusions]This study provided a theoretical basis for further study on the function of chaperone of V.alginolyticus.展开更多
[Objectives]To amplify the h-ns gene of Vibrio alginolyticus and analyze it by bioinformatics.[Methods]According to the h-ns gene sequence of V.alginolyticus HY9901,a pair of specific primers were designed and amplifi...[Objectives]To amplify the h-ns gene of Vibrio alginolyticus and analyze it by bioinformatics.[Methods]According to the h-ns gene sequence of V.alginolyticus HY9901,a pair of specific primers were designed and amplified by PCR.[Results]The h-ns gene was 408 bp in length and 135 amino acids were encoded.The predicted theoretical protein molecular weight was about 14.98 kD,and the isoelectric point was 4.99.Protein subcellular localization,SignalP 5.0,TMHMM Server 2.0 and SoftBerry-Psite predictions showed that H-NS was located outside the cell membrane,and the protein was unstable and hydrophobic.There was no signal peptide cleavage site,no transmembrane region and no KEGG metabolic pathway.The amino acid sequence contained three phosphorylation sites,one N-terminal myristoylation site and three microsomal C-terminal target signal sites.Using MEGA 5.0,H-NS phylogenetic tree was constructed by ortho-connection method.The results showed that H-NS of V.alginolyticus was closer to H-NS of Vibrio diabolicus.Using SWISS-MODEL,the three-dimensional structure model of H-NS subunit was simulated,which was similar to the crystal structure of Salmonella typhimurium H-NS1-83.[Conclusions]This study lays a foundation for exploring the regulation mechanism of V.alginolyticus H-NS protein on bacterial virulence in the future.展开更多
[Objectives]The paper was to clone and analyze bioinformatics of ndk gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the ndk gene sequence of V.alginolyticus HY9901.The full le...[Objectives]The paper was to clone and analyze bioinformatics of ndk gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the ndk gene sequence of V.alginolyticus HY9901.The full length of ndk gene was amplified by PCR and bioinformatics analysis was performed.MEGA 5.0 software was used to construct NDK phylogenetic tree by neighbor-joining method.SWISS-MODEL program was used to obtain the three-dimensional structural model of single subunit from NDK protein.[Results]The ndk gene,molecular structural formula C702H1094N192O214S7,was 426 bp in total,encoding 141 amino acids,with the theoretical molecular weight of 15.87199 kD and the theoretical pI value of 5.13.The prediction results of protein subcellular localization,SignalP 5.0,TMHMM Server 2.0 and SoftBerry-Psite showed that NDK mainly existed in the cytoplasm,and the protein was unstable and hydrophobic.There was neither signal peptide cleavage site,nor transmembrane region and KEGG metabolic pathway.The amino acid sequence had two protein kinase C phosphorylation sites,a casein kinaseⅡphosphorylation site,a N-myristoylation site,three microbody C-terminal target signal sites,and a nucleoside diphosphate kinase active site.Homology analysis showed that the NDK of V.alginolyticus had high homology with that of V.diabolicus,with a similarity of 98.58%.Analysis of the structural functional domain revealed that the protein had one NDK structural functional domain.The prediction results of secondary structure showed that theα-helix,random coil,β-sheet and extended strand accounted for 53.19%,28.37%,7.09%and 11.35%,respectively.Analysis of NDK protein via STRING database demonstrated that the proteins interacting with NDK protein were NrdA,NrdB,GmK,CmK,TmK,PyrG,PyrH,RelA,FolE and SpoT.[Conclusions]The study plays a positive role in the prevention and control of vibriosis and the improvement of the current aquaculture environment.展开更多
The East Asian Very Long Baseline Interferometry(VLBI) Network(EAVN) is a rapidly evolving international VLBI array that is currently promoted under joint efforts among China,Japan and Korea.EAVN aims at forming a joi...The East Asian Very Long Baseline Interferometry(VLBI) Network(EAVN) is a rapidly evolving international VLBI array that is currently promoted under joint efforts among China,Japan and Korea.EAVN aims at forming a joint VLBI Network by combining a large number of radio telescopes distributed over East Asian regions.After the combination of the Korean VLBI Network(KVN) and the VLBI Exploration of Radio Astrometry(VERA) into Ka VA,further expansion with the joint array in East Asia is actively promoted.Here we report the first imaging results(at 22 and 43 GHz) of bright radio sources obtained with Ka VA connected to Tianma 65-m and Nanshan 26-m Radio Telescopes in China.To test the EAVN imaging performance for different sources,we observed four active galactic nuclei(AGN) having different brightness and morphology.As a result,we confirmed that the Tianma 65-m Radio Telescope(TMRT) significantly enhances the overall array sensitivity,a factor of 4 improvement in baseline sensitivity and 2 in image dynamic range compared to the case of Ka VA only.The addition of the Nanshan 26-m Radio Telescope(NSRT) further doubled the east-west angular resolution.With the resulting high-dynamic-range,high-resolution images with EAVN(Ka VA+TMRT+NSRT),various fine-scale structures in our targets,such as the counter-jet in M87,a kink-like morphology of the 3 C 273 jet and the weak emission in other sources are successfully detected.This demonstrates the powerful capability of EAVN to study AGN jets and to achieve other science goals in general.Ongoing expansion of EAVN will further enhance the angular resolution,detection sensitivity and frequency coverage of the network.展开更多
基金supported by the National SKA Program of China (Grant No. 2020SKA0120200)the National Natural Science Foundation of China (Grant Nos. 12041303, 12273100, 12041304, and 12288102)+5 种基金the National Key R&D Program of China (Grant No. 2022YFC2205201)the West Light Foundation of the Chinese Academy of Sciences (Grant No. WLFC 2021-XBQNXZ-027)the Major Science and Technology Program of Xinjiang Uygur Autonomous Region (Grant No. 2022A03013-4)the Natural Science Foundation of Xinjiiang Uygur Autonomous Region (Grant No. 2022D01D85)the open program of the Key Laboratory of Xinjiang Uygur Autonomous Region (Grant No. 2020D04049)partly supported by the Operation, Maintenance and Upgrading Fund for Astronomical Telescopes and Facility Instruments, budgeted from the Ministry of Finance of China and administrated by the CAS
文摘We report the results of a search for radio pulsars in five supernova remnants(SNRs)with the FAST telescope.The observations were made using the 19-beam receiver in“snapshot”mode.The integration time for each pointing was 10 min.We discovered a new pulsar,PSR J1845–0306,which has a spin period of 983.6 ms and a dispersion measure of 444.6±2.0 cm^(−3)·pc,in observations of SNR G29.6+0.1.To judge the association between the pulsar and the SNR,further verification is needed.We also re-detected some known pulsars in the data from SNRs G29.6+0.1 and G29.7–0.3.No pulsars were detected in the observations of the other three SNRs.
基金supported by the National SKA Program of China(2020SKA0120100)the National Natural Science Foundation of China(Nos.12041303 and 12250410246)+1 种基金the CAS-MPG LEGACY projectfunding from the Max-Planck Partner Group。
文摘Observing and timing a group of millisecond pulsars with high rotational stability enables the direct detection of gravitational waves(GWs).The GW signals can be identified from the spatial correlations encoded in the times-of-arrival of widely spaced pulsar-pairs.The Chinese Pulsar Timing Array(CPTA)is a collaboration aiming at the direct GW detection with observations carried out using Chinese radio telescopes.This short article serves as a“table of contents”for a forthcoming series of papers related to the CPTA Data Release 1(CPTA DR1)which uses observations from the Five-hundred-meter Aperture Spherical radio Telescope.Here,after summarizing the time span and accuracy of CPTA DR1,we report the key results of our statistical inference finding a correlated signal with amplitude logA_(c)=-14.4_(-2.8)^(+1.0)for spectral index in the range ofα∈[-1.8,1.5]assuming a GW background(GWB)induced quadrupolar correlation.The search for the Hellings–Downs(HD)correlation curve is also presented,where some evidence for the HD correlation has been found that a 4.6σstatistical significance is achieved using the discrete frequency method around the frequency of 14 n Hz.We expect that the future International Pulsar Timing Array data analysis and the next CPTA data release will be more sensitive to the n Hz GWB,which could verify the current results.
基金supported by the Natural Science Foundation of ChinaNos.81971212 (to FG)+7 种基金81601129 (to XXX)the Open Fund of the Key Laboratory of Medical ElectrophysiologyMinistry of Education&Medical Electrophysiological Key Laboratory of Sichuan ProvinceInstitute of Cardiovascular ResearchSouthwest Medical UniversityNo.KeyME-2018-07 (to FG)Liaoning Province Xingliao Talent Program ProjectNo.XLYC1907164 (to FG)
文摘Dysfunction of neuronal nitric oxide synthase contributes to neurotoxicity,which triggers cell death in various neuropathological diseases,including epilepsy.Studies have shown that inhibition of neuronal nitric oxide synthase activity increases the epilepsy threshold,that is,has an anticonvulsant effect.However,the exact role and potential mechanism of neuronal nitric oxide synthase in seizures are still unclear.In this study,we performed RNA sequencing,functional enrichment analysis,and weighted gene coexpression network analysis of the hippocampus of tremor rats,a rat model of genetic epilepsy.We found damaged hippocampal mitochondria and abnormal succinate dehydrogenase level and Na+-K+-ATPase activity.In addition,we used a pilocarpine-induced N2a cell model to mimic epileptic injury.After application of neuronal nitric oxide synthase inhibitor 7-nitroindazole,changes in malondialdehyde,lactate dehydrogenase and superoxide dismutase,which are associated with oxidative stress,were reversed,and the increase in reactive oxygen species level was reversed by 7-nitroindazole or reactive oxygen species inhibitor N-acetylcysteine.Application of 7-nitroindazole or N-acetylcysteine downregulated the expression of caspase-3 and cytochrome c and reversed the apoptosis of epileptic cells.Furthermore,7-nitroindazole or N-acetylcysteine downregulated the abnormally high expression of NLRP3,gasdermin-D,interleukin-1βand interleukin-18.This indicated that 7-nitroindazole and N-acetylcysteine each reversed epileptic cell death.Taken together,our findings suggest that the neuronal nitric oxide synthase/reactive oxygen species pathway is involved in pyroptosis of epileptic cells,and inhibiting neuronal nitric oxide synthase activity or its induced oxidative stress may play a neuroprotective role in epilepsy.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.51671024 and 52088101)State Key Lab of Advanced Metals and Materials(Grant No.2019Z12)the Fundamental Research Funds for the Central Universities(Grant No.FRF-BD-20-12A)。
文摘The structure of the all-d-metal alloy Ni_(50-x)Co_(x)Mn_(25)V_(25)(x=0–50)is investigated by using theoretical and experimental methods.The first-principles calculations indicate that the most stable structure of the Ni_2MnV alloy is face-centered cubic (fcc)type structure with ferrimagnetic state and the equilibrium lattice constant is 3.60A,which is in agreement with the experimental result.It is remarkable that replacing partial Ni with Co can turn the alloy from the fcc structure to the B2-type Heusler structure as Co content x>37 by using the melting spinning method,implying that the d–d hybridization between Co/Mn elements and low-valent elements V stabilizes the Heusler structure.The Curie temperature T_(C) of all-dmetal Heuser alloy Ni_(50-x)Co_(x)Mn_(25)V_(25)(x>37)increases almost linearly with the increase of Co due to that the interaction of Co–Mn is stronger than that of Ni–Mn.A magnetic transition from ferromagnetic state to weak magnetic state accompanying with grinding stress induced transformation from B2 to the dual-phase of B2 and fcc has been observed in these all-d-metal Heusler alloys.This phase transformation and magnetic change provide a guide to overcome the brittleness and make the all-d-metal Heusler alloy interesting in stress and magnetic driving structural transition.
基金supported by the Comprehensive Research Facility for Fusion Technology Program of China(No.2018-000052-73-01-001228)National Natural Science Foundation of China(No.11975263)the National Key R&D Program of China(No.2017YFE0300101)。
文摘Combined with two-dimensional(2D)and three-dimensional(3D)finite element analysis and preliminary experimental tests,the effects of size and placement of the electromagnetic shield of the radio-frequency(RF)ion source with two drivers on plasma parameters and RF power transfer efficiency are analyzed.It is found that the same input direction of the current is better for the RF ion source with multiple drivers.The electromagnetic shield(EMS)should be placed symmetrically around the drivers,which is beneficial for the plasma to distribute uniformly and symmetrically in both drivers.Furthermore,the bigger the EMS shield radius is the better generating a higher electron density.These results will be of guiding significance to the design of electromagnetic shielding for RF ion sources with a multi-driver.
基金supported by National Natural Science Foundation of China(Nos.12172299 and 1190021162)。
文摘To alleviate the performance deterioration caused by dynamic stall of a wind turbine airfoil,the flow control by a microsecond-pulsed dielectric barrier discharge(MP-DBD) actuator on the dynamic stall of a periodically pitching NACA0012 airfoil was investigated experimentally.Unsteady pressure measurements with high temporal accuracy were employed in this study,and the unsteady characteristics of the boundary layer were investigated by wavelet packet analysis and the moving root mean square method based on the acquired pressure.The experimental Mach number was 0.2,and the chord-based Reynolds number was 870 000.The dimensionless actuation frequencies F+ were chosen to be 0.5,1,2,and 3,respectively.For the light dynamic regime,the MP-DBD plasma actuator plays the role of suppressing flow separation from the trial edge and accelerating the flow reattachment due to the high-momentum freestream flow being entrained into the boundary layer.Meanwhile,actuation effects were promoted with the increasing dimensionless actuation frequency F+.The control effects of the deep dynamic stall were to delay the onset and reduce the strength of the dynamic stall vortex due to the accumulating vorticity near the leading edge being removed by the induced coherent vortex structures.The laminar fluctuation and Kelvin-Helmholtz(K-H) instabilities of transition and relaminarization were also mitigated by the MP-DBD actuation,and the alleviated K-H rolls led to the delay of the transition onset and earlier laminar reattachment,which improved the hysteresis effect of the dynamic stall.For the controlled cases of F+=2,and F+=3,the laminar fluctuation was replaced by relatively low frequency band disturbances corresponding to the harmonic responses of the MP-DBD actuation frequency.
基金supported by the National Natural Science Foundation of China (Nos.Y8113C005C and U1832132)。
文摘A kicker is a critical component for beam injection and accumulation in circular particle accelerators. A ceramic slat kicker plated with a TiN conductive coating was applied in the Beijing Electron Positron Collider (BEPCII). However, the ceramic slat kicker has experienced several sudden malfunctions during the operation of the BEPCII in the past. With a reliable kicker structure, a three-metal-strip kicker can substitute the original ceramic slat kicker to maintain the operational stability of the BEPCII. A comparison of the numerical simulation was conducted for three kicker models, demonstrating the comprehensive advantage of the three-metal-strip kicker. Furthermore, impedance bench measurements were conducted on a prototype of a three-metal-strip kicker. The longitudinal beam-coupling impedance was measured using a vector network analyzer via the coaxial wire method. A satisfactory agreement was obtained between the numerical simulations and measurements. Based on the numerical simulation data, the loss factor was 0.01721 V/pC, and the effective impedance was 3.59 mΩ(σ=10 mm).The simulation of the heat deposition on each part of the kicker demonstrated that 84.4%of the parasitic loss of the beam was deposited on the metal strips, and the total heat deposition power on the kicker was between 113.3 and 131.5 W. The obtained heat deposition powers can be considered as a reference for establishing the cooling system.
基金financially supported by the National Key Research and Development Program of China(2020YFE0202300)the Agricultural Science and Technology Innovation Program(CAASZDRW202109 and CAAS-ZDRW202002)。
文摘Cytokinins(CKs)function in plant development and during stress responses,but their role in drought tolerance in wheat is unknown.In the present study,24 isopentenyltransferase(IPT)genes,encoding ratelimiting enzymes in CK biosynthesis were identified in the wheat genome.The chromosomal locations and structures of the genes,protein properties,and phylogenetic relationships were characterized.ATP/ADP TaIPT genes showed tissue-specific expression.TaIPT2,TaIPT7,and TaIPT8 expression was rapidly induced by 0.5–1 h drought treatments,which decreased to low levels after 2 h drought treatment,as did most other TaIPT genes.TaIPT8-5a/5b/5d triple mutants showed decreased levels of tZtype CK under normal and drought conditions and reduced drought tolerance,which,however,did not manifest as phenotype alterations.By contrast,transgenic wheat plants with drought-induced TaIPT8showed increased drought tolerance.Our study provides a foundation for further investigation of TaIPT genes and novel insights into the role of CKs in the drought response of wheat.
基金Supported by 2021 Science Popularization Research Project of National Medical Information Network,Chinese Pharmaceutical Association,No.CMEI2021KPYJ00101。
文摘BACKGROUND Medication misuse or overuse is significantly associated with poor health outcomes.Information regarding the knowledge,cultural beliefs,and behavior about medication safety in the general population is important.AIM To conduct a survey on medication habits and explored the potential factors impacting medication safety.METHODS The current survey included adults from 18 districts and counties in Harbin,China.A questionnaire on medication safety was designed based on knowledge,cultural beliefs,and behavior.Both univariate and multivariate analyses were used to explore the factors that impacted medication safety.RESULTS A total of 394 respondents completed the questionnaires on medication safety.The mean scores for knowledge,cultural beliefs,and behavior about medication safety were 59.41±19.33,40.66±9.24,and 60.97±13.69,respectively.The medication knowledge score was affected by age(P=0.044),education(P<0.001),and working status(P=0.015).Moreover,the cultural beliefs score was significantly affected by education(P<0.001).Finally,education(P=0.003)and working status(P=0.011)significantly affected the behavior score.CONCLUSION The knowledge,cultural beliefs,and behavior about medication safety among the general population was moderate.Health education should be provisioned for the elderly,individuals with a low education level,and the unemployed to improve medication safety in Harbin,China.
基金supported by the National Key Research and Development Program of China(grant No.2021YFC2203600)the National Natural Science Foundation of China(Grant Nos.52275268 and 51975447)+2 种基金the National Defense Basic Scientific Research Program of China(Grant No.JCKY2021210B007)Wuhu and Xidian University Special Fund for Industry-University-Research Cooperation(Grant No.XWYCXY-012021012)Youth Innovation Team of Shaanxi Universities(Grant No.201926)。
文摘Large steerable radio telescopes can rotate in azimuth and elevation,with various upwind postures and complex wind load characteristics.In order to obtain the wind load distribution on the reflector and the wind force coefficients in different upwind postures,this work took the reflector of the Qi Tai Telescope in Xinjiang as the object.The wind pressure distribution,drag coefficient,side force coefficient,lift coefficient,and total force coefficient of 361 groups of reflectors with different upwind postures were calculated and analyzed by a numerical simulation method.The results show that the force on the reflector when the concave surface faces the wind is significantly greater than when the convex surface faces the wind.The surrogate models for calculating wind force coefficients were established based on the polynomial response surface model(PRSM) and regularized minimumenergy tensor-product spline(RMTS).The adjusted R-squared of RMTS is 0.98,and the root mean square error is below 0.1,which proves RMTS is significantly better than PRSM.Compared with the numerical simulation values,the absolute errors of the drag coefficient,side force coefficient,lift coefficient,and total force coefficient of the reflector with azimuth and elevation of 45° predicted by RMTS are 0.02,0.027,0.032,and 0.046,respectively.The relative errors are 2.42%,2.76%,2.23%,and 2.43%,respectively.It is proved that the RMTS surrogate model is reliable and that the predicted data can fast provide sufficient information for the wind-resistant design of the large steerable radio telescopes.
基金This project,as one of five key projects,is being carried out by using FAST,a Chinese national mega-science facility built and operated by the National Astronomical Observatories,Chinese Academy of Sciencessupported by the National Natural Science Foundation of China(NSFC,Nos.11988101 and 11833009)+5 种基金the Key Research Program of the Chinese Academy of Sciences(grant No.QYZDJ-SSWSLH021)supported by the Cultivation Project for the FAST scientific Payoff and Research Achievement of CAMS-CASsupported by NSFC No.12133004,partially supported by NSFC No.U1731120partially supported by the NSFC No.11873058,partially supported by NSFC No.U2031115partially supported by the National SKA program of China No.2020SKA0120200partially supported by the Guangzhou Science and Technology Project No.202102010466。
文摘We have carried out the Galactic Plane Pulsar Snapshot(GPPS)survey by using the Five-hundred-meter Aperture Spherical radio Telescope(FAST),the most sensitive systematic pulsar survey in the Galactic plane.In addition to more than 500 pulsars already discovered through normal periodical search,we report here the discovery of 76 new transient radio sources with sporadic strong pulses,detected by using the newly developed module for a sensitive single-pulse search.Their small DM values suggest that they all are Galactic rotating radio transients(RRATs).They show different properties in the follow-up observations.More radio pulses have been detected from 26 transient radio sources but no periods can be found due to a limited small number of pulses from all FAST observations.The followup observations show that 16 transient sources are newly identified as being the prototypes of RRATs with a period already determined from more detected sporadic pulses,and 10 sources are extremely nulling pulsars,and 24 sources are weak pulsars with sparse strong pulses.On the other hand,48 previously known RRATs have been detected by the FAST,either during verification observations for the GPPS survey or through targeted observations of applied normal FAST projects.Except for one RRAT with four pulses detected in a session of 5-minute observation and four RRATs with only one pulse detected in a session,sensitive FAST observations reveal that 43 RRATs are just generally weak pulsars with sporadic strong pulses or simply very nulling pulsars,so that the previously known RRATs always have an extreme emission state together with a normal hardly detectable weak emission state.This is echoed by the two normal pulsars J1938+2213 and J1946+1449 with occasional brightening pulses.Though strong pulses of RRATs are very outstanding in the energy distribution,their polarization angle variations follow the polarization angle curve of the averaged normal pulse profile,suggesting that the predominant sparse pulses of RRATs are emitted in the same region with the same geometry as normal weak pulsars.
基金the National Natural Science Foundation of China(NSFC,grant No.12203095)the Youth Innovation Promotion Association,CAS(No.Y202019)+2 种基金Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2022D01B220)Tianchi Doctoral Program 2021the Scientific Instrument Developing Project of the Chinese Academy of Sciences(No.PTYQ2022YZZD01).
文摘This paper presents a modified Stewart platform that enables precise adjustment of the sub-reflector for the Qitai Radio Telescope(QTT).QTT demands a parallel platform capable of carrying a heavy load(≥4000 kg),and moving in elevation from 5°to 88°together with the primary reflector while precisely adjusting the sub-reflector’s position in five degrees of freedom.To meet these requirements,a modified Stewart platform with two separated actuators is proposed,and the comprehensive performance is analyzed and optimized for practical application.The performance of the modified platform is compared to that of the traditional Stewart platform,and the results demonstrate that the modified platform has superior load-bearing capacity and stiffness over the entire elevation angle with more uniform actuator load and stiffness distributions.These results indicate that the modified Stewart platform is well-suited for practical application in QTT.
基金This work was financially supported grants from the National Natural Science Foundation of China(31972391,32172529)the China Agriculture Research System of MOF and MARA(CARS-27)the Special Funds for Major Scientific and Technological Innovation from Shaanxi Province(2020zdzx03-01-01).
文摘Studies have shown that the m6A reader primarily affects genes expression by participating in the regulation of mRNA localization,splicing,degradation,translation,and other metabolic processes.Previously,we discovered that the apple(Malus domestica)m6A reader MhYTP2 bound with and destabilized m6A-modified MdMLO19 mRNA.In addition,it enhanced the translation efficiency of m6A-modified mRNA of MdGDH1L,encoding a glutamate dehydrogenase,which confers resistance to powdery mildew.In this study,we report the function of MhYTP2 in the regulation of resistance to low nitrogen(N).The overexpression of MhYTP2 enhances the resistance of apple to low N.We show that MhYTP2 binds with and stabilizes the mRNAs of MdALN,which participates in the allantoin catabolic process and cellular response to N starvation in apple;MdPIDL,which participates in root hair elongation;MdTTG1,which is involved in the differentiation process of trichomes;and MdATG8A,which is a core participant in the regulation of autophagy.In addition,MhYTP2 accelerates the degradation of MdRHD3 mRNA,which regulates root development.RNA immunoprecipitation-seq and electrophoretic mobility shift assays show that the mRNAs of MdALN,MdATG8A,MdPIDL,MdTTG1,and MdRHD3 are the direct targets of MhYTP2.Overexpressing or knocking down the above genes in MhYTP2 overexpressing plants dismisses the function of MhYTP2 under low N,suggesting the role of MhYTP2 is dependent on those genes.Together,these results demonstrate that MhYTP2 enhances the resistance of apple to N deficiency by affecting the stability of the bound mRNAs.
文摘BACKGROUND Microalbuminuria is an early and informative marker of diabetic nephropathy.Our study found that microalbuminuria developed in patients with newly diagnosed type 2 diabetes mellitus(T2DM).AIM To investigate the association between glucagon-like peptide 1(GLP-1)and microalbuminuria in newly diagnosed T2DM patients.METHODS In total,760 patients were recruited for this cross-sectional study.The GLP-1 levels during a standard meal test and urinary albumin-creatinine ratio(UACR)were determined.RESULTS Patients with microalbuminuria exhibited lower GLP-1 levels at 30 min and 120 min during a standard meal test than patients with normal albuminuria(30 min GLP-1,16.7±13.3 pmol vs 19.9±15.6 pmol,P=0.007;120 min GLP-1,16.0±14.1 pmol vs 18.4±13.8 pmol,P=0.037).The corresponding area under the curve for active GLP-1(AUCGLP-1)was also lower in microalbuminuria patients(2257,1585 to 3506 vs 2896,1763 to 4726,pmol×min,P=0.003).Postprandial GLP-1 levels at 30 min and 120 min and AUCGLP-1 were negatively correlated with the UACR(r=0.159,r=0.132,r=0.206,respectively,P<0.001).The prevalence of microalbuminuria in patients with newly diagnosed T2DM was 21.7%,which decreased with increasing quartiles of AUCGLP-1 levels(27.4%,25.3%,18.9%and 15.8%).After logistic regression analysis adjusted for sex,age,hemoglobin A1c,body mass index,systolic blood pressure,estimated glomerular filtration rate,homeostasis model assessment of insulin resistance,AUC_(glucose)and AUC_(glucagon)patients in quartile 4 of the AUCGLP-1 presented a lower risk of microalbuminuria compared with the patients in quartile 1(odds ratio=0.547,95%confidence interval:0.325-0.920,P=0.01).A consistent association was also found between 30 min GLP-1 or 120 min GLP-1 and microalbuminuria.CONCLUSION Postprandial GLP-1 levels were independently associated with microalbuminuria in newly diagnosed Chinese T2DM patients.
基金supported by the National SKA Program of China (Grant Nos. 2020SKA0120100 and 2020SKA0120200)the National Natural Science Foundation of China (Grant Nos. 12041304, 11873067, 11988101, 12041303, 11725313, 11725314, 11833003, 12003028, 12041306, 12103089, U2031209, U2038105, and U1831207)+8 种基金the National Key Research and Development Program of China (Grant Nos. 2019YFA0405100, 2017YFA0402602, 2018YFA0404204, and 2016YFA0400801)Key Research Program of the Chinese Academy of Sciences (Grant No. QYZDJ-SSW-SLH021)Natural Science Foundation of Jiangsu Province (Grant No. BK20211000)Cultivation Project for FAST Scientific Payoff and Research Achievement of CAMS-CAS, the Strategic Priority Research Program on Space Science, the Western Light Youth Project of Chinese Academy of Sciences (Grant Nos. XDA15360000, XDA15052700, and XDB23040400)funding from the MaxPlanck Partner Group, the science research grants from the China Manned Space Project (Grant Nos. CMS-CSST2021-B11 and CMS-CSST-2021-A11)PKU development (Grant No. 7101502590)support from the XPLORER PRIZEsupported by Fundamental Research Funds for the Central Universities (Grant No. 14380046)the Program for Innovative Talents, Entrepreneur in Jiangsu。
文摘Fast radio bursts(FRBs) are highly dispersed millisecond-duration radio bursts,[1,2]of which the physical origin is still not fully understood. FRB 20201124A is one of the most actively repeating FRBs. In this paper, we present the collection of 1863 burst dynamic spectra of FRB 20201124A measured with the Five-hundred-meter Aperture Spherical radio Telescope(FAST). The current collection, taken from the observation during the FRB active phase from April to June 2021, is the largest burst sample detected for any FRB so far. The standard PSRFITs format is adopted, including dynamic spectra of the burst, and the time information of the dynamic spectra, in addition, mask files help readers to identify the pulse positions are also provided. The dataset is available in Science Data Bank, with the link https://www.doi.org/10.57760/sciencedb.j00113.00076.
基金Supported by Outstanding Graduate Entering Laboratory Project of College of FisheriesGuangdong Ocean University+3 种基金National Natural Science Foundation of China (32073015)Natural Science Foundation of Guangdong Province(2021A1515011078)Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University (CXXL2022005)Undergraduate Innovation Team of Guangdong Ocean University (CCTD201802)
文摘[Objectives]The paper was to clone and analyze bioinformatics of vscN gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the vscN gene sequence of V.alginolyticus HY9901.The full length of vscN gene was amplified by PCR and bioinformatics analysis was performed.[Results]The vscN gene was 1323 bp in total,encoding 440 amino acids,with the theoretical molecular weight of 47.86 kD and the theoretical pI value of 5.89.The online prediction showed that there was no signal peptide and no transmembrane region in VscN.The amino acid sequence had 10 N-myristoylation sites,8 phosphorylation sites(2 protein kinase C phosphorylation sites,6 casein kinase II phosphorylation sites),1 amidation site,11 microbody C-terminal target signal sites,1 ATP/GTP binding site motif A(P ring),and 1 ATPaseαandβsubunit specific site.Homology analysis showed that the VscN protein of V.alginolyticus had high homology with that of V.antiquarius,with a similarity of 95.14%.Phylogenetic tree analysis showed that the VscN of V.alginolyticus was clustered into the same subgroup as that of V.diabolicus and V.antiquarius.Functional domain analysis of VscN protein showed that it had Pfam and AAA domains,and involved in the regulation of bacterial virulence.The three-dimensional structure model of VscN simulated by SWISS-MODEL software was similar to the structure of flagellate-specific ATPase FliH-FliI complex.[Conclusions]The results lay a foundation for further study on the regulatory mechanism of VscN protein on bacterial virulence.
基金Supported by Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean UniversityGrants from Shenzhen Science and Technology Project(JCYJ20190813104207152)+3 种基金National Natural Science Foundation of China(32073015)Natural Science Foundation of Guangdong Province(2021A1515011078)Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2022005)Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).
文摘[Objectives]To clone and analyze the vscB gene of Vibrio alginolyticus HY9901 by bioinformatics.[Methods]A pair of specific primers were designed according to the vscB gene sequence of Vibrio alginolyticus HY9901.The full length of the primers was cloned by PCR and analyzed by bioinformatics.[Results]The vscB gene was 429 bp long,encoding 142 amino acids,with a theoretical molecular weight of 16.4 kDa and a pI value of 5.48.Amino acid sequence analysis of VscB showed that VscB was not a secretory protein,without signal peptide and transmembrane region,and there were protein kinase C phosphorylation site and casein kinase II phosphorylation site in the sequence.Homologous comparison of amino acid sequences showed that VscB of V.alginolyticus had the highest protein similarity with Vibrio Parahaemolyticus,reaching 91%.Phylogenetic tree analysis showed that the corresponding proteins of V.alginolyticus VscB,Vibrio Parahaemolyticus and Vibrio diabolicus were clustered in the same subfamily.Functional domain analysis showed that it had CesT family domain.Tertiary structure prediction showed that there were 3α-helices and 5β-turns in VscB protein.[Conclusions]This study provided a theoretical basis for further study on the function of chaperone of V.alginolyticus.
基金Supported by Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean UniversityNational Natural Science Foundation of China(32073015)+2 种基金Natural Science Foundation of Guangdong Province(2021A1515011078)Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2022005)Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).
文摘[Objectives]To amplify the h-ns gene of Vibrio alginolyticus and analyze it by bioinformatics.[Methods]According to the h-ns gene sequence of V.alginolyticus HY9901,a pair of specific primers were designed and amplified by PCR.[Results]The h-ns gene was 408 bp in length and 135 amino acids were encoded.The predicted theoretical protein molecular weight was about 14.98 kD,and the isoelectric point was 4.99.Protein subcellular localization,SignalP 5.0,TMHMM Server 2.0 and SoftBerry-Psite predictions showed that H-NS was located outside the cell membrane,and the protein was unstable and hydrophobic.There was no signal peptide cleavage site,no transmembrane region and no KEGG metabolic pathway.The amino acid sequence contained three phosphorylation sites,one N-terminal myristoylation site and three microsomal C-terminal target signal sites.Using MEGA 5.0,H-NS phylogenetic tree was constructed by ortho-connection method.The results showed that H-NS of V.alginolyticus was closer to H-NS of Vibrio diabolicus.Using SWISS-MODEL,the three-dimensional structure model of H-NS subunit was simulated,which was similar to the crystal structure of Salmonella typhimurium H-NS1-83.[Conclusions]This study lays a foundation for exploring the regulation mechanism of V.alginolyticus H-NS protein on bacterial virulence in the future.
基金Supported by Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean UniversityNational Natural Science Foundationof China(32073015)+2 种基金Natural Science Foundation of Guangdong Province(2021A1515011078)Undergraduate Innovation and Entrepreneurship Train-ing Program of Guangdong Ocean University(CXXL2022005)UndergraduateInnovation Team of Guangdong Ocean University(CCTD201802)。
文摘[Objectives]The paper was to clone and analyze bioinformatics of ndk gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the ndk gene sequence of V.alginolyticus HY9901.The full length of ndk gene was amplified by PCR and bioinformatics analysis was performed.MEGA 5.0 software was used to construct NDK phylogenetic tree by neighbor-joining method.SWISS-MODEL program was used to obtain the three-dimensional structural model of single subunit from NDK protein.[Results]The ndk gene,molecular structural formula C702H1094N192O214S7,was 426 bp in total,encoding 141 amino acids,with the theoretical molecular weight of 15.87199 kD and the theoretical pI value of 5.13.The prediction results of protein subcellular localization,SignalP 5.0,TMHMM Server 2.0 and SoftBerry-Psite showed that NDK mainly existed in the cytoplasm,and the protein was unstable and hydrophobic.There was neither signal peptide cleavage site,nor transmembrane region and KEGG metabolic pathway.The amino acid sequence had two protein kinase C phosphorylation sites,a casein kinaseⅡphosphorylation site,a N-myristoylation site,three microbody C-terminal target signal sites,and a nucleoside diphosphate kinase active site.Homology analysis showed that the NDK of V.alginolyticus had high homology with that of V.diabolicus,with a similarity of 98.58%.Analysis of the structural functional domain revealed that the protein had one NDK structural functional domain.The prediction results of secondary structure showed that theα-helix,random coil,β-sheet and extended strand accounted for 53.19%,28.37%,7.09%and 11.35%,respectively.Analysis of NDK protein via STRING database demonstrated that the proteins interacting with NDK protein were NrdA,NrdB,GmK,CmK,TmK,PyrG,PyrH,RelA,FolE and SpoT.[Conclusions]The study plays a positive role in the prevention and control of vibriosis and the improvement of the current aquaculture environment.
基金supported by The Graduate University for Advanced Studies (SOKENDAI)supported by the Japanese Government (MEXT) Scholarship+18 种基金supported by JSPS KAKENHI Grant Numbers JP18K03656 (M.K.),JP18H03721 (K.N.,K.H.and M.K.),JP19H01943 (K.H.,F.T.and Y.H.) and JP18KK0090 (K.H.and F.T.)supported by the Mitsubishi Foundation (grant number 201911019)supported by an EACOA Fellowship awarded by the East Asia Core Observatories Association,which consists of the Academia Sinica Institute of Astronomy and Astrophysics,the National Astronomical Observatory of Japan,the Center for Astronomical Mega-Science,the Chinese Academy of Sciencesthe Korea Astronomy and Space Science Institutethe financial support from the National Research Foundation (NRF) of Korea via Global Ph.D.Fellowship Grant 2014H1A2A1018695 and 2015H1A2A1033752,respectivelysupport from the NRF via Grant 2019R1F1A1059721supported by the Major Program of the National Natural Science Foundation of China (NSFC,Grant Nos.11590780 and 11590784)the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No.KJCX1-YW-18)the Scientific Program of Shanghai Municipality (08DZ1160100)Key Laboratory for Radio Astronomy,CASsupport from NSFC (Grant No.11803071)supported by the National Key R&D Program of China (Grant No.2018YFA0404602)the CAS ‘Light of West China’ Program (Grant No.2018-XBQNXZ-B021)the NSFC (Grant Nos.U2031212 and 61931002)the Youth Innovation Promotion Association of the CAS (Grant No.2017084)support from Fundamental Research Grant Scheme (FRGS) FRGS/1/2019/STG02/UM/02/6supported by the Max Planck Partner Group of the MPG and the CASthe support by the Key Program of the NSFC (Grant No.11933007)the Research Program of Fundamental and Frontier Sciences,CAS (Grant No.ZDBS-LY-SLH011)。
文摘The East Asian Very Long Baseline Interferometry(VLBI) Network(EAVN) is a rapidly evolving international VLBI array that is currently promoted under joint efforts among China,Japan and Korea.EAVN aims at forming a joint VLBI Network by combining a large number of radio telescopes distributed over East Asian regions.After the combination of the Korean VLBI Network(KVN) and the VLBI Exploration of Radio Astrometry(VERA) into Ka VA,further expansion with the joint array in East Asia is actively promoted.Here we report the first imaging results(at 22 and 43 GHz) of bright radio sources obtained with Ka VA connected to Tianma 65-m and Nanshan 26-m Radio Telescopes in China.To test the EAVN imaging performance for different sources,we observed four active galactic nuclei(AGN) having different brightness and morphology.As a result,we confirmed that the Tianma 65-m Radio Telescope(TMRT) significantly enhances the overall array sensitivity,a factor of 4 improvement in baseline sensitivity and 2 in image dynamic range compared to the case of Ka VA only.The addition of the Nanshan 26-m Radio Telescope(NSRT) further doubled the east-west angular resolution.With the resulting high-dynamic-range,high-resolution images with EAVN(Ka VA+TMRT+NSRT),various fine-scale structures in our targets,such as the counter-jet in M87,a kink-like morphology of the 3 C 273 jet and the weak emission in other sources are successfully detected.This demonstrates the powerful capability of EAVN to study AGN jets and to achieve other science goals in general.Ongoing expansion of EAVN will further enhance the angular resolution,detection sensitivity and frequency coverage of the network.
