DEE （diesel exhaust emitted） and fossil fuel do contribute to the pollution of ecosystem while very little is known about their toxic effects on pulmonary tissue in the short-term. Ten male Guinea pigs exposed to DE...DEE （diesel exhaust emitted） and fossil fuel do contribute to the pollution of ecosystem while very little is known about their toxic effects on pulmonary tissue in the short-term. Ten male Guinea pigs exposed to DEE in an open system experiment at different time schemes and their tracheas and lungs were processed for routine Histology, Immunohistochemsitry and TEM （transmission Electron Microscopy） techniques. The results revealed early toxicological effects of DEE on pulmonary tissue involved cellular damages to the mucosa of trachea and bronchial tree, rupture of alveolar walls, disintegration of pneumocytes, development of mucous secretions, swellings in the alveolar network, hemorrhage and lymphatic infiltration all over the parenchyma starting from the early hours of exposure to DEE. Ultrastructural changes confirmed the ruptures to the alveolar walls, infiltration of various WBC （white blood cells）, development of vacuoles inside the cytoplasmic organelles of pnemocytes, distortion in morphology of both RBCs （Red Blood corpuscles） which all had begun as early as 1 h following the exposure to the DEE. Activation of the immune system was noted via the increase in size of lymph nodules to confront the invaded particulates. These results indicate blockage of the respiratory leading to suffocation and do alert the expected acute cellular harms to the pulmonary tissues following exposure to the DEE of mechanics work in vicinity of DEE who spend most daily hours in semi-closed areas.展开更多
文摘DEE （diesel exhaust emitted） and fossil fuel do contribute to the pollution of ecosystem while very little is known about their toxic effects on pulmonary tissue in the short-term. Ten male Guinea pigs exposed to DEE in an open system experiment at different time schemes and their tracheas and lungs were processed for routine Histology, Immunohistochemsitry and TEM （transmission Electron Microscopy） techniques. The results revealed early toxicological effects of DEE on pulmonary tissue involved cellular damages to the mucosa of trachea and bronchial tree, rupture of alveolar walls, disintegration of pneumocytes, development of mucous secretions, swellings in the alveolar network, hemorrhage and lymphatic infiltration all over the parenchyma starting from the early hours of exposure to DEE. Ultrastructural changes confirmed the ruptures to the alveolar walls, infiltration of various WBC （white blood cells）, development of vacuoles inside the cytoplasmic organelles of pnemocytes, distortion in morphology of both RBCs （Red Blood corpuscles） which all had begun as early as 1 h following the exposure to the DEE. Activation of the immune system was noted via the increase in size of lymph nodules to confront the invaded particulates. These results indicate blockage of the respiratory leading to suffocation and do alert the expected acute cellular harms to the pulmonary tissues following exposure to the DEE of mechanics work in vicinity of DEE who spend most daily hours in semi-closed areas.
