A total of 10 specimens of Alcyonacea corals were collected at depths ranging from 905 m to 1633 m by the manned submersible Shenhai Yongshi during two cruises in the South China Sea(SCS).Based on mitochondrial genomi...A total of 10 specimens of Alcyonacea corals were collected at depths ranging from 905 m to 1633 m by the manned submersible Shenhai Yongshi during two cruises in the South China Sea(SCS).Based on mitochondrial genomic characteristics,morphological examination,and sclerite scanning electron microscopy,the samples were categorized into four suborders(Calcaxonia,Holaxonia,Scleraxonia,and Stolonifera),and identified as 9 possible new cold-water coral species.Assessments of GC-skew dissimilarity,phylogenetic distance,and average nucleotide identity(ANI)revealed a slow evolutionary rate for the octocoral mitochondrial sequences.The nonsynonymous(Ka)to synonymous(Ks)substitution ratio(Ka/Ks)suggested that the 14 protein-coding genes(PCGs)were under purifying selection,likely due to specific deep-sea environmental pressures.Correlation analysis of the median Ka/Ks values of five gene families and environmental factors indicated that the genes encoding cytochrome b(cyt b)and DNA mismatch repair protein(mutS)may be influenced by environmental factors in the context of deep-sea species formation.This study highlights the slow evolutionary pace and adaptive mechanisms of deep-sea corals.展开更多
BACKGROUND Compare the diagnostic performance of ultrasound(US),magnetic resonance imaging(MRI),and serum tumor markers alone or in combination for detecting ovarian tumors.AIM To investigate the diagnostic value of U...BACKGROUND Compare the diagnostic performance of ultrasound(US),magnetic resonance imaging(MRI),and serum tumor markers alone or in combination for detecting ovarian tumors.AIM To investigate the diagnostic value of US,MRI combined with tumor markers in ovarian tumors.METHODS The data of 110 patients with ovarian tumors,confirmed by surgery and pathology,were collected in our hospital from February 2018 to May 2023.The dataset included 60 cases of benign tumors and 50 cases of malignant tumors.Prior to surgery,all patients underwent preoperative US and MRI examinations,as well as serum tumor marker tests[carbohydrate antigen 125(CA125),human epididymis protein 4(HE4)].The aim of the study was to compare the diagnostic performance of these three methods individually and in combination for ovarian tumors.RESULTS This study found statistically significant differences in the ultrasonic imaging characteristics between benign and malignant tumors.These differences include echo characteristics,presence or absence of a capsule,blood flow resistance index,clear tumor shape,and blood flow signal display rate(P<0.05).The apparent diffusion coefficient values of the solid and cystic parts in benign tumors were found to be higher compared to malignant tumors(P<0.05).Additionally,the time-intensity curve image features of benign and malignant tumors showed significant statistical differences(P<0.05).The levels of serum CA125 and HE4 in benign tumors were lower than those in malignant tumors(P<0.05).The combined use of US,MRI,and tumor markers in the diagnosis of ovarian tumors demonstrates higher accuracy,sensitivity,and specificity compared to using each method individually(P<0.05).CONCLUSION US,MRI,and tumor markers each have their own advantages and disadvantages when it comes to diagnosing ovarian tumors.However,by combining these three methods,we can significantly enhance the accuracy of ovarian tumor diagnosis,enabling early detection and identification of the tumor’s nature,and providing valuable guidance for clinical treatment.展开更多
BACKGROUND It remains controversial as to which pathological classification is most valuable in predicting the overall survival(OS)of patients with gastric cancer(GC).AIM To assess the prognostic performances of three...BACKGROUND It remains controversial as to which pathological classification is most valuable in predicting the overall survival(OS)of patients with gastric cancer(GC).AIM To assess the prognostic performances of three pathological classifications in GC and develop a novel prognostic nomogram for individually predicting OS.METHODS Patients were identified from the Surveillance,Epidemiology,and End Results program.Univariate and multivariate analyses were performed to identify the independent prognostic factors.Model discrimination and model fitting were evaluated by receiver operating characteristic curves and Akaike information criteria.Decision curve analysis was performed to assess clinical usefulness.The independent prognostic factors identified by multivariate analysis were further applied to develop a novel prognostic nomogram.RESULTS A total of 2718 eligible GC patients were identified.The modified Lauren classification was identified as one of the independent prognostic factors for OS.It showed superior model discriminative ability and model-fitting performance over the other pathological classifications,and similar results were obtained in various patient settings.In addition,it showed superior net benefits over the Lauren classification and tumor differentiation grade in predicting 3-and 5-year OS.A novel prognostic nomogram incorporating the modified Lauren classification showed superior model discriminative ability,model-fitting performance,and net benefits over the American Joint Committee on Cancer 8th edition tumor-nodemetastasis classification.CONCLUSION The modified Lauren classification shows superior net benefits over the Lauren classification and tumor differentiation grade in predicting OS.A novel prognostic nomogram incorporating the modified Lauren classification shows good model discriminative ability,model-fitting performance,and net benefits.展开更多
This study is to report a ZnSe quantum dot with a large two-photon absorption cross section and good biocompatibility,which can be used in bioimaging.Fluorescence emission at 410 nm is observed in the quantum dot unde...This study is to report a ZnSe quantum dot with a large two-photon absorption cross section and good biocompatibility,which can be used in bioimaging.Fluorescence emission at 410 nm is observed in the quantum dot under 760-nm laser excitation.These biocompatible quantum dots exhibit a two-photon cross-section of 9.1×105 GM(1 GM=10-50 cm4·s/photon).Two-photon excited laser scanning microscopic images show that cells co-cultured with ZnSe quantum dots are found in the blue channel at a fluorescence intensity that is 14.5 times that of control cells not cocultured with quantum dots.After incubating zebrafish larvae with ZnSe quantum dots for 24 h,the fluorescence intensity of the yolk sac stimulated by ultraviolet light is 2.9 times that of the control group.The proposed material shows a great potential application in biological imaging.展开更多
Objective: To study the effects of resveratrol on apoptosis of rat brain during ischemia-reperfusion and on NLRP3 inflammatory bodies, Caspase-1 and ZO-1 in microglia. Methods:According to the random digital table met...Objective: To study the effects of resveratrol on apoptosis of rat brain during ischemia-reperfusion and on NLRP3 inflammatory bodies, Caspase-1 and ZO-1 in microglia. Methods:According to the random digital table method, forty-five male Sprague-Dawley rats were divided into the sham-operated group (blank control group), cerebral ischemia-reperfusion group (IR control group) and resveratrol treatment group, with 15 rats in each group. After the establishment of cerebral ischemia-reperfusion model by suture method, the effects of different methods on the expression of apoptosis-related molecules (promoters, suppressors) in brain tissue, microglial NLRP3 inflammatory bodies, Caspase-1 and ZO-1 in SD rats were compared. Results: The content of apoptosis-related molecules (CytC, AIF, Caspase-8, Caspase-9, Fas, FasL) in the sham-operated group was significantly lower than that in IR control group and resveratrol group, and the content in IR control group was significantly higher than that in resveratrol group (P<0.05). The content of anti-apoptosis-related molecules (Livin, Survivin, XIAP) in sham-operated group was significantly higher than that in IR control group and resveratrol group, while the content of pro-apoptosis-related molecules in IR control group was significantly lower than that in resveratrol group (P<0. 05). The levels of IL-10, IL-6,TNF-α of brain tissue in resveratrol group were significantly higher than those in the control group and significantly lower than those in IR group (P<0. 05). The content of NLRP3 inflammatory body in microglia was the lowest in the sham-operated group, followed by resveratrol group, IR control group. ZO-1 content was the highest in the sham-operated group, followed by resveratrol group and IR control group. Conclusion: Resveratrol can effectively inhibit cellular pyrolysis, increase the level of NLRP3 inflammatory body and Caspase-1 and decrease the level of ZO-1 in rat brain tissue during ischemia-reperfusion, so as to play a protective role in the brain.展开更多
While heterogeneous single-atom catalysts(SACs)have achieved great success in the past decade,their application is potentially limited by their simplistic single-atom active centers,which make single-cluster catalysts...While heterogeneous single-atom catalysts(SACs)have achieved great success in the past decade,their application is potentially limited by their simplistic single-atom active centers,which make single-cluster catalysts(SCCs)a natural extension in the domain of heterogeneous catalysis.SCCs with precise numbers of atoms and structural configurations possess SAC merits,yet have greater potential for catalyzing complex reactions and/or bulky reactants.Through systematic quantum-chemical studies and computational screening,we report here the rational design of transition metal three-atom clusters anchored on graphdiyne(GDY)as a novel kind of stable SCC with great promise for efficient and atomically precise heterogenous catalysis.By investigating their structure and catalytic performance for the oxygen reduction reaction,the hydrogen evolution reaction,and the CO_(2)reduction reaction,we have provided theoretical guidelines for their potential applications as heterogeneous catalysts.These GDY-supported three-atom SCCs provide an ideal benchmark scaffold for rational design of atomically precise heterogeneous catalysts for industrially important chemical reactions.展开更多
The aim of this study is to assess the antibacterial and anti-biofilm properties of the lipid extract from Man- tidis ootheca against the gentamycin resistant Pseudomonas aeruginosa. The chemical composition of the li...The aim of this study is to assess the antibacterial and anti-biofilm properties of the lipid extract from Man- tidis ootheca against the gentamycin resistant Pseudomonas aeruginosa. The chemical composition of the lipid extract and its relative proportion were determined using the technique of gas chromatography coupled with mass spec- trometry （GC-MS）. Antibacterial susceptibility tests were performed using a disc diffusion assay and the minimum inhibition concentration （MIC） was determined by way of the agar dilution method. The anti-biofilm test was carried out with crystal violet staining and scanning electron microscopy （SEM）. There were 16 compounds detected, and the most abundant components were sesquiterpenoids, monoterpenes, and trace aromatic compounds. The MIC for P. aeruginosa was 4 mg/ml and the eradication effect on preformed biofilms was established and compared with a ciprofloxacin control. The results of our study indicated that a lipid extract from M. ootheca could be used as a topical and antibacterial agent with anti-biofilm activity in the future.展开更多
Background:In addition to the stepwise manner of lymph-node metastasis from the primary tumour,the skip lymph-node metastasis(SLNM)was identified as a low-incidence metastasis of gastric cancer(GC).So far,both the mec...Background:In addition to the stepwise manner of lymph-node metastasis from the primary tumour,the skip lymph-node metastasis(SLNM)was identified as a low-incidence metastasis of gastric cancer(GC).So far,both the mechanism and outcome of SLNM have not been elucidated completely.The purpose of this study was to analyse the clinical significance and the potential mechanism of SLNM in GC patients who had lymph-node metastasis.Methods:Clinicopathological data and follow-up information of 505 GC patients who had lymph-node metastasis were analysed to demonstrate the significance of SLNM in evaluating the prognostic outcome.According to the pathological results,all GC patients who had lymph-node metastasis were categorized into three groups:patients with the perigastric lymphnode metastasis,patients with the perigastric and extragastric lymph-node metastasis and patients with SLNM.Results:Among the 505 GC patients who had lymph-node metastasis,24(4.8%)had pathologically identified SLNM.The location of lymph-node metastasis was not significantly associated with 5-year survival rate and overall survival(OS)(P=0.194).The stratified survival analysis results showed that the status of SLNM was significantly associated with the OS in patients with pN1 GC(P=0.001).The median OS was significantly shorter in 19 pN1 GC patients with SLNM than in 100 patients with perigastric lymph-node metastasis(P<0.001).The case–control matched logistic regression analysis results showed that tumour size(P=0.002)was the only clinicopathological factor that may predict SLNM in pN1 GC patients undergoing curative surgery.Among the 19 pN1 GC patients with SLNM,17(89.5%)had metastatic lymph nodes along the common hepatic artery,around the celiac artery or in the hepatoduodenal ligament.Conclusions:SLNM may be considered a potentially practicable indicator for prognosis among various subgroups of pN1 GC patients.展开更多
The low valence electron concentration(VEC)Al_(x)CoCrFeNiSi(x=0.5,1.0,1.5 and 2.0)high-entropy alloys(HEAs)were designed by the fundamental properties of the constituent elements and prepared by vacuum arc melting met...The low valence electron concentration(VEC)Al_(x)CoCrFeNiSi(x=0.5,1.0,1.5 and 2.0)high-entropy alloys(HEAs)were designed by the fundamental properties of the constituent elements and prepared by vacuum arc melting method.The effects of Al addition on the crystal structure and microstructure were investigated.The microhardness and wear property were also researched.The results showed that the microstructure transformed from dendritic crystal to equiaxed crystal.It was found that FCC phase gradually decreased with the increasing Al content and disappeared until in a composition of 1.0 in Al_(x)CoCrFeNiSi HEAs.Little FCC phase was found with continuously adding Al,while the phase fraction of BCC increased from 85.0% to 91.8%,and VEC decreased from 7.00 to 6.14.The microhardness was increased gradually from 598 up to 909 HV with addition of Al from 0.5 to 2.0.It was the same of the compressive strength results,which improved from 1200 to 1920 MPa.The wear coefficient and mass loss were in line with mechanical properties evolution,which was attributed to the microstructure transformation into equiaxed crystal and the increase in BCC phase.展开更多
FeCoCrNiA1Bx（x=0, 0.25, 0.50, 0.75） coatings were prepared by laser cladding to study the effects of boron on the structure and properties of high-entropy alloys coatings. The microstructure, microhardness, and wear...FeCoCrNiA1Bx（x=0, 0.25, 0.50, 0.75） coatings were prepared by laser cladding to study the effects of boron on the structure and properties of high-entropy alloys coatings. The microstructure, microhardness, and wear resistance properties of the samples were investigated by scanning electron microscopy, X-ray diffraction, metallographic micro-hardness test, and friction wear test, respectively, and the mechanism of the wear behavior was also analyzed. The results showed that the high-entropy alloys consisted of BCC phase and eutectic structure, which contained FCC phase and M2B. With boron addition, the content of BCC phase increased while that of eutectic structure decreased. The wear resistance of the high-entropy coatings was considerably improved with increasing addition of horon, and accordingly, the FeCoCrNiAlB0.75 coating showed the best wear resistance.展开更多
Despite recent advances in antibiotic use, in creasing numbers of human immunodeficiency virus (HIV)- negative patients with Pneumocystis jirovecii pneumonia (PCP) are being admitted to intensive care units (ICUs), an...Despite recent advances in antibiotic use, in creasing numbers of human immunodeficiency virus (HIV)- negative patients with Pneumocystis jirovecii pneumonia (PCP) are being admitted to intensive care units (ICUs), and show a mortality rate of over 50%.[1] A high neutrophil count in bronchoalveolar lavage (BAL) fluid is a predictor of poor prognosis in HIV-negative patients with PCP.展开更多
Yttria-stabilized zirconia（ YSZ） coatings were deposited by low pressure plasma spray（ LPPS） in 1.0× 10^4 Pa,1.5 × 10^4 Pa,and 2.5 × 10^4 Pa. Both in-flight particle diagnostic detected by DPV-2000 ...Yttria-stabilized zirconia（ YSZ） coatings were deposited by low pressure plasma spray（ LPPS） in 1.0× 10^4 Pa,1.5 × 10^4 Pa,and 2.5 × 10^4 Pa. Both in-flight particle diagnostic detected by DPV-2000 system and ANSYS-FLUENT software were used to study the connection between the parameters of flying particles and the coating formation,which might help to recognize the relationship between the operation parameters and the coatings quality. The results of simulation showed that particles in a lower spray pressure could achieve a higher velocity. The particle velocity was around 380 m/s at a distance of 35 cm from the nozzle at 1.0 × 10^4 Pa while only 300 m/s at 2.5 × 10^4 Pa in actual measurement.The results showed that the velocity of particles increased with decreasing the spray pressure,which might enhance the flattening rate of coatings and thereby decreased the porosity. The deposited YSZ coating with the lowest porosity can be gained under 1.0 × 10^4 Pa condition.展开更多
FeCoCrNiAl high entropy alloy coatings were prepared by supersonic air-plasma spraying.The coatings were post-treated by vacuum heat treatment at 600 and 900°C,and laser re-melting with 300 W,respectively,to stud...FeCoCrNiAl high entropy alloy coatings were prepared by supersonic air-plasma spraying.The coatings were post-treated by vacuum heat treatment at 600 and 900°C,and laser re-melting with 300 W,respectively,to study the influence of different treatments on the structure and properties of the coatings.The phase constitution,microstructure and microhardness of the coatings after treatments were investigated using X-ray diffraction,scanning electron microscopy and energy dispersive spectrometry.Results showed that the as-sprayed coatings consisted of pure metal and Fe-Cr.The AlNi;phase was obtained after the vacuum heat treatment process.A body-centered cubic structure with less AlNi;could be found in the coating after the laser re-melting process.The average hardness values of the as-sprayed coating and the coatings with two different temperature vacuum heat treatments and with laser re-melting were 177,227,266 and 682 HV,respectively.This suggests that the vacuum heat treatment promoted the alloying process of the coatings,and contributed to the enhancement of the coating wear resistance.The laser re-melted coating showed the best wear resistance.展开更多
To the Editor:Following H7N9infection,the host lymphocyte immunity plays an antiviral role.[l] Furthermore,low frequencies of T-cells correlate with disease severity.[2]Herein,we present a H7N9-infected patient with l...To the Editor:Following H7N9infection,the host lymphocyte immunity plays an antiviral role.[l] Furthermore,low frequencies of T-cells correlate with disease severity.[2]Herein,we present a H7N9-infected patient with life-threatening lymphopenia (only 0.06× 10^9/L in the peripheral blood),which we have barely ever seen before.We also measured the proportidns of T-cell subpopulations in the blood and bronchoalveolar lavage fluid (BALF).展开更多
To verify the microalloying function and segregation behavior of trace Mg at grain boundary in steel,the 2.25Cr–1Mo steel doped with 0.056%P containing different Mg contents was refined with a vacuum-induction furnac...To verify the microalloying function and segregation behavior of trace Mg at grain boundary in steel,the 2.25Cr–1Mo steel doped with 0.056%P containing different Mg contents was refined with a vacuum-induction furnace.The effects of trace Mg addition on the temper embrittlement susceptibility of 2.25Cr–1Mo steel were studied by step-cooling test and the segregation behavior of Mg at grain boundary was explored by Auger electron spectroscopy.It is shown that P-induced temper embrittlement susceptibility can be reduced after subjecting to step-cooling treatment with trace Mg addition,mainly benefited from the segregation of Mg at grain boundary.This segregation can decrease the segregation amounts of P and S,especially for P,and increase the grain boundary cohesion,reducing the adverse effect on temper embrittlement caused by P and S.展开更多
基金supported by the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2022QNLM030004)Hainan Science and Technology Department(ZDKJ2019011)+2 种基金Open Project Fund of Key Laboratory of Sustainable Development of Polar Fisheries,Ministry of Agriculture and Rural Affairs of PRC(2022OPF02)State Key R&D Project(2021YFF0502500)Qingdao Postdoctoral Applied Research Project(JZ2223j06100)。
文摘A total of 10 specimens of Alcyonacea corals were collected at depths ranging from 905 m to 1633 m by the manned submersible Shenhai Yongshi during two cruises in the South China Sea(SCS).Based on mitochondrial genomic characteristics,morphological examination,and sclerite scanning electron microscopy,the samples were categorized into four suborders(Calcaxonia,Holaxonia,Scleraxonia,and Stolonifera),and identified as 9 possible new cold-water coral species.Assessments of GC-skew dissimilarity,phylogenetic distance,and average nucleotide identity(ANI)revealed a slow evolutionary rate for the octocoral mitochondrial sequences.The nonsynonymous(Ka)to synonymous(Ks)substitution ratio(Ka/Ks)suggested that the 14 protein-coding genes(PCGs)were under purifying selection,likely due to specific deep-sea environmental pressures.Correlation analysis of the median Ka/Ks values of five gene families and environmental factors indicated that the genes encoding cytochrome b(cyt b)and DNA mismatch repair protein(mutS)may be influenced by environmental factors in the context of deep-sea species formation.This study highlights the slow evolutionary pace and adaptive mechanisms of deep-sea corals.
文摘BACKGROUND Compare the diagnostic performance of ultrasound(US),magnetic resonance imaging(MRI),and serum tumor markers alone or in combination for detecting ovarian tumors.AIM To investigate the diagnostic value of US,MRI combined with tumor markers in ovarian tumors.METHODS The data of 110 patients with ovarian tumors,confirmed by surgery and pathology,were collected in our hospital from February 2018 to May 2023.The dataset included 60 cases of benign tumors and 50 cases of malignant tumors.Prior to surgery,all patients underwent preoperative US and MRI examinations,as well as serum tumor marker tests[carbohydrate antigen 125(CA125),human epididymis protein 4(HE4)].The aim of the study was to compare the diagnostic performance of these three methods individually and in combination for ovarian tumors.RESULTS This study found statistically significant differences in the ultrasonic imaging characteristics between benign and malignant tumors.These differences include echo characteristics,presence or absence of a capsule,blood flow resistance index,clear tumor shape,and blood flow signal display rate(P<0.05).The apparent diffusion coefficient values of the solid and cystic parts in benign tumors were found to be higher compared to malignant tumors(P<0.05).Additionally,the time-intensity curve image features of benign and malignant tumors showed significant statistical differences(P<0.05).The levels of serum CA125 and HE4 in benign tumors were lower than those in malignant tumors(P<0.05).The combined use of US,MRI,and tumor markers in the diagnosis of ovarian tumors demonstrates higher accuracy,sensitivity,and specificity compared to using each method individually(P<0.05).CONCLUSION US,MRI,and tumor markers each have their own advantages and disadvantages when it comes to diagnosing ovarian tumors.However,by combining these three methods,we can significantly enhance the accuracy of ovarian tumor diagnosis,enabling early detection and identification of the tumor’s nature,and providing valuable guidance for clinical treatment.
基金Supported by The China Scholarship Council,No.201908050148.
文摘BACKGROUND It remains controversial as to which pathological classification is most valuable in predicting the overall survival(OS)of patients with gastric cancer(GC).AIM To assess the prognostic performances of three pathological classifications in GC and develop a novel prognostic nomogram for individually predicting OS.METHODS Patients were identified from the Surveillance,Epidemiology,and End Results program.Univariate and multivariate analyses were performed to identify the independent prognostic factors.Model discrimination and model fitting were evaluated by receiver operating characteristic curves and Akaike information criteria.Decision curve analysis was performed to assess clinical usefulness.The independent prognostic factors identified by multivariate analysis were further applied to develop a novel prognostic nomogram.RESULTS A total of 2718 eligible GC patients were identified.The modified Lauren classification was identified as one of the independent prognostic factors for OS.It showed superior model discriminative ability and model-fitting performance over the other pathological classifications,and similar results were obtained in various patient settings.In addition,it showed superior net benefits over the Lauren classification and tumor differentiation grade in predicting 3-and 5-year OS.A novel prognostic nomogram incorporating the modified Lauren classification showed superior model discriminative ability,model-fitting performance,and net benefits over the American Joint Committee on Cancer 8th edition tumor-nodemetastasis classification.CONCLUSION The modified Lauren classification shows superior net benefits over the Lauren classification and tumor differentiation grade in predicting OS.A novel prognostic nomogram incorporating the modified Lauren classification shows good model discriminative ability,model-fitting performance,and net benefits.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61774062 and U20A20206)the Science and Techology Program of Guangzhou City,China(Grant No.2019050001)the Natural Science Foundation of Guangdong Province,China(Grant Nos.2018A030313854 and2016A030308010)。
文摘This study is to report a ZnSe quantum dot with a large two-photon absorption cross section and good biocompatibility,which can be used in bioimaging.Fluorescence emission at 410 nm is observed in the quantum dot under 760-nm laser excitation.These biocompatible quantum dots exhibit a two-photon cross-section of 9.1×105 GM(1 GM=10-50 cm4·s/photon).Two-photon excited laser scanning microscopic images show that cells co-cultured with ZnSe quantum dots are found in the blue channel at a fluorescence intensity that is 14.5 times that of control cells not cocultured with quantum dots.After incubating zebrafish larvae with ZnSe quantum dots for 24 h,the fluorescence intensity of the yolk sac stimulated by ultraviolet light is 2.9 times that of the control group.The proposed material shows a great potential application in biological imaging.
基金supported by the Medical Science Research Project of Hebei Province in 2019(Grant No.20191327).
文摘Objective: To study the effects of resveratrol on apoptosis of rat brain during ischemia-reperfusion and on NLRP3 inflammatory bodies, Caspase-1 and ZO-1 in microglia. Methods:According to the random digital table method, forty-five male Sprague-Dawley rats were divided into the sham-operated group (blank control group), cerebral ischemia-reperfusion group (IR control group) and resveratrol treatment group, with 15 rats in each group. After the establishment of cerebral ischemia-reperfusion model by suture method, the effects of different methods on the expression of apoptosis-related molecules (promoters, suppressors) in brain tissue, microglial NLRP3 inflammatory bodies, Caspase-1 and ZO-1 in SD rats were compared. Results: The content of apoptosis-related molecules (CytC, AIF, Caspase-8, Caspase-9, Fas, FasL) in the sham-operated group was significantly lower than that in IR control group and resveratrol group, and the content in IR control group was significantly higher than that in resveratrol group (P<0.05). The content of anti-apoptosis-related molecules (Livin, Survivin, XIAP) in sham-operated group was significantly higher than that in IR control group and resveratrol group, while the content of pro-apoptosis-related molecules in IR control group was significantly lower than that in resveratrol group (P<0. 05). The levels of IL-10, IL-6,TNF-α of brain tissue in resveratrol group were significantly higher than those in the control group and significantly lower than those in IR group (P<0. 05). The content of NLRP3 inflammatory body in microglia was the lowest in the sham-operated group, followed by resveratrol group, IR control group. ZO-1 content was the highest in the sham-operated group, followed by resveratrol group and IR control group. Conclusion: Resveratrol can effectively inhibit cellular pyrolysis, increase the level of NLRP3 inflammatory body and Caspase-1 and decrease the level of ZO-1 in rat brain tissue during ischemia-reperfusion, so as to play a protective role in the brain.
基金This work was financially supported by the National Natural Science Foundation of China(grant no.22033005 to J.L.and grant no.21903047 to H.X.)The support of Guangdong Provincial Key Laboratory of Catalysis(grant no.2020B121201002)is also acknowledged.The calculations were performed using the supercomputers at Tsinghua National Laboratory for Information Science and Technology,the Computational Chemistry Laboratory of the Department of Chemistry under the Tsinghua Xuetang Talents Program,and the Supercomputer Center of the Southern University of Science and Technology.
文摘While heterogeneous single-atom catalysts(SACs)have achieved great success in the past decade,their application is potentially limited by their simplistic single-atom active centers,which make single-cluster catalysts(SCCs)a natural extension in the domain of heterogeneous catalysis.SCCs with precise numbers of atoms and structural configurations possess SAC merits,yet have greater potential for catalyzing complex reactions and/or bulky reactants.Through systematic quantum-chemical studies and computational screening,we report here the rational design of transition metal three-atom clusters anchored on graphdiyne(GDY)as a novel kind of stable SCC with great promise for efficient and atomically precise heterogenous catalysis.By investigating their structure and catalytic performance for the oxygen reduction reaction,the hydrogen evolution reaction,and the CO_(2)reduction reaction,we have provided theoretical guidelines for their potential applications as heterogeneous catalysts.These GDY-supported three-atom SCCs provide an ideal benchmark scaffold for rational design of atomically precise heterogeneous catalysts for industrially important chemical reactions.
文摘The aim of this study is to assess the antibacterial and anti-biofilm properties of the lipid extract from Man- tidis ootheca against the gentamycin resistant Pseudomonas aeruginosa. The chemical composition of the lipid extract and its relative proportion were determined using the technique of gas chromatography coupled with mass spec- trometry （GC-MS）. Antibacterial susceptibility tests were performed using a disc diffusion assay and the minimum inhibition concentration （MIC） was determined by way of the agar dilution method. The anti-biofilm test was carried out with crystal violet staining and scanning electron microscopy （SEM）. There were 16 compounds detected, and the most abundant components were sesquiterpenoids, monoterpenes, and trace aromatic compounds. The MIC for P. aeruginosa was 4 mg/ml and the eradication effect on preformed biofilms was established and compared with a ciprofloxacin control. The results of our study indicated that a lipid extract from M. ootheca could be used as a topical and antibacterial agent with anti-biofilm activity in the future.
基金supported in part by grants from the Programs of National Natural Science Foundation of China(No.81572372)the National Key Research and Development Program‘Major Chronic Non-Infectious Disease Research’of China(No.2016YFC1303202)the National Key Research and Development Program‘Precision Medicine Research’of China(No.2017YFC0908304).
文摘Background:In addition to the stepwise manner of lymph-node metastasis from the primary tumour,the skip lymph-node metastasis(SLNM)was identified as a low-incidence metastasis of gastric cancer(GC).So far,both the mechanism and outcome of SLNM have not been elucidated completely.The purpose of this study was to analyse the clinical significance and the potential mechanism of SLNM in GC patients who had lymph-node metastasis.Methods:Clinicopathological data and follow-up information of 505 GC patients who had lymph-node metastasis were analysed to demonstrate the significance of SLNM in evaluating the prognostic outcome.According to the pathological results,all GC patients who had lymph-node metastasis were categorized into three groups:patients with the perigastric lymphnode metastasis,patients with the perigastric and extragastric lymph-node metastasis and patients with SLNM.Results:Among the 505 GC patients who had lymph-node metastasis,24(4.8%)had pathologically identified SLNM.The location of lymph-node metastasis was not significantly associated with 5-year survival rate and overall survival(OS)(P=0.194).The stratified survival analysis results showed that the status of SLNM was significantly associated with the OS in patients with pN1 GC(P=0.001).The median OS was significantly shorter in 19 pN1 GC patients with SLNM than in 100 patients with perigastric lymph-node metastasis(P<0.001).The case–control matched logistic regression analysis results showed that tumour size(P=0.002)was the only clinicopathological factor that may predict SLNM in pN1 GC patients undergoing curative surgery.Among the 19 pN1 GC patients with SLNM,17(89.5%)had metastatic lymph nodes along the common hepatic artery,around the celiac artery or in the hepatoduodenal ligament.Conclusions:SLNM may be considered a potentially practicable indicator for prognosis among various subgroups of pN1 GC patients.
基金financially supported by the Natural Science Foundation of Liaoning Province(No.2019-MS-247)the Liaoning Revitalization Talents Program(XLYC1807178).
文摘The low valence electron concentration(VEC)Al_(x)CoCrFeNiSi(x=0.5,1.0,1.5 and 2.0)high-entropy alloys(HEAs)were designed by the fundamental properties of the constituent elements and prepared by vacuum arc melting method.The effects of Al addition on the crystal structure and microstructure were investigated.The microhardness and wear property were also researched.The results showed that the microstructure transformed from dendritic crystal to equiaxed crystal.It was found that FCC phase gradually decreased with the increasing Al content and disappeared until in a composition of 1.0 in Al_(x)CoCrFeNiSi HEAs.Little FCC phase was found with continuously adding Al,while the phase fraction of BCC increased from 85.0% to 91.8%,and VEC decreased from 7.00 to 6.14.The microhardness was increased gradually from 598 up to 909 HV with addition of Al from 0.5 to 2.0.It was the same of the compressive strength results,which improved from 1200 to 1920 MPa.The wear coefficient and mass loss were in line with mechanical properties evolution,which was attributed to the microstructure transformation into equiaxed crystal and the increase in BCC phase.
基金funded by National Natural Science Foundation of China(No.51301112)Natural Science Foundation of Liaoning Province of China (No.201602553)Science Research Program of Education Department in Liaoning Province of China (No.L2014048)
文摘FeCoCrNiA1Bx（x=0, 0.25, 0.50, 0.75） coatings were prepared by laser cladding to study the effects of boron on the structure and properties of high-entropy alloys coatings. The microstructure, microhardness, and wear resistance properties of the samples were investigated by scanning electron microscopy, X-ray diffraction, metallographic micro-hardness test, and friction wear test, respectively, and the mechanism of the wear behavior was also analyzed. The results showed that the high-entropy alloys consisted of BCC phase and eutectic structure, which contained FCC phase and M2B. With boron addition, the content of BCC phase increased while that of eutectic structure decreased. The wear resistance of the high-entropy coatings was considerably improved with increasing addition of horon, and accordingly, the FeCoCrNiAlB0.75 coating showed the best wear resistance.
基金grants from the National Natural Science Foundation of China (No. 81470270, No. 81700083, and No. 81870072)the National Key Research and Development Program of China (No. 2016YFC1304300)+2 种基金the Capital Clinical Features Applied Research and Achievement Promotion Project of Beijing, China (No. Z161100000516116)the Major projects of the National Natural Science Foundation of China (No. 81490534)the Fundamental Research Funds for the Central Universities of China (No. 3332018183).
文摘Despite recent advances in antibiotic use, in creasing numbers of human immunodeficiency virus (HIV)- negative patients with Pneumocystis jirovecii pneumonia (PCP) are being admitted to intensive care units (ICUs), and show a mortality rate of over 50%.[1] A high neutrophil count in bronchoalveolar lavage (BAL) fluid is a predictor of poor prognosis in HIV-negative patients with PCP.
基金financially supported by the National Natural Science Foundation of China(No.51301112, No.51401129)Natural Science Foundation of Liaoning Province of China(No.201602553 )+1 种基金China Postdoctoral Science Foundation(2015M571327)The Science Research Program of Education Department in Liaoning Province(No.L2014048)
文摘Yttria-stabilized zirconia（ YSZ） coatings were deposited by low pressure plasma spray（ LPPS） in 1.0× 10^4 Pa,1.5 × 10^4 Pa,and 2.5 × 10^4 Pa. Both in-flight particle diagnostic detected by DPV-2000 system and ANSYS-FLUENT software were used to study the connection between the parameters of flying particles and the coating formation,which might help to recognize the relationship between the operation parameters and the coatings quality. The results of simulation showed that particles in a lower spray pressure could achieve a higher velocity. The particle velocity was around 380 m/s at a distance of 35 cm from the nozzle at 1.0 × 10^4 Pa while only 300 m/s at 2.5 × 10^4 Pa in actual measurement.The results showed that the velocity of particles increased with decreasing the spray pressure,which might enhance the flattening rate of coatings and thereby decreased the porosity. The deposited YSZ coating with the lowest porosity can be gained under 1.0 × 10^4 Pa condition.
基金financially supported by National Natural Science Foundation of China (Nos.51301112 and 51401129)China Postdoctoral Science Foundation (2015M571327)+1 种基金the Natural Science Foundation of Liaoning Province(No.201602553)the Science Research Program of Education Department in Liaoning Province(No.L2014048)
文摘FeCoCrNiAl high entropy alloy coatings were prepared by supersonic air-plasma spraying.The coatings were post-treated by vacuum heat treatment at 600 and 900°C,and laser re-melting with 300 W,respectively,to study the influence of different treatments on the structure and properties of the coatings.The phase constitution,microstructure and microhardness of the coatings after treatments were investigated using X-ray diffraction,scanning electron microscopy and energy dispersive spectrometry.Results showed that the as-sprayed coatings consisted of pure metal and Fe-Cr.The AlNi;phase was obtained after the vacuum heat treatment process.A body-centered cubic structure with less AlNi;could be found in the coating after the laser re-melting process.The average hardness values of the as-sprayed coating and the coatings with two different temperature vacuum heat treatments and with laser re-melting were 177,227,266 and 682 HV,respectively.This suggests that the vacuum heat treatment promoted the alloying process of the coatings,and contributed to the enhancement of the coating wear resistance.The laser re-melted coating showed the best wear resistance.
基金the grants from the National Natural Science Foundation of China (Nos.1470270and 81401629) the National Key Research and Development Program ofChina (No.2016YFC1304300)the Capital Clinical Features Applied Research and Achievement Promotion Project of Beijing, China (No.Z 161100000516116).
文摘To the Editor:Following H7N9infection,the host lymphocyte immunity plays an antiviral role.[l] Furthermore,low frequencies of T-cells correlate with disease severity.[2]Herein,we present a H7N9-infected patient with life-threatening lymphopenia (only 0.06× 10^9/L in the peripheral blood),which we have barely ever seen before.We also measured the proportidns of T-cell subpopulations in the blood and bronchoalveolar lavage fluid (BALF).
基金The authors thank the National Natural Science Foundation of China for their financial support under contract No.51801210.
文摘To verify the microalloying function and segregation behavior of trace Mg at grain boundary in steel,the 2.25Cr–1Mo steel doped with 0.056%P containing different Mg contents was refined with a vacuum-induction furnace.The effects of trace Mg addition on the temper embrittlement susceptibility of 2.25Cr–1Mo steel were studied by step-cooling test and the segregation behavior of Mg at grain boundary was explored by Auger electron spectroscopy.It is shown that P-induced temper embrittlement susceptibility can be reduced after subjecting to step-cooling treatment with trace Mg addition,mainly benefited from the segregation of Mg at grain boundary.This segregation can decrease the segregation amounts of P and S,especially for P,and increase the grain boundary cohesion,reducing the adverse effect on temper embrittlement caused by P and S.
