Surface acoustic wave(SAW) devices have been utilized for the sensing of chemical and biological phenomena in microscale for the past few decades. In this study, SAW device was fabricated by electrospinning poly(vinyl...Surface acoustic wave(SAW) devices have been utilized for the sensing of chemical and biological phenomena in microscale for the past few decades. In this study, SAW device was fabricated by electrospinning poly(vinylidenefluoride-co-trifluoroethylene)(P(VDF-TrFE)) incorporated with zinc oxide(ZnO) nanoparticles over the delay line area of the SAW device. The morphology, composition, and crystallinity of P(VDF-TrFE)/ZnO nanocomposites were investigated. After measurement of SAW frequency response, it was found that the insertion loss of the SAW devices incorporated with ZnO nanoparticles was much less than that of the neat polymer-deposited device. The fabricated device was expected to be used in acoustic biosensors to detect and quantify the cell proliferation in cell culture systems.展开更多
Piezoelectric materials that generate electrical signals in response to mechanical strain can be used in tissue engineering to stimulate cell proliferation. Poly （vinylidene fluoride-trifluoroethylene） （P（VDF-TrF...Piezoelectric materials that generate electrical signals in response to mechanical strain can be used in tissue engineering to stimulate cell proliferation. Poly （vinylidene fluoride-trifluoroethylene） （P（VDF-TrFE））, a piezoelectric polymer, is widely used in biomaterial applications. We hypothesized that incorporation of zinc oxide （ZnO） nanoparticles into the P（VDF-TrFE） matrix could promote adhesion, migration, and proliferation of cells, as well as blood vessel formation （angiogenesis）. In this study, we fabricated and comprehensively characterized a novel electrospun P（VDF-TrFE）/ZnO nanocomposite tissue engineering scaffold. We analyzed the morphological features of the polymeric matrix by scanning electron microscopy, and utilized Fourier transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry to examine changes in the crystalline phases of the copolymer due to addition of the nanoparticles. We detected no or minimal adverse effects of the biomaterials with regard to blood compatibility in vitro, biocompatibility, and cytotoxicity, indicating that P（VDF-TrFE）/ZnO nanocomposite scaffolds are suitable for tissue engineering applications. Interestingly, human mesenchymal stem cells （hMSCs） and human umbilical vein endothelial cells cultured on the nanocomposite scaffolds exhibited higher cell viability, adhesion, and proliferation compared to cells cultured on tissue culture plates or neat P（VDF-TrFE） scaffolds. Nanocomposite scaffolds implanted into rats with or without hMSCs did not elicit immunological responses, as assessed by macroscopic analysis and histology. Importantly, nanocomposite scaffolds promoted angiogenesis, which was increased in scaffolds pre-seeded with hMSCs. Overall, our results highlight the potential of these novel P（VDF-TrFE）/ZnO nanocomposites for use in tissue engineering, due to their biocompatibility and ability to promote cell adhesion and angiogenesis.展开更多
Cellulose is a linear biopolymer which is composed of nanofibrils,thus having a large surface area.This low-cost,low-density,high-specific-surface-area,easily processable polymer is found in nature in the form of plan...Cellulose is a linear biopolymer which is composed of nanofibrils,thus having a large surface area.This low-cost,low-density,high-specific-surface-area,easily processable polymer is found in nature in the form of plants,bacteria and tunicates.Cellulose has outstanding characteristics including low cytotoxicity,biocompatibility,good mechanical properties,high chemical stability,and cost effectiveness which make them suitable candidates for biomedical applications.The ma-nipulation of cellulose at nanoscale resulted in nanocellulose having exceptional physicochem-ical properties.Therefore,cellulose nanocomposite is a fascinating area of research which has applications in biomedical fields like wound healing,bone tissue engineering,three dimensional printing,drug carriers,medical implants etc.This review is mainly focused on the developments in the generation of cellulose nanocomposites and their potential applications in the biomedical field.展开更多
基金the Agence Nationale de la Recherche for the financial support (ANR-12-BS09021)the Department of Biotechnology (DBT), Government of India, New Delhi, for the financial support through MSUB IPLSARE Program (BT/PR4800/INF/22/152/2012)
文摘Surface acoustic wave(SAW) devices have been utilized for the sensing of chemical and biological phenomena in microscale for the past few decades. In this study, SAW device was fabricated by electrospinning poly(vinylidenefluoride-co-trifluoroethylene)(P(VDF-TrFE)) incorporated with zinc oxide(ZnO) nanoparticles over the delay line area of the SAW device. The morphology, composition, and crystallinity of P(VDF-TrFE)/ZnO nanocomposites were investigated. After measurement of SAW frequency response, it was found that the insertion loss of the SAW devices incorporated with ZnO nanoparticles was much less than that of the neat polymer-deposited device. The fabricated device was expected to be used in acoustic biosensors to detect and quantify the cell proliferation in cell culture systems.
文摘Piezoelectric materials that generate electrical signals in response to mechanical strain can be used in tissue engineering to stimulate cell proliferation. Poly （vinylidene fluoride-trifluoroethylene） （P（VDF-TrFE））, a piezoelectric polymer, is widely used in biomaterial applications. We hypothesized that incorporation of zinc oxide （ZnO） nanoparticles into the P（VDF-TrFE） matrix could promote adhesion, migration, and proliferation of cells, as well as blood vessel formation （angiogenesis）. In this study, we fabricated and comprehensively characterized a novel electrospun P（VDF-TrFE）/ZnO nanocomposite tissue engineering scaffold. We analyzed the morphological features of the polymeric matrix by scanning electron microscopy, and utilized Fourier transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry to examine changes in the crystalline phases of the copolymer due to addition of the nanoparticles. We detected no or minimal adverse effects of the biomaterials with regard to blood compatibility in vitro, biocompatibility, and cytotoxicity, indicating that P（VDF-TrFE）/ZnO nanocomposite scaffolds are suitable for tissue engineering applications. Interestingly, human mesenchymal stem cells （hMSCs） and human umbilical vein endothelial cells cultured on the nanocomposite scaffolds exhibited higher cell viability, adhesion, and proliferation compared to cells cultured on tissue culture plates or neat P（VDF-TrFE） scaffolds. Nanocomposite scaffolds implanted into rats with or without hMSCs did not elicit immunological responses, as assessed by macroscopic analysis and histology. Importantly, nanocomposite scaffolds promoted angiogenesis, which was increased in scaffolds pre-seeded with hMSCs. Overall, our results highlight the potential of these novel P（VDF-TrFE）/ZnO nanocomposites for use in tissue engineering, due to their biocompatibility and ability to promote cell adhesion and angiogenesis.
文摘Cellulose is a linear biopolymer which is composed of nanofibrils,thus having a large surface area.This low-cost,low-density,high-specific-surface-area,easily processable polymer is found in nature in the form of plants,bacteria and tunicates.Cellulose has outstanding characteristics including low cytotoxicity,biocompatibility,good mechanical properties,high chemical stability,and cost effectiveness which make them suitable candidates for biomedical applications.The ma-nipulation of cellulose at nanoscale resulted in nanocellulose having exceptional physicochem-ical properties.Therefore,cellulose nanocomposite is a fascinating area of research which has applications in biomedical fields like wound healing,bone tissue engineering,three dimensional printing,drug carriers,medical implants etc.This review is mainly focused on the developments in the generation of cellulose nanocomposites and their potential applications in the biomedical field.
