Laser speckle imaging has been widely used for in-vivo visualization of blood perfusion in biological tissues.However,existing laser speckle imaging techniques suffer from limited quantification accuracy and spatial r...Laser speckle imaging has been widely used for in-vivo visualization of blood perfusion in biological tissues.However,existing laser speckle imaging techniques suffer from limited quantification accuracy and spatial resolution.Here we re-port a novel design and implementation of a powerful laser speckle imaging platform to solve the two critical limitations.The core technique of our platform is a combination of line scan confocal microscopy with laser speckle autocorrelation imaging,which is termed Line Scan Laser Speckle Autocorrelation Imaging(LS-LSAI).The technical advantages of LS-LSAI include high spatial resolution(~4.4μm)for visualizing and quantifying blood flow in microvessels,as well as video-rate imaging speed for tracing dynamic flow.展开更多
基金supports from Ministry of Education-Singapore(MOE2019-T2-2-094,R-397-000-327-114).
文摘Laser speckle imaging has been widely used for in-vivo visualization of blood perfusion in biological tissues.However,existing laser speckle imaging techniques suffer from limited quantification accuracy and spatial resolution.Here we re-port a novel design and implementation of a powerful laser speckle imaging platform to solve the two critical limitations.The core technique of our platform is a combination of line scan confocal microscopy with laser speckle autocorrelation imaging,which is termed Line Scan Laser Speckle Autocorrelation Imaging(LS-LSAI).The technical advantages of LS-LSAI include high spatial resolution(~4.4μm)for visualizing and quantifying blood flow in microvessels,as well as video-rate imaging speed for tracing dynamic flow.
