Recent developments in organoid culture technologies have made it possible to closely recapitulate intrinsic characteristics of different tissues under in vitro conditions.These organoids act as a translational bridge...Recent developments in organoid culture technologies have made it possible to closely recapitulate intrinsic characteristics of different tissues under in vitro conditions.These organoids act as a translational bridge between the traditional 2D/3D cultures and the in vivo models for studying the tissue development processes,disease modeling,and drug screening.Matrigel and tissue-specific extracellular matrix have been shown to support organoid development,efficiently;however,their chemically undefined nature,non-tunable properties,and associated batch-to-batch variations often limit reproducibility of the assembly process.In this regard,chemically defined platforms offer wider opportunities to optimize and recreate tissue-specific microenvironment.The present review delineates the current research trends in this sphere,focusing on material perspective and the target tissues(e.g.,neural,liver,pancreatic,renal,and intestinal).The review winds up with a discussion on the current limitations and future perspective to provide a basis for future research.展开更多
文摘Recent developments in organoid culture technologies have made it possible to closely recapitulate intrinsic characteristics of different tissues under in vitro conditions.These organoids act as a translational bridge between the traditional 2D/3D cultures and the in vivo models for studying the tissue development processes,disease modeling,and drug screening.Matrigel and tissue-specific extracellular matrix have been shown to support organoid development,efficiently;however,their chemically undefined nature,non-tunable properties,and associated batch-to-batch variations often limit reproducibility of the assembly process.In this regard,chemically defined platforms offer wider opportunities to optimize and recreate tissue-specific microenvironment.The present review delineates the current research trends in this sphere,focusing on material perspective and the target tissues(e.g.,neural,liver,pancreatic,renal,and intestinal).The review winds up with a discussion on the current limitations and future perspective to provide a basis for future research.
