Chaetoceros Ehrenberg is one of the most diverse genera of planktonic diatoms.The species in section Chaetoceros are characterized by cells and setae having numerous chloroplasts and being widely distributed.However,t...Chaetoceros Ehrenberg is one of the most diverse genera of planktonic diatoms.The species in section Chaetoceros are characterized by cells and setae having numerous chloroplasts and being widely distributed.However,the delimitations of some species are problematic because of limited morphological information in the classical descriptions.Monoclonal strains of the section Chaetoceros were established,morphological features were studied using light and electron microscopy,and the hypervariable D 1-D 3 region of the nuclear ribosomal large subunit gene was sequenced to address phylogenetic relationships.Fifteen species belonging to the section Chaetoceros were recorded,including two new species,C.hainanensis sp.nov.and C.tridiscus sp.nov.Chaetoceros hainanensis was characterized by straight chains,narrowly lanceolate to hexagonal apertures,sibling setae diverging in nearly right angles,stipule-shaped spines on terminal setae and arrowhead-shaped spines on intercalary setae.C.tridiscus had short straight chains,narrowly lanceolate apertures,arrowhead-shaped spines and circular poroids arranged in a grid pattern on terminal and intercalary setae.The phylogenetic analyses revealed six groups formed by 19 species within the section Chaetoceros,which was found to be monophyletic.The subdivision of the section is still not well understood.The morphological characters within each group varied considerably and molecular information on more species are needed to enrich the phylogenetic profiling.展开更多
Diatoms are unicellular algae enclosed in intricate bio-silicified walls with repetitive nanostructures in a size range which makes them potentially relevant for a broad spectrum of industrial applications. How to opt...Diatoms are unicellular algae enclosed in intricate bio-silicified walls with repetitive nanostructures in a size range which makes them potentially relevant for a broad spectrum of industrial applications. How to optimize the nano-scale structures of the frustule for utilization of diatoms in nanotechnology is one of the technological challenges for these applications. Light is one of the most important abiotic factors for algal photosynthetic growth, and the frustule may play an important role in mediating light for these biological functions, as well as being central for its nano-technological applications. In this study we tested the influence of light quality on the nanostructure of the frustule of Coscinodiscus granii and compared this to growth rate response. The results showed that colored light （red, yellow, green and blue） at 300μmol photons m-2-s-1 resulted in a statistically significant change in nanostructure compared to white light. Green light at 100 μmol photon m-2.s-1 led to a significant decrease in mean frustule diameter and mean foramen diameter. Numerical simulations confirmed that the morphological changes obtained were sufficient to induce clear differences in the photonics properties of the frustule. The wavelength had no effect on the growth rate at high light intensity （300 μmol photons m-2.s-1）. However, at 100 μmol photons m-2.s-1, yellow, red-orange and green light resulted in significantly lower maximum growth rates than the other wavelengths. This response of the frustule structure to different light treatment indicates the possibility of a light-based frustule nanostructure manipulation method, which is simple and environmentally friendly.展开更多
基金Supported by the Joint Fund of National Natural Science Foundation of China and Chinese Shandong Province(No.U 2106205)the National Natural Science Foundation of China(No.32170206)the National Key Research and Development Program of China(No.2022YFC3105201)。
文摘Chaetoceros Ehrenberg is one of the most diverse genera of planktonic diatoms.The species in section Chaetoceros are characterized by cells and setae having numerous chloroplasts and being widely distributed.However,the delimitations of some species are problematic because of limited morphological information in the classical descriptions.Monoclonal strains of the section Chaetoceros were established,morphological features were studied using light and electron microscopy,and the hypervariable D 1-D 3 region of the nuclear ribosomal large subunit gene was sequenced to address phylogenetic relationships.Fifteen species belonging to the section Chaetoceros were recorded,including two new species,C.hainanensis sp.nov.and C.tridiscus sp.nov.Chaetoceros hainanensis was characterized by straight chains,narrowly lanceolate to hexagonal apertures,sibling setae diverging in nearly right angles,stipule-shaped spines on terminal setae and arrowhead-shaped spines on intercalary setae.C.tridiscus had short straight chains,narrowly lanceolate apertures,arrowhead-shaped spines and circular poroids arranged in a grid pattern on terminal and intercalary setae.The phylogenetic analyses revealed six groups formed by 19 species within the section Chaetoceros,which was found to be monophyletic.The subdivision of the section is still not well understood.The morphological characters within each group varied considerably and molecular information on more species are needed to enrich the phylogenetic profiling.
文摘Diatoms are unicellular algae enclosed in intricate bio-silicified walls with repetitive nanostructures in a size range which makes them potentially relevant for a broad spectrum of industrial applications. How to optimize the nano-scale structures of the frustule for utilization of diatoms in nanotechnology is one of the technological challenges for these applications. Light is one of the most important abiotic factors for algal photosynthetic growth, and the frustule may play an important role in mediating light for these biological functions, as well as being central for its nano-technological applications. In this study we tested the influence of light quality on the nanostructure of the frustule of Coscinodiscus granii and compared this to growth rate response. The results showed that colored light （red, yellow, green and blue） at 300μmol photons m-2-s-1 resulted in a statistically significant change in nanostructure compared to white light. Green light at 100 μmol photon m-2.s-1 led to a significant decrease in mean frustule diameter and mean foramen diameter. Numerical simulations confirmed that the morphological changes obtained were sufficient to induce clear differences in the photonics properties of the frustule. The wavelength had no effect on the growth rate at high light intensity （300 μmol photons m-2.s-1）. However, at 100 μmol photons m-2.s-1, yellow, red-orange and green light resulted in significantly lower maximum growth rates than the other wavelengths. This response of the frustule structure to different light treatment indicates the possibility of a light-based frustule nanostructure manipulation method, which is simple and environmentally friendly.
