Methicillin-resistant </span><i><span style="font-family:Verdana;">Staphylococcus aureus </span></i><span style="font-family:Verdana;">(MRSA) infection is a glob...Methicillin-resistant </span><i><span style="font-family:Verdana;">Staphylococcus aureus </span></i><span style="font-family:Verdana;">(MRSA) infection is a global health concern that has caused severe health threats over the past decade. Leaves extract of </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">has been proven previously as an anti MRSA agent. Proteomics provide a technique that used to analyze the differential of protein expression profile between untreated and treated MRSA with subinhibitory concentrations of acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span><span style="font-family:Verdana;">leaves. This study aims to determine the optimum parameter for analysis of protein expression profile using two-dimension gels electrophoresis (2-DE) for MRSA protein after treatment with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllu</span></i></span><i><span style="font-family:Verdana;">m </span></i><span style="font-family:Verdana;">leaves. Comparison of the Protein Expression Profile (PEP) between the untreated and treated MRSA was analyzed using PDQuest software. The optimum condition for MRSA protein treated with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">leaves to produce the best resolution with greater spot distribution was as follows: 100 μg volume of MRSA protein that loaded after passive rehydration then was run until reaching 25 kVrhs during IEF using 17 cm IPG strip within ranges of pH 4 - 7. Analysis of protein expression from the 2-DE gel map shows that 9 protein spots up-regulated and 41 protein spots were down-regulated with more than 2-fold differences (p < 0.05). This preliminary study on the PEP of MRSA treated with acetone extract of </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">leave may provide an insight into the antimicrobial mechanism, which could lead to the identification of target protein for future novel therapeutic development against MRSA infections.展开更多
文摘Methicillin-resistant </span><i><span style="font-family:Verdana;">Staphylococcus aureus </span></i><span style="font-family:Verdana;">(MRSA) infection is a global health concern that has caused severe health threats over the past decade. Leaves extract of </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">has been proven previously as an anti MRSA agent. Proteomics provide a technique that used to analyze the differential of protein expression profile between untreated and treated MRSA with subinhibitory concentrations of acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span><span style="font-family:Verdana;">leaves. This study aims to determine the optimum parameter for analysis of protein expression profile using two-dimension gels electrophoresis (2-DE) for MRSA protein after treatment with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllu</span></i></span><i><span style="font-family:Verdana;">m </span></i><span style="font-family:Verdana;">leaves. Comparison of the Protein Expression Profile (PEP) between the untreated and treated MRSA was analyzed using PDQuest software. The optimum condition for MRSA protein treated with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">leaves to produce the best resolution with greater spot distribution was as follows: 100 μg volume of MRSA protein that loaded after passive rehydration then was run until reaching 25 kVrhs during IEF using 17 cm IPG strip within ranges of pH 4 - 7. Analysis of protein expression from the 2-DE gel map shows that 9 protein spots up-regulated and 41 protein spots were down-regulated with more than 2-fold differences (p < 0.05). This preliminary study on the PEP of MRSA treated with acetone extract of </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">leave may provide an insight into the antimicrobial mechanism, which could lead to the identification of target protein for future novel therapeutic development against MRSA infections.
