Two relatively pure polysaccharides H3-a1 and H3-b1 had been isolated from the brown seaweed Hy-droclathrus clathratus. They were characterized by HPLC, ultraviolet scanning, gas chromatography, infrared spectroscopy ...Two relatively pure polysaccharides H3-a1 and H3-b1 had been isolated from the brown seaweed Hy-droclathrus clathratus. They were characterized by HPLC, ultraviolet scanning, gas chromatography, infrared spectroscopy and elemental analysis, and shown to be two different sulfated polysaccharides with different monosaccharide content, but both with high relative molecular mass. They contained some proteins and uronic acid respectively. The sulfate content and bioactivity of these polysaccha-rides varied during purification. The fractions derived from the hot water extract also exhibited low anticoagulant effect. This is the first time that the antiherpetic and anticoagulant activities were evaluated for the polysaccharides from the Hong Kong brown seaweed Hydroclathrus clathratus.展开更多
基金Supported by the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. CUHK4367/01M)a Direct Grant from the Chinese University of Hong Kong
文摘Two relatively pure polysaccharides H3-a1 and H3-b1 had been isolated from the brown seaweed Hy-droclathrus clathratus. They were characterized by HPLC, ultraviolet scanning, gas chromatography, infrared spectroscopy and elemental analysis, and shown to be two different sulfated polysaccharides with different monosaccharide content, but both with high relative molecular mass. They contained some proteins and uronic acid respectively. The sulfate content and bioactivity of these polysaccha-rides varied during purification. The fractions derived from the hot water extract also exhibited low anticoagulant effect. This is the first time that the antiherpetic and anticoagulant activities were evaluated for the polysaccharides from the Hong Kong brown seaweed Hydroclathrus clathratus.
