An artificial potential field method based on global path guidance(G-APF)is proposed for target unreachability and local minima problems of the conventional artificial potential field(APF)method in complex environment...An artificial potential field method based on global path guidance(G-APF)is proposed for target unreachability and local minima problems of the conventional artificial potential field(APF)method in complex environments with dynamic obstacles.First,for the target unreachability problem,the global path attraction is added to the APF;second,an obstacle detection optimisation method is proposed and the optimal virtual target point is selected by setting the evaluation function to improve the local minima problem;finally,based on the obstacle detection optimisation method,the gravitational and repulsive processes are improved so that the path can pass through the narrow channel smoothly and remain collision-free.Experiments show that the method optimises 40.8%of the total path corners,reduces 81.8%of the number of path oscillations,and shortens 4.3%of the path length in Map 1.It can be applied to the vehicle obstacle avoidance path planning problem in complex environments with dynamic obstacles.展开更多
Metasurface biosensors have become the core label-free and rapid-detection technology in bioanalysis.Lung cancer and brain cancer are the first leading causes of cancer death among adults and adolescents,respectively,...Metasurface biosensors have become the core label-free and rapid-detection technology in bioanalysis.Lung cancer and brain cancer are the first leading causes of cancer death among adults and adolescents,respectively,where poor early diagnosis results from expensive detection costs and time consumption.To tackle the above problems,here,we introduce a terahertzdomain metasurface biosensor for cancer diagnosis,relying on a perfectly symmetrical periodic surface structure,which significantly exhibits polarization-insensitivity at 2.05 THz and the high-sensitivity of 504 GHz/RIU(RIU=refractive index unit).According to the frequency shifts and transmittance variations,four cell types are successfully distinguished from each other.The minimum number of cells is required for thousands of cells to display the differences of spectra,which is 1/30 of clinical methods.Furthermore,the results were consistent with pathological results(the gold standard in clinic)by Gaussian curve fitting.The proposed biosensor has really achieved the characterization of cells in normal and cancerous state.This detection strategy dramatically reduced the cost of detection by reuse and time consumption was reduced to 1/20 of the pathology testing.In addition,it is flexible to set samples and easy to realize automatic operation due to the great polarization-insensitivity of the proposed biosensor,which can further reduce labor costs in the future.It is envisioned that the proposed biosensor will present immense potential in the fields of cancer detection,distinguishing different cancers,identifying primary lesion cancer or metastatic cancer.展开更多
Background The Jiangmen underground neutrino observatory(JUNO)is a new generation of long-term operation neutrino experimental platform under construction in Jiangmen,Guangdong province of southern China.The underwate...Background The Jiangmen underground neutrino observatory(JUNO)is a new generation of long-term operation neutrino experimental platform under construction in Jiangmen,Guangdong province of southern China.The underwater frontend and readout electronics(F&R electronics)are placed nearby the 20-inch PMTs and readout current signals from three PMTs.The electronics are sealed in a stainless-steel underwater box to keep them dry,and temperature control of the electronics plays a significant role in their reliability.Methods A conductive cooling structure based on the passive cooling method is designed for the 20-inch PMT F&R electronics.Both numerical simulation and experimental tests were performed to evaluate the cooling structures'thermal performance.Results The results show that the case temperature of the respective chip for 20-inch PMT F&R electronics is less than 30℃in ambient still water.Conclusion The case temperature of the respective chip meets the strict temperature requirement for the F&R electronics.The JUNO collaboration has adopted this cooling structure for the 20-inch PMT readout electronics.展开更多
Metastasis and metabolic disorders contribute to most cancer deaths and are potential drug targets in cancer treatment. However, corresponding drugs inevitably induce myeloid suppression and gastrointestinal toxicity....Metastasis and metabolic disorders contribute to most cancer deaths and are potential drug targets in cancer treatment. However, corresponding drugs inevitably induce myeloid suppression and gastrointestinal toxicity. Here, we report a nonpharmaceutical and noninvasive electromagnetic intervention technique that exhibited long-term inhibition of cancer cells. Firstly, we revealed that optical radiation at the specific wavelength of 3.6 μm (i.e., 83 THz) significantly increased binding affinity between DNA and histone via molecular dynamics simulations, providing a theoretical possibility for THz modulation- (THM-) based cancer cell intervention. Subsequent cell functional assays demonstrated that low-power 3.6 μm THz wave could successfully inhibit cancer cell migration by 50% and reduce glycolysis by 60%. Then, mRNA sequencing and assays for transposase-accessible chromatin using sequencing (ATAC-seq) indicated that low-power THM at 3.6 μm suppressed the genes associated with glycolysis and migration by reducing the chromatin accessibility of certain gene loci. Furthermore, THM at 3.6 μm on HCT-116 cancer cells reduced the liver metastasis by 60% in a metastatic xenograft mouse model by splenic injection, successfully validated the inhibition of cancer cell migration by THM in vivo. Together, this work provides a new paradigm for electromagnetic irradiation-induced epigenetic changes and represents a theoretical basis for possible innovative therapeutic applications of THM as the future of cancer treatments.展开更多
基金supported by the Zhejiang Province New Young Talent Plan Project in 2022 under Grant No.2022R431B021。
文摘An artificial potential field method based on global path guidance(G-APF)is proposed for target unreachability and local minima problems of the conventional artificial potential field(APF)method in complex environments with dynamic obstacles.First,for the target unreachability problem,the global path attraction is added to the APF;second,an obstacle detection optimisation method is proposed and the optimal virtual target point is selected by setting the evaluation function to improve the local minima problem;finally,based on the obstacle detection optimisation method,the gravitational and repulsive processes are improved so that the path can pass through the narrow channel smoothly and remain collision-free.Experiments show that the method optimises 40.8%of the total path corners,reduces 81.8%of the number of path oscillations,and shortens 4.3%of the path length in Map 1.It can be applied to the vehicle obstacle avoidance path planning problem in complex environments with dynamic obstacles.
基金the National Natural Science Foundation of China(Nos.12225511,T2241002,and 61988102,)and XPLORER PRIZE of C.C.
文摘Metasurface biosensors have become the core label-free and rapid-detection technology in bioanalysis.Lung cancer and brain cancer are the first leading causes of cancer death among adults and adolescents,respectively,where poor early diagnosis results from expensive detection costs and time consumption.To tackle the above problems,here,we introduce a terahertzdomain metasurface biosensor for cancer diagnosis,relying on a perfectly symmetrical periodic surface structure,which significantly exhibits polarization-insensitivity at 2.05 THz and the high-sensitivity of 504 GHz/RIU(RIU=refractive index unit).According to the frequency shifts and transmittance variations,four cell types are successfully distinguished from each other.The minimum number of cells is required for thousands of cells to display the differences of spectra,which is 1/30 of clinical methods.Furthermore,the results were consistent with pathological results(the gold standard in clinic)by Gaussian curve fitting.The proposed biosensor has really achieved the characterization of cells in normal and cancerous state.This detection strategy dramatically reduced the cost of detection by reuse and time consumption was reduced to 1/20 of the pathology testing.In addition,it is flexible to set samples and easy to realize automatic operation due to the great polarization-insensitivity of the proposed biosensor,which can further reduce labor costs in the future.It is envisioned that the proposed biosensor will present immense potential in the fields of cancer detection,distinguishing different cancers,identifying primary lesion cancer or metastatic cancer.
基金This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDA100102).
文摘Background The Jiangmen underground neutrino observatory(JUNO)is a new generation of long-term operation neutrino experimental platform under construction in Jiangmen,Guangdong province of southern China.The underwater frontend and readout electronics(F&R electronics)are placed nearby the 20-inch PMTs and readout current signals from three PMTs.The electronics are sealed in a stainless-steel underwater box to keep them dry,and temperature control of the electronics plays a significant role in their reliability.Methods A conductive cooling structure based on the passive cooling method is designed for the 20-inch PMT F&R electronics.Both numerical simulation and experimental tests were performed to evaluate the cooling structures'thermal performance.Results The results show that the case temperature of the respective chip for 20-inch PMT F&R electronics is less than 30℃in ambient still water.Conclusion The case temperature of the respective chip meets the strict temperature requirement for the F&R electronics.The JUNO collaboration has adopted this cooling structure for the 20-inch PMT readout electronics.
基金This work was financially supported by the China Postdoctoral Science Foundation(2020M673689)the National Natural Science Foundation of China(82073390,81702314,61905286,and T2241002)+2 种基金the Beijing Science and Technology Nova Program(Z191100001119128)the Beijing Municipal Science and Technology Project(Z191100006619081)the National Defense Technology Innovation Special Zone.CC acknowledges the support of X-plore Prize.
文摘Metastasis and metabolic disorders contribute to most cancer deaths and are potential drug targets in cancer treatment. However, corresponding drugs inevitably induce myeloid suppression and gastrointestinal toxicity. Here, we report a nonpharmaceutical and noninvasive electromagnetic intervention technique that exhibited long-term inhibition of cancer cells. Firstly, we revealed that optical radiation at the specific wavelength of 3.6 μm (i.e., 83 THz) significantly increased binding affinity between DNA and histone via molecular dynamics simulations, providing a theoretical possibility for THz modulation- (THM-) based cancer cell intervention. Subsequent cell functional assays demonstrated that low-power 3.6 μm THz wave could successfully inhibit cancer cell migration by 50% and reduce glycolysis by 60%. Then, mRNA sequencing and assays for transposase-accessible chromatin using sequencing (ATAC-seq) indicated that low-power THM at 3.6 μm suppressed the genes associated with glycolysis and migration by reducing the chromatin accessibility of certain gene loci. Furthermore, THM at 3.6 μm on HCT-116 cancer cells reduced the liver metastasis by 60% in a metastatic xenograft mouse model by splenic injection, successfully validated the inhibition of cancer cell migration by THM in vivo. Together, this work provides a new paradigm for electromagnetic irradiation-induced epigenetic changes and represents a theoretical basis for possible innovative therapeutic applications of THM as the future of cancer treatments.
