Repairing large-area soft tissue defects caused by traumas is a major surgical challenge.Developing multifunctional scaffolds with suitable scalability and favorable cellular response is crucial for soft tissue regene...Repairing large-area soft tissue defects caused by traumas is a major surgical challenge.Developing multifunctional scaffolds with suitable scalability and favorable cellular response is crucial for soft tissue regeneration.In this study,we developed an orthogonally woven three-dimensional(3D)nanofiber scaffold combining electrospinning,weaving,and modified gas-foaming technology.The developed orthogonally woven 3D nanofiber scaffold had a modular design and controlled fiber alignment.In vitro,the orthogonally woven 3D nanofiber scaffold exhibited adjustable mechanical properties,good cell compatibility,and easy drug loading.In vivo,for one thing,the implantation of an orthogonally woven 3D nanofiber scaffold in a full abdominal wall defect model demonstrated that extensive granulation tissue formation with enough mechanical strength could promote recovery of abdominal wall defects while reducing intestinal adhesion.Another result of diabetic wound repair experiments suggested that orthogonally woven 3D nanofiber scaffolds had a higher wound healing ratio,granulation tissue formation,collagen deposition,and re-epithelialization.Taken together,this novel orthogonally woven 3D nanofiber scaffold may provide a promising and effective approach for optimal soft tissue regeneration.展开更多
基金supported by the National Natural Science Foundation of China(grant no.82102334 to S.Chen,grant no.82171622 to L.Liu,grant no.81971832 to L.Yi)The Key Foundation of Zhejiang Provincial Natural Science Foundation(grant no.LZ22C100001 to S.C.)+1 种基金The Wenzhou Science and Technology Major Project(grant no.ZY2022026 to S.Chen)Wenzhou Science and Technology Project(grant no.ZY2023144 to Z.Huang).
文摘Repairing large-area soft tissue defects caused by traumas is a major surgical challenge.Developing multifunctional scaffolds with suitable scalability and favorable cellular response is crucial for soft tissue regeneration.In this study,we developed an orthogonally woven three-dimensional(3D)nanofiber scaffold combining electrospinning,weaving,and modified gas-foaming technology.The developed orthogonally woven 3D nanofiber scaffold had a modular design and controlled fiber alignment.In vitro,the orthogonally woven 3D nanofiber scaffold exhibited adjustable mechanical properties,good cell compatibility,and easy drug loading.In vivo,for one thing,the implantation of an orthogonally woven 3D nanofiber scaffold in a full abdominal wall defect model demonstrated that extensive granulation tissue formation with enough mechanical strength could promote recovery of abdominal wall defects while reducing intestinal adhesion.Another result of diabetic wound repair experiments suggested that orthogonally woven 3D nanofiber scaffolds had a higher wound healing ratio,granulation tissue formation,collagen deposition,and re-epithelialization.Taken together,this novel orthogonally woven 3D nanofiber scaffold may provide a promising and effective approach for optimal soft tissue regeneration.
