Fluorinated carbons CF_xhold the highest theoretical energy density(e.g.,2180 W h kg^(-1)when x=1)among all cathode materials of lithium primary batteries.However,the low conductivity and severe polarization limit it ...Fluorinated carbons CF_xhold the highest theoretical energy density(e.g.,2180 W h kg^(-1)when x=1)among all cathode materials of lithium primary batteries.However,the low conductivity and severe polarization limit it to achieve its theory.In this study,we design a new electrolyte,namely 1 M LiBF_(4)DMSO:DOL(1:9 vol.),achieving a high energy density in Li/CF_xprimary cells.The DMSO with a small molecular size and high donor number successfully solvates Li^(+)into a defined Li^(+)-solvation structure.Such solvated Li^(+)can intercalate into the large-spacing carbon layers and achieve an improved capacity.Consequently,when discharged to 1.0 V,the CF_(1.12)cathode demonstrates a specific capacity of 1944 m A h g^(-1)with a specific energy density of 3793 W h kg^(-1).This strategy demonstrates that designing the electrolyte is powerful in improving the electrochemical performance of CF_(x) cathode.展开更多
Fluorinated carbons(CFx)have been widely applied as lithium primary batteries due to their ultra-high energy density.It will be a great promise if CFx can be rechargeable.In this study,we rationally tune the C-F bond ...Fluorinated carbons(CFx)have been widely applied as lithium primary batteries due to their ultra-high energy density.It will be a great promise if CFx can be rechargeable.In this study,we rationally tune the C-F bond strength for the alkaline intercalated CFx via importing an electronegative weaker element K instead of Li.It forms a ternary phase K_(x)FC instead of two phases(LiF+C)in lithium-ion batteries.Meanwhile,we choose a large layer distance and more defects CFx,namely fluorinated soft carbon,to accommodate K.Thus,we enable CFx rechargeable as a potassium-ion battery cathode.In detail fluorinated soft carbon CF_(1.01) presents a reversible specific capacity of 339 mA h g^(-1)(797 Wh kg^(-1))in the 2nd cycle and maintains 330 mA h g^(-1)(726 Wh kg^(-1))in the 15th cycle.This study reveals the importance of tuning chemical bond stability using different alkaline ions to endow batteries with rechargeability.This work provides good references for focusing on developing reversible electrode materials from popular primary cell configurations.展开更多
Multiple failure modes tend to be identified in the reliability analysis of a redundant truss structure.This identification process involves updating the model for identifying the next potential failure members.Herein...Multiple failure modes tend to be identified in the reliability analysis of a redundant truss structure.This identification process involves updating the model for identifying the next potential failure members.Herein we intend to update the finite element model automatically in the identification process of failure modes and further perform the system reliability analysis efficiently.This study presents a framework that is implemented through the joint simulation of MATLAB and APDL and consists of three parts:reliability index of a single member,identification of dominant failure modes,and system-level reliability analysis for system reliability analysis of truss structures.Firstly,RSM(response surface method)combines with a constrained optimization model to calculate the reliability indices ofmembers.Then theβ-unzipping method is adopted to identify the dominant failuremodes,and the system function in MATLAB,as well as the EKILL command in APDL,is used to facilitate the automatic update of the finite element model and realize load-redistribution.Besides,the differential equivalence recursion algorithmis performed to approximate the reliability indices of failuremodes efficiently and accurately.Eventually,the PNET(probabilistic network evaluation technique)is used to calculate the joint failure probability as well as the system reliability index.Two illustrative examples demonstrate the accuracy and efficiency of the proposed system reliability analysis framework through comparison with corresponding references.展开更多
Three-dimensional(3 D)frameworks have received much attention as an effective modification strategy for next-generation high-energy-density lithium metal batteries.However,the top-growth mode of lithium(Li)on the 3 D ...Three-dimensional(3 D)frameworks have received much attention as an effective modification strategy for next-generation high-energy-density lithium metal batteries.However,the top-growth mode of lithium(Li)on the 3 D framework remains a tough challenge.To achieve a uniform bottom-up Li growth,a scheme involving Ag concentration gradient in 3 D PVDF framework(C-Ag/PVDF)is proposed.Ag nanoparticles with a concentration gradient induce an interface activity gradient in the 3 D framework,and this gradient feature is still maintained during the cycle.As a result,the C-Ag/PVDF framework delivers a long lifespan over 1800 h at a current density of 1 mA cm^(-2) with a capacity of 1 mAh cm^(-2),and shows an ultra-long life(>1300 h)even at a high current density of 4 mA cm^(-2) with a capacity of 4 mAh cm^(-2).The advantage of concentration gradient provides further insights into the optimal design of the 3 D framework for stable Li metal anode.展开更多
Pyrite FeS_(2)exhibits an ultrahigh energy density(1671 W·h·kg^(-1),for the reaction of FeS_(2)+4Li=Fe+2Li_(2)S)in secondary lithium-ion batteries,but its poor cycling stability,huge volume expansion,the shu...Pyrite FeS_(2)exhibits an ultrahigh energy density(1671 W·h·kg^(-1),for the reaction of FeS_(2)+4Li=Fe+2Li_(2)S)in secondary lithium-ion batteries,but its poor cycling stability,huge volume expansion,the shuttle effect of polysulfides,and slow kinetic properties limit its practical application.In this work,we synthesize a composite structure material CoS on FeS_(2)surface(FeSx@CoS,1<x≤2)by using a cobalt-containing MOF to improve its cycle stability.It is found that CoS inhibits the side reactions and adsorbs polysulfides.As a result,the modified FeS_(2)shows a higher discharge capacity of 577 mA·h·g^(-1)(919 W·h·kg^(-1))after 60 cycles than 484 mA·h·g^(-1)(778 W·h·kg^(-1))of bare pyrite FeS_(2).This efficient strategy provides a valuable step toward the realization of high cycling stability FeS_(2)cathode materials for secondary lithium-ion batteries and enriches the basic understanding of the influence of FeS_(2)interfacial stability on its electrochemical performances.展开更多
Three small molecule(SM)donors,namely B-T-CN,B-TT-CN and B-DTT-CN,with differentπconjugated bridges were synthesized in this research.Interestingly,with the conjugated fused rings of theπlinking bridge increasing,th...Three small molecule(SM)donors,namely B-T-CN,B-TT-CN and B-DTT-CN,with differentπconjugated bridges were synthesized in this research.Interestingly,with the conjugated fused rings of theπlinking bridge increasing,the SM HOMO levels exhibit a decline tendency with–5.27 eV for B-T-CN,–5.31 eV for B-TT-CN and–5.40 eV for B-DTT-CN.After blending the SM donors with the fullerene acceptor PC71BM,the all SM organic solar cells(OSCs)achieved high Vocs of 0.90 to 0.96 V.However,the phase separation morphology and molecule stacking are also unexpectedly changed together with the enhancement of conjugated degree ofπbridges,resulting in a lower power conversion efficiency(PCE)for the B-DTT-CN:PC71BM device.Our results demonstrate and provide a useful way to enhance OSC Voc and the morphology needs to be further optimized.展开更多
FeSO_(4) has the characteristics of low cost and theoretical high energy density(799 W·h·kg^(-1) with a two-electron reaction),which can meet the demand for next-generation lithium-ion batteries.Herein,FeSO_...FeSO_(4) has the characteristics of low cost and theoretical high energy density(799 W·h·kg^(-1) with a two-electron reaction),which can meet the demand for next-generation lithium-ion batteries.Herein,FeSO_(4) as a novel highperformance conversion-reaction type cathode is investigated.We use dopamine as a carbon coating source to increase its electronic conductivity.FeSO_(4)@C demonstrates a high reversible specific capacity(512 mA·h·g^(-1))and a superior cycling performance(482 mA·h·g^(-1) after 250 cycles).In addition,we further study its reaction mechanism.The FeSO_(4) is converted to Fe and Li2SO_(4) during lithium ion insertion and the Fe-Li_(2)SO_(4) grain boundaries further store additional lithium ions.Our findings are valuable in exploring other new conversion-type lithium ion battery cathodes.展开更多
Through the analysis on 211614 batches of samples from 35 categories of food safety supervision and inspection in Sichuan Province by Administration for Market Regulation of Sichuan Province in 2019,four suggestions w...Through the analysis on 211614 batches of samples from 35 categories of food safety supervision and inspection in Sichuan Province by Administration for Market Regulation of Sichuan Province in 2019,four suggestions were finally put forward.The analysis results showed that in 2019,the overall trend of food safety in Sichuan Province was stable and improved.A total of 30 categories of unqualified samples were detected,and a total of 12135 batches of unqualified samples were detected,with an average sample unqualified rate of 5.73%.Among them,the main problems of unqualified samples were as follows:the use of food additives beyond the scope and limit,accounting for 40.17%of the total number of unqualified samples,microbial contamination in food,accounting for 23.61%of the total unqualified samples,the residue index of agricultural and veterinary drugs in food not meeting the standard,accounting for 15.61%of the total unqualified samples,the quality index in food not meeting the standard,accounting for 8.51%of the total number of unqualified samples,and heavy metals and other pollutants in food exceeding the standard,accounting for 7.10%of the total unqualified samples.展开更多
There is a growing demand for time series data analysis in industry areas.Apache loTDB is a time series database designed for the Internet of Things(loT)with enhanced storage and I/O performance.With User-Defined Func...There is a growing demand for time series data analysis in industry areas.Apache loTDB is a time series database designed for the Internet of Things(loT)with enhanced storage and I/O performance.With User-Defined Functions(UDF)provided,computation for time series can be executed on Apache loTDB directly.To satisfy most of the common requirements in industrial time series analysis,we create a UDF library,loTDQ,on Apache loTDB.This library integrates stream computation functions on data quality analysis,data profiling,anomaly detection,data repairing,etc.loTDQ enables users to conduct a wide range of analyses,such as monitoring,error diagnosis,equipment reliability analysis.It provides a framework for users to examine loT time series with data quality problems.Experiments show that loTDQ keeps the same level of performance compared to mainstream alternatives,and shortens I/O consumption for Apache loTDB users.展开更多
Dendronized hyperbranched polymer （DHP） is a new kind of polymer, which combines the advantages of dendrimers and hy- perbranched polymers. In this work, two dendronized hyperbranched polymers, DttPG0 and DHPG1, wer...Dendronized hyperbranched polymer （DHP） is a new kind of polymer, which combines the advantages of dendrimers and hy- perbranched polymers. In this work, two dendronized hyperbranched polymers, DttPG0 and DHPG1, were successfully pre- pared through the simple ＂A3＋B2＂ type Sonogashira coupling reaction. The nonlinear optical （NLO） effects of DHPG0 and DItPG1, characterized by the d33 values, were 183 and 220 pm V-1 respectively, higher than those of their analogues of den- dronized polymers and dendrimers, thanks to the special topological structure. Also, the obtained polymers displayed excellent solubility, good processability, and high thermal stability.展开更多
Recently N-glycosylation was found to be required for the postembryonic development and metamorphosis of the holometabolous beetle Tribolium castaneum. However, the role of N-glycosylation in the development of hemime...Recently N-glycosylation was found to be required for the postembryonic development and metamorphosis of the holometabolous beetle Tribolium castaneum. However, the role of N-glycosylation in the development of hemimetabolous insects is unknown. To further elucidate the role of N-glycosylation in the development of insects, a functional characterization of the N-glycosylation-related genes (NGRGs) was performed in a model insect for hemimetabolous development, namely the brown planthopper Nilaparvata lugens. In this project, we report the effects of RNAi-mediated silencing of 15 NGRGs on the postembryonic development of N. lugens. Two major observations were made. First, interruption of the early steps of N-glycan processing led to a lethal phenotype during the transition from nymph to adult as was observed in T. castaneum. Second, we report here on an essential function for the α-1,6-fucosyl transferase in the ecdysis event of N. lugens between nymphal instars, since gene-silencing by RNAi led to failure of ecdysis and subsequent mortality of the treated insect.展开更多
In mammals,plant lectinshave been shown to possess immunomodulatory properties,acting in both the innate and adaptive immune system to modulate the production of mediators of the immune response,ultimately improving h...In mammals,plant lectinshave been shown to possess immunomodulatory properties,acting in both the innate and adaptive immune system to modulate the production of mediators of the immune response,ultimately improving host defences.At present,knowledge of immunomodulatory effects of plant lectins in insects is scarce.Treatment of insect cells with the Orysa sativa lectin,Orysata,was previously reported to induce cell aggregation,mimicking the immune process of encapsulation.In this project we investigated the potential immunomodulatory effects of this mannose-binding lectin using Drosophila melanogaster S2 cells.Identification of the Orysata binding partners on the surface of S2 cells through a pull-down assay andproteomic analysis revealed 221 putative interactors,several of which were immunity-related proteins.Subsequent qPCR analysis revealed the upregulation of Toll-and immune deficiency(IMD)-regulated antimicrobial peptides(Drs,Mtk,AttA,and Dpt)and signal transducers(Rel and Hid)belonging to the IMD pathway.In addition,the iron-binding protein Transferrin 3 was identified as a putative interactor for Orysata,and treatment of S2 cells with Orysata was shown to reduce the intracellular iron concentration.All together,we believe these results offer a new perspective on the effects by which plant lectins influence insect cells and contribute to the study of their immunomodulatory properties.展开更多
基金supported by the National Natural Science Foundation of China(Nos.52072061,22322903,12174162)the Natural Science Foundation of Sichuan,China(No.2023NSFSC1914)21C Innovation Laboratory,Contemporary Amperex Technology Ltd.by project No.21C-OP-202103。
文摘Fluorinated carbons CF_xhold the highest theoretical energy density(e.g.,2180 W h kg^(-1)when x=1)among all cathode materials of lithium primary batteries.However,the low conductivity and severe polarization limit it to achieve its theory.In this study,we design a new electrolyte,namely 1 M LiBF_(4)DMSO:DOL(1:9 vol.),achieving a high energy density in Li/CF_xprimary cells.The DMSO with a small molecular size and high donor number successfully solvates Li^(+)into a defined Li^(+)-solvation structure.Such solvated Li^(+)can intercalate into the large-spacing carbon layers and achieve an improved capacity.Consequently,when discharged to 1.0 V,the CF_(1.12)cathode demonstrates a specific capacity of 1944 m A h g^(-1)with a specific energy density of 3793 W h kg^(-1).This strategy demonstrates that designing the electrolyte is powerful in improving the electrochemical performance of CF_(x) cathode.
基金supported by the National Natural Science Foundation of China(52072061)21C Innovation Laboratory,Contemporary Amperex Technology Ltd.by project No.21C–OP–202103。
文摘Fluorinated carbons(CFx)have been widely applied as lithium primary batteries due to their ultra-high energy density.It will be a great promise if CFx can be rechargeable.In this study,we rationally tune the C-F bond strength for the alkaline intercalated CFx via importing an electronegative weaker element K instead of Li.It forms a ternary phase K_(x)FC instead of two phases(LiF+C)in lithium-ion batteries.Meanwhile,we choose a large layer distance and more defects CFx,namely fluorinated soft carbon,to accommodate K.Thus,we enable CFx rechargeable as a potassium-ion battery cathode.In detail fluorinated soft carbon CF_(1.01) presents a reversible specific capacity of 339 mA h g^(-1)(797 Wh kg^(-1))in the 2nd cycle and maintains 330 mA h g^(-1)(726 Wh kg^(-1))in the 15th cycle.This study reveals the importance of tuning chemical bond stability using different alkaline ions to endow batteries with rechargeability.This work provides good references for focusing on developing reversible electrode materials from popular primary cell configurations.
基金support from the National Key R&D Program of China(Grant Nos.2021YFB2600605,2021YFB2600600)the Overseas Scholar Program in the Hebei Province(C20190514)+1 种基金from the State Key Laboratory of Mechanical Behavior and System Safety of Traffic Engineering Structures Project(ZZ2020-20)from the Youth Foundation of Hebei Science and Technology Research Project(QN2018108).
文摘Multiple failure modes tend to be identified in the reliability analysis of a redundant truss structure.This identification process involves updating the model for identifying the next potential failure members.Herein we intend to update the finite element model automatically in the identification process of failure modes and further perform the system reliability analysis efficiently.This study presents a framework that is implemented through the joint simulation of MATLAB and APDL and consists of three parts:reliability index of a single member,identification of dominant failure modes,and system-level reliability analysis for system reliability analysis of truss structures.Firstly,RSM(response surface method)combines with a constrained optimization model to calculate the reliability indices ofmembers.Then theβ-unzipping method is adopted to identify the dominant failuremodes,and the system function in MATLAB,as well as the EKILL command in APDL,is used to facilitate the automatic update of the finite element model and realize load-redistribution.Besides,the differential equivalence recursion algorithmis performed to approximate the reliability indices of failuremodes efficiently and accurately.Eventually,the PNET(probabilistic network evaluation technique)is used to calculate the joint failure probability as well as the system reliability index.Two illustrative examples demonstrate the accuracy and efficiency of the proposed system reliability analysis framework through comparison with corresponding references.
基金supported by the Fundamental Research Funds for the Central Universities,China(ZYGX2019Z008)the National Natural Science Foundation of China(52072061)the Open Fund of the Key Laboratory for Renewable Energy,Chinese Academy of Sciences,Beijing Key Laboratory for New Energy Materials and Devices。
文摘Three-dimensional(3 D)frameworks have received much attention as an effective modification strategy for next-generation high-energy-density lithium metal batteries.However,the top-growth mode of lithium(Li)on the 3 D framework remains a tough challenge.To achieve a uniform bottom-up Li growth,a scheme involving Ag concentration gradient in 3 D PVDF framework(C-Ag/PVDF)is proposed.Ag nanoparticles with a concentration gradient induce an interface activity gradient in the 3 D framework,and this gradient feature is still maintained during the cycle.As a result,the C-Ag/PVDF framework delivers a long lifespan over 1800 h at a current density of 1 mA cm^(-2) with a capacity of 1 mAh cm^(-2),and shows an ultra-long life(>1300 h)even at a high current density of 4 mA cm^(-2) with a capacity of 4 mAh cm^(-2).The advantage of concentration gradient provides further insights into the optimal design of the 3 D framework for stable Li metal anode.
基金Project supported by the Fundamental Research Funds for the Central Universities,China(Grant No.ZYGX2019Z008)the National Natural Science Foundation of China(Grant No.52072061).
文摘Pyrite FeS_(2)exhibits an ultrahigh energy density(1671 W·h·kg^(-1),for the reaction of FeS_(2)+4Li=Fe+2Li_(2)S)in secondary lithium-ion batteries,but its poor cycling stability,huge volume expansion,the shuttle effect of polysulfides,and slow kinetic properties limit its practical application.In this work,we synthesize a composite structure material CoS on FeS_(2)surface(FeSx@CoS,1<x≤2)by using a cobalt-containing MOF to improve its cycle stability.It is found that CoS inhibits the side reactions and adsorbs polysulfides.As a result,the modified FeS_(2)shows a higher discharge capacity of 577 mA·h·g^(-1)(919 W·h·kg^(-1))after 60 cycles than 484 mA·h·g^(-1)(778 W·h·kg^(-1))of bare pyrite FeS_(2).This efficient strategy provides a valuable step toward the realization of high cycling stability FeS_(2)cathode materials for secondary lithium-ion batteries and enriches the basic understanding of the influence of FeS_(2)interfacial stability on its electrochemical performances.
基金This work was supported by National Natural Science Foundation of China(21801238)National Youth Thousand Program Project(R52A199Z11)+1 种基金CAS Pioneer Hundred Talents Program B(Y92A010Q10)Organic Semiconductor Center of Chongqing Institute of Green and Intelligent Technology,Chinese Academy of Sciences.
文摘Three small molecule(SM)donors,namely B-T-CN,B-TT-CN and B-DTT-CN,with differentπconjugated bridges were synthesized in this research.Interestingly,with the conjugated fused rings of theπlinking bridge increasing,the SM HOMO levels exhibit a decline tendency with–5.27 eV for B-T-CN,–5.31 eV for B-TT-CN and–5.40 eV for B-DTT-CN.After blending the SM donors with the fullerene acceptor PC71BM,the all SM organic solar cells(OSCs)achieved high Vocs of 0.90 to 0.96 V.However,the phase separation morphology and molecule stacking are also unexpectedly changed together with the enhancement of conjugated degree ofπbridges,resulting in a lower power conversion efficiency(PCE)for the B-DTT-CN:PC71BM device.Our results demonstrate and provide a useful way to enhance OSC Voc and the morphology needs to be further optimized.
基金Supported by the Fundamental Research Funds for the Central Universities,China(Grant No.ZYGX2019Z008)the National Natural Science Foundation of China(Grant No.52072061)。
文摘FeSO_(4) has the characteristics of low cost and theoretical high energy density(799 W·h·kg^(-1) with a two-electron reaction),which can meet the demand for next-generation lithium-ion batteries.Herein,FeSO_(4) as a novel highperformance conversion-reaction type cathode is investigated.We use dopamine as a carbon coating source to increase its electronic conductivity.FeSO_(4)@C demonstrates a high reversible specific capacity(512 mA·h·g^(-1))and a superior cycling performance(482 mA·h·g^(-1) after 250 cycles).In addition,we further study its reaction mechanism.The FeSO_(4) is converted to Fe and Li2SO_(4) during lithium ion insertion and the Fe-Li_(2)SO_(4) grain boundaries further store additional lithium ions.Our findings are valuable in exploring other new conversion-type lithium ion battery cathodes.
文摘Through the analysis on 211614 batches of samples from 35 categories of food safety supervision and inspection in Sichuan Province by Administration for Market Regulation of Sichuan Province in 2019,four suggestions were finally put forward.The analysis results showed that in 2019,the overall trend of food safety in Sichuan Province was stable and improved.A total of 30 categories of unqualified samples were detected,and a total of 12135 batches of unqualified samples were detected,with an average sample unqualified rate of 5.73%.Among them,the main problems of unqualified samples were as follows:the use of food additives beyond the scope and limit,accounting for 40.17%of the total number of unqualified samples,microbial contamination in food,accounting for 23.61%of the total unqualified samples,the residue index of agricultural and veterinary drugs in food not meeting the standard,accounting for 15.61%of the total unqualified samples,the quality index in food not meeting the standard,accounting for 8.51%of the total number of unqualified samples,and heavy metals and other pollutants in food exceeding the standard,accounting for 7.10%of the total unqualified samples.
文摘There is a growing demand for time series data analysis in industry areas.Apache loTDB is a time series database designed for the Internet of Things(loT)with enhanced storage and I/O performance.With User-Defined Functions(UDF)provided,computation for time series can be executed on Apache loTDB directly.To satisfy most of the common requirements in industrial time series analysis,we create a UDF library,loTDQ,on Apache loTDB.This library integrates stream computation functions on data quality analysis,data profiling,anomaly detection,data repairing,etc.loTDQ enables users to conduct a wide range of analyses,such as monitoring,error diagnosis,equipment reliability analysis.It provides a framework for users to examine loT time series with data quality problems.Experiments show that loTDQ keeps the same level of performance compared to mainstream alternatives,and shortens I/O consumption for Apache loTDB users.
基金supported by the National Natural Science Foundation of China(21325416)
文摘Dendronized hyperbranched polymer （DHP） is a new kind of polymer, which combines the advantages of dendrimers and hy- perbranched polymers. In this work, two dendronized hyperbranched polymers, DttPG0 and DHPG1, were successfully pre- pared through the simple ＂A3＋B2＂ type Sonogashira coupling reaction. The nonlinear optical （NLO） effects of DHPG0 and DItPG1, characterized by the d33 values, were 183 and 220 pm V-1 respectively, higher than those of their analogues of den- dronized polymers and dendrimers, thanks to the special topological structure. Also, the obtained polymers displayed excellent solubility, good processability, and high thermal stability.
文摘Recently N-glycosylation was found to be required for the postembryonic development and metamorphosis of the holometabolous beetle Tribolium castaneum. However, the role of N-glycosylation in the development of hemimetabolous insects is unknown. To further elucidate the role of N-glycosylation in the development of insects, a functional characterization of the N-glycosylation-related genes (NGRGs) was performed in a model insect for hemimetabolous development, namely the brown planthopper Nilaparvata lugens. In this project, we report the effects of RNAi-mediated silencing of 15 NGRGs on the postembryonic development of N. lugens. Two major observations were made. First, interruption of the early steps of N-glycan processing led to a lethal phenotype during the transition from nymph to adult as was observed in T. castaneum. Second, we report here on an essential function for the α-1,6-fucosyl transferase in the ecdysis event of N. lugens between nymphal instars, since gene-silencing by RNAi led to failure of ecdysis and subsequent mortality of the treated insect.
基金Pengyu Chen is the recipient of a doctoral grant provided by the Chinese Scholarship Council(CSC,China)This research was also funded by the Research Foundation-Flanders(FWO-Vlaanderen,Belgium)and the Special Research Fund from the Ghent University(Belgium).
文摘In mammals,plant lectinshave been shown to possess immunomodulatory properties,acting in both the innate and adaptive immune system to modulate the production of mediators of the immune response,ultimately improving host defences.At present,knowledge of immunomodulatory effects of plant lectins in insects is scarce.Treatment of insect cells with the Orysa sativa lectin,Orysata,was previously reported to induce cell aggregation,mimicking the immune process of encapsulation.In this project we investigated the potential immunomodulatory effects of this mannose-binding lectin using Drosophila melanogaster S2 cells.Identification of the Orysata binding partners on the surface of S2 cells through a pull-down assay andproteomic analysis revealed 221 putative interactors,several of which were immunity-related proteins.Subsequent qPCR analysis revealed the upregulation of Toll-and immune deficiency(IMD)-regulated antimicrobial peptides(Drs,Mtk,AttA,and Dpt)and signal transducers(Rel and Hid)belonging to the IMD pathway.In addition,the iron-binding protein Transferrin 3 was identified as a putative interactor for Orysata,and treatment of S2 cells with Orysata was shown to reduce the intracellular iron concentration.All together,we believe these results offer a new perspective on the effects by which plant lectins influence insect cells and contribute to the study of their immunomodulatory properties.
