Objective:To observe the expression of Long non-coding RNA antisense mitochondrial non-coding RNA-2 (ASncmtRNA-2) in high glucose (HG) treated human renal mesangial cells (HRMCs) and the role of ASncmtRNA-2 in oxidati...Objective:To observe the expression of Long non-coding RNA antisense mitochondrial non-coding RNA-2 (ASncmtRNA-2) in high glucose (HG) treated human renal mesangial cells (HRMCs) and the role of ASncmtRNA-2 in oxidative stress mediated diabetic nephropathy (DN) fibrosis.Methods: The expression levels of ASncmtRNA-2、transforming growth factorβ1 (TGF-β1) and fibronectin (FN) mRNA in cultured HRMCs were measured by qRT-PCR. In addition, relative reactive oxygen species (ROS) levels in HRMCs were detected with the non-fluorescent probe DCFH-DA assays.Results: Compared with 0h, the expression of ASncmtRNA-2 remained unchanged in all groups at 8 h post treatment. However, the level of ASncmtRNA-2 mRNA was increased significantly in HG and HG+NG-nitro-L-Arginine methylester (L-NAME) treated cells compared with low glucose (LG) treated cells from 16h onwards, while the level of ASncmtRNA-2 mRNA in the HG+L-NAME group was decreased compared with the HG group. Moreover, ROS fluorescence was significantly up-regulated in HG-treated cells compared with LG-treated cells, while the ROS fluorescence in HG+L-NAME group was suppressed compared with HG-treated cells. In addition, Levels of ASncmtRNA-2, TGF-β1 and FN mRNA were significantly up-regulated in HG treated cells compared with LG treated cells while Levels of ASncmtRNA-2, TGF-β1 and FN mRNA in HG+L-NAME group were down-regulated compared with HG group. Finally, the expression of ASncmtRNA-2, TGF-β1 and FN mRNA were significantly decreased in HG+ASncmtRNA-2 siRNA group compared with HG group.Conclusion: ASncmtRNA-2 was up-regulated in HG treated cells and may promote glomerular fibrosis in DN via positively regulating the expression of pro-fibrotic factors. These findings may provide novel potential therapeutic treatments for DN.展开更多
Identification and characterization of disinfection by-product(DBP)precursors could help optimize drinkingwater treatment processes and improve the quality of finishedwater.This study comprehensively investigated the ...Identification and characterization of disinfection by-product(DBP)precursors could help optimize drinkingwater treatment processes and improve the quality of finishedwater.This study comprehensively investigated the characteristics of dissolved organic matter(DOM),the hydrophilicity and molecule weight(MW)of DBP precursor and DBP-associated toxicity along the typical full-scale treatment processes.The results showed that dissolved organic carbon and dissolved organic nitrogen content,the fluorescence intensity and the SUVA254 value in raw water significantly decreased after the whole treatment processes.Conventional treatment processes were in favor of the removal of high-MW and hydrophobic DOM,which are important precursors of trihalomethane and haloacetic acid.Compared with conventional treatment processes,Ozone integrated with biological activated carbon(O3-BAC)processes enhanced the removal efficiencies of DOM with different MW and hydrophobic fractions,leading to a further decrease in almost all DBP formation potential and DBP-associated toxicity.However,almost 50%of the detected DBP precursors in raw water has not been removed after the coagulation-sedimentation-filtration integrated with O3-BAC advanced treatment processes.These remaining precursors were found to be mainly hydrophilic and low-MW(<1.0 kDa)organics.Moreover,they would largely contribute to the formation of haloacetaldehydes and haloacetonitriles,which dominated the calculated cytotoxicity.Since current drinking water treatment process could not effectively control the highly toxic DBPs,the removal of hydrophilic and low-MW organics in drinking water treatment plants should be focused on in the future.展开更多
文摘Objective:To observe the expression of Long non-coding RNA antisense mitochondrial non-coding RNA-2 (ASncmtRNA-2) in high glucose (HG) treated human renal mesangial cells (HRMCs) and the role of ASncmtRNA-2 in oxidative stress mediated diabetic nephropathy (DN) fibrosis.Methods: The expression levels of ASncmtRNA-2、transforming growth factorβ1 (TGF-β1) and fibronectin (FN) mRNA in cultured HRMCs were measured by qRT-PCR. In addition, relative reactive oxygen species (ROS) levels in HRMCs were detected with the non-fluorescent probe DCFH-DA assays.Results: Compared with 0h, the expression of ASncmtRNA-2 remained unchanged in all groups at 8 h post treatment. However, the level of ASncmtRNA-2 mRNA was increased significantly in HG and HG+NG-nitro-L-Arginine methylester (L-NAME) treated cells compared with low glucose (LG) treated cells from 16h onwards, while the level of ASncmtRNA-2 mRNA in the HG+L-NAME group was decreased compared with the HG group. Moreover, ROS fluorescence was significantly up-regulated in HG-treated cells compared with LG-treated cells, while the ROS fluorescence in HG+L-NAME group was suppressed compared with HG-treated cells. In addition, Levels of ASncmtRNA-2, TGF-β1 and FN mRNA were significantly up-regulated in HG treated cells compared with LG treated cells while Levels of ASncmtRNA-2, TGF-β1 and FN mRNA in HG+L-NAME group were down-regulated compared with HG group. Finally, the expression of ASncmtRNA-2, TGF-β1 and FN mRNA were significantly decreased in HG+ASncmtRNA-2 siRNA group compared with HG group.Conclusion: ASncmtRNA-2 was up-regulated in HG treated cells and may promote glomerular fibrosis in DN via positively regulating the expression of pro-fibrotic factors. These findings may provide novel potential therapeutic treatments for DN.
基金This work was supported by the National Key Research and Development Program of China(No.2021YFC3200700)the National Natural Science Foundation of China(No.52170009)+2 种基金the International Cooperation Project of Shanghai Science and Technology Commission(No.20230714100)the Shanghai Soft Science Project(No.20692113900)the Fundamental Research Funds for the Central Universities.
文摘Identification and characterization of disinfection by-product(DBP)precursors could help optimize drinkingwater treatment processes and improve the quality of finishedwater.This study comprehensively investigated the characteristics of dissolved organic matter(DOM),the hydrophilicity and molecule weight(MW)of DBP precursor and DBP-associated toxicity along the typical full-scale treatment processes.The results showed that dissolved organic carbon and dissolved organic nitrogen content,the fluorescence intensity and the SUVA254 value in raw water significantly decreased after the whole treatment processes.Conventional treatment processes were in favor of the removal of high-MW and hydrophobic DOM,which are important precursors of trihalomethane and haloacetic acid.Compared with conventional treatment processes,Ozone integrated with biological activated carbon(O3-BAC)processes enhanced the removal efficiencies of DOM with different MW and hydrophobic fractions,leading to a further decrease in almost all DBP formation potential and DBP-associated toxicity.However,almost 50%of the detected DBP precursors in raw water has not been removed after the coagulation-sedimentation-filtration integrated with O3-BAC advanced treatment processes.These remaining precursors were found to be mainly hydrophilic and low-MW(<1.0 kDa)organics.Moreover,they would largely contribute to the formation of haloacetaldehydes and haloacetonitriles,which dominated the calculated cytotoxicity.Since current drinking water treatment process could not effectively control the highly toxic DBPs,the removal of hydrophilic and low-MW organics in drinking water treatment plants should be focused on in the future.
