In the present study, electrical stimulation to the rat insular cortex induced apnea or respiratory disturbance, reduced amplitude of genioglossal electromyogram, and decreased electromyogram integrals. In addition, a...In the present study, electrical stimulation to the rat insular cortex induced apnea or respiratory disturbance, reduced amplitude of genioglossal electromyogram, and decreased electromyogram integrals. In addition, arterial blood gas analysis showed arterial blood acidosis, reduced pH values, increased alkali reserve negative values, decreased peripheral blood 5-hydroxytryptamine content, and increased 5-hydroxytryptamine expression in cerebellar Purkinje cells. Following lidocaine injection to block the habenular nucleus, abnormalities in breath, genioglossal electromyogram, and blood gas values disappeared, and peripheral blood 5-hydroxytryptamine content returned to levels prior to electric stimulation. However, 5-hydroxytryptamine expression in cerebellar Purkinje cells remained high. The results suggested that 5-hydroxytryptamine expression in Purkinje cells did not correlate with ventilation function involving insular cortex and habenular nucleus.展开更多
The Trachinotus ovatus is a popular aquaculture species in China.There are no obvious morphological differences between male and female fish,even during maturity,prompting research studies on sex-related features of t...The Trachinotus ovatus is a popular aquaculture species in China.There are no obvious morphological differences between male and female fish,even during maturity,prompting research studies on sex-related features of this fish.To examine sex determination-and gonadal development-related genes,we conducted transcriptome analysis of the ovaries and testes of T.ovatus.A total of 345,972,132 high-quality clean reads were obtained from 12 libraries.In addition,28,137 gonad-expressed unigenes were obtained by mapping the clean reads to the T.ovatus reference genome.A total of 8,237 differentially expressed genes(DEGs)were identified between stage I ovaries and testes,including 3,235 testicular upregulated and 5,002 ovarian upregulated genes.Furthermore,13,448 DEGs were obtained between stage III ovarian and testicular libraries,including 7,576 testicular upregulated and 5,872 ovarian upregulated genes.The DEGs included some sex-determining genes such as sry,dmrt1,and amh.DEGs between ovarian and testicular libraries were significantly enriched with KEGG pathways that are involved in gonadal development,sex determination,and gonadal function.Then,10 DEGs(seven testicular upregulated and three ovarian upregulated unigenes)were selected for quantitative real-time PCR analysis.Four genes(zinc-binding protein A33-like,pro-opiomelanocortin-like,leucine-rich repeat and transmembrane domain-containing protein 2-like,and forkhead boxC1)showed a specific testicular expression pattern.The gonadally expressed as well as testicular and ovarian DEGs provide useful information for further research on the reproductive biology of T.ovatus.展开更多
The micro turbine(MT)is scheduled to address wind power uncertainties and thus lead to greater variations in the state of natural gas system(NGS).To meet this challenge,first,a dynamic model of a gas-electricity combi...The micro turbine(MT)is scheduled to address wind power uncertainties and thus lead to greater variations in the state of natural gas system(NGS).To meet this challenge,first,a dynamic model of a gas-electricity combined system with wind power is established,the dynamic model of the NGS is based on differential equations,so that it can easily connect with the MT model;and a droop control module is added to the Rowen's MT model,so that the improved model can adjust the output power according to the scheduling instructions.Then,a stability control method based on the variable regulation ratio gas pressure regulator is proposed,the essence of this method is to reduce the variation of natural gas storage in the gas pipeline,so as to ensure the stability of pressure.The simulation results show that the dynamic model is correct and the stability control method is effective.展开更多
基金supported by the National Natural Science Foundation of China, No. 30270502
文摘In the present study, electrical stimulation to the rat insular cortex induced apnea or respiratory disturbance, reduced amplitude of genioglossal electromyogram, and decreased electromyogram integrals. In addition, arterial blood gas analysis showed arterial blood acidosis, reduced pH values, increased alkali reserve negative values, decreased peripheral blood 5-hydroxytryptamine content, and increased 5-hydroxytryptamine expression in cerebellar Purkinje cells. Following lidocaine injection to block the habenular nucleus, abnormalities in breath, genioglossal electromyogram, and blood gas values disappeared, and peripheral blood 5-hydroxytryptamine content returned to levels prior to electric stimulation. However, 5-hydroxytryptamine expression in cerebellar Purkinje cells remained high. The results suggested that 5-hydroxytryptamine expression in Purkinje cells did not correlate with ventilation function involving insular cortex and habenular nucleus.
基金the National Natural Science Foundation of China(Grant No.31660740)the Fundamental Research Funds for nonprofit research institutes under Guangxi Zhuang Autonomous Region(Grant No.GXIF-2016-19)+1 种基金Science and Technology Major Project of Guangxi[Grant number AA18242031]Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture Opening Fund(Grant No.16-380-45-B-3).
文摘The Trachinotus ovatus is a popular aquaculture species in China.There are no obvious morphological differences between male and female fish,even during maturity,prompting research studies on sex-related features of this fish.To examine sex determination-and gonadal development-related genes,we conducted transcriptome analysis of the ovaries and testes of T.ovatus.A total of 345,972,132 high-quality clean reads were obtained from 12 libraries.In addition,28,137 gonad-expressed unigenes were obtained by mapping the clean reads to the T.ovatus reference genome.A total of 8,237 differentially expressed genes(DEGs)were identified between stage I ovaries and testes,including 3,235 testicular upregulated and 5,002 ovarian upregulated genes.Furthermore,13,448 DEGs were obtained between stage III ovarian and testicular libraries,including 7,576 testicular upregulated and 5,872 ovarian upregulated genes.The DEGs included some sex-determining genes such as sry,dmrt1,and amh.DEGs between ovarian and testicular libraries were significantly enriched with KEGG pathways that are involved in gonadal development,sex determination,and gonadal function.Then,10 DEGs(seven testicular upregulated and three ovarian upregulated unigenes)were selected for quantitative real-time PCR analysis.Four genes(zinc-binding protein A33-like,pro-opiomelanocortin-like,leucine-rich repeat and transmembrane domain-containing protein 2-like,and forkhead boxC1)showed a specific testicular expression pattern.The gonadally expressed as well as testicular and ovarian DEGs provide useful information for further research on the reproductive biology of T.ovatus.
基金supported by the National Natural Science Foundation of China(51977012).
文摘The micro turbine(MT)is scheduled to address wind power uncertainties and thus lead to greater variations in the state of natural gas system(NGS).To meet this challenge,first,a dynamic model of a gas-electricity combined system with wind power is established,the dynamic model of the NGS is based on differential equations,so that it can easily connect with the MT model;and a droop control module is added to the Rowen's MT model,so that the improved model can adjust the output power according to the scheduling instructions.Then,a stability control method based on the variable regulation ratio gas pressure regulator is proposed,the essence of this method is to reduce the variation of natural gas storage in the gas pipeline,so as to ensure the stability of pressure.The simulation results show that the dynamic model is correct and the stability control method is effective.
