Purpose: Ischemia-reperfusion (I/R) injury exacerbates myocardial cell death (including apoptosis and necrosis), leading to complications such as arrhythmias, myocardial stenosis, microvascular obstruction and heart f...Purpose: Ischemia-reperfusion (I/R) injury exacerbates myocardial cell death (including apoptosis and necrosis), leading to complications such as arrhythmias, myocardial stenosis, microvascular obstruction and heart failure, and it is particularly important to seek new strategies to mitigate reperfusion injury. In this paper, we will investigate whether atorvastatin can alleviate myocardial ischemia-reperfusion injury and verify its molecular mechanism. Methods: We successfully constructed a hypoxia-reperfusion (H/R) H9c2 cell model and transfected miR-26a-5p mimic, miR-26a-5p inhibitor and its negative control NC-mimic or NC-inhibitor into H9c2 cells using a transfection kit. The expression of miR-26a-5p and FOXO1 were detected by RT-qPCR assay, the expression of related proteins by Western blot assay, the cell viability of H9c2 cells by CCK-8 assay, the apoptosis rate of H9c2 cells by flow cytometry, the CK and LDH activity in cells by CK and LDH assay kits. The targeting relationship between miR-26a-5p and FOXO1 was verified by dual luciferase reporter gene assay. Results: MiR-26a-5p expression was decreased in H/R-induced cells and FOXO1 expression was increased in H/R-induced cells. Atorvastatin alleviated H/R injury in cardiomyocytes and was most effective at a concentration of 1 μM. Atorvastatin alleviated H/R injury in cardiomyocytes by upregulating miR-26a-5p expression, miR-26a-5p and FOXO1 were negatively regulated by targeting. Conclusion: Atorvastatin can alleviate H/R injury in cardiomyocytes by regulating miR-26a-5p/FOXO1.展开更多
本研究优化甘麦大枣复合饮料制备工艺,制备甘麦大枣复合饮料,对其抗疲劳、缓解抑郁作用进行研究。通过单因素试验及正交试验优化甘麦大枣药液提取工艺及甘麦大枣复合饮料制备工艺,通过小鼠悬尾实验、强迫游泳实验和拮抗利血平实验对甘...本研究优化甘麦大枣复合饮料制备工艺,制备甘麦大枣复合饮料,对其抗疲劳、缓解抑郁作用进行研究。通过单因素试验及正交试验优化甘麦大枣药液提取工艺及甘麦大枣复合饮料制备工艺,通过小鼠悬尾实验、强迫游泳实验和拮抗利血平实验对甘麦大枣复合饮料抗疲劳及缓解抑郁作用进行研究,单因素及正交试验结果表明,甘麦大枣药液最佳提取工艺为加水量300 m L,提取温度为60℃,提取次数为3,每次提取60 min;甘麦大枣复合饮料最佳制备工艺为将药液稀释5倍,甜味剂添加量为3%,防腐剂添加量为0.1%;动物实验结果表明甘麦大枣复合饮料可以有效缩短小鼠悬尾不动时间和累计游泳不动时间;拮抗利血平实验结果表明甘麦大枣复合饮料能够提高小鼠肛温、降低小鼠眼睑下垂率及提高小鼠出圈率。本研究优选工艺制备的甘麦大枣复合饮料口感醇香,风味天然,酸甜适中,饮料呈均匀澄清的橘黄色,稳定性良好,能够起到一定抗疲劳及缓解抑郁作用。展开更多
研究全球经济海藻领域专利研发态势,为中国经济海藻技术研发提供参考。基于德温特专利数据库,采用Derwent data analyzer、Derwent innovation等分析工具和平台对1960—2020年经济海藻领域的专利文献进行分析。系统揭示半个多世纪以来...研究全球经济海藻领域专利研发态势,为中国经济海藻技术研发提供参考。基于德温特专利数据库,采用Derwent data analyzer、Derwent innovation等分析工具和平台对1960—2020年经济海藻领域的专利文献进行分析。系统揭示半个多世纪以来经济海藻的全球专利数量变化趋势、专利技术构成、代表国家或地区、主要专利权人、热点技术分布与变迁等内容,同时以产业链为分析视角,梳理热点技术的上、中、下游区位,并通过国际间对比分析得到中国在经济海藻领域技术研发面临的机遇与挑战。中国在经济海藻领域的产业链上游(如海藻栽培)、产业链中游(如药物制剂的治疗活性和提取技术)和产业链下游(如食品烹饪、添加与保存、饲料、肥料和医疗制剂等领域)占据优势,与韩国和日本的产业竞争主要存在于产业链下游,如化妆品、个人护理品和生物杀菌剂等领域,当前缺乏对生物燃料、节能环保和环境修复的研发与关注。展开更多
BACKGROUND Tenofovir alafenamide(TAF)has a serum lipid-raising effect in patients with HIV;however,its effect on serum lipids and nonalcoholic fatty liver disease(NAFLD)risk in patients with chronic hepatitis B(CHB)is...BACKGROUND Tenofovir alafenamide(TAF)has a serum lipid-raising effect in patients with HIV;however,its effect on serum lipids and nonalcoholic fatty liver disease(NAFLD)risk in patients with chronic hepatitis B(CHB)is unclear.AIM To compare the effects of TAF and entecavir(ETV)on serum lipid levels in patients with CHB.METHODS In this retrospective cohort study,the data including the clinical features,serum lipids,and metabolic factors of patients with CHB at baseline and approximately 1 year after TAF or ETV treatment were collected and analyzed.We used propensity score-matched models to assess the effects on high-density lipoprotein,lowdensity lipoprotein,triglycerides,and total cholesterol(TCHO).RESULTS A total of 336 patients(75.60%male)were included;63.69%received TAF and 36.31%received ETV.Compared with the ETV group,the TAF group had significantly higher TCHO levels after treatment(4.67±0.90 vs 4.36±1.05,P=0.006).In a propensity score-matched model for body mass index,age,sex,smoking,drinking,presence of comorbidities such as NAFLD,cirrhosis,diabetes mellitus,and hypertension,TAF-treated patients had significantly increased TCHO levels compared to that at baseline(P=0.019).There was no difference for the ETV group.Body mass index,sex,hypertension,baseline TCHO,and creatine kinase-MB isoenzyme levels were significantly associated with elevated TCHO levels in logistic regression analysis.However,1-year TAF treatment did not increase the incidence of NAFLD.CONCLUSION A greater increase in TCHO was observed in patients with CHB receiving TAF compared to those receiving ETV.However,TAF-induced dyslipidemia did not increase the incidence of NAFLD.展开更多
Domain walls(DWs)in the charge-density-wave(CDW)Mott insulator 1T-TaS_(2)have unique localized states,which play an important role in exploring the electronic properties of the material.However,the electronic states i...Domain walls(DWs)in the charge-density-wave(CDW)Mott insulator 1T-TaS_(2)have unique localized states,which play an important role in exploring the electronic properties of the material.However,the electronic states in DWs in 1TTaS_(2)have not been clearly understood,mostly due to the complex structures,phases,and interlayer stacking orders in the DW areas.Here,we explored the electronic states of DWs in the large-area CDW phase and mosaic phase of 1T-TaS_(2)by scanning tunneling spectroscopy.Due to the different densities of DWs,the electronic states of DWs show distinct features in these phases.In the large area CDW phase,both the domain and the DWs(DW1,DW2,DW4)have zero conductance at the Fermi level;while in the mosaic phase,they can be metallic or insulating depending on their environments.In areas with a high density of DWs,some electronic states were observed both on the DWs and within the domains,indicating delocalized states over the whole region.Our work contributes to further understanding of the interplay between CDW and electron correlations in 1T-TaS_(2).展开更多
Theaflavins(TFs),as the major polyphenolic components of fermented tea,possess beneficial effects on human health.In this study,the effervescent tablets based on theaflavins were developed.The optimal formulation of T...Theaflavins(TFs),as the major polyphenolic components of fermented tea,possess beneficial effects on human health.In this study,the effervescent tablets based on theaflavins were developed.The optimal formulation of TF effervescent tablets was obtained by response surface methodology with the Box-Behnken design.Then,the physiochemical properties were evaluated,including hardness,friability,effervescent time and pH of the solution.At last,the antioxidant ability of TF effervescent tablets was studied through DPPH and ABTS radical scavenging assay.According to the results,the optimal formulation of the tablets contained TF powder 9.09%,disintegrating agent 43.80%(the weight ratio of citric acid and sodium bicarbonate was 1:1),aspartame 1.86%,PEG-6003%,and mannitol in balance.With the wet granulation method,the TF effervescent tablets displayed suitable hardness,fast disintegration time,good color,pleasant taste and high antioxidant activity.This study demonstrated that the TF effervescent tablets could be a valuable product for the supplement market and contribute to promoting practical application of TFs.展开更多
文摘Purpose: Ischemia-reperfusion (I/R) injury exacerbates myocardial cell death (including apoptosis and necrosis), leading to complications such as arrhythmias, myocardial stenosis, microvascular obstruction and heart failure, and it is particularly important to seek new strategies to mitigate reperfusion injury. In this paper, we will investigate whether atorvastatin can alleviate myocardial ischemia-reperfusion injury and verify its molecular mechanism. Methods: We successfully constructed a hypoxia-reperfusion (H/R) H9c2 cell model and transfected miR-26a-5p mimic, miR-26a-5p inhibitor and its negative control NC-mimic or NC-inhibitor into H9c2 cells using a transfection kit. The expression of miR-26a-5p and FOXO1 were detected by RT-qPCR assay, the expression of related proteins by Western blot assay, the cell viability of H9c2 cells by CCK-8 assay, the apoptosis rate of H9c2 cells by flow cytometry, the CK and LDH activity in cells by CK and LDH assay kits. The targeting relationship between miR-26a-5p and FOXO1 was verified by dual luciferase reporter gene assay. Results: MiR-26a-5p expression was decreased in H/R-induced cells and FOXO1 expression was increased in H/R-induced cells. Atorvastatin alleviated H/R injury in cardiomyocytes and was most effective at a concentration of 1 μM. Atorvastatin alleviated H/R injury in cardiomyocytes by upregulating miR-26a-5p expression, miR-26a-5p and FOXO1 were negatively regulated by targeting. Conclusion: Atorvastatin can alleviate H/R injury in cardiomyocytes by regulating miR-26a-5p/FOXO1.
文摘本研究优化甘麦大枣复合饮料制备工艺,制备甘麦大枣复合饮料,对其抗疲劳、缓解抑郁作用进行研究。通过单因素试验及正交试验优化甘麦大枣药液提取工艺及甘麦大枣复合饮料制备工艺,通过小鼠悬尾实验、强迫游泳实验和拮抗利血平实验对甘麦大枣复合饮料抗疲劳及缓解抑郁作用进行研究,单因素及正交试验结果表明,甘麦大枣药液最佳提取工艺为加水量300 m L,提取温度为60℃,提取次数为3,每次提取60 min;甘麦大枣复合饮料最佳制备工艺为将药液稀释5倍,甜味剂添加量为3%,防腐剂添加量为0.1%;动物实验结果表明甘麦大枣复合饮料可以有效缩短小鼠悬尾不动时间和累计游泳不动时间;拮抗利血平实验结果表明甘麦大枣复合饮料能够提高小鼠肛温、降低小鼠眼睑下垂率及提高小鼠出圈率。本研究优选工艺制备的甘麦大枣复合饮料口感醇香,风味天然,酸甜适中,饮料呈均匀澄清的橘黄色,稳定性良好,能够起到一定抗疲劳及缓解抑郁作用。
文摘研究全球经济海藻领域专利研发态势,为中国经济海藻技术研发提供参考。基于德温特专利数据库,采用Derwent data analyzer、Derwent innovation等分析工具和平台对1960—2020年经济海藻领域的专利文献进行分析。系统揭示半个多世纪以来经济海藻的全球专利数量变化趋势、专利技术构成、代表国家或地区、主要专利权人、热点技术分布与变迁等内容,同时以产业链为分析视角,梳理热点技术的上、中、下游区位,并通过国际间对比分析得到中国在经济海藻领域技术研发面临的机遇与挑战。中国在经济海藻领域的产业链上游(如海藻栽培)、产业链中游(如药物制剂的治疗活性和提取技术)和产业链下游(如食品烹饪、添加与保存、饲料、肥料和医疗制剂等领域)占据优势,与韩国和日本的产业竞争主要存在于产业链下游,如化妆品、个人护理品和生物杀菌剂等领域,当前缺乏对生物燃料、节能环保和环境修复的研发与关注。
基金Supported by Natural Science Foundation of Fujian Province,No.2021J01123300.
文摘BACKGROUND Tenofovir alafenamide(TAF)has a serum lipid-raising effect in patients with HIV;however,its effect on serum lipids and nonalcoholic fatty liver disease(NAFLD)risk in patients with chronic hepatitis B(CHB)is unclear.AIM To compare the effects of TAF and entecavir(ETV)on serum lipid levels in patients with CHB.METHODS In this retrospective cohort study,the data including the clinical features,serum lipids,and metabolic factors of patients with CHB at baseline and approximately 1 year after TAF or ETV treatment were collected and analyzed.We used propensity score-matched models to assess the effects on high-density lipoprotein,lowdensity lipoprotein,triglycerides,and total cholesterol(TCHO).RESULTS A total of 336 patients(75.60%male)were included;63.69%received TAF and 36.31%received ETV.Compared with the ETV group,the TAF group had significantly higher TCHO levels after treatment(4.67±0.90 vs 4.36±1.05,P=0.006).In a propensity score-matched model for body mass index,age,sex,smoking,drinking,presence of comorbidities such as NAFLD,cirrhosis,diabetes mellitus,and hypertension,TAF-treated patients had significantly increased TCHO levels compared to that at baseline(P=0.019).There was no difference for the ETV group.Body mass index,sex,hypertension,baseline TCHO,and creatine kinase-MB isoenzyme levels were significantly associated with elevated TCHO levels in logistic regression analysis.However,1-year TAF treatment did not increase the incidence of NAFLD.CONCLUSION A greater increase in TCHO was observed in patients with CHB receiving TAF compared to those receiving ETV.However,TAF-induced dyslipidemia did not increase the incidence of NAFLD.
基金Project supported by the National Key Research and Development Project of China(Grant No.2019YFA0308500)the National Natural Science Foundation of China(Grant No.61888102)the Chinese Academy of Sciences(Grant Nos.XDB30000000 and YSBR-003).
文摘Domain walls(DWs)in the charge-density-wave(CDW)Mott insulator 1T-TaS_(2)have unique localized states,which play an important role in exploring the electronic properties of the material.However,the electronic states in DWs in 1TTaS_(2)have not been clearly understood,mostly due to the complex structures,phases,and interlayer stacking orders in the DW areas.Here,we explored the electronic states of DWs in the large-area CDW phase and mosaic phase of 1T-TaS_(2)by scanning tunneling spectroscopy.Due to the different densities of DWs,the electronic states of DWs show distinct features in these phases.In the large area CDW phase,both the domain and the DWs(DW1,DW2,DW4)have zero conductance at the Fermi level;while in the mosaic phase,they can be metallic or insulating depending on their environments.In areas with a high density of DWs,some electronic states were observed both on the DWs and within the domains,indicating delocalized states over the whole region.Our work contributes to further understanding of the interplay between CDW and electron correlations in 1T-TaS_(2).
基金Supported by Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization,Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains Fund(2021CX06)。
文摘Theaflavins(TFs),as the major polyphenolic components of fermented tea,possess beneficial effects on human health.In this study,the effervescent tablets based on theaflavins were developed.The optimal formulation of TF effervescent tablets was obtained by response surface methodology with the Box-Behnken design.Then,the physiochemical properties were evaluated,including hardness,friability,effervescent time and pH of the solution.At last,the antioxidant ability of TF effervescent tablets was studied through DPPH and ABTS radical scavenging assay.According to the results,the optimal formulation of the tablets contained TF powder 9.09%,disintegrating agent 43.80%(the weight ratio of citric acid and sodium bicarbonate was 1:1),aspartame 1.86%,PEG-6003%,and mannitol in balance.With the wet granulation method,the TF effervescent tablets displayed suitable hardness,fast disintegration time,good color,pleasant taste and high antioxidant activity.This study demonstrated that the TF effervescent tablets could be a valuable product for the supplement market and contribute to promoting practical application of TFs.
