This study endeavors to formulate a comprehensive methodology for establishing a Geological Knowledge Base(GKB)tailored to fracture-cavity reservoir outcrops within the North Tarim Basin.The acquisition of quantitativ...This study endeavors to formulate a comprehensive methodology for establishing a Geological Knowledge Base(GKB)tailored to fracture-cavity reservoir outcrops within the North Tarim Basin.The acquisition of quantitative geological parameters was accomplished through diverse means such as outcrop observations,thin section studies,unmanned aerial vehicle scanning,and high-resolution cameras.Subsequently,a three-dimensional digital outcrop model was generated,and the parameters were standardized.An assessment of traditional geological knowledge was conducted to delineate the knowledge framework,content,and system of the GKB.The basic parameter knowledge was extracted using multiscale fine characterization techniques,including core statistics,field observations,and microscopic thin section analysis.Key mechanism knowledge was identified by integrating trace elements from filling,isotope geochemical tests,and water-rock simulation experiments.Significant representational knowledge was then extracted by employing various methods such as multiple linear regression,neural network technology,and discriminant classification.Subsequently,an analogy study was performed on the karst fracture-cavity system(KFCS)in both outcrop and underground reservoir settings.The results underscored several key findings:(1)Utilization of a diverse range of techniques,including outcrop observations,core statistics,unmanned aerial vehicle scanning,high-resolution cameras,thin section analysis,and electron scanning imaging,enabled the acquisition and standardization of data.This facilitated effective management and integration of geological parameter data from multiple sources and scales.(2)The GKB for fracture-cavity reservoir outcrops,encompassing basic parameter knowledge,key mechanism knowledge,and significant representational knowledge,provides robust data support and systematic geological insights for the intricate and in-depth examination of the genetic mechanisms of fracture-cavity reservoirs.(3)The developmental characteristics of fracturecavities in karst outcrops offer effective,efficient,and accurate guidance for fracture-cavity research in underground karst reservoirs.The outlined construction method of the outcrop geological knowledge base is applicable to various fracture-cavity reservoirs in different layers and regions worldwide.展开更多
Natural gas and condensate derived from Carboniferous-Permian(C-P)coaly source rocks discovered in the Dagang Oilfield in the Bohai Bay Basin(east China)have important implications for the potential exploration of C-P...Natural gas and condensate derived from Carboniferous-Permian(C-P)coaly source rocks discovered in the Dagang Oilfield in the Bohai Bay Basin(east China)have important implications for the potential exploration of C-P coaly source rocks.This study analyzed the secondary,tertiary,and dynamic characteristics of hydrocarbon generation in order to predict the hydrocarbon potentials of different exploration areas in the Dagang Oilfield.The results indicated that C-P oil and gas were generated from coaly source rocks by secondary or tertiary hydrocarbon generation and characterized by notably different hydrocarbon products and generation dynamics.Secondary hydrocarbon generation was completed when the maturity reached vitrinite reflectance(Ro)of 0.7%-0.9%before uplift prior to the Eocene.Tertiary hydrocarbon generation from the source rocks was limited in deep buried sags in the Oligocene,where the products consisted of light oil and gas.The activation energies for secondary and tertiary hydrocarbon generation were 260-280 kJ/mol and 300-330 kJ/mol,respectively,indicating that each instance of hydrocarbon generation required higher temperature or deeper burial than the previous instance.Locations with secondary or tertiary hydrocarbon generation from C-P coaly source rocks were interpreted as potential oil and gas exploration regions.展开更多
The mechanisms of lacustrine organic-rich shale formation have attracted attention due to its association with global shale oil and shale gas exploration.Samples of general-quality and excellent-quality source rocks,a...The mechanisms of lacustrine organic-rich shale formation have attracted attention due to its association with global shale oil and shale gas exploration.Samples of general-quality and excellent-quality source rocks,and oil shale from the Beibu Gulf Basin were analyzed to investigate their organic geochemistry,palynofacies,and trace elements.Hydrocarbon potential was higher in the oil shale(29.79 mg/g)than in the general-quality source rock(3.82 mg/g),and its kerogen type wasⅠ-Ⅱ2.Hydrogen-rich liptinite(cutinite and sporinite)components derived from terrigenous higher plants provided most of the hydrocarbon potential of excellent-quality source rock and oil shale.Under the influence of depressioncontrolling fault activity,a deeper subsidence center promotes the deposition of excellent-quality source rock and oil shale in brackish-hypoxic bottom water.A shallower subsidence center,due to subsag-controlling fault activity,promotes the formation of excellent-quality source rock under freshbrackish and weak oxidation-weak reducing conditions.The local uplift and shallow-slope led to the formation of general-quality source rock,under freshwater weak-oxidation conditions.A model was established for organic matter(OM)accumulation in organic-rich shales,accounting for fault activity,terrigenous hydrogen-rich OM,and the preservation conditions,to predict the development of excellent-quality source rock from areas with low levels of exploration.展开更多
The human UDP-glucuronosyltransferase 1A1(UGT1A1),one of the most essential conjugative enzymes,is responsible for the metabolism and detoxification of bilirubin and other endogenous substances,as well as many differe...The human UDP-glucuronosyltransferase 1A1(UGT1A1),one of the most essential conjugative enzymes,is responsible for the metabolism and detoxification of bilirubin and other endogenous substances,as well as many different xenobiotic compounds.Deciphering UGT1A1 relevance to human diseases and characterizing the effects of small molecules on the activities of UGT1A1 requires reliable tools for probing the function of this key enzyme in complex biological matrices.Herein,an easy-to-use assay for highly-selective and sensitive monitoring of UGT1A1 activities in various biological matrices,using liquid chromatography with fluorescence detection(LC-FD),has been developed and validated.The newly developed LC-FD based assay has been confirmed in terms of sensitivity,specificity,precision,quantitative linear range and stability.One of its main advantages is lowering the limits of detection and quantification by about 100-fold in comparison to the previous assay that used the same probe substrate,enabling reliable quantification of lower amounts of active enzyme than any other method.The precision test demonstrated that both intra-and inter-day variations for this assay were less than 5.5%.Furthermore,the newly developed assay has also been successfully used to screen and characterize the regulatory effects of small molecules on the expression level of UGT1A1 in living cells.Ove rall,an easy-to-use LC-FD based assay has been developed for ultra-sensitive UGT1A1 activities measurements in various biological systems,providing an inexpensive and practical approach for exploring the role of UGT1A1 in human diseases,interactions with xenobiotics,and characterization modulatory effects of small molecules on this conjugative enzyme.展开更多
Walnut(Juglans regia L.)is a good source of lipids and polyunsaturated fatty acids(PUFAs).In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid(FA)synthesis in walnut,the samples ...Walnut(Juglans regia L.)is a good source of lipids and polyunsaturated fatty acids(PUFAs).In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid(FA)synthesis in walnut,the samples at different development periods of three walnut cultivars,’Zhipi’(ZP),’Xinwu 417’(W417)and’Xinwen 81’(W81)were collected for transcriptomic analysis.The analysis of oil accumulation and FA profiles showed that the oil content in mature walnut kernel was nearly 70%,and over 90%of FAs were PUFAs.We identified 126 candidate genes including 64 genes for FA de novo synthesis,45 genes for triacylglycerol assembly,and 17 genes for oil bodies involved in lipid biosynthesis by RNA-sequencing.Ten key enzymes including ACCase,LACS6,LACS8,SAD,FAD2,FAD3,LPAAT1,DGAT2,PDAT2,and PLC encoded by 19 genes were highly associated with lipid biosynthesis.Quantitative PCR analysis further validated 9 important genes,and the results were well consistent with our transcriptomic data.Finally,5 important transcription factors including WRI1,ABI3,FUS3,PKL and VAL1 were identified,and their main regulatory genes might contain ACCase,KASII,LACS,FAD3 and LPAAT which were determined through correlation analysis of expression levels for 27 walnut samples.These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in walnut.展开更多
Lithium-selenium(Li-Se) battery is a promising system with high theoretical gravimetric and volumetric energy densities, while its long-term cyclability is hard to realize, especially when a practical Se cathode with ...Lithium-selenium(Li-Se) battery is a promising system with high theoretical gravimetric and volumetric energy densities, while its long-term cyclability is hard to realize, especially when a practical Se cathode with high Se content, high Se loading, and high density is employed. The main obstacles are the sluggish conversion kinetics of the dense Se cathodes and the continuous deterioration of the Li-metal anodes.Here, by introducing an acetonitrile(AN)-based electrolyte and replacing the Li electrode with a lithiated graphite, we successfully build a hybrid conversion-intercalation system using a high-content(80 wt%),decent-loading(3.0 mg cm^(-2)), and low-porosity(44%) Se cathode. The as-designed lithiated graphite||Se(LG||Se) cell demonstrated a high Se utilization(97.4%), a long cycle life(3000 cycles), and an ultrahigh average Coulombic efficiency(99.98%). The cell also works well under lean-electrolyte(2 l L mg^(-1)) condition and shows outstanding safety performance in the nail-penetrating test. The combination affords the competitive comprehensive performances, including high volumetric and gravimetric energy densities, long cycling life, and superb safety of the LG||Se cell. In addition, with a newly-designed threeelectrode pouch cell, the lithiation of the graphite anodes could be done with an in-situ lithiation process,indicating the high potential of the as-proposed LG||Se cell for the practical applications.展开更多
基金supported by the Major Scientific and Technological Projects of CNPC under grant ZD2019-183-006the National Science and Technology Major Project of China (2016ZX05014002-006)the National Natural Science Foundation of China (42072234,42272180)。
文摘This study endeavors to formulate a comprehensive methodology for establishing a Geological Knowledge Base(GKB)tailored to fracture-cavity reservoir outcrops within the North Tarim Basin.The acquisition of quantitative geological parameters was accomplished through diverse means such as outcrop observations,thin section studies,unmanned aerial vehicle scanning,and high-resolution cameras.Subsequently,a three-dimensional digital outcrop model was generated,and the parameters were standardized.An assessment of traditional geological knowledge was conducted to delineate the knowledge framework,content,and system of the GKB.The basic parameter knowledge was extracted using multiscale fine characterization techniques,including core statistics,field observations,and microscopic thin section analysis.Key mechanism knowledge was identified by integrating trace elements from filling,isotope geochemical tests,and water-rock simulation experiments.Significant representational knowledge was then extracted by employing various methods such as multiple linear regression,neural network technology,and discriminant classification.Subsequently,an analogy study was performed on the karst fracture-cavity system(KFCS)in both outcrop and underground reservoir settings.The results underscored several key findings:(1)Utilization of a diverse range of techniques,including outcrop observations,core statistics,unmanned aerial vehicle scanning,high-resolution cameras,thin section analysis,and electron scanning imaging,enabled the acquisition and standardization of data.This facilitated effective management and integration of geological parameter data from multiple sources and scales.(2)The GKB for fracture-cavity reservoir outcrops,encompassing basic parameter knowledge,key mechanism knowledge,and significant representational knowledge,provides robust data support and systematic geological insights for the intricate and in-depth examination of the genetic mechanisms of fracture-cavity reservoirs.(3)The developmental characteristics of fracturecavities in karst outcrops offer effective,efficient,and accurate guidance for fracture-cavity research in underground karst reservoirs.The outlined construction method of the outcrop geological knowledge base is applicable to various fracture-cavity reservoirs in different layers and regions worldwide.
基金supported by the Certificate of National Science and Technology Major Project of the Ministry of Science and Technology of China(2016ZX05006007-004,2016ZX05014)National Natural Science Foundation of China(41702139,U1663204,41602138,41602142)+1 种基金Natural Science Foundation of Shandong Province(ZR2017BD036,ZR2017LD005)Fundamental Research Funds for the Central Universities(18CX02008A)。
文摘Natural gas and condensate derived from Carboniferous-Permian(C-P)coaly source rocks discovered in the Dagang Oilfield in the Bohai Bay Basin(east China)have important implications for the potential exploration of C-P coaly source rocks.This study analyzed the secondary,tertiary,and dynamic characteristics of hydrocarbon generation in order to predict the hydrocarbon potentials of different exploration areas in the Dagang Oilfield.The results indicated that C-P oil and gas were generated from coaly source rocks by secondary or tertiary hydrocarbon generation and characterized by notably different hydrocarbon products and generation dynamics.Secondary hydrocarbon generation was completed when the maturity reached vitrinite reflectance(Ro)of 0.7%-0.9%before uplift prior to the Eocene.Tertiary hydrocarbon generation from the source rocks was limited in deep buried sags in the Oligocene,where the products consisted of light oil and gas.The activation energies for secondary and tertiary hydrocarbon generation were 260-280 kJ/mol and 300-330 kJ/mol,respectively,indicating that each instance of hydrocarbon generation required higher temperature or deeper burial than the previous instance.Locations with secondary or tertiary hydrocarbon generation from C-P coaly source rocks were interpreted as potential oil and gas exploration regions.
基金supported by the Certificate of National Science and Technology Major Project of the Ministry of Science and Technology of China(2016ZX05006-007-004)National Natural Science Foundation of China(41702139,41772138,41602142,42072130)the Fundamental Research Funds for the Central Universities(18CX02008A)。
文摘The mechanisms of lacustrine organic-rich shale formation have attracted attention due to its association with global shale oil and shale gas exploration.Samples of general-quality and excellent-quality source rocks,and oil shale from the Beibu Gulf Basin were analyzed to investigate their organic geochemistry,palynofacies,and trace elements.Hydrocarbon potential was higher in the oil shale(29.79 mg/g)than in the general-quality source rock(3.82 mg/g),and its kerogen type wasⅠ-Ⅱ2.Hydrogen-rich liptinite(cutinite and sporinite)components derived from terrigenous higher plants provided most of the hydrocarbon potential of excellent-quality source rock and oil shale.Under the influence of depressioncontrolling fault activity,a deeper subsidence center promotes the deposition of excellent-quality source rock and oil shale in brackish-hypoxic bottom water.A shallower subsidence center,due to subsag-controlling fault activity,promotes the formation of excellent-quality source rock under freshbrackish and weak oxidation-weak reducing conditions.The local uplift and shallow-slope led to the formation of general-quality source rock,under freshwater weak-oxidation conditions.A model was established for organic matter(OM)accumulation in organic-rich shales,accounting for fault activity,terrigenous hydrogen-rich OM,and the preservation conditions,to predict the development of excellent-quality source rock from areas with low levels of exploration.
基金finically supported by the NSF of China(81773687,81922070,81973286,81703604)the National Key Research and Development Program of China(2017YFC1700200,2017YFC1702000)+5 种基金the Open Project Program of Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica(No.JKLPSE-201803)the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)Program of Shanghai Academic/Technology Research Leader(18XD1403600)Drug Innovation Major Project(2018ZX09731016)Shuguang Program(18SG40)supported by Shanghai Education Development Foundation and Shanghai Municipal Education Commissionthe Graduate Innovation Project of Shanghai University of Traditional Chinese Medicine(Y2019063)。
文摘The human UDP-glucuronosyltransferase 1A1(UGT1A1),one of the most essential conjugative enzymes,is responsible for the metabolism and detoxification of bilirubin and other endogenous substances,as well as many different xenobiotic compounds.Deciphering UGT1A1 relevance to human diseases and characterizing the effects of small molecules on the activities of UGT1A1 requires reliable tools for probing the function of this key enzyme in complex biological matrices.Herein,an easy-to-use assay for highly-selective and sensitive monitoring of UGT1A1 activities in various biological matrices,using liquid chromatography with fluorescence detection(LC-FD),has been developed and validated.The newly developed LC-FD based assay has been confirmed in terms of sensitivity,specificity,precision,quantitative linear range and stability.One of its main advantages is lowering the limits of detection and quantification by about 100-fold in comparison to the previous assay that used the same probe substrate,enabling reliable quantification of lower amounts of active enzyme than any other method.The precision test demonstrated that both intra-and inter-day variations for this assay were less than 5.5%.Furthermore,the newly developed assay has also been successfully used to screen and characterize the regulatory effects of small molecules on the expression level of UGT1A1 in living cells.Ove rall,an easy-to-use LC-FD based assay has been developed for ultra-sensitive UGT1A1 activities measurements in various biological systems,providing an inexpensive and practical approach for exploring the role of UGT1A1 in human diseases,interactions with xenobiotics,and characterization modulatory effects of small molecules on this conjugative enzyme.
基金supported by Major scientific and technological projects of Xinjiang Production and Construction Corps(2017DB006 and 2020KWZ-012)。
文摘Walnut(Juglans regia L.)is a good source of lipids and polyunsaturated fatty acids(PUFAs).In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid(FA)synthesis in walnut,the samples at different development periods of three walnut cultivars,’Zhipi’(ZP),’Xinwu 417’(W417)and’Xinwen 81’(W81)were collected for transcriptomic analysis.The analysis of oil accumulation and FA profiles showed that the oil content in mature walnut kernel was nearly 70%,and over 90%of FAs were PUFAs.We identified 126 candidate genes including 64 genes for FA de novo synthesis,45 genes for triacylglycerol assembly,and 17 genes for oil bodies involved in lipid biosynthesis by RNA-sequencing.Ten key enzymes including ACCase,LACS6,LACS8,SAD,FAD2,FAD3,LPAAT1,DGAT2,PDAT2,and PLC encoded by 19 genes were highly associated with lipid biosynthesis.Quantitative PCR analysis further validated 9 important genes,and the results were well consistent with our transcriptomic data.Finally,5 important transcription factors including WRI1,ABI3,FUS3,PKL and VAL1 were identified,and their main regulatory genes might contain ACCase,KASII,LACS,FAD3 and LPAAT which were determined through correlation analysis of expression levels for 27 walnut samples.These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in walnut.
基金supported by the National Natural Science Foundation of China under Grant No. 51802225the funding from the State Key Laboratory of Materials Processing and Die & Mould Technology, Huazhong University of Science and Technology (P2020-001)。
文摘Lithium-selenium(Li-Se) battery is a promising system with high theoretical gravimetric and volumetric energy densities, while its long-term cyclability is hard to realize, especially when a practical Se cathode with high Se content, high Se loading, and high density is employed. The main obstacles are the sluggish conversion kinetics of the dense Se cathodes and the continuous deterioration of the Li-metal anodes.Here, by introducing an acetonitrile(AN)-based electrolyte and replacing the Li electrode with a lithiated graphite, we successfully build a hybrid conversion-intercalation system using a high-content(80 wt%),decent-loading(3.0 mg cm^(-2)), and low-porosity(44%) Se cathode. The as-designed lithiated graphite||Se(LG||Se) cell demonstrated a high Se utilization(97.4%), a long cycle life(3000 cycles), and an ultrahigh average Coulombic efficiency(99.98%). The cell also works well under lean-electrolyte(2 l L mg^(-1)) condition and shows outstanding safety performance in the nail-penetrating test. The combination affords the competitive comprehensive performances, including high volumetric and gravimetric energy densities, long cycling life, and superb safety of the LG||Se cell. In addition, with a newly-designed threeelectrode pouch cell, the lithiation of the graphite anodes could be done with an in-situ lithiation process,indicating the high potential of the as-proposed LG||Se cell for the practical applications.
