Objective:SOX11 is expressed in numerous malignancies,including hepatocellular carcinomas(HCC),but its oncogenic function has not been elucidated.Here,we performed a comprehensive bioinformatics analysis of the Liver ...Objective:SOX11 is expressed in numerous malignancies,including hepatocellular carcinomas(HCC),but its oncogenic function has not been elucidated.Here,we performed a comprehensive bioinformatics analysis of the Liver Hepatocellular Carcinoma(LIHC)dataset to investigate the function of SOX11 in tumorgenesis.Methods:SOX11 expression data from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)were validated by immunohistochemistry(IHC).Co-expression,differential expression,and functional analyses utilized TCGA-LIHC,Timer 2.0,Metascape,GTEx,and LinkedOmics databases.Associations with immune infiltration,ferroptosis,and immune checkpoint genes were assessed.Genetic changes were explored via CBioPortal.Logistic regression,receiver operating characteristic curve(ROC),Kaplan-Meier analysis,and nomogram modeling evaluated associations with HCC clinicopathological features.SOX11’s impact on proliferation and migration was studied in HepG2 and HuH7 cell lines.Results:SOX11 was significantly elevated in HCC tumors compared to controls.SOX11-associated genes exhibited differential expression in pathways involving extracellular membrane ion channels.Significant associations were found between SOX11 levels,immune infiltration,ferroptosis,and immune checkpoint genes in HCC tissue.SOX11 levels correlated with HCC stage,histologic grade,and tumor status,and independently predicted overall and disease-specific survival.SOX11 expression effectively distinguished between tumor and normal liver tissue.Spearman correlations highlighted a significant relationship between SOX11 and ferroptosis-associated genes.Decreased SOX11 levels in HepG2 and HuH7 cells resulted in reduced proliferation and migration.Conclusions:SOX11 was found to represent a promising biomarker within HCC diagnosis and prognosis together with being a possible drug-target.展开更多
Urinary kidney injury molecule 1(uKIM-1)serves as a reliable marker for the early diagnosis of acute kidney injury(AKI).The rapid and facile detection of changes in uKIM-1 is essential for early AKI diagnosis,ultimate...Urinary kidney injury molecule 1(uKIM-1)serves as a reliable marker for the early diagnosis of acute kidney injury(AKI).The rapid and facile detection of changes in uKIM-1 is essential for early AKI diagnosis,ultimately improving the prognosis of patients.In this study,we developed a fully printed photonic crystal-integrated microarray with photonic crystal-enhanced fluorescence properties,which can detect uKIM-1 levels at the point-of-care.We confirmed its efficacy in the early diagnosis of AKI using clinical urine specimens.Direct quantitative detection of uKIM-1 was achieved within 10 min.The lowest limit of detection is 8.75 pg·mL^(-1) with an accuracy of 94.2%.The diagnostic efficacy was validated using 86 clinical urine samples,highlighting the high sensitivity and stability of the photonic crystal microarray.Consequently,a facile and reliable immunoassay was designed and prepared for the rapid quantitative detection of uKIM-1,which is crucial for the early identification and convenient detection of AKI in hospital or community settings.Rapid,convenient,cost-effective,and long-term monitoring of changes in uKIM-1 levels can assist clinicians in making timely adjustments to treatment regimens,preventing the transition from AKI to chronic kidney disease(CKD),improving the quality of life of patients with AKI,and reducing healthcare costs.It highlights the advantages of utilizing urine samples as a noninvasive and easily accessible medium for early detection and monitoring of kidney-related conditions.展开更多
To the Editor:Globally,acute kidney injury(AKI)is a common life-threatening condition.AKI is more prevalent in elderly patients,with increasing mortality rates.Compared with younger patients,older patients with AKI ha...To the Editor:Globally,acute kidney injury(AKI)is a common life-threatening condition.AKI is more prevalent in elderly patients,with increasing mortality rates.Compared with younger patients,older patients with AKI have a higher risk of persistent injury and poorer prognoses.[1]Older individuals often have comorbidities,increasing their susceptibility to AKI.Kidney Disease:Improving Global Outcomes(KDIGO)criteria define and stage AKI based on the higher stage of AKI defined by either serum creatinine(SCr)level or urine output(UO).[2]However,the record of UO is difficult to access in clinic,the data on the value of UO combined with SCr for AKI diagnosis or prognosis in elderly patients are lacking.Therefore,the objectives of this study were to investigate whether UO would influence the classification of AKI and improve the accuracy of prediction of the clinical prognosis in elderly patients.展开更多
Background:Cardiorenal syndrome is increasingly common and has been reported to be associated with inflammation and oxidative stress,and statins have anti-inflammatory and antioxidant effects.Therefore,we designed th...Background:Cardiorenal syndrome is increasingly common and has been reported to be associated with inflammation and oxidative stress,and statins have anti-inflammatory and antioxidant effects.Therefore,we designed this experiment to study the preventive effect of statins on cardiorenal syndrome.The aim of the study is to investigate the effect of early rosuvastatin use on cardiorenal syndrome.Method:Forty-five Wistar rats were randomly divided into 3 groups.A unilateral nephrectomy group(Group 1),a unilateral nephrectomy+coronary ligation group(Group 2),and a unilateral nephrectomy+coronary ligation+rosuvastatin group(Group 3).Right kidney removal was performed on all rats during the first week,while Group 3 was given statin intragastric administration at 10 mg/kg/d.One month later,coronary ligation was performed on rats in Groups 2 and 3.Group 3 continued statin treatment.After feeding for 3 months and 2 days,the rats were killed;urine and blood were collected and sent to the laboratory for the determination of the urinary protein/creatinine ratio and blood lipid,creatinine,and urea nitrogen levels,respectively.Serum interleukin 1β,interleukin 6,malondialdehyde,glutathione peroxidase,angiotensin II,neutrophil gelatinase-associated lipocalin,cystatin C,and B natriuretic peptide levels were also determined.On the day before euthanasia,all rats were anesthetized and examined by cardiac ultrasound.Hematoxylin-eosin and periodic acid–Schiff staining were performed on heart and kidney sections.Results:The ejection fraction in Group 2 was lower than that in Group 1(P<0.01).The ejection fraction value in Group 3 was lower than that in Group 1(P<0.01).Interleukin-1βlevels in Group 2 were higher than those in Group 1(P<0.01).Interleukin-1βlevels in Group 3 were lower than those in Group 2(P<0.01).The malondialdehyde value in Group 3 was lower than that in Group 2(P<0.05).Histopathology showed that Group 1 had slight renal damage,renal injury was aggravated in Group 2,and renal injury was still present in Group 3,but with alleviated morphology.Conclusion:The interaction of the heart and kidneys in rats is related to inflammation and oxidation.Rosuvastatin can slow down the development of the heart-kidney interaction through anti-inflammatory and antioxidant effects.展开更多
基金supported by grants from Guizhou Nursing Vocational College Foundation(No.gzhlyj2023-04)Guizhou Nursing Vocational College Foundation(No.gzhlyj2021-02)+1 种基金Science and Technology Foundation of Guizhou Provincial Health Committee(No.gzwkj2022-518)Nature Science Foundation of Beijing,China(No.7214253).
文摘Objective:SOX11 is expressed in numerous malignancies,including hepatocellular carcinomas(HCC),but its oncogenic function has not been elucidated.Here,we performed a comprehensive bioinformatics analysis of the Liver Hepatocellular Carcinoma(LIHC)dataset to investigate the function of SOX11 in tumorgenesis.Methods:SOX11 expression data from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)were validated by immunohistochemistry(IHC).Co-expression,differential expression,and functional analyses utilized TCGA-LIHC,Timer 2.0,Metascape,GTEx,and LinkedOmics databases.Associations with immune infiltration,ferroptosis,and immune checkpoint genes were assessed.Genetic changes were explored via CBioPortal.Logistic regression,receiver operating characteristic curve(ROC),Kaplan-Meier analysis,and nomogram modeling evaluated associations with HCC clinicopathological features.SOX11’s impact on proliferation and migration was studied in HepG2 and HuH7 cell lines.Results:SOX11 was significantly elevated in HCC tumors compared to controls.SOX11-associated genes exhibited differential expression in pathways involving extracellular membrane ion channels.Significant associations were found between SOX11 levels,immune infiltration,ferroptosis,and immune checkpoint genes in HCC tissue.SOX11 levels correlated with HCC stage,histologic grade,and tumor status,and independently predicted overall and disease-specific survival.SOX11 expression effectively distinguished between tumor and normal liver tissue.Spearman correlations highlighted a significant relationship between SOX11 and ferroptosis-associated genes.Decreased SOX11 levels in HepG2 and HuH7 cells resulted in reduced proliferation and migration.Conclusions:SOX11 was found to represent a promising biomarker within HCC diagnosis and prognosis together with being a possible drug-target.
基金supported by the National Natural Science Foundation of China(Nos.82170684,52222313,22075296,91963212,82000004)the Health Care Program Foundation of PLA(No.21BJZ17)+2 种基金the Youth Independent Innovation Science Fund of the General Hospital of the People’s Liberation Army(No.22QNFC007)the Youth Innovation Promotion Association CAS(No.2020032)the Intramural Research Fund of Peking University International Hospital(No.YN2021QN05).
文摘Urinary kidney injury molecule 1(uKIM-1)serves as a reliable marker for the early diagnosis of acute kidney injury(AKI).The rapid and facile detection of changes in uKIM-1 is essential for early AKI diagnosis,ultimately improving the prognosis of patients.In this study,we developed a fully printed photonic crystal-integrated microarray with photonic crystal-enhanced fluorescence properties,which can detect uKIM-1 levels at the point-of-care.We confirmed its efficacy in the early diagnosis of AKI using clinical urine specimens.Direct quantitative detection of uKIM-1 was achieved within 10 min.The lowest limit of detection is 8.75 pg·mL^(-1) with an accuracy of 94.2%.The diagnostic efficacy was validated using 86 clinical urine samples,highlighting the high sensitivity and stability of the photonic crystal microarray.Consequently,a facile and reliable immunoassay was designed and prepared for the rapid quantitative detection of uKIM-1,which is crucial for the early identification and convenient detection of AKI in hospital or community settings.Rapid,convenient,cost-effective,and long-term monitoring of changes in uKIM-1 levels can assist clinicians in making timely adjustments to treatment regimens,preventing the transition from AKI to chronic kidney disease(CKD),improving the quality of life of patients with AKI,and reducing healthcare costs.It highlights the advantages of utilizing urine samples as a noninvasive and easily accessible medium for early detection and monitoring of kidney-related conditions.
基金Special Scientific Research Project of Military Health Care(No.21BJZ17)
文摘To the Editor:Globally,acute kidney injury(AKI)is a common life-threatening condition.AKI is more prevalent in elderly patients,with increasing mortality rates.Compared with younger patients,older patients with AKI have a higher risk of persistent injury and poorer prognoses.[1]Older individuals often have comorbidities,increasing their susceptibility to AKI.Kidney Disease:Improving Global Outcomes(KDIGO)criteria define and stage AKI based on the higher stage of AKI defined by either serum creatinine(SCr)level or urine output(UO).[2]However,the record of UO is difficult to access in clinic,the data on the value of UO combined with SCr for AKI diagnosis or prognosis in elderly patients are lacking.Therefore,the objectives of this study were to investigate whether UO would influence the classification of AKI and improve the accuracy of prediction of the clinical prognosis in elderly patients.
基金Zhou B from the Innovation Cultivation Fund of the Sixth Medical Center of PLA General Hospital(CXPY201704)the Youth Development Program of the Central Military Commission(20QNPY107).
文摘Background:Cardiorenal syndrome is increasingly common and has been reported to be associated with inflammation and oxidative stress,and statins have anti-inflammatory and antioxidant effects.Therefore,we designed this experiment to study the preventive effect of statins on cardiorenal syndrome.The aim of the study is to investigate the effect of early rosuvastatin use on cardiorenal syndrome.Method:Forty-five Wistar rats were randomly divided into 3 groups.A unilateral nephrectomy group(Group 1),a unilateral nephrectomy+coronary ligation group(Group 2),and a unilateral nephrectomy+coronary ligation+rosuvastatin group(Group 3).Right kidney removal was performed on all rats during the first week,while Group 3 was given statin intragastric administration at 10 mg/kg/d.One month later,coronary ligation was performed on rats in Groups 2 and 3.Group 3 continued statin treatment.After feeding for 3 months and 2 days,the rats were killed;urine and blood were collected and sent to the laboratory for the determination of the urinary protein/creatinine ratio and blood lipid,creatinine,and urea nitrogen levels,respectively.Serum interleukin 1β,interleukin 6,malondialdehyde,glutathione peroxidase,angiotensin II,neutrophil gelatinase-associated lipocalin,cystatin C,and B natriuretic peptide levels were also determined.On the day before euthanasia,all rats were anesthetized and examined by cardiac ultrasound.Hematoxylin-eosin and periodic acid–Schiff staining were performed on heart and kidney sections.Results:The ejection fraction in Group 2 was lower than that in Group 1(P<0.01).The ejection fraction value in Group 3 was lower than that in Group 1(P<0.01).Interleukin-1βlevels in Group 2 were higher than those in Group 1(P<0.01).Interleukin-1βlevels in Group 3 were lower than those in Group 2(P<0.01).The malondialdehyde value in Group 3 was lower than that in Group 2(P<0.05).Histopathology showed that Group 1 had slight renal damage,renal injury was aggravated in Group 2,and renal injury was still present in Group 3,but with alleviated morphology.Conclusion:The interaction of the heart and kidneys in rats is related to inflammation and oxidation.Rosuvastatin can slow down the development of the heart-kidney interaction through anti-inflammatory and antioxidant effects.
