Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury.However,whether the human dental pulp stem cell secretome can contribute to functional recovery...Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury.However,whether the human dental pulp stem cell secretome can contribute to functional recovery after spinal cord injury remains unclear.In the present study,we established a rat model of spinal cord injury based on impact injury from a dropped weight and then intraperitoneally injected the rats with conditioned medium from human dental pulp stem cells.We found that the conditioned medium effectively promoted the recovery of sensory and motor functions in rats with spinal cord injury,decreased expression of the microglial pyroptosis markers NLRP3,GSDMD,caspase-1,and interleukin-1β,promoted axonal and myelin regeneration,and inhibited the formation of glial scars.In addition,in a lipopolysaccharide-induced BV2 microglia model,conditioned medium from human dental pulp stem cells protected cells from pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway.These results indicate that conditioned medium from human dental pulp stem cells can reduce microglial pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway,thereby promoting the recovery of neurological function after spinal cord injury.Therefore,conditioned medium from human dental pulp stem cells may become an alternative therapy for spinal cord injury.展开更多
Distinctness,uniformity and stability(DUS)Test Guidelines for new varieties of Viburnum L.were developed according to the principles and requirements of the International Union for the Protection of New Varieties of P...Distinctness,uniformity and stability(DUS)Test Guidelines for new varieties of Viburnum L.were developed according to the principles and requirements of the International Union for the Protection of New Varieties of Plants(UPOV)and China.The development processes,including selection and determination of testing characteristics,determination of expression states of characteristics,selection of example varieties,determination of the order of characteristics,and assessment of distinctness,uniformity and stability,are described in this paper.There were 54 testing characteristics:24 qualitative characteristics(QL),15 quantitative characteristics(QN),15 pseudoqualitative characteristics(PQ)and 34 example varieties in the DUS Test Guidelines.Among the 54 testing characteristics,20 were asterisked and 34 were non-asterisked.Seven were selected for variety groupings and 18 were adopted in the Technical Questionnaire.The development of these DUS Test Guidelines will be useful for the application and protection of new Viburnum varieties.The Guidelines will provide technical support for DUS testing of Viburnum and serve as a reference for the development of new varieties in other countries.展开更多
The field of research on pain originating from various bone diseases is expanding rapidly, with new mechanisms and targets asserting both peripheral and central sites of action. The scope of research is broadening fro...The field of research on pain originating from various bone diseases is expanding rapidly, with new mechanisms and targets asserting both peripheral and central sites of action. The scope of research is broadening from bone biology to neuroscience,neuroendocrinology, and immunology. In particular, the roles of primary sensory neurons and non-neuronal cells in the peripheral tissues as important targets for bone pain treatment are under extensive investigation in both pre-clinical and clinical settings. An understanding of the peripheral mechanisms underlying pain conditions associated with various bone diseases will aid in the appropriate application and development of optimal strategies for not only managing bone pain symptoms but also improving bone repairing and remodeling, which potentially cures the underlying etiology for long-term functional recovery. In this review, we focus on advances in important preclinical studies of significant bone pain conditions in the past 5 years that indicated new peripheral neuronal and non-neuronal mechanisms, novel targets for potential clinical interventions, and future directions of research.展开更多
The research on the properties of graphene oxide（GO） in various media has become one of the hottest topics since GO is now the main raw material for graphene-based advanced materials. In this work, the g-ray radiati...The research on the properties of graphene oxide（GO） in various media has become one of the hottest topics since GO is now the main raw material for graphene-based advanced materials. In this work, the g-ray radiation chemistry effect of GO nanosheets and their self-aggregation behavior in t-butanol/water medium were investigated. The results show that GO nanosheets are reduced and hydroxyalkylated simultaneously by the alcohol free radicals produced by the radiolysis of t-butanol/water solution under g-ray radiation. The radiation-modified GO nanosheets will self-assemble into a self-standing graphene hydrogel when the p H of the solution is lower than 2. A hydroxyl-functionalized free-standing graphene aerogel is further obtained simply by freeze-drying. This work provides not only a general self-assembly mechanism of GO nanosheets in strong acidic alcohol/water media under high energy radiation, but also a facile and economical preparation method for hydroxyalkylated graphene-based aerogel.展开更多
基金supported by the Research Foundation of Technology Committee of Tongzhou District,No.KJ2019CX001(to SX).
文摘Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury.However,whether the human dental pulp stem cell secretome can contribute to functional recovery after spinal cord injury remains unclear.In the present study,we established a rat model of spinal cord injury based on impact injury from a dropped weight and then intraperitoneally injected the rats with conditioned medium from human dental pulp stem cells.We found that the conditioned medium effectively promoted the recovery of sensory and motor functions in rats with spinal cord injury,decreased expression of the microglial pyroptosis markers NLRP3,GSDMD,caspase-1,and interleukin-1β,promoted axonal and myelin regeneration,and inhibited the formation of glial scars.In addition,in a lipopolysaccharide-induced BV2 microglia model,conditioned medium from human dental pulp stem cells protected cells from pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway.These results indicate that conditioned medium from human dental pulp stem cells can reduce microglial pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway,thereby promoting the recovery of neurological function after spinal cord injury.Therefore,conditioned medium from human dental pulp stem cells may become an alternative therapy for spinal cord injury.
基金supported by Forestry Science&Technology Innovation Project of Shandong Province(LYCX01-2018-03)Fund for the Development of Guidelines for the Conduct of Tests for Distinctness,Uniformity and Stability(201519)
文摘Distinctness,uniformity and stability(DUS)Test Guidelines for new varieties of Viburnum L.were developed according to the principles and requirements of the International Union for the Protection of New Varieties of Plants(UPOV)and China.The development processes,including selection and determination of testing characteristics,determination of expression states of characteristics,selection of example varieties,determination of the order of characteristics,and assessment of distinctness,uniformity and stability,are described in this paper.There were 54 testing characteristics:24 qualitative characteristics(QL),15 quantitative characteristics(QN),15 pseudoqualitative characteristics(PQ)and 34 example varieties in the DUS Test Guidelines.Among the 54 testing characteristics,20 were asterisked and 34 were non-asterisked.Seven were selected for variety groupings and 18 were adopted in the Technical Questionnaire.The development of these DUS Test Guidelines will be useful for the application and protection of new Viburnum varieties.The Guidelines will provide technical support for DUS testing of Viburnum and serve as a reference for the development of new varieties in other countries.
文摘The field of research on pain originating from various bone diseases is expanding rapidly, with new mechanisms and targets asserting both peripheral and central sites of action. The scope of research is broadening from bone biology to neuroscience,neuroendocrinology, and immunology. In particular, the roles of primary sensory neurons and non-neuronal cells in the peripheral tissues as important targets for bone pain treatment are under extensive investigation in both pre-clinical and clinical settings. An understanding of the peripheral mechanisms underlying pain conditions associated with various bone diseases will aid in the appropriate application and development of optimal strategies for not only managing bone pain symptoms but also improving bone repairing and remodeling, which potentially cures the underlying etiology for long-term functional recovery. In this review, we focus on advances in important preclinical studies of significant bone pain conditions in the past 5 years that indicated new peripheral neuronal and non-neuronal mechanisms, novel targets for potential clinical interventions, and future directions of research.
基金supported by the National Natural Science Foundation of China (Nos. 51573175, 51473152, 51773189)Foshan Scientific and Technological Innovation Team Project (No. 2013IT100041)+1 种基金Foshan University-City Cooperation Project (Scientific and Technological Innovation Project, No. 2014HK100291)the Fundamental Research Funds for the Central Universities (Nos. WK2060200012, WK3450000001)
文摘The research on the properties of graphene oxide（GO） in various media has become one of the hottest topics since GO is now the main raw material for graphene-based advanced materials. In this work, the g-ray radiation chemistry effect of GO nanosheets and their self-aggregation behavior in t-butanol/water medium were investigated. The results show that GO nanosheets are reduced and hydroxyalkylated simultaneously by the alcohol free radicals produced by the radiolysis of t-butanol/water solution under g-ray radiation. The radiation-modified GO nanosheets will self-assemble into a self-standing graphene hydrogel when the p H of the solution is lower than 2. A hydroxyl-functionalized free-standing graphene aerogel is further obtained simply by freeze-drying. This work provides not only a general self-assembly mechanism of GO nanosheets in strong acidic alcohol/water media under high energy radiation, but also a facile and economical preparation method for hydroxyalkylated graphene-based aerogel.
