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EjGASA6 promotes flowering and root elongation by enhancing gibberellin biosynthesis
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作者 Qian Chen Shunyuan Yong +7 位作者 Fan Xu Hao Fu Jiangbo Dang Qiao He Danlong Jing Di Wu guolu Liang qigao guo 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第5期1568-1579,共12页
The Gibberellic Acid-stimulated Arabidopsis(GASA)gene family is involved in the regulation of gene expression and plant growth,development,and stress responses.To investigate the function of loquat GASA genes in the g... The Gibberellic Acid-stimulated Arabidopsis(GASA)gene family is involved in the regulation of gene expression and plant growth,development,and stress responses.To investigate the function of loquat GASA genes in the growth and developmental regulation of plants,a loquat EjGASA6 gene homologous to Arabidopsis AtGASA6 was cloned.EjGASA6 expression was induced by gibberellin,and ectopic transgenic plants containing this gene exhibited earlier bloom and longer primary roots since these phenotypic characteristics are related to higher gibberellin content.Transcriptome analysis and qRT-PCR results showed that the expression levels of GA3ox1 and GA20ox1,which encode key enzymes in gibberellin biosynthesis,were significantly increased.Furthermore,we confirmed that EjGASA6 could promote the expression of GA20ox1 via the luciferase reporter system.Overall,our results suggest that EjGASA6 promotes blooming and main-root elongation by positively regulating gibberellin biosynthesis.These findings broaden our understanding of the role of GASAs in plant development and growth,and lay the groundwork for future research into the functions of EjGASA6 in regulating loquat growth and development. 展开更多
关键词 LOQUAT EjGASA6 gibberellin synthesis biological function
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Genome assembly of wild loquat (Eriobotrya japonica) and resequencing provide new insights into the genomic evolution and fruit domestication in loquat 被引量:1
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作者 Danlong Jing Xinya Liu +11 位作者 Qiao He Jiangbo Dang Ruoqian Hu Yan Xia Di Wu Shuming Wang Yin Zhang Qingqing Xia Chi Zhang Yuanhui Yu qigao guo guolu Liang 《Horticulture Research》 SCIE CSCD 2023年第2期147-159,共13页
Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved varieties.Herein,we generate the first high-quality chromosome-level genome assembly of wil... Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved varieties.Herein,we generate the first high-quality chromosome-level genome assembly of wild loquat,with 45791 predicted proteincoding genes.Analysis of comparative genomics indicated that loquat shares a common ancestor with apple and pear,and a recent whole-genome duplication event occurred in loquat prior to its divergence.Genome resequencing showed that the loquat germplasms can be distinctly classified into wild and cultivated groups,and the commercial cultivars have experienced allelic admixture.Compared with cultivated loquats,the wild loquat genome showed very few selected genomic regions and had higher levels of genetic diversity.However,whole-genome scans of selective sweeps were mainly related to fruit quality,size,and f lesh color during the domestication process.Large-scale transcriptome and metabolome analyses were further performed to identify differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in wild and cultivated loquats at various fruit development stages.Unlike those in wild loquat,the key DEGs and DAMs involved in carbohydrate metabolism,plant hormone signal transduction,f lavonoid biosynthesis,and carotenoid biosynthesis were significantly regulated in cultivated loquats during fruit development.These high-quality reference genome,resequencing,and large-scale transcriptome/metabolome data provide valuable resources for elucidating fruit domestication and molecular breeding in loquat. 展开更多
关键词 process insight breeding
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Effects of Different Fertilization Treatments on Soil, Leaf Nutrient and Fruit Quality of Citrus grandis var. longanyou 被引量:5
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作者 Rongfei Li Yaodong Chang +5 位作者 Tao Hu Xueyou Jiang guolu Liang Zhiming Lu Youwen Yi qigao guo 《World Journal of Engineering and Technology》 2017年第2期1-14,共14页
The five-year-old “Longanyou” trees were used as the experimental material to study the effects of different fertilization treatments. The nutrient contents in soil and leaves, fruit yield and quality were determine... The five-year-old “Longanyou” trees were used as the experimental material to study the effects of different fertilization treatments. The nutrient contents in soil and leaves, fruit yield and quality were determined, and then the correlations were analyzed. The results showed that: 1) The soil nutrient contents of 0 - 20 cm depth were more than the 20 - 40 cm, and the trends of nutrient contents of the 0 - 20 cm soil layers were as follows: treatment 2 (T2) > treatment 3 (T3) > treatment 4 (T4) > treatment 1(T1) > control (CK). However, the 20 - 40 cm depth had not significant difference between different treatments, but T2, T4 and T3 were higher than T1 and CK. It indicated that the soil effective nutrient content increased in T2 and T3. 2) Compared with the control, the content of K and B elements was improved obviously in leaves with the increase of organic manure application. The contents of P (1.60 g·kg-1), B (26.00 mg·kg-1) and Mg (1.18 g·kg-1) were the highest, and other nutrients contents were also higher, indicating that T2 could effectively improve the leaves’ nutrient contents. 3) The fruit yield per plant was the highest in T2 (95.40 kg plant-1), and the single fruit weight, total sugar, sugar and acid ratio, vitamin C were also the highest, but titratable acid was lower. It indicated that T2 effectively improved fruit yield and quality. 4) There were positive correlations between multiple factors of soil nutrients and the quality index, such as fruit peel thickness, total sugar, solid acid ratio, sugar and acid ratio, Vc content and single yield etc. There was significant correlation between K, B, Zn, Fe contents and fruit yield and quality index, and the contents of B, Zn and Fe in leaves were significantly correlated with soil nutrient, indicating that the contents of K, B, Zn, Fe in soil and leaf were closely related to fruit yield and quality. In sum, the T2 was the best fertilization scheme for orchard management practice of “Longanyou”. 展开更多
关键词 CITRUS grandis VAR. longanyou FERTILIZATION SOIL NUTRIENT Leaf NUTRIENT FRUIT Quality
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Genetic Diversity Analysis of Balsam Pear(Momordica charantia L.) by ISSR Marker
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作者 Junhua JI Shasha HAN +6 位作者 Jia WANG Qiao HE Bo HE Zhengming ZENG Xiaolin LI guolu LIANG qigao guo 《Agricultural Science & Technology》 CAS 2014年第7期1073-1075,1088,共4页
[Objective] The aim was to explore the relationship between the classification and regional distribution of balsam pear. [Method] In the research, 30 varieties of balsam pear were analyzed by ISSR marker. [Result] The... [Objective] The aim was to explore the relationship between the classification and regional distribution of balsam pear. [Method] In the research, 30 varieties of balsam pear were analyzed by ISSR marker. [Result] The research showed that172 bands were amplified by 18 primers, in which, 132 bands were polymorphism and the polymorphic proportion was 76.7%. All of the samples can be divided into four categories by UPGMA analysis. [Conclusion] The results indicated that the classification of balsam pear is similar as varieties' regional distribution, which will be useful for genetic relationships analysis and parents selection in hybridization breeding. 展开更多
关键词 Balsam pear Genetic diversity ISSR
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Two haplotype-resolved genome assemblies for AAB allotriploid bananas provide insights into banana subgenome asymmetric evolution and Fusarium wilt control 被引量:1
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作者 Wen-Zhao Xie Yu-Yu Zheng +28 位作者 Weidi He Fangcheng Bi Yaoyao Li Tongxin Dou Run Zhou Yi-Xiong guo Guiming Deng Wenhui Zhang Min-Hui Yuan Pablo Sanz-Jimenez Xi-Tong Zhu Xin-Dong Xu Zu-Wen Zhou Zhi-Wei Zhou Jia-Wu Feng Siwen Liu Chunyu Li Qiaosong Yang Chunhua Hu Huijun Gao Tao Dong Jiangbo Dang qigao guo Wenguo Cai Jianwei Zhang Ganjun Yi Jia-Ming Song Ou Sheng Ling-Ling Chen 《Plant Communications》 SCIE CSCD 2024年第2期243-260,共18页
Bananas(Musa spp.)are one of the world’s most important fruit crops and play a vital role in food security for many developing countries.Most banana cultivars are triploids derived from inter-and intraspecific hybrid... Bananas(Musa spp.)are one of the world’s most important fruit crops and play a vital role in food security for many developing countries.Most banana cultivars are triploids derived from inter-and intraspecific hybrid-izations between the wild diploid ancestor species Musa acuminate(AA)and M.balbisiana(BB).We report two haplotype-resolved genome assemblies of the representative AAB-cultivated types,Plantain and Silk,and precisely characterize ancestral contributions by examining ancestry mosaics across the genome.Widespread asymmetric evolution is observed in their subgenomes,which can be linked to frequent homol-ogous exchange events.We reveal the genetic makeup of triploid banana cultivars and verify that subge-nome B is a rich source of disease resistance genes.Only 58.5%and 59.4%of Plantain and Silk genes,respectively,are present in all three haplotypes,with>50%of genes being differentially expressed alleles in different subgenomes.We observed that the number of upregulated genes in Plantain is significantly higher than that in Silk at one-week post-inoculation with Fusarium wilt tropical race 4(Foc TR4),which con-firms that Plantain can initiate defense responses faster than Silk.Additionally,we compared genomic and transcriptomic differences among the genes related to carotenoid synthesis and starch metabolism between Plantain and Silk.Our study provides resources for better understanding the genomic architecture of culti-vated bananas and has important implications for Musa genetics and breeding. 展开更多
关键词 bananas PLANTAIN Silk asymmetric evolution homologous exchange Fusarium wilt
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High-quality genome assemblies for two Australimusa bananas(Musa spp.)and insights into regulatory mechanisms of superior fiber properties
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作者 Run Zhou Shuo Wang +20 位作者 Ni Zhan Weidi He Guiming Deng Tongxin Dou Xi-Tong Zhu Wen-Zhao Xie Yu-Yu Zheng Chunhua Hu Fangcheng Bi Huijun Gao Tao Dong Siwen Liu Chunyu Li Qiaosong Yang Lingqiang Wang Jia-Ming Song Jiangbo Dang qigao guo Ganjun Yi Ling-Ling Chen Ou Sheng 《Plant Communications》 SCIE CSCD 2024年第1期245-261,共17页
Bananas(Musa spp.)are monocotyledonous plants with high genetic diversity in the Musaceae family that are cultivated mainly in tropical and subtropical countries.The fruits are a popular food,and the plants themselves... Bananas(Musa spp.)are monocotyledonous plants with high genetic diversity in the Musaceae family that are cultivated mainly in tropical and subtropical countries.The fruits are a popular food,and the plants themselves have diverse uses.Four genetic groups(genomes)are thought to have contributed to current banana cultivars:Musa acuminata(A genome),Musa balbisiana(B genome),Musa schizocarpa(S genome),and species of the Australimusa section(T genome).However,the T genome has not been effectively explored.Here,we present the high-quality TT genomes of two representative accessions,Abaca(Musa textilis),with high-quality naturalfiber,and Utafun(Musa troglodytarum,Fe’i group),with abundant b-carotene.Both the Abaca and Utafun assemblies comprise 10 pseudochromosomes,and their total genome sizes are 613 Mb and 619 Mb,respectively.Comparative genome analysis revealed that the larger size of the T genome is likely attributable to rapid expansion and slow removal of trans-posons.Compared with those of Musa AA or BB accessions or sisal(Agava sisalana),Abacafibers exhibit superior mechanical properties,mainly because of their thicker cell walls with a higher content of cellulose,lignin,and hemicellulose.Expression of MusaCesA cellulose synthesis genes peaks earlier in Abaca than in AA or BB accessions during plant development,potentially leading to earlier cellulose accumulation during secondary cell wall formation.The Abaca-specific expressed gene MusaMYB26,which is directly regulated by MusaMYB61,may be an important regulator that promotes precocious expression of secondary cell wall MusaCesAs.Furthermore,MusaWRKY2 and MusaNAC68,which appear to be involved in regulating expression of MusaLAC and MusaCAD,may at least partially explain the high accumulation of lignin in Abaca.This work contributes to a better understanding of banana domestica-tion and the diverse genetic resources in the Musaceae family,thus providing resources for Musa genetic improvement. 展开更多
关键词 Australimusa bananas T genome Musaceae evolution structural variations fiber
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植物外源基因组成分鉴定方法的研究进展 被引量:2
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作者 谢中艺 党江波 +3 位作者 温国 王海燕 郭启高 梁国鲁 《生物工程学报》 CAS CSCD 北大核心 2021年第8期2703-2718,共16页
具外源基因组成分(外源染色体/染色体片段/基因)植株是目前进行基因组学研究以及作物改良的重要材料。迄今为止,已建立了基于性状观测、染色体分析、特异蛋白、DNA序列4种鉴定外源基因组成分的策略。其中,基于DNA序列的分子标记技术是... 具外源基因组成分(外源染色体/染色体片段/基因)植株是目前进行基因组学研究以及作物改良的重要材料。迄今为止,已建立了基于性状观测、染色体分析、特异蛋白、DNA序列4种鉴定外源基因组成分的策略。其中,基于DNA序列的分子标记技术是当前鉴定外源基因组成分的主要手段,文中归纳了用于小麦、甘蓝等重要作物外源基因组成分的分子标记,且对简单重复序列(Simple sequence repeat,SSR)、插入缺失(Insertion-deletion,InDel)、单核苷酸多态性(Single nucleotide polymorphism,SNP)等9种标记进行了系统的比较。相比单一的鉴定方法,组合法更全面精准,文中对各组合法的应用情况进行统计和分析,提供了小麦族、芸薹族等作物的最佳鉴定组合。新一代分子标记InDel、SNP易实现高通量检测,对于外源渗入基因的精细定位展现了一定的优越性。此外,可以考虑一些新鉴定方法的加入,如微阵列比较基因组杂交(Microarray-based comparative genomic hybridization,array-CGH)、抑制差减杂交(Suppression subtractive hybridization,SSH)。 展开更多
关键词 性状观测 染色体分析 特异蛋白 分子标记 组合法 微阵列比较基因组杂交
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