The ionome is essential for maintaining body function and health status by participating in diverse key biological processes.Nevertheless, the distribution and utilization of ionome among different organs and how agin...The ionome is essential for maintaining body function and health status by participating in diverse key biological processes.Nevertheless, the distribution and utilization of ionome among different organs and how aging impacts the ionome leading to a decline in egg white quality remain unknown. Thus, we used inductively coupled plasma mass spectrometry(ICP-MS) to analyze 35 elements and their isotopic contents in eight organs of laying hens at 35, 72, and 100 weeks. Moreover, the magnum proteome, amino acids in egg white, and egg white quality were analyzed in laying hens at three different ages using 4D proteomics techniques, an amino acid analyzer, and an egg quality analyzer. Across the organs, we identified varying distribution patterns among macroelements(Mg24, Ca43/44, K39, and P31), transition metals(Zn64/66, Cu63/65, Fe56/57, and Mn55), and toxic elements(Pb208, Ba137, and Sr86). We observed an organ-specific aging pattern characterized by the accumulation of toxic elements(Pb208, Ba137, and Sr86) and calcification in the small intestine. Additionally, a decrease in the utilization of essential trace elements selenium(Se78/82) and manganese(Mn55) was noted in the oviduct. By analyzing ionome in tandem with egg quality, egg white amino acids, and proteome, we unveiled that the reduction of selenium and manganese concentrations in the magnum during the aging process affected amino acid metabolism, particularly tryptophan metabolism, thereby inhibiting the amino acid synthesis in the magnum. Furthermore, it accelerated the senescence of magnum cells through necroptosis activation, leading to a decline in the albumen secretion function of the magnum and subsequently reducing egg white quality.Overall, this study provides insights into the evolution of 35 elements and their isotopes across 8 organs of laying hens with age.It also reveals the elemental composition, interactions, and utilization patterns of these organs, as well as their correlation with egg white quality. The present study highlights the significance of ionome and offers a comprehensive perspective on the selection of ionome for regulating the aging of laying hens.展开更多
Methionine and its hydroxy analogue(MHA)have been shown to benefit mouse intestinal regeneration.The intestinal organoid is a good model that directly reflects the impact of certain nutrients or chemicals on intestina...Methionine and its hydroxy analogue(MHA)have been shown to benefit mouse intestinal regeneration.The intestinal organoid is a good model that directly reflects the impact of certain nutrients or chemicals on intestinal development.Here,we aimed to establish a chicken intestinal organoid culture method first and then use the model to explore the influence of methionine deficiency and MHA on intestinal organoid development.The results showed that 125-mm cell strainer exhibited the highest efficiency for chicken embryo crypt harvesting.We found that transforming growth factor-b inhibitor(A8301)supplementation promoted enterocyte differentiation at the expense of the proliferation of intestinal stem cells(ISC).The mitogen-activated protein kinase p38 inhibitor(SB202190)promoted intestinal organoid formation and enterocyte differentiation but suppressed the differentiation of enteroendocrine cells,goblet cells and Paneth cells.However,the suppression of enteroendocrine cell and Paneth cell differentiation by SB202190 was alleviated at the presence of A8301.The glycogen synthase kinase 3 inhibitor(CHIR99021),valproic acid(VPA)alone and their combination promoted chicken intestinal organoid formation and enterocyte differentiation at the expense of the expression of Paneth cells and goblet cells.Chicken serum significantly improved organoid formation,especially in the presence of A8301,SB202190,CHIR99021,and VPA,but inhibited the differentiation of Paneth cells and enteroendocrine cells.Chicken serum at a concentration of 0.25%meets the requirement of chicken intestinal organoid development,and the beneficial effect of chicken serum on chicken intestinal organoid culture could not be replaced by fetal bovine serum and insulin-like growth factor-1.Moreover,commercial mouse organoid culture medium supplemented with A8301,SB202190,CHIR99021,VPA,and chicken serum promotes chicken organoid budding.Based on the chicken intestinal organoid model,we found that methionine deficiency mimicked by cycloleucine suppressed organoid formation and organoid size,and this effect was reinforced with increased cycloleucine concentrations.Methionine hydroxy analogue promoted regeneration of ISC but decreased cell differentiation compared with the results obtained with L-methionine.In conclusion,our results provide a potentially excellent guideline for chicken intestinal organoid culture and insights into methionine function in crypt development.展开更多
The downwash airflow field is an important factor influencing the spraying performance of plant protection UAV,and the structural design of rotors directly affects the characteristics of the downwash airflow field.The...The downwash airflow field is an important factor influencing the spraying performance of plant protection UAV,and the structural design of rotors directly affects the characteristics of the downwash airflow field.Therefore,in this study,three-dimensional models of a six-rotor UAV with various inner tilt angles were established to simulate and analyze the influence of the inner tilt angle on the downwash airflow field based on the Reynolds average NS equation,RNG k-εturbulence model,etc..On this basis,a wireless wind speed acquisition system using the TCP server was developed to carry out the test through the marked points with real-time detection.The simulation results show that,the variation of inner tilt angles of the six-rotor UAV did not cause significant difference in the time dimension of the downwash airflow field,and with the change of the inner tilt angle from 0°to 8°,the distribution of downwash airflow field tended to obliquely shrink towards the central axis direction,and the amplitude of linear attenuation of airflow speed was also increased,which the difference of attenuation amplitude was 1 m/s.Besides,under the different inner tilt angle,the airflow velocity in“lead in area”was significantly greater than that in the“lead out area”,and the difference of air velocity distribution in space would affect the uniformity of droplet deposition.Through the calibration test,the measurement accuracy error of the developed system was lower than 0.3 m/s,and the adjusted R2 of the calibration fitting equation was higher than 0.99.The test and simulation values at test points from 0.2-2.3 m below the rotors exhibit the same variation trend,and the average relative error at the height of 1.1-2.3 m below the rotors and 0.2-0.8 m near the ground was within 10%and 20%,respectively.The simulation and test results were highly reliable,which could provide basis and reference for the design and optimization of plant protection drones.展开更多
基金supported by the National Key Research and Development Program of China (2022YFE0111100)the 2115 Talent Development Program of China Agricultural University。
文摘The ionome is essential for maintaining body function and health status by participating in diverse key biological processes.Nevertheless, the distribution and utilization of ionome among different organs and how aging impacts the ionome leading to a decline in egg white quality remain unknown. Thus, we used inductively coupled plasma mass spectrometry(ICP-MS) to analyze 35 elements and their isotopic contents in eight organs of laying hens at 35, 72, and 100 weeks. Moreover, the magnum proteome, amino acids in egg white, and egg white quality were analyzed in laying hens at three different ages using 4D proteomics techniques, an amino acid analyzer, and an egg quality analyzer. Across the organs, we identified varying distribution patterns among macroelements(Mg24, Ca43/44, K39, and P31), transition metals(Zn64/66, Cu63/65, Fe56/57, and Mn55), and toxic elements(Pb208, Ba137, and Sr86). We observed an organ-specific aging pattern characterized by the accumulation of toxic elements(Pb208, Ba137, and Sr86) and calcification in the small intestine. Additionally, a decrease in the utilization of essential trace elements selenium(Se78/82) and manganese(Mn55) was noted in the oviduct. By analyzing ionome in tandem with egg quality, egg white amino acids, and proteome, we unveiled that the reduction of selenium and manganese concentrations in the magnum during the aging process affected amino acid metabolism, particularly tryptophan metabolism, thereby inhibiting the amino acid synthesis in the magnum. Furthermore, it accelerated the senescence of magnum cells through necroptosis activation, leading to a decline in the albumen secretion function of the magnum and subsequently reducing egg white quality.Overall, this study provides insights into the evolution of 35 elements and their isotopes across 8 organs of laying hens with age.It also reveals the elemental composition, interactions, and utilization patterns of these organs, as well as their correlation with egg white quality. The present study highlights the significance of ionome and offers a comprehensive perspective on the selection of ionome for regulating the aging of laying hens.
基金This work was supported by the founding of the National Natural Science Foundation of China(No.32072752,No.31772620,No.31970814)the System for Poultry Production Technology,Beijing Agriculture Innovation Consortium(Project Number:BAIC04-2020)Natural Key R&D Project of China(2020YFA0113200).
文摘Methionine and its hydroxy analogue(MHA)have been shown to benefit mouse intestinal regeneration.The intestinal organoid is a good model that directly reflects the impact of certain nutrients or chemicals on intestinal development.Here,we aimed to establish a chicken intestinal organoid culture method first and then use the model to explore the influence of methionine deficiency and MHA on intestinal organoid development.The results showed that 125-mm cell strainer exhibited the highest efficiency for chicken embryo crypt harvesting.We found that transforming growth factor-b inhibitor(A8301)supplementation promoted enterocyte differentiation at the expense of the proliferation of intestinal stem cells(ISC).The mitogen-activated protein kinase p38 inhibitor(SB202190)promoted intestinal organoid formation and enterocyte differentiation but suppressed the differentiation of enteroendocrine cells,goblet cells and Paneth cells.However,the suppression of enteroendocrine cell and Paneth cell differentiation by SB202190 was alleviated at the presence of A8301.The glycogen synthase kinase 3 inhibitor(CHIR99021),valproic acid(VPA)alone and their combination promoted chicken intestinal organoid formation and enterocyte differentiation at the expense of the expression of Paneth cells and goblet cells.Chicken serum significantly improved organoid formation,especially in the presence of A8301,SB202190,CHIR99021,and VPA,but inhibited the differentiation of Paneth cells and enteroendocrine cells.Chicken serum at a concentration of 0.25%meets the requirement of chicken intestinal organoid development,and the beneficial effect of chicken serum on chicken intestinal organoid culture could not be replaced by fetal bovine serum and insulin-like growth factor-1.Moreover,commercial mouse organoid culture medium supplemented with A8301,SB202190,CHIR99021,VPA,and chicken serum promotes chicken organoid budding.Based on the chicken intestinal organoid model,we found that methionine deficiency mimicked by cycloleucine suppressed organoid formation and organoid size,and this effect was reinforced with increased cycloleucine concentrations.Methionine hydroxy analogue promoted regeneration of ISC but decreased cell differentiation compared with the results obtained with L-methionine.In conclusion,our results provide a potentially excellent guideline for chicken intestinal organoid culture and insights into methionine function in crypt development.
基金supported by National Natural Science Foundation of China(Grant No.31801783).
文摘The downwash airflow field is an important factor influencing the spraying performance of plant protection UAV,and the structural design of rotors directly affects the characteristics of the downwash airflow field.Therefore,in this study,three-dimensional models of a six-rotor UAV with various inner tilt angles were established to simulate and analyze the influence of the inner tilt angle on the downwash airflow field based on the Reynolds average NS equation,RNG k-εturbulence model,etc..On this basis,a wireless wind speed acquisition system using the TCP server was developed to carry out the test through the marked points with real-time detection.The simulation results show that,the variation of inner tilt angles of the six-rotor UAV did not cause significant difference in the time dimension of the downwash airflow field,and with the change of the inner tilt angle from 0°to 8°,the distribution of downwash airflow field tended to obliquely shrink towards the central axis direction,and the amplitude of linear attenuation of airflow speed was also increased,which the difference of attenuation amplitude was 1 m/s.Besides,under the different inner tilt angle,the airflow velocity in“lead in area”was significantly greater than that in the“lead out area”,and the difference of air velocity distribution in space would affect the uniformity of droplet deposition.Through the calibration test,the measurement accuracy error of the developed system was lower than 0.3 m/s,and the adjusted R2 of the calibration fitting equation was higher than 0.99.The test and simulation values at test points from 0.2-2.3 m below the rotors exhibit the same variation trend,and the average relative error at the height of 1.1-2.3 m below the rotors and 0.2-0.8 m near the ground was within 10%and 20%,respectively.The simulation and test results were highly reliable,which could provide basis and reference for the design and optimization of plant protection drones.
