Objective To study the activity of Puerarin (Pue) in scavenging oxygen free radical (OFR) and its inhibitory effect on the oxidative modification of low density lipoprotein (LDL). Methods Riboflavin-light system was...Objective To study the activity of Puerarin (Pue) in scavenging oxygen free radical (OFR) and its inhibitory effect on the oxidative modification of low density lipoprotein (LDL). Methods Riboflavin-light system was used to generate superoxide anion, and Fenton reaction to generate hydroxyl free radical to study the activity of Pue in scavenging OFR. Hydrogen peroxide-induced hemolysis was used to study the effect of Pue on erythrocyte hemolysis and malondialdehyde (MDA) production. And ultraviolet ray and cupric sulfate were used to cause the oxidative modification of LDL for studying the inhibitory effect of Pue on LDL oxidative modification. Results (1) Pue could, at concentration of 0. 01 – 1.0 mmol/L, scavenge superoxide anion radical and at concentration of 7.5–75μmol/L scavenge hydroxyl radical in a concentration dependent manner. (2) Pue could, at concentration of 0.1–10 mmol/L, inhibit significantly oxidative hemolysis and MDA production of erythrocyte induced by hydrogen peroxide. (3) Pue of 0.01–1.0 mmol/L could inhibit the oxidative modification of LDL in a concentration dependent manner. Conclusion Pue has an anti-peroxidation effect and shows a potential effect in preventing atherosclerosis.展开更多
文摘Objective To study the activity of Puerarin (Pue) in scavenging oxygen free radical (OFR) and its inhibitory effect on the oxidative modification of low density lipoprotein (LDL). Methods Riboflavin-light system was used to generate superoxide anion, and Fenton reaction to generate hydroxyl free radical to study the activity of Pue in scavenging OFR. Hydrogen peroxide-induced hemolysis was used to study the effect of Pue on erythrocyte hemolysis and malondialdehyde (MDA) production. And ultraviolet ray and cupric sulfate were used to cause the oxidative modification of LDL for studying the inhibitory effect of Pue on LDL oxidative modification. Results (1) Pue could, at concentration of 0. 01 – 1.0 mmol/L, scavenge superoxide anion radical and at concentration of 7.5–75μmol/L scavenge hydroxyl radical in a concentration dependent manner. (2) Pue could, at concentration of 0.1–10 mmol/L, inhibit significantly oxidative hemolysis and MDA production of erythrocyte induced by hydrogen peroxide. (3) Pue of 0.01–1.0 mmol/L could inhibit the oxidative modification of LDL in a concentration dependent manner. Conclusion Pue has an anti-peroxidation effect and shows a potential effect in preventing atherosclerosis.
