Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye ...Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye derivatives,designated JS016 and JS110,were produced by crossing common wheat cultivar Yangmai 23 with Pakistani rye accession W2A.Using sequential genomic in situ hybridization(GISH)and multicolor fluorescence in situ hybridization(mc-FISH),JS016 and JS110 were identified as a T6BS.6RL translocation line and a T6BS.6BL6RL translocation line,respectively.Ten newly 6RL chromosome arm-specific markers were developed and used to confirm the 6RL translocation.The wheat 55K single-nucleotide polymorphism(SNP)array further verified the molecular cytogenetic identification results above and clarified their breakpoints at 430.9 and 523.0 Mb of chromosome 6B in JS016 and JS110,respectively.Resistance spectrum and allelism test demonstrated that JS016 and JS110 possessed novel powdery mildew resistance gene(s)that was derived from the 6RL translocation but differed from Pm20.Moreover,JS016 and JS110 had better agronomic traits than the previously reported 6RL translocation line carrying Pm20.To efficiently transfer and detect the 6RL translocation from JS016 and JS110,one 6RL-specific Kompetitive allele specific PCR(KASP)marker was developed and validated in high throughput marker-assisted selection(MAS).展开更多
Effectively strengthening the surface sulfidation is essential for recovering hemimorphite by froth flotation.In this work,inductively coupled plasma optical emission spectrometer(ICP-OES)measurements,Visual MINTEQ ca...Effectively strengthening the surface sulfidation is essential for recovering hemimorphite by froth flotation.In this work,inductively coupled plasma optical emission spectrometer(ICP-OES)measurements,Visual MINTEQ calculation,X-ray photoelectron spectroscopy(XPS)analysis,time of flight secondary ion mass spectrometry(ToF-SIMS)analysis,and micro-flotation experiments were explored to systematically investigate the effect of ammonium sulfate((NH_(4))_(2)SO_(4))on the formation of zinc sulfide species on hemimorphite surface and its role in sulfidation flotation.The results showed that(NH_(4))_(2)SO_(4)exhibited a positive influence on hemimorphite sulfidation flotation.It was ascribed to the number of zinc components in the form of Zn^(2+)and[Zn(NH_(3))_(i)]^(2+)(i=1–4)increased in the flotation system after hemimorphite treatment with(NH_(4))_(2)SO_(4),which was beneficial to its interaction with sulfur species in solution,resulting in a dense and stable zinc sulfide layer generated on the hemimorphite surface.[Zn(NH_(3))_(i)]^(2+)participated in the sulfidation reaction of hemimorphite as a transition state.In addition the sulfidation reaction of hemimorphite was accelerated by(NH_(4))_(2)SO_(4).Thus,(NH_(4))_(2)SO_(4)presents a vital role in promoting the sulfidation of hemimorphite.展开更多
Koi herpes virus is a new virus found in the aquaculture production of Cryprinus carpiod and common carp in recent years. Currently,virus isolation and identification is still the traditional method for the detection ...Koi herpes virus is a new virus found in the aquaculture production of Cryprinus carpiod and common carp in recent years. Currently,virus isolation and identification is still the traditional method for the detection of Koi herpes virus,while molecular biology detection method has become the current developmental direction due to its characteristics of more sensitive,specific and rapid. Furthermore,people are still committed to exploring new detection methods for the detection of Koi herpes virus. In this paper,traditional and newly-developed detection methods of Koi herpes virus in recent years were summarized,in order to provide reference for further exploring rapid and accurate diagnostic detection method.展开更多
[Objective] This study aimed to establish a rapid and effective quarantine method of Koi herpes virus. [Method] Primers and corresponding TaqMan probe were designed based on the conserved sequence of Koi herpes virus ...[Objective] This study aimed to establish a rapid and effective quarantine method of Koi herpes virus. [Method] Primers and corresponding TaqMan probe were designed based on the conserved sequence of Koi herpes virus (KHV) polymerase gene (Sph) to establish a rapid and effective fluorescence quantitative PCR method for Koi herpes virus detection. The cell cultures were detected by using the established fluorescence quantitative PCR assay, and the results were compared with that of conventional PCR. [Result] The sensitivity of fluorescence quantitative PCR was higher than that of conventional PCR. The minimum copy number that could be detected was 1.6×102 copies/μl. The established method was adopted for sample detection, and a reliable diagnostic result could be obtained within 4 h. [Conclusion] The established method is rapid, sensitive, specific and repeatable, which is conducive to the rapid detection of Koi herpes virus.展开更多
Sophoridine and oxysophoridine are alkaloids extracted from Chinese traditional medicinewith significant antitumor activities. Computer-aided design and preliminary structure-activity relationship studiesshowed that t...Sophoridine and oxysophoridine are alkaloids extracted from Chinese traditional medicinewith significant antitumor activities. Computer-aided design and preliminary structure-activity relationship studiesshowed that the biological activity of sophoridine can be improved after oxidation (oxysophoridine) whileits toxicity significantly decreased. A new method to prepare oxysophoridine from natural product Sophoridineby biotransformation was established for the first time except for traditional extraction from plants and chemicalsynthesis. Positive biocatalytic reaction was detected by TLC. Structure of conversion product was elucidatedbased on NMR spectroscopy. Optimizations of reactions including substrate concentration, pH, conversion timeand inoculation quantity were complemented which analyzed by LC-MS. Results showed that filamentous fungusXylariales. sp F005 (CCTCC M2014660) has the capacity of synthetizing oxysophoridine from sophoridinein the selected 37 strains. The yield of oxysophoridine reached highest at conditions pH 6.0, the third day ofconversion time, 5.0 mg substrate and 5% inoculums respectively. The method for preparing oxysophoridinecatalyzed by enzymes with green and sustainable synthetic processes was developed.展开更多
Protein-biomolecule interactions play pivotal roles in almost all biological processes.For a biomolecule of interest,the identification of the interacting protein(s)is essential.For this need,although many assays are ...Protein-biomolecule interactions play pivotal roles in almost all biological processes.For a biomolecule of interest,the identification of the interacting protein(s)is essential.For this need,although many assays are available,highly robust and reliable methods are always desired.By combining a substrate-based proximity labeling activity from the pupylation pathway of Mycobacterium tuberculosis and the streptavidin(SA)-biotin system,we developed the Specific Pupylation as IDEntity Reporter(SPIDER)method for identifying protein-biomolecule interactions.Using SPIDER,we validated the interactions between the known binding proteins of protein,DNA,RNA,and small molecule.We successfully applied SPIDER to construct the global protein interactome for m^(6)A and m RNA,identified a variety of uncharacterized m^(6)A binding proteins,and validated SRSF7 as a potential m^(6)A reader.We globally identified the binding proteins for lenalidomide and Cob B.Moreover,we identified SARS-CoV-2-specific receptors on the cell membrane.Overall,SPIDER is powerful and highly accessible for the study of proteinbiomolecule interactions.展开更多
Poor soil physical properties, serious salinization and low soil nutrients are the limiting factors for crop yield in saline-sodic soil. Long-term cattle manure application is an important measure that can affect the ...Poor soil physical properties, serious salinization and low soil nutrients are the limiting factors for crop yield in saline-sodic soil. Long-term cattle manure application is an important measure that can affect the physicochemical properties and increase the maize yield of saline-sodic soil. This experiment included five treatments according to the history of cattle manure application: a control treatment with no cattle manure (CK) and treatments with cattle manure application for 14 years (14 a), 17 years (17 a), 20 years (20 a), and 25 years (25 a). The results indicated that compared with the CK treatment, long-term cattle manure application to saline-sodic soil resulted in significant increases in soil organic matter (SOM), soil total nitrogen (TN) and available nutrients at the 0-20 cm and 20-40 cm depths (p<0.05). The soil physical properties improved significantly, and cattle manure application significantly decreased the soil bulk density (ρb) and soil density (ρd) and increased the soil total porosity (ft) and water-holding capacity (WHC). With the number of years of cattle manure application, the soil pH, electrical conductivity (EC), exchangeable sodium percentage (ESP) and sodium adsorption ratio (SAR1:5) decreased significantly, and the maize yield gradually increased over time from 8690 kg/hm2 in the CK treatment to 14 690 kg/hm2 in the 25a treatment. There were significant differences among all treatments (p<0.05). The results showed that long-term cattle manure application decreased the soil ρb and saline-alkaline properties, which was the main factor that affected the maize yield in the saline-sodic soil, especially for soil ρb.展开更多
Surface plasmon devices mounted at the end-facets of optical fibers are appealing candidates for rapid and point-of-care sensing applications,by offering a special dip-and-read operation mode.At present,these devices...Surface plasmon devices mounted at the end-facets of optical fibers are appealing candidates for rapid and point-of-care sensing applications,by offering a special dip-and-read operation mode.At present,these devices’noise-equivalent limits-of-detection lag far behind the free-space counterparts,leaving them incapable of most biosensing applications.Here we report a quasi-3D Fano resonance cavity and its fabrication method to fundamentally improve the quality factor and coupling efficiency for fiber-coupled surface plasmon resonance.In this device,the Fano resonance combines the high coupling efficiency of a Fabry-Pérot etalon and the high quality factor resonance of a plasmonic crystal cavity.The quasi-3D device was fabricated on a planar substrate and transferred to a single-mode fiber end-facet,which requires a low-adhesion yet surface-plasmon-tunneling interface between the device and the planar substrate.Such an interface was realized with a nanocap-slit unit structure,of which the plasmonic crystal was consisted.A noise-equivalent limit of detection of~10-7 RIU was experimentally obtained,allowing bovine serum albumin physical adsorption to be distinguished at ng mL-1 level concentrations.Therefore,breaking through the long-standing signal-to-noise ratio bottleneck,this work makes fiber end-facet surface plasmon devices into one of high sensitivity label-free sensing technologies.At the same time,it provides an enabling top-down fabrication technology for making 3D plasmonic structures on fiber end-facets at the nanometer scale.展开更多
Saline-sodic soil has a poor structure,low nutrient content,and excessive sodium in the western Heilongjiang Province,resulting in low crop productivity.Experimental treatments were established by applying manure to t...Saline-sodic soil has a poor structure,low nutrient content,and excessive sodium in the western Heilongjiang Province,resulting in low crop productivity.Experimental treatments were established by applying manure to the soil for 5 years,12 years,and 16 years and soil without manure application was used as a control treatment(CK).The results indicate that the application of manure significantly increased soil macroaggregates,the mean weight diameter(MWD)and the geometric mean diameter(GMD)compared to those for the CK treatment.The soil organic matter(SOM)concentration increased from 17.8 to 47.9 g/kg,the soil pH decreased from 10.18 to 7.89,and the electrical conductivity(EC)decreased from 4.92 to 0.19 dS/m.The soil exchangeable Na^(+)was decreased and exchangeable Ca^(2+)was increased in the treatments with manure application compared with the CK treatment.And a decrease in the CaCO_(3)content was observed in the treatment with manure.Water-stable aggregates(WSAs)of greater than 2.0 mm were the dominant factor driving the changes in the MWD,and WSAs of 1.0-2.0 mm were the dominant factor driving the changes in the GMD.The correlation matrix showed that the SOM and soil exchangeable Ca^(2+)concentration was positively correlated with the stability of the WSAs,while the pH,EC,and soil exchangeable Na^(+)were negatively and significantly correlated.We conclude that the long-term application of manure to saline-sodic soil can increase the proportion of soil macroaggregates and thus increase the stability of WSAs,as a result of the formation of soil macroaggregates mainly caused by the increase in the organic colloidal matter and soil exchangeable Ca^(2+),and by the decrease in soil exchangeable Na^(+).展开更多
基金supported by the National Natural Science Foundation of China(32171990 and 32072053)Key Research and Development Program of Zhenjiang(NY2021001)+4 种基金State Key Laboratory of Plant Cell and Chromosome Engineering(PCCE-KF-2021-05 and PCCE-KF-2022-07)State Key Laboratory of Crop Biology in Shandong Agricultural University(2021KF01)Natural Science Foundation of the Jiangsu Higher Education institutions of China(21KJB210004)Open Project Funding of State Key Laboratory of Crop Stress Adaptation and Improvement(CX1130A0920014)Key Research and Development Program of Shandong Province(2020CXGC010805).
文摘Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye derivatives,designated JS016 and JS110,were produced by crossing common wheat cultivar Yangmai 23 with Pakistani rye accession W2A.Using sequential genomic in situ hybridization(GISH)and multicolor fluorescence in situ hybridization(mc-FISH),JS016 and JS110 were identified as a T6BS.6RL translocation line and a T6BS.6BL6RL translocation line,respectively.Ten newly 6RL chromosome arm-specific markers were developed and used to confirm the 6RL translocation.The wheat 55K single-nucleotide polymorphism(SNP)array further verified the molecular cytogenetic identification results above and clarified their breakpoints at 430.9 and 523.0 Mb of chromosome 6B in JS016 and JS110,respectively.Resistance spectrum and allelism test demonstrated that JS016 and JS110 possessed novel powdery mildew resistance gene(s)that was derived from the 6RL translocation but differed from Pm20.Moreover,JS016 and JS110 had better agronomic traits than the previously reported 6RL translocation line carrying Pm20.To efficiently transfer and detect the 6RL translocation from JS016 and JS110,one 6RL-specific Kompetitive allele specific PCR(KASP)marker was developed and validated in high throughput marker-assisted selection(MAS).
基金Fundamental Research Funds for the Central Universities(No.2023YQTD03,2022JCCX HH09,2022YJSHH01)the Yueqi Outstanding Scholaraward of China University of Mining&Technology(Beijing)+3 种基金the National Natural Science Foundation of China(No.52274283)the National Key R&D Program of China(No.SQ2022YFC2900065)the Ordos Science&Technology Plan(No.202204&2023XM06)the Open Research Fund of State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization(No.CNMRCUKF 2107)。
文摘Effectively strengthening the surface sulfidation is essential for recovering hemimorphite by froth flotation.In this work,inductively coupled plasma optical emission spectrometer(ICP-OES)measurements,Visual MINTEQ calculation,X-ray photoelectron spectroscopy(XPS)analysis,time of flight secondary ion mass spectrometry(ToF-SIMS)analysis,and micro-flotation experiments were explored to systematically investigate the effect of ammonium sulfate((NH_(4))_(2)SO_(4))on the formation of zinc sulfide species on hemimorphite surface and its role in sulfidation flotation.The results showed that(NH_(4))_(2)SO_(4)exhibited a positive influence on hemimorphite sulfidation flotation.It was ascribed to the number of zinc components in the form of Zn^(2+)and[Zn(NH_(3))_(i)]^(2+)(i=1–4)increased in the flotation system after hemimorphite treatment with(NH_(4))_(2)SO_(4),which was beneficial to its interaction with sulfur species in solution,resulting in a dense and stable zinc sulfide layer generated on the hemimorphite surface.[Zn(NH_(3))_(i)]^(2+)participated in the sulfidation reaction of hemimorphite as a transition state.In addition the sulfidation reaction of hemimorphite was accelerated by(NH_(4))_(2)SO_(4).Thus,(NH_(4))_(2)SO_(4)presents a vital role in promoting the sulfidation of hemimorphite.
基金Supported by Project of Jilin Provincial Science and Technology Commission(20080218)
文摘Koi herpes virus is a new virus found in the aquaculture production of Cryprinus carpiod and common carp in recent years. Currently,virus isolation and identification is still the traditional method for the detection of Koi herpes virus,while molecular biology detection method has become the current developmental direction due to its characteristics of more sensitive,specific and rapid. Furthermore,people are still committed to exploring new detection methods for the detection of Koi herpes virus. In this paper,traditional and newly-developed detection methods of Koi herpes virus in recent years were summarized,in order to provide reference for further exploring rapid and accurate diagnostic detection method.
基金Supported by Project of Jilin Province Science and Technology Commission(20080218)
文摘[Objective] This study aimed to establish a rapid and effective quarantine method of Koi herpes virus. [Method] Primers and corresponding TaqMan probe were designed based on the conserved sequence of Koi herpes virus (KHV) polymerase gene (Sph) to establish a rapid and effective fluorescence quantitative PCR method for Koi herpes virus detection. The cell cultures were detected by using the established fluorescence quantitative PCR assay, and the results were compared with that of conventional PCR. [Result] The sensitivity of fluorescence quantitative PCR was higher than that of conventional PCR. The minimum copy number that could be detected was 1.6×102 copies/μl. The established method was adopted for sample detection, and a reliable diagnostic result could be obtained within 4 h. [Conclusion] The established method is rapid, sensitive, specific and repeatable, which is conducive to the rapid detection of Koi herpes virus.
文摘Sophoridine and oxysophoridine are alkaloids extracted from Chinese traditional medicinewith significant antitumor activities. Computer-aided design and preliminary structure-activity relationship studiesshowed that the biological activity of sophoridine can be improved after oxidation (oxysophoridine) whileits toxicity significantly decreased. A new method to prepare oxysophoridine from natural product Sophoridineby biotransformation was established for the first time except for traditional extraction from plants and chemicalsynthesis. Positive biocatalytic reaction was detected by TLC. Structure of conversion product was elucidatedbased on NMR spectroscopy. Optimizations of reactions including substrate concentration, pH, conversion timeand inoculation quantity were complemented which analyzed by LC-MS. Results showed that filamentous fungusXylariales. sp F005 (CCTCC M2014660) has the capacity of synthetizing oxysophoridine from sophoridinein the selected 37 strains. The yield of oxysophoridine reached highest at conditions pH 6.0, the third day ofconversion time, 5.0 mg substrate and 5% inoculums respectively. The method for preparing oxysophoridinecatalyzed by enzymes with green and sustainable synthetic processes was developed.
基金supported by the National Key Research and Development Program of China(2020YFE0202200)the National Natural Science Foundation of China(31900112,21907065,31970130 and 31670831)。
文摘Protein-biomolecule interactions play pivotal roles in almost all biological processes.For a biomolecule of interest,the identification of the interacting protein(s)is essential.For this need,although many assays are available,highly robust and reliable methods are always desired.By combining a substrate-based proximity labeling activity from the pupylation pathway of Mycobacterium tuberculosis and the streptavidin(SA)-biotin system,we developed the Specific Pupylation as IDEntity Reporter(SPIDER)method for identifying protein-biomolecule interactions.Using SPIDER,we validated the interactions between the known binding proteins of protein,DNA,RNA,and small molecule.We successfully applied SPIDER to construct the global protein interactome for m^(6)A and m RNA,identified a variety of uncharacterized m^(6)A binding proteins,and validated SRSF7 as a potential m^(6)A reader.We globally identified the binding proteins for lenalidomide and Cob B.Moreover,we identified SARS-CoV-2-specific receptors on the cell membrane.Overall,SPIDER is powerful and highly accessible for the study of proteinbiomolecule interactions.
基金supported by the National Natural Science Foundation of China (Grant No.42177320).
文摘Poor soil physical properties, serious salinization and low soil nutrients are the limiting factors for crop yield in saline-sodic soil. Long-term cattle manure application is an important measure that can affect the physicochemical properties and increase the maize yield of saline-sodic soil. This experiment included five treatments according to the history of cattle manure application: a control treatment with no cattle manure (CK) and treatments with cattle manure application for 14 years (14 a), 17 years (17 a), 20 years (20 a), and 25 years (25 a). The results indicated that compared with the CK treatment, long-term cattle manure application to saline-sodic soil resulted in significant increases in soil organic matter (SOM), soil total nitrogen (TN) and available nutrients at the 0-20 cm and 20-40 cm depths (p<0.05). The soil physical properties improved significantly, and cattle manure application significantly decreased the soil bulk density (ρb) and soil density (ρd) and increased the soil total porosity (ft) and water-holding capacity (WHC). With the number of years of cattle manure application, the soil pH, electrical conductivity (EC), exchangeable sodium percentage (ESP) and sodium adsorption ratio (SAR1:5) decreased significantly, and the maize yield gradually increased over time from 8690 kg/hm2 in the CK treatment to 14 690 kg/hm2 in the 25a treatment. There were significant differences among all treatments (p<0.05). The results showed that long-term cattle manure application decreased the soil ρb and saline-alkaline properties, which was the main factor that affected the maize yield in the saline-sodic soil, especially for soil ρb.
基金supported by the National Natural Science Foundation of China(grant 61975253)the Science and Technology Commission of Shanghai Municipality(grant 21N31900200)the National Infrastructures for Translational Medicine(Shanghai) and the Lumieres(Xu Yuan)Biotechnology Company.
文摘Surface plasmon devices mounted at the end-facets of optical fibers are appealing candidates for rapid and point-of-care sensing applications,by offering a special dip-and-read operation mode.At present,these devices’noise-equivalent limits-of-detection lag far behind the free-space counterparts,leaving them incapable of most biosensing applications.Here we report a quasi-3D Fano resonance cavity and its fabrication method to fundamentally improve the quality factor and coupling efficiency for fiber-coupled surface plasmon resonance.In this device,the Fano resonance combines the high coupling efficiency of a Fabry-Pérot etalon and the high quality factor resonance of a plasmonic crystal cavity.The quasi-3D device was fabricated on a planar substrate and transferred to a single-mode fiber end-facet,which requires a low-adhesion yet surface-plasmon-tunneling interface between the device and the planar substrate.Such an interface was realized with a nanocap-slit unit structure,of which the plasmonic crystal was consisted.A noise-equivalent limit of detection of~10-7 RIU was experimentally obtained,allowing bovine serum albumin physical adsorption to be distinguished at ng mL-1 level concentrations.Therefore,breaking through the long-standing signal-to-noise ratio bottleneck,this work makes fiber end-facet surface plasmon devices into one of high sensitivity label-free sensing technologies.At the same time,it provides an enabling top-down fabrication technology for making 3D plasmonic structures on fiber end-facets at the nanometer scale.
基金This work was financially supported by the Postdoctoral Science Foundation of Heilongjiang Province(Grant No.LBH-Q19073)the“Young Talents”Project of Northeast Agricultural University(Grant No.19XG08).
文摘Saline-sodic soil has a poor structure,low nutrient content,and excessive sodium in the western Heilongjiang Province,resulting in low crop productivity.Experimental treatments were established by applying manure to the soil for 5 years,12 years,and 16 years and soil without manure application was used as a control treatment(CK).The results indicate that the application of manure significantly increased soil macroaggregates,the mean weight diameter(MWD)and the geometric mean diameter(GMD)compared to those for the CK treatment.The soil organic matter(SOM)concentration increased from 17.8 to 47.9 g/kg,the soil pH decreased from 10.18 to 7.89,and the electrical conductivity(EC)decreased from 4.92 to 0.19 dS/m.The soil exchangeable Na^(+)was decreased and exchangeable Ca^(2+)was increased in the treatments with manure application compared with the CK treatment.And a decrease in the CaCO_(3)content was observed in the treatment with manure.Water-stable aggregates(WSAs)of greater than 2.0 mm were the dominant factor driving the changes in the MWD,and WSAs of 1.0-2.0 mm were the dominant factor driving the changes in the GMD.The correlation matrix showed that the SOM and soil exchangeable Ca^(2+)concentration was positively correlated with the stability of the WSAs,while the pH,EC,and soil exchangeable Na^(+)were negatively and significantly correlated.We conclude that the long-term application of manure to saline-sodic soil can increase the proportion of soil macroaggregates and thus increase the stability of WSAs,as a result of the formation of soil macroaggregates mainly caused by the increase in the organic colloidal matter and soil exchangeable Ca^(2+),and by the decrease in soil exchangeable Na^(+).
