Using the Trial of Org 10172 in Acute Stroke Treatment (TOAST) classification for acute ischemic stroke, 371 patients with either acute large-artery atherosclerosis or small-artery oc-clusion cerebral infarction were ...Using the Trial of Org 10172 in Acute Stroke Treatment (TOAST) classification for acute ischemic stroke, 371 patients with either acute large-artery atherosclerosis or small-artery oc-clusion cerebral infarction were recruited to investigate the potential impact of elevated serum uric acid on cerebrovascular disorders. The results showed that patients who have suffered from large-artery atherosclerosis, relative to small-artery occlusion patients, were characterized by elevated serum uric acid but reduced high-density lipoprotein cholesterol and triglyceride levels. Logistic regression showed that elevated uric acid and lower triglyceride levels were the main risk factors for patients with large-artery atherosclerosis. The findings of this study suggest that hyperuricemia may be a risk factor for stroke.展开更多
This study examined a 24-year-old patient with delayed encephalopathy, who was admitted to hospital with complaints of headache and visual impairment 1 week after acute carbon monoxide poisoning. The results of a visu...This study examined a 24-year-old patient with delayed encephalopathy, who was admitted to hospital with complaints of headache and visual impairment 1 week after acute carbon monoxide poisoning. The results of a visual field assessment, electroencephalography and head magnetic resonance imaging indicated damage to the cerebral cortex. After a 2-week treatment period, the patient had recovered from the visual impairment, but exhibited digit- and letter-reading difficulty. The Chinese aphasia battery and the number and letter battery supplement were conducted. The results revealed that the patient exhibited digit and letter alexia, while the ability to read Chinese characters was preserved. In contrast, the patient exhibited a deficit in Chinese character writing, while number and letter writing remained intact. Following treatment, reading and writing ability was improved and electroencephalographic abnormalities were ameliorated. Overall, our experimental findings demonstrated that delayed encephalopathy following acute carbon monoxide poisoning was characterized by digit and letter alexia.展开更多
<正>BACKGROUND:Previous studies have shown that transplantation of vascular endothelial growth factor(VEGF)-modified neural stem cells(NSC) provides better outcomes,compared with neural stem cells,in the treatme...<正>BACKGROUND:Previous studies have shown that transplantation of vascular endothelial growth factor(VEGF)-modified neural stem cells(NSC) provides better outcomes,compared with neural stem cells,in the treatment of brain damage. OBJECTIVE:To compare the effects of VEGF-modified NSC transplantation and NSC transplantation on radiation-induced brain injury,and to determine neuron-specific enolase(NSE) expression in the brain. DESIGN,TIME,AND SETTING:The randomized,controlled study was performed at the Linbaixin Experimental Center,Second Affiliated Hospital,Sun Yat-sen University,China from November 2007 to October 2008. MATERIALS:VEGF-modified C17.2 NSCs were supplied by Harvard Medical School,USA. Streptavidin-biotin-peroxidase-complex kit(Boster,China) and 5,6-carboxyfluorescein diacetate succinimidyl ester(Fluka,USA) were used in this study. METHODS:A total of 84 Sprague Dawley rats were randomly assigned to a blank control group(n = 20),model group(n = 20),NSC group(n = 20),and a VEGF-modified NSC group(n = 24).Rat models of radiation-induced brain injury were established in the model,NSC,and VEGF-modified NSC groups.At 1 week following model induction,10μL(5×10~4 cells/μL) VEGF-modified NSCs or NSCs were respectively infused into the striatum and cerebral cortex of rats from the VEGF-modified NSC and NSC groups.A total of 10μL saline was injected into rats from the blank control and model groups. MAIN OUTCOME MEASURES:NSE expression in the brain was detected by immunohistochemistry following VEGF-modified NSC transplantation. RESULTS:NSE expression was significantly decreased in the brains of radiation-induced brain injury rats(P<0.05).The number of NSE-positive neurons significantly increased in the NSC and VEGF-modified NSC groups,compared with the model group(P<0.05).NSE expression significantly increased in the VEGF-modified NSC group,compared with the NSC group,at 6 weeks following transplantation(P<0.05). CONCLUSION:VEGF-modified NSC transplantation increased NSE expression in rats with radiation-induced brain injury,and the outcomes were superior to NSC transplantation.展开更多
Renovascular hypertensive rats (RHRs) were developed using the 2-kidney, 2-clip method. All RHRs at 10 weeks displayed high permeability of the cerebral surface blood vessels. Vascular casts of the RHRs showed that th...Renovascular hypertensive rats (RHRs) were developed using the 2-kidney, 2-clip method. All RHRs at 10 weeks displayed high permeability of the cerebral surface blood vessels. Vascular casts of the RHRs showed that the vascular network was sparse. The arterioles of the RHRs at 10 weeks had smaller lumen diameters, but thicker vessel walls with hyalinosis formation compared with control animals. The endothelial cell membrane appeared damaged, and microthrombus formed. After ischemia, the infarction size was larger in RHRs than in control animals. These results suggest that cerebral arterioles in RHRs underwent structural remodeling. High blood pressure may aggravate the severity of brain injury in cerebral ischemia and affect the recovery of ischemia.展开更多
基金the Natural Science Foundation of Guangdong Province, No. 10151130001000001, S2011010004708the Science and Technology Projects of Guangdong Province, No. 2010Y1-C191
文摘Using the Trial of Org 10172 in Acute Stroke Treatment (TOAST) classification for acute ischemic stroke, 371 patients with either acute large-artery atherosclerosis or small-artery oc-clusion cerebral infarction were recruited to investigate the potential impact of elevated serum uric acid on cerebrovascular disorders. The results showed that patients who have suffered from large-artery atherosclerosis, relative to small-artery occlusion patients, were characterized by elevated serum uric acid but reduced high-density lipoprotein cholesterol and triglyceride levels. Logistic regression showed that elevated uric acid and lower triglyceride levels were the main risk factors for patients with large-artery atherosclerosis. The findings of this study suggest that hyperuricemia may be a risk factor for stroke.
基金supported by National Natural Science Foundation of China (No. 81072242)Natural Science Foundation of Guangdong Province (No. S2011010004708)+2 种基金the Fundamental Research Funds for the Central Universities,Funds for Pearl River Science & Technology Star of Guangzhou CityNatural Science Foundation of Guangdong Province (No.10151130001000001)Science and Technology Project of Guangzhou (No. 2010Y1-C191)
文摘This study examined a 24-year-old patient with delayed encephalopathy, who was admitted to hospital with complaints of headache and visual impairment 1 week after acute carbon monoxide poisoning. The results of a visual field assessment, electroencephalography and head magnetic resonance imaging indicated damage to the cerebral cortex. After a 2-week treatment period, the patient had recovered from the visual impairment, but exhibited digit- and letter-reading difficulty. The Chinese aphasia battery and the number and letter battery supplement were conducted. The results revealed that the patient exhibited digit and letter alexia, while the ability to read Chinese characters was preserved. In contrast, the patient exhibited a deficit in Chinese character writing, while number and letter writing remained intact. Following treatment, reading and writing ability was improved and electroencephalographic abnormalities were ameliorated. Overall, our experimental findings demonstrated that delayed encephalopathy following acute carbon monoxide poisoning was characterized by digit and letter alexia.
基金Supported by:the National Natural Science Foundation of China,No.30870750the Doctor Priming Program of Natural Foundation of Guangdong Province,No. 8451008901000672+1 种基金the Medical Scientific Research Foundation Program of Guangdong Province,No. B2008044the Youth Teacher Foundation Program of Sun Yat-sen University, No,3177915
文摘<正>BACKGROUND:Previous studies have shown that transplantation of vascular endothelial growth factor(VEGF)-modified neural stem cells(NSC) provides better outcomes,compared with neural stem cells,in the treatment of brain damage. OBJECTIVE:To compare the effects of VEGF-modified NSC transplantation and NSC transplantation on radiation-induced brain injury,and to determine neuron-specific enolase(NSE) expression in the brain. DESIGN,TIME,AND SETTING:The randomized,controlled study was performed at the Linbaixin Experimental Center,Second Affiliated Hospital,Sun Yat-sen University,China from November 2007 to October 2008. MATERIALS:VEGF-modified C17.2 NSCs were supplied by Harvard Medical School,USA. Streptavidin-biotin-peroxidase-complex kit(Boster,China) and 5,6-carboxyfluorescein diacetate succinimidyl ester(Fluka,USA) were used in this study. METHODS:A total of 84 Sprague Dawley rats were randomly assigned to a blank control group(n = 20),model group(n = 20),NSC group(n = 20),and a VEGF-modified NSC group(n = 24).Rat models of radiation-induced brain injury were established in the model,NSC,and VEGF-modified NSC groups.At 1 week following model induction,10μL(5×10~4 cells/μL) VEGF-modified NSCs or NSCs were respectively infused into the striatum and cerebral cortex of rats from the VEGF-modified NSC and NSC groups.A total of 10μL saline was injected into rats from the blank control and model groups. MAIN OUTCOME MEASURES:NSE expression in the brain was detected by immunohistochemistry following VEGF-modified NSC transplantation. RESULTS:NSE expression was significantly decreased in the brains of radiation-induced brain injury rats(P<0.05).The number of NSE-positive neurons significantly increased in the NSC and VEGF-modified NSC groups,compared with the model group(P<0.05).NSE expression significantly increased in the VEGF-modified NSC group,compared with the NSC group,at 6 weeks following transplantation(P<0.05). CONCLUSION:VEGF-modified NSC transplantation increased NSE expression in rats with radiation-induced brain injury,and the outcomes were superior to NSC transplantation.
基金the Natural Science Foundation of Guangdong Province, No. 10151130001000001, S2011010004708the Science and Technology Projects of Guangdong Province, No. 2010Y1-C191
文摘Renovascular hypertensive rats (RHRs) were developed using the 2-kidney, 2-clip method. All RHRs at 10 weeks displayed high permeability of the cerebral surface blood vessels. Vascular casts of the RHRs showed that the vascular network was sparse. The arterioles of the RHRs at 10 weeks had smaller lumen diameters, but thicker vessel walls with hyalinosis formation compared with control animals. The endothelial cell membrane appeared damaged, and microthrombus formed. After ischemia, the infarction size was larger in RHRs than in control animals. These results suggest that cerebral arterioles in RHRs underwent structural remodeling. High blood pressure may aggravate the severity of brain injury in cerebral ischemia and affect the recovery of ischemia.
