Aeromonas salmonicida is a common pathogen of salmonid fishes that poses a significant threat to the fresh water and marine culture industry,potentially resulting in huge economic losses.To prevent and control fish di...Aeromonas salmonicida is a common pathogen of salmonid fishes that poses a significant threat to the fresh water and marine culture industry,potentially resulting in huge economic losses.To prevent and control fish diseases caused by A.salmonicida,rapid and effective diagnostic approaches must be developed,and which are important for routine monitoring and clinical care.By combining recombinase polymerase amplification(RPA)technology with a visible lateral flow strip(RPA-LF),we have enhanced both the precision of RPA detection and the convenience of real-time monitoring.In this study,we introduce a robust method for detecting A.salmonicida using RPA-LF.This assay specifically targets the ASA_1441 gene of A.salmonicida,ensuring high specificity,without cross-reactivity with other prevalent fresh water or marine pathogens.The optimal amplification temperature of the RPA assay was 39℃.Its sensitivity extends to as low as 100 fg of purified DNA,representing more than 1000-fold higher sensitivity than conventional PCR methods.Furthermore,to enhance the usability of the RPA-LF assay,we developed a rapid sample preparation method using cellulose dipsticks for nucleic acid extraction.This method achieves a limit of detection(LOD)as low as 1.67 CFU/μL and completes the entire process within 20 min.In conclusion,our findings present a rapid and precise tool for monitoring A.salmonicida infection in aquaculture and marine culture.This advancement offers valuable insights for effective disease prevention and control strategies.展开更多
In vertebrates,bony fishes possess not only innate immune cells but also T and B cells that are equivalent to those in mammals.However,the precise sub-cluster of immune cells in teleost fish remains largely unknown.He...In vertebrates,bony fishes possess not only innate immune cells but also T and B cells that are equivalent to those in mammals.However,the precise sub-cluster of immune cells in teleost fish remains largely unknown.Herein,we developed a dynamic bacterial infection model in turbot(Scophthalmus maximus)and created a fish immune cell landscape(FICL)for a primary lymphoid organ(head kidney),a secondary lymphoid organ(spleen),and barrier tissues(gill and posterior intestine).Moreover,through comprehensive characterization of the expression profiles of 16 clusters,including dendritic cells-like(DCs-like),macrophages(Mos),neutrophils,NK cells,as well as 12 sub-clusters of T and B cells,we found that CD8+CTLs,CD4-CD8-T,Th17 and ILC3-2 like cells posssa bifunctional role associated with cytotoxicity and immunoregulation during bacterial infection.To our knowledge,these results could provide a useful resource for a better understanding of immune cells in teleost fish and could act as a comprehensive knowledge base for assessing the evolutionary mechanism of adaptive immunity in vertebrates.展开更多
Buried pipelines are commonly damaged when laid across strike-slip faults,always leading to some level of destruction with fault movement.The moment of the neutral axis of a pipe section was obtained by integration,an...Buried pipelines are commonly damaged when laid across strike-slip faults,always leading to some level of destruction with fault movement.The moment of the neutral axis of a pipe section was obtained by integration,and the equations for the bending moment and the bending strain on a pipeline’s section were presented here,based on a trilinear stress–strain model.The calculation method for the lateral soil pressure and pipeline’s bending strain near the fault-crossing point was improved.Lateral soil pressure was regarded as being related to lateral pipeline movement and,for accurate calculation,this part of the pipeline was divided into finite element segments.An iterative process for solving for pipe bending strain was derived,and the algorithm and program for calculating bending strain and the potential damage position of the pipeline’s sections was compiled based on Matlab software.Compared with finite element method(FEM)results and the current standard method,in the situation of a pipeline being in tension under the action of a strike-slip fault(intersection angle<90),the result of the proposed method is in good agreement with FEM results.This shows that the proposed analytical method possessed a good reference value for the strain response analysis of tensile steel pipelines under strike-slip faulting.展开更多
Alzheimer's disease(AD)is an irreversible neurodegenerative disorder,which is pathologically characterized by the deposits of β-amyloid(Aβ),and plays an important role in neuronal death.Indirubin-30-monoxime(I3M...Alzheimer's disease(AD)is an irreversible neurodegenerative disorder,which is pathologically characterized by the deposits of β-amyloid(Aβ),and plays an important role in neuronal death.Indirubin-30-monoxime(I3M)showed neuroprotective effects against Aβ-induced neuronal apoptosis.However,the use of I3M in AD treatment is limited due to its low bioavailability.Herein,PLGA-PEG nanoparticles were synthesized for I3M loading.I3M could release sustainedly sustain release from the I3M-loaded PLGA-PEG nanoparticles(PLGA-PEG-I3M NPs)without obvious burst release.What's more,the PLGA-PEG-I3M NPs could significantly promote the uptake of I3M by PC12 cells through nanoparticle-mediated transport,and improve the efficacy of I3M on the inhibition of Aβfibrillization and oligomerization as well as the neuroprotective activity of I3M on Aβoligomers-induced neuronal death.Thus,the PLGA-PEG-I3M NPs may be a promising platform for AD therapy.展开更多
基金financially supported by National Key Research and Development Program of China(2022YFE0101200)National Natural Science Foundation of China(32102850)Shanghai Agricultural Science and Technology Innovation Project(T2023328).
文摘Aeromonas salmonicida is a common pathogen of salmonid fishes that poses a significant threat to the fresh water and marine culture industry,potentially resulting in huge economic losses.To prevent and control fish diseases caused by A.salmonicida,rapid and effective diagnostic approaches must be developed,and which are important for routine monitoring and clinical care.By combining recombinase polymerase amplification(RPA)technology with a visible lateral flow strip(RPA-LF),we have enhanced both the precision of RPA detection and the convenience of real-time monitoring.In this study,we introduce a robust method for detecting A.salmonicida using RPA-LF.This assay specifically targets the ASA_1441 gene of A.salmonicida,ensuring high specificity,without cross-reactivity with other prevalent fresh water or marine pathogens.The optimal amplification temperature of the RPA assay was 39℃.Its sensitivity extends to as low as 100 fg of purified DNA,representing more than 1000-fold higher sensitivity than conventional PCR methods.Furthermore,to enhance the usability of the RPA-LF assay,we developed a rapid sample preparation method using cellulose dipsticks for nucleic acid extraction.This method achieves a limit of detection(LOD)as low as 1.67 CFU/μL and completes the entire process within 20 min.In conclusion,our findings present a rapid and precise tool for monitoring A.salmonicida infection in aquaculture and marine culture.This advancement offers valuable insights for effective disease prevention and control strategies.
基金supported by the National Natural Science Foundation of China[Grant Nos.32025038(Q.L.),32122090(D.Y.)]and the Natural Science Foundation of Shanghai[Grant No.20ZR1415500(D.Y.)].
文摘In vertebrates,bony fishes possess not only innate immune cells but also T and B cells that are equivalent to those in mammals.However,the precise sub-cluster of immune cells in teleost fish remains largely unknown.Herein,we developed a dynamic bacterial infection model in turbot(Scophthalmus maximus)and created a fish immune cell landscape(FICL)for a primary lymphoid organ(head kidney),a secondary lymphoid organ(spleen),and barrier tissues(gill and posterior intestine).Moreover,through comprehensive characterization of the expression profiles of 16 clusters,including dendritic cells-like(DCs-like),macrophages(Mos),neutrophils,NK cells,as well as 12 sub-clusters of T and B cells,we found that CD8+CTLs,CD4-CD8-T,Th17 and ILC3-2 like cells posssa bifunctional role associated with cytotoxicity and immunoregulation during bacterial infection.To our knowledge,these results could provide a useful resource for a better understanding of immune cells in teleost fish and could act as a comprehensive knowledge base for assessing the evolutionary mechanism of adaptive immunity in vertebrates.
基金supported by the National Natural Science Foundation of China(Grant Nos.41941019,41877285)the Fundamental Research Funds for the Central Universities(Grant Nos.300102289201,300102281724).
文摘Buried pipelines are commonly damaged when laid across strike-slip faults,always leading to some level of destruction with fault movement.The moment of the neutral axis of a pipe section was obtained by integration,and the equations for the bending moment and the bending strain on a pipeline’s section were presented here,based on a trilinear stress–strain model.The calculation method for the lateral soil pressure and pipeline’s bending strain near the fault-crossing point was improved.Lateral soil pressure was regarded as being related to lateral pipeline movement and,for accurate calculation,this part of the pipeline was divided into finite element segments.An iterative process for solving for pipe bending strain was derived,and the algorithm and program for calculating bending strain and the potential damage position of the pipeline’s sections was compiled based on Matlab software.Compared with finite element method(FEM)results and the current standard method,in the situation of a pipeline being in tension under the action of a strike-slip fault(intersection angle<90),the result of the proposed method is in good agreement with FEM results.This shows that the proposed analytical method possessed a good reference value for the strain response analysis of tensile steel pipelines under strike-slip faulting.
基金Thanks for the funding by National Natural Science Foundation of China(Grant No.81870853)Natural Science Foundation of Zhejiang Province(Grant No.LY21H090002)+6 种基金Natural Science Foundation of Ningbo(Grant No.2018A610313)Natural Science Foundation of Guangdong(Grant No.2019A1515011750,2021A1515010720)Major program of Ningbo Science and Technology Innovation 2025(Grant No.2019B1006)Ningbo municipal innovation team of life science and health(Grant No.2015C110026)Basic scientific research operating expenses of provincial universities(Grant No.SJLY2021002)Science and Technology Innovation Commission of Shenzhen(Grant No.ZDSYS20200811142600003,JCYJ20180507183036060,JCYJ20190806161409092,JCYJ20210324103012033,JCYJ20180228162928828)LiDakSum Marine Biopharmaceutical Development Fund,the K.C.Wong Magna Fund in Ningbo University and the Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province.
文摘Alzheimer's disease(AD)is an irreversible neurodegenerative disorder,which is pathologically characterized by the deposits of β-amyloid(Aβ),and plays an important role in neuronal death.Indirubin-30-monoxime(I3M)showed neuroprotective effects against Aβ-induced neuronal apoptosis.However,the use of I3M in AD treatment is limited due to its low bioavailability.Herein,PLGA-PEG nanoparticles were synthesized for I3M loading.I3M could release sustainedly sustain release from the I3M-loaded PLGA-PEG nanoparticles(PLGA-PEG-I3M NPs)without obvious burst release.What's more,the PLGA-PEG-I3M NPs could significantly promote the uptake of I3M by PC12 cells through nanoparticle-mediated transport,and improve the efficacy of I3M on the inhibition of Aβfibrillization and oligomerization as well as the neuroprotective activity of I3M on Aβoligomers-induced neuronal death.Thus,the PLGA-PEG-I3M NPs may be a promising platform for AD therapy.
