Salmonella gallinarum has shown multiple drug resistance(MDR),especially high level fluoroquinolone(FQ)resistance in recent years.To determine whether the active efflux system was responsible for high-level FQ resista...Salmonella gallinarum has shown multiple drug resistance(MDR),especially high level fluoroquinolone(FQ)resistance in recent years.To determine whether the active efflux system was responsible for high-level FQ resistance,this research studied AcrAB efflux pump in Salmonella gallinarum on molecular level.The resistant strains were induced by standard strain C79-13 with ciprofloxacin in vitro.With carbonylcyanide-p-chlorophenyl hydrazone(CCCP)as an energy inhibitor,efflux inhibition test initially showed the potential impact of efflux pump on drug resistance.Sequence analysis of acrA gene indicated that gene mutation of AcrAB efflux pump was not definitely associated with MDR and drug resistance level of Salmonella gallinarum.Detected by competitive RT-PCR,the mRNA expression of acrA and acrB genes in the resistant strains significantly increased(p<0.01)compared with that of the control strain C79-13.The mRNA expression level of acrB gene(increased from 1.6-to 2.9-folds)was consistent with that of acrA gene(increased from 1.6-to 2.8-folds),which increased with the drug resistance level.However,gene mutation of acrA gene showed no correlation with its mRNA expression level,indicating that gene mutation did not affect the expression of AcrAB pump itself.The results suggested that the overexpression rather than the gene mutation of AcrAB efflux pump was an important factor causing the high level drug resistance of Salmonella gallinarum.展开更多
This study examined users' querying behaviors based on a sample of 30 Chinese college students from Peking University. The authors designed 5 search tasks and each participant conducted two randomly selected searc...This study examined users' querying behaviors based on a sample of 30 Chinese college students from Peking University. The authors designed 5 search tasks and each participant conducted two randomly selected search tasks during the experiment. The results show that when searching for pre-designed search tasks, users often have relatively clear goals and strategies before searching. When formulating their queries, users often select words from tasks, use concrete concepts directly, or extract 'central words' or keywords. When reformulating queries, seven query reformulation types were identified from users' behaviors, i.e. broadening, narrowing, issuing new query, paralleling, changing search tools, reformulating syntax terms, and clicking on suggested queries. The results reveal that the search results and/or the contexts can also influence users' querying behaviors.展开更多
基金Supported by the Application Technology Research and Development Project in Heilongjiang Province of China(PC13S03)the Foundation of Heilongjiang Province Educational Committee of China(11541030)。
文摘Salmonella gallinarum has shown multiple drug resistance(MDR),especially high level fluoroquinolone(FQ)resistance in recent years.To determine whether the active efflux system was responsible for high-level FQ resistance,this research studied AcrAB efflux pump in Salmonella gallinarum on molecular level.The resistant strains were induced by standard strain C79-13 with ciprofloxacin in vitro.With carbonylcyanide-p-chlorophenyl hydrazone(CCCP)as an energy inhibitor,efflux inhibition test initially showed the potential impact of efflux pump on drug resistance.Sequence analysis of acrA gene indicated that gene mutation of AcrAB efflux pump was not definitely associated with MDR and drug resistance level of Salmonella gallinarum.Detected by competitive RT-PCR,the mRNA expression of acrA and acrB genes in the resistant strains significantly increased(p<0.01)compared with that of the control strain C79-13.The mRNA expression level of acrB gene(increased from 1.6-to 2.9-folds)was consistent with that of acrA gene(increased from 1.6-to 2.8-folds),which increased with the drug resistance level.However,gene mutation of acrA gene showed no correlation with its mRNA expression level,indicating that gene mutation did not affect the expression of AcrAB pump itself.The results suggested that the overexpression rather than the gene mutation of AcrAB efflux pump was an important factor causing the high level drug resistance of Salmonella gallinarum.
基金partially supported by China Scholarship Council(Grant No.:2009601175)
文摘This study examined users' querying behaviors based on a sample of 30 Chinese college students from Peking University. The authors designed 5 search tasks and each participant conducted two randomly selected search tasks during the experiment. The results show that when searching for pre-designed search tasks, users often have relatively clear goals and strategies before searching. When formulating their queries, users often select words from tasks, use concrete concepts directly, or extract 'central words' or keywords. When reformulating queries, seven query reformulation types were identified from users' behaviors, i.e. broadening, narrowing, issuing new query, paralleling, changing search tools, reformulating syntax terms, and clicking on suggested queries. The results reveal that the search results and/or the contexts can also influence users' querying behaviors.
