Objective: To analyze the characteristics and possible mechanism of lipid metabolism in pregnant rats with intestinal flora imbalance. Methods: A total of 129 sexually mature female SD rats were divided into three gro...Objective: To analyze the characteristics and possible mechanism of lipid metabolism in pregnant rats with intestinal flora imbalance. Methods: A total of 129 sexually mature female SD rats were divided into three groups: non-pregnant group (untreated healthy rats), healthy pregnant group (natural insemination pregnant rats), and pregnant microflora disorder group (pregnant rats were given mixed antibiotics by gavage to build the modeling), with 43 rats in each group. The contents of TG, LDL, HDL and TC were detected by automatic biochemical analyzer, and the contents of SCD1, PGC-1 alpha, PEPCK, ApoE and MTTP genes were detected by fluorescence quantitative PCR technology. Regression analysis was used to explore the comprehensive influence of each gene on total cholesterol expression in rats. Principal component analysis was used to explore the internal mechanism of lipid metabolism in pregnant rats with intestinal flora disorder. Results: The contents of TG, TC, LDL and HDL were compared among the three groups of rats and the differences were statistically significant (P<0.05) . The expression levels of related genes (SCD1, PGC-1, PEPCK, ApoE, MTTP) in the three groups were statistically significant (P<0.05) . SCD1 content in the non-pregnant group, healthy pregnancy group, and disordered pregnancy group was (0.92±0.12) μg/mL, (1.20±0.15)μg/mL, and (1.53±0.20) μg/mL, respectively. PGC-1 alpha content in the non-pregnant group, healthy pregnancy group, and disordered pregnancy group was (1.34±0.21) μg/mL, (0.93±0.12) micron /mL, and (0.41±0.08) μg/mL, respectively. PEPCK content in the non-pregnant group, healthy pregnancy group, and disordered pregnancy group was (0.48±0.06) μg/mL, (0.35±0.09)μg/mL, and (0.22±0.05) μg/mL, and the differences were statistically significant (P<0.05) . Multivariate linear regression analysis showed that the influence of gene content on The effect of each gene content on TC content was in order from large to small: SCD1 (OR=4.572) , PGC-1 (OR=3.387) , PEPCK (OR=3.935) , ApoE (OR=3.597) , MTTP (OR=3.096) . The principal component analysis showed that three principal components could be extracted from five related genes of lipid metabolism in pregnant rats with intestinal dysbiosis: SCD1/PEPCK pathway (contribution rate: 36.28%) , PGC-1 /ApoE pathway (contribution rate: 30.42%) , and MTTP pathway (contribution rate: 15.37%) . Conclusion: After pregnancy, blood lipids in rats are significantly increased while the imbalance of intestinal flora will lead to decreased blood lipids. The disorder of lipid metabolism in pregnant rats with intestinal flora imbalance is mainly related to the disorder of gene expression, which further affects the functions of SCD1/PEPCK, PGC-1 /ApoE and MTTP pathways.展开更多
Objective:To investigate the differential expression and clinical features of Pim1 protein in breast tissue of healthy people, mastitis and breast cancer patients.Methods:From September 2017 to September 2018, there w...Objective:To investigate the differential expression and clinical features of Pim1 protein in breast tissue of healthy people, mastitis and breast cancer patients.Methods:From September 2017 to September 2018, there were a blank group (39 normal residents), a mastitis group (39 mastitis patients), and a paracancerous tissue group (39 paracancerous tissues of breast cancer patients). Four groups of subjects in the adenocarcinoma group (39 breast cancer patients with breast cancer) were studied. To detect the difference of Pim1 protein expression levels in breast tissue of four groups of subjects, and analyze the effect of clinical condition on the expression of Pim1 protein in breast cancer patients.Results:Compared with the blank group, the difference of Pim1 protein expression between the mastitis group, the adenocarcinoma group and the paracancerous group was statistically significant. There was a statistically significant difference in Pim1 protein expression between the paracancerous tissue group and the adenocarcinoma tissue group. Pim1 protein expression is associated with lymph node metastasis, TNM stage, and ER expression in breast cancer patients, regardless of age, histological grade, PR, and Her-2. Pim1 protein expression was evaluated as a dependent variable in breast cancer patients. Lymph node metastasis, TNM staging, and ER were independent variables. The main factors affecting Pim1 protein expression in breast cancer patients were: ER expression (OR=4.759), lymph node metastasis (OR= 3.127), TNM staging (OR = 2.664).Conclusions: Pim1 protein is closely related to the occurrence and development of mastitis and breast cancer. The detection of this index can assess the status of breast inflammation and the risk of cancer in a certain degree, and it has certain reference value for early diagnosis of breast cancer.展开更多
基金supported by the Research-Based Learning and Innovation Experimental Project for College Students in Hunan Province in 2018(Grant No.255-1097)Research-Based Learning and Innovation Experimental Project for Students in Changsha Medical University(Grant No.77-264).
文摘Objective: To analyze the characteristics and possible mechanism of lipid metabolism in pregnant rats with intestinal flora imbalance. Methods: A total of 129 sexually mature female SD rats were divided into three groups: non-pregnant group (untreated healthy rats), healthy pregnant group (natural insemination pregnant rats), and pregnant microflora disorder group (pregnant rats were given mixed antibiotics by gavage to build the modeling), with 43 rats in each group. The contents of TG, LDL, HDL and TC were detected by automatic biochemical analyzer, and the contents of SCD1, PGC-1 alpha, PEPCK, ApoE and MTTP genes were detected by fluorescence quantitative PCR technology. Regression analysis was used to explore the comprehensive influence of each gene on total cholesterol expression in rats. Principal component analysis was used to explore the internal mechanism of lipid metabolism in pregnant rats with intestinal flora disorder. Results: The contents of TG, TC, LDL and HDL were compared among the three groups of rats and the differences were statistically significant (P<0.05) . The expression levels of related genes (SCD1, PGC-1, PEPCK, ApoE, MTTP) in the three groups were statistically significant (P<0.05) . SCD1 content in the non-pregnant group, healthy pregnancy group, and disordered pregnancy group was (0.92±0.12) μg/mL, (1.20±0.15)μg/mL, and (1.53±0.20) μg/mL, respectively. PGC-1 alpha content in the non-pregnant group, healthy pregnancy group, and disordered pregnancy group was (1.34±0.21) μg/mL, (0.93±0.12) micron /mL, and (0.41±0.08) μg/mL, respectively. PEPCK content in the non-pregnant group, healthy pregnancy group, and disordered pregnancy group was (0.48±0.06) μg/mL, (0.35±0.09)μg/mL, and (0.22±0.05) μg/mL, and the differences were statistically significant (P<0.05) . Multivariate linear regression analysis showed that the influence of gene content on The effect of each gene content on TC content was in order from large to small: SCD1 (OR=4.572) , PGC-1 (OR=3.387) , PEPCK (OR=3.935) , ApoE (OR=3.597) , MTTP (OR=3.096) . The principal component analysis showed that three principal components could be extracted from five related genes of lipid metabolism in pregnant rats with intestinal dysbiosis: SCD1/PEPCK pathway (contribution rate: 36.28%) , PGC-1 /ApoE pathway (contribution rate: 30.42%) , and MTTP pathway (contribution rate: 15.37%) . Conclusion: After pregnancy, blood lipids in rats are significantly increased while the imbalance of intestinal flora will lead to decreased blood lipids. The disorder of lipid metabolism in pregnant rats with intestinal flora imbalance is mainly related to the disorder of gene expression, which further affects the functions of SCD1/PEPCK, PGC-1 /ApoE and MTTP pathways.
文摘Objective:To investigate the differential expression and clinical features of Pim1 protein in breast tissue of healthy people, mastitis and breast cancer patients.Methods:From September 2017 to September 2018, there were a blank group (39 normal residents), a mastitis group (39 mastitis patients), and a paracancerous tissue group (39 paracancerous tissues of breast cancer patients). Four groups of subjects in the adenocarcinoma group (39 breast cancer patients with breast cancer) were studied. To detect the difference of Pim1 protein expression levels in breast tissue of four groups of subjects, and analyze the effect of clinical condition on the expression of Pim1 protein in breast cancer patients.Results:Compared with the blank group, the difference of Pim1 protein expression between the mastitis group, the adenocarcinoma group and the paracancerous group was statistically significant. There was a statistically significant difference in Pim1 protein expression between the paracancerous tissue group and the adenocarcinoma tissue group. Pim1 protein expression is associated with lymph node metastasis, TNM stage, and ER expression in breast cancer patients, regardless of age, histological grade, PR, and Her-2. Pim1 protein expression was evaluated as a dependent variable in breast cancer patients. Lymph node metastasis, TNM staging, and ER were independent variables. The main factors affecting Pim1 protein expression in breast cancer patients were: ER expression (OR=4.759), lymph node metastasis (OR= 3.127), TNM staging (OR = 2.664).Conclusions: Pim1 protein is closely related to the occurrence and development of mastitis and breast cancer. The detection of this index can assess the status of breast inflammation and the risk of cancer in a certain degree, and it has certain reference value for early diagnosis of breast cancer.
