The brown seaweed,Sacchairna japonica,has been used in traditional Chinese medicine for over one thousand years.Oral administration of fucoidan or low molecular weight fucoidan(LMWF)from S.japonica could ameliorate ki...The brown seaweed,Sacchairna japonica,has been used in traditional Chinese medicine for over one thousand years.Oral administration of fucoidan or low molecular weight fucoidan(LMWF)from S.japonica could ameliorate kidney dysfunction in chronic kidney diseases and inhibit diabetic vascular complications.In many studies,LMWF was found to be more potent than fucoidan with high molecular weight.However,the pharmacokinetics of LMWF still remains unclear.The purpose of the research is to compare the pharmacokinetics of fucoidan with high molecular weight(136 kDa)with that low molecular weight(9.5 kDa)after oral administration to ICR mice.Since fucose is the main and representative monosaccharide of fucoidans,we evaluate the pharmacokinetics of fucoidan and LMWF by determining the fucose concentration in mice serum.Both fucoidan and LMWF were absorbed following oral administration.Fucoidan and LMWF were provided to mice by oral administration with 60 mg/kg and the maximum Concentration(C_(max))was found at 2.5 h(0.66±0.32 mg/L)for Fucoidan and 1.5 h(1.01±0.56 mg/L)for LMWF,respectively.It seems that LMWF had a higher area under the curve(AUC_(0–t))and was absorbed more quickly than fucoidan.The estimated bioavailability of LMWF was28.3%in the mice treated with a single dose of 30 mg/kg.In addition,LMWF was found widely spreaded into different tissues following oral administration and the highest concentration was found in kidney at 19.93±7.02μg/g.In this study,we first studied the pharmacokinetics of LMWF,in order to help to understand the function of LMWF.And our results shed light on the potential of development of drugs based on LMWF.展开更多
Pyropia yezoensis is one of the most economical seaweed species in China.Determining how to make full use of mature P.yezoensis and exploring new ways to increase the value of its resources are important subjects of r...Pyropia yezoensis is one of the most economical seaweed species in China.Determining how to make full use of mature P.yezoensis and exploring new ways to increase the value of its resources are important subjects of research for the development of the laver breeding industry.In this study,we provide a simple method to comprehensively extract the bioactive substances from P.yezoensis.The characteristics,functional properties,and antioxidant activities of two types of biopolymer extract from P.yezoensis were studied and analyzed relatively.Based on the characterization of water-soluble concentrate(WSC)and alkali-soluble concentrate(ASC),obtained via chemical analysis,including Fourier Transform Infrared Spectrometer(FT-IR)and Thermal Gravimetric Analyzer(TGA),and diff erential scanning calorimetry(DSC),they both had typical polysaccharide and protein characteristics and steady composition.ASC showed higher nitrogen solubility,water holding capacity,and foaming ability.ASC could not only be used as a protein supplement,but also performed well in improving the properties of foods in terms of water holding and fat-absorption.The emulsifying activity and oil-holding capacity of WSC were observed to be higher than those of ASC.Thus,WSC has the potential to be used as an emulsifi er.Surprisingly,WSC and ASC have radical scavenging capacity in vitro,which broadens the direction of their application.展开更多
Objective This study aimed to investigate the dose calculation accuracy of individualized bulk electron density(IBED)assignment approaches for simulated magnetic resonance imaging(MRI)-only planning of thoracic tumors...Objective This study aimed to investigate the dose calculation accuracy of individualized bulk electron density(IBED)assignment approaches for simulated magnetic resonance imaging(MRI)-only planning of thoracic tumors via the use of a 3 DVH system.Methods 8 patients with thoracic cancer were included in this study.Based on standard planning CT,single-arc dynamic conformal therapy(DCT)and double-arc volumetric modulated arc therapy(VMAT)plans with a6 MV photon beam were generated as a baseline plan(Plan-CT)for each patient.The simulated MRI-only planning(Plan-IBED)was implemented by copying the Plan-CT and forcing the electron density of each region of interest to its average value and recalculating the dose distribution.A 3 DVH system was used to visualize and compare the dosimetric differences between Plan-CT and Plan-IBED,and the criteria of the 3 D-Gamma pass rate were set to 1.0%/1.0 mm.Results The maximum percentage relative deviation(MPRD)of the dosimetric parameters D2,D95,D98,and Dmean of planning tumor volumes(PTVs)between Plan-CT and Plan-IBED was less than 1.3%.The MPRD of the average dose for organs at risk(OARs)was less than 1.5%.The MPRDs of the lung V5,V20,and V30 were 1.29%,3.26%,and 2.78%,respectively.Gamma analysis revealed an averaged pass rate of>95.0%for the body,as well as between 91.9%and 98.2%for OARs.Conclusion The proposed IBED assignment in simulated MRI-only treatment planning allows for dose calculation with comparable accuracy to the baseline plan and is appropriate for thoracic tumors.展开更多
基金Supported by the National Natural Science Foundation of China (Nos.42176137,81872906)the Nantong Science and Technology Project (No.MS12021037)+2 种基金the STS Program of Chinese Academy of Sciences (No.KFJ-STS-QYZD-195)the K.C.Wong Education FoundationCAS。
文摘The brown seaweed,Sacchairna japonica,has been used in traditional Chinese medicine for over one thousand years.Oral administration of fucoidan or low molecular weight fucoidan(LMWF)from S.japonica could ameliorate kidney dysfunction in chronic kidney diseases and inhibit diabetic vascular complications.In many studies,LMWF was found to be more potent than fucoidan with high molecular weight.However,the pharmacokinetics of LMWF still remains unclear.The purpose of the research is to compare the pharmacokinetics of fucoidan with high molecular weight(136 kDa)with that low molecular weight(9.5 kDa)after oral administration to ICR mice.Since fucose is the main and representative monosaccharide of fucoidans,we evaluate the pharmacokinetics of fucoidan and LMWF by determining the fucose concentration in mice serum.Both fucoidan and LMWF were absorbed following oral administration.Fucoidan and LMWF were provided to mice by oral administration with 60 mg/kg and the maximum Concentration(C_(max))was found at 2.5 h(0.66±0.32 mg/L)for Fucoidan and 1.5 h(1.01±0.56 mg/L)for LMWF,respectively.It seems that LMWF had a higher area under the curve(AUC_(0–t))and was absorbed more quickly than fucoidan.The estimated bioavailability of LMWF was28.3%in the mice treated with a single dose of 30 mg/kg.In addition,LMWF was found widely spreaded into different tissues following oral administration and the highest concentration was found in kidney at 19.93±7.02μg/g.In this study,we first studied the pharmacokinetics of LMWF,in order to help to understand the function of LMWF.And our results shed light on the potential of development of drugs based on LMWF.
基金Supported by the National Key Research and Development Program of China(No.2019YFD0900705)the Science and Technology Project of Fujian Province(No.2017T3015)+1 种基金the Major Scientifi c&Engineering Projects of Innovation in Shandong Province(No.2019JZZY010818)the North Jiangsu Science and Technology Special Fund(No.SZYC2018012)。
文摘Pyropia yezoensis is one of the most economical seaweed species in China.Determining how to make full use of mature P.yezoensis and exploring new ways to increase the value of its resources are important subjects of research for the development of the laver breeding industry.In this study,we provide a simple method to comprehensively extract the bioactive substances from P.yezoensis.The characteristics,functional properties,and antioxidant activities of two types of biopolymer extract from P.yezoensis were studied and analyzed relatively.Based on the characterization of water-soluble concentrate(WSC)and alkali-soluble concentrate(ASC),obtained via chemical analysis,including Fourier Transform Infrared Spectrometer(FT-IR)and Thermal Gravimetric Analyzer(TGA),and diff erential scanning calorimetry(DSC),they both had typical polysaccharide and protein characteristics and steady composition.ASC showed higher nitrogen solubility,water holding capacity,and foaming ability.ASC could not only be used as a protein supplement,but also performed well in improving the properties of foods in terms of water holding and fat-absorption.The emulsifying activity and oil-holding capacity of WSC were observed to be higher than those of ASC.Thus,WSC has the potential to be used as an emulsifi er.Surprisingly,WSC and ASC have radical scavenging capacity in vitro,which broadens the direction of their application.
基金Guangzhou Special Fund for Scientific and Technological Innovation Development(Grant No.201804010297)
文摘Objective This study aimed to investigate the dose calculation accuracy of individualized bulk electron density(IBED)assignment approaches for simulated magnetic resonance imaging(MRI)-only planning of thoracic tumors via the use of a 3 DVH system.Methods 8 patients with thoracic cancer were included in this study.Based on standard planning CT,single-arc dynamic conformal therapy(DCT)and double-arc volumetric modulated arc therapy(VMAT)plans with a6 MV photon beam were generated as a baseline plan(Plan-CT)for each patient.The simulated MRI-only planning(Plan-IBED)was implemented by copying the Plan-CT and forcing the electron density of each region of interest to its average value and recalculating the dose distribution.A 3 DVH system was used to visualize and compare the dosimetric differences between Plan-CT and Plan-IBED,and the criteria of the 3 D-Gamma pass rate were set to 1.0%/1.0 mm.Results The maximum percentage relative deviation(MPRD)of the dosimetric parameters D2,D95,D98,and Dmean of planning tumor volumes(PTVs)between Plan-CT and Plan-IBED was less than 1.3%.The MPRD of the average dose for organs at risk(OARs)was less than 1.5%.The MPRDs of the lung V5,V20,and V30 were 1.29%,3.26%,and 2.78%,respectively.Gamma analysis revealed an averaged pass rate of>95.0%for the body,as well as between 91.9%and 98.2%for OARs.Conclusion The proposed IBED assignment in simulated MRI-only treatment planning allows for dose calculation with comparable accuracy to the baseline plan and is appropriate for thoracic tumors.
