Dynamic load on anchoring structures(AS)within deep roadways can result in cumulative damage and failure.This study develops an experimental device designed to test AS under triaxial loads.The device enables the inves...Dynamic load on anchoring structures(AS)within deep roadways can result in cumulative damage and failure.This study develops an experimental device designed to test AS under triaxial loads.The device enables the investigation of the mechanical response,failure mode,instability assessment criteria,and anchorage effect of AS subjected to combined cyclic dynamic-static triaxial stress paths.The results show that the peak bearing strength is positively correlated with the anchoring matrix strength,anchorage length,and edgewise compressive strength.The bearing capacity decreases significantly when the anchorage direction is severely inclined.The free face failure modes are typically transverse cracking,concave fracturing,V-shaped slipping and detachment,and spallation detachment.Besides,when the anchoring matrix strength and the anchorage length decrease while the edgewise compressive strength,loading rate,and anchorage inclination angle increase,the failure intensity rises.Instability is determined by a negative tangent modulus of the displacement-strength curve or the continued deformation increase against the general downward trend.Under cyclic loads,the driving force that breaks the rock mass along the normal vector and the rigidity of the AS are the two factors that determine roadway stability.Finally,a control measure for surrounding rock stability is proposed to reduce the internal driving force via a pressure relief method and improve the rigidity of the AS by full-length anchorage and grouting modification.展开更多
Motivated by the recent experimental work,the pressure-induced structural transition of well-known two-dimensional(2D)1T-Hf Te_(2)was investigated up to 50 GPa through the advanced CALYPSO structure search technique c...Motivated by the recent experimental work,the pressure-induced structural transition of well-known two-dimensional(2D)1T-Hf Te_(2)was investigated up to 50 GPa through the advanced CALYPSO structure search technique combined with the first-principles calculations.Our calculations suggested that the 1T-Hf Te_(2)will first transform to C2/m phase at 3.6 GPa with a volume reduction of 7.6%and then to P62m phase at 9.6 GPa with a volume collapse of 4.6%.The occurrences of 3D C2/m and P62m phases mainly originated from the enhanced Te-Te interlayer coupling and the drastic distortions of Hf-Te polyhedrons in P3m1 phase under compression.Concomitantly,the coordination number of Hf atoms increased from six in P3m1 to eight in C2/m and eventually to nine in P62m at elevated pressure.The metallic and semimetallic nature of C2/m and P62m phases were characterized,and the evidence of the reinforced covalent interactions of Te-Hf and Te-Te orbitals in these two novel high-pressure phases were manifested by the atom-projected electronic DOS and Bader charge.展开更多
AIM:To investigate the value of combined detection of circulating cell-free DNA(cfDNA),a-fetal protein(AFP) and a L-fucosidase(AFU) for diagnosis of hepatocellular carcinoma(HCC).METHODS:Serum samples from 39 HCC pati...AIM:To investigate the value of combined detection of circulating cell-free DNA(cfDNA),a-fetal protein(AFP) and a L-fucosidase(AFU) for diagnosis of hepatocellular carcinoma(HCC).METHODS:Serum samples from 39 HCC patients and 45 normal controls were collected.Branched DNA(bDNA) was used to detect the level of cfDNA,and a receiver operating characteristic curve was employed to evaluate the diagnostic sensitivity,specificity,accuracy,positive predictive value,negative predictive value,positive likelihood ratio,negative likelihood ratio and Youden index,and to assess the diagnostic efficiency and their correlations with the clinicopathological features.AFP and AFU were detected by chemiluminescence and colorimetry,respectively.The significance of combined detection of the three biomarkers was discussed.RESULTS:cfDNA level was increased in 22 of the 39 HCC samples and in 2 of the 45 normal controls.cfDNA level in HCC samples was significantly higher than that in normal controls(P < 0.05).There were significant differences in sex and extra-and intrahepatic metastasis(P < 0.05).There was no significant correlation between cfDNA,AFP and AFU in the detection of HCC.The sensitivity of combined detection of cfDNA with one marker(AFP or AFU) and cfDNA with two markers(AFP and AFU) was 71.8%,87.2% and 89.7% vs 56.4%,53.8% and 66.7% for cfDNA,AFP and AFU used alone,respectively,the difference being statistically significant(P < 0.05).CONCLUSION:Quantitative analysis of cfDNA is sensitive and feasible,and the combined detection of cfDNA with AFP or AFU or both could improve the diagnostic sensitivity for HCC.展开更多
Silicon is a preferred material in solar cells,and most of silicon allotropes have an indirect band gap.Therefore,it is important to find new direct band gap silicon.In the present work,a new direct band gap silicon a...Silicon is a preferred material in solar cells,and most of silicon allotropes have an indirect band gap.Therefore,it is important to find new direct band gap silicon.In the present work,a new direct band gap silicon allotrope of o-Si32 is discovered.The elastic constants,elastic anisotropy,phonon spectra,and electronic structure of o-Si32 are obtained using first-principles calculations.The results show that o-Si32 is mechanically and dynamically stable and is a direct semiconductor material with a band gap of 1.261 e V.展开更多
According to the study of super-resolution range-gated system, we proposed an improved system with linear plus detects. And a range function is derived by considering the shot effect noise and dark current noise. The ...According to the study of super-resolution range-gated system, we proposed an improved system with linear plus detects. And a range function is derived by considering the shot effect noise and dark current noise. The simulation shows that the improved system has a good range accuracy capability.展开更多
The nonlinear hybrid RANS/LES method has been developed considering the simulation efficiency and accuracy to simulate the hydraulic dynamic characteristics of the centrifugal pump. It has been proved that the nonline...The nonlinear hybrid RANS/LES method has been developed considering the simulation efficiency and accuracy to simulate the hydraulic dynamic characteristics of the centrifugal pump. It has been proved that the nonlinear hybrid RANS/LES method could effectively capture the unsteady flow structure and pressure pulsation in 2?D centrifugal pumps. To further investigate the hydraulic dynamic performance of a redesigned 3-D-gap drainage centrifugal pump, the nonlinear hybrid RANS/LES method is employed. Both numerical simulation and experimental results show that the hydraulic performance and pressure pulsation characteristics of the 3-D-gap drainage impeller centrifugal pump are significantly enhanced.展开更多
Leptin receptor （LepRb） signaling pathway in the hypothalamus of the forebrain controls food intake and energy expenditure in response to an altered energy state. Defects in the LepRb signaling pathway can result in...Leptin receptor （LepRb） signaling pathway in the hypothalamus of the forebrain controls food intake and energy expenditure in response to an altered energy state. Defects in the LepRb signaling pathway can result in leptin-resistance and obesity. Leucine zipper transcription factor like 1 （Lztfl1）/BBS17 is a member of the Bardet-Biedl syndrome （BBS） gene family. Human BBS patients have a wide range of pathologies including obesity. The cellular and molecular mechanisms underlying Lztfl1-regulated obesity are unknown. Here, we generated Lztfl1lf/f mouse model in which Lztfl1 can be deleted globally and in tissue-specific manner. Global Lztfl1 deficiency resulted in pleiotropic phenotypes including obesity. Lztfl1^-/- mice are hyperphagic and showed similar energy expenditure as WT littermates. The obese phenotype of Lztfl1-/- mice is caused by the loss of Lztfl1 in the brain but not in the adipocytes.Lztfl1-/- mice are leptin-resistant. Inactivation of Lztfl1 abolished phosphorylation of Stat3 in the LepRb signaling pathway in the hypothalamus upon leptin stimulation. Deletion of Lztfl1 had no effect on LepRb membrane localization. Furthermore, we observed that Lztfl1-/- mouse embryonic fibroblasts （MEFs） have significantly longer cilia than WT MEFs. We identified several proteins that potentially interact with Lztfl1. As these proteins are known to be involved in regulation of actin/cytoskeleton dynamics, we suggest that Lztfl1 may regulate leptin signaling and ciliary structure via these proteins. Our study identified Lztfl1 as a novel player in the LepRb signaling pathway in the hypothalamus that controls energy homeostasis.展开更多
In this paper, we propose a novel hybrid sp-sp2 monoclinic carbon allotrope mC12. This allotrope is a promising light metallic material, the mechanical and electronic properties of which are studied based on first-pri...In this paper, we propose a novel hybrid sp-sp2 monoclinic carbon allotrope mC12. This allotrope is a promising light metallic material, the mechanical and electronic properties of which are studied based on first-principles calculations. The structure of this new mC12 is mechanically and dynamically stable at ambient pressure and has a low equilibrium density due to its large cell volume. Furthermore, calculations of the elastic constants and moduli reveal that mC12 has a rigid mechanical property. Finally, it exhibits metallic characteristics, owing to the mixture of sp-sp2 hybrid carbon atoms.展开更多
Based on structure prediction method,the machine learning method is used instead of the density functional theory(DFT)method to predict the material properties,thereby accelerating the material search process.In this ...Based on structure prediction method,the machine learning method is used instead of the density functional theory(DFT)method to predict the material properties,thereby accelerating the material search process.In this paper,we established a data set of carbon materials by high-throughput calculation with available carbon structures obtained from the Samara Carbon Allotrope Database.We then trained a machine learning(ML)model that specifically predicts the elastic modulus(bulk modulus,shear modulus,and the Young's modulus)and confirmed that the accuracy is better than that of AFLOW-ML in predicting the elastic modulus of a carbon allotrope.We further combined our ML model with the CALYPSO code to search for new carbon structures with a high Young's modulus.A new carbon allotrope not included in the Samara Carbon Allotrope Database,named Cmcm-C24,which exhibits a hardness greater than 80 GPa,was firstly revealed.The Cmcm-C24 phase was identified as a semiconductor with a direct bandgap.The structural stability,elastic modulus,and electronic properties of the new carbon allotrope were systematically studied,and the obtained results demonstrate the feasibility of ML methods accelerating the material search process.展开更多
Algal biofilmtechnology is a new and advanced wastewater treatment method.Experimental study on removing nitrogen and phosphorus from simulated wastewater using algal biofilm under the continuous light of 3500 Lux in ...Algal biofilmtechnology is a new and advanced wastewater treatment method.Experimental study on removing nitrogen and phosphorus from simulated wastewater using algal biofilm under the continuous light of 3500 Lux in the batch and continuous systems was carried out in this paper to assess the performance of algal biofilm in removing nutrients.The results showed that the effect of removing nitrogen and phosphorus by algal biofilm was remarkable in the batch system.The removal efficiencies of total phosphorus(TP),total nitrogen(TN),ammonianitrogen(NH_(3)-N),and chemical oxygen demand(COD)reached 98.17%,86.58%,91.88%,and 97.11%,respectively.In the continuous system,hydraulic retention time(HRT)of 4 days was adopted;the effects of removing TP,TN,NH_(3)-N,and COD by algal biofilm were very stable.During a run of 24 days,the removal efficiencies of TP,TN,NH_(3)-N,and COD reached 95.38%,83.93%,82.38%,and 92.31%,respectively.This study demonstrates the feasibility of removing nitrogen and phosphorus from simulated wastewater using algal biofilm.展开更多
There are various new structures with superior performance for Heusler alloy materials that are widely used in spintronic materials,thermoelectric materials,and other fields.In this study,three new Ag-based full-Heusl...There are various new structures with superior performance for Heusler alloy materials that are widely used in spintronic materials,thermoelectric materials,and other fields.In this study,three new Ag-based full-Heusler alloys(Ag 2 TiGa,Ag 2 VGa,and Ag 2 TiTl)are proposed.The stability,mechanical properties,and electronic proper-ties of these three new Ag-based Heusler alloys were studied using first-principles calculations.The results showed that the compression resistance,shear resistance,stiffness,and elastic anisotropy of Ag 2 VGa were greater than those of Ag 2 TiGa and Ag 2 TiTl,whereas the ductility of Ag 2 TiGa and Ag 2 TiTl was better than that of Ag 2 VGa.The spin density of states of Ag 2 VGa has evident spin splitting near the Fermi level,and the total magnetic moment of the Ag 2 VGa structure is 2.28μB,which indicates that Ag 2 VGa is magnetic.These studies enrich research on Ag-based Heusler alloys and provide theoretical references for subsequent theoretical and experimental research.展开更多
The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineur...The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineurin subunit A Δ316 mutant(CNAΔ316). A series of CNB concentrations were fitted to a four-parameter equation to calculate the corresponding p NPP maximum dephosphorylation rates. Values were calculated based on biological activity references using a parallel line method. The method was then validated for accuracy, precision, linearity, linear range, sensitivity, specificity, and robustness. The recovery results were greater than 98%. Intra-plate precision was 6.7%, with inter-plate precision of 10.8%. The coefficient of determination was greater than 0.98. The linear range was 0.05–50 μg m L?1, with sensitivity of 50 μg m L?1. Tested cytokines did not induce CNAΔ316 dephosphorylation of p NPP. The chosen CNAΔ316 concentration range did not affect activity determinations.展开更多
Results demonstrate that calcineurin plays a role in long term potentiation (LTP) in the hippocampus. A 45 ku enzyme was administered, which exhibits Ca 2+/calmodulin independent activity to rats, and the protein comp...Results demonstrate that calcineurin plays a role in long term potentiation (LTP) in the hippocampus. A 45 ku enzyme was administered, which exhibits Ca 2+/calmodulin independent activity to rats, and the protein components of nuclear extracts from the cell-free system of rat hippocampus were tested. It is found that the level of a component significantly increased in nuclear extracts following the activation of calcineurin. The concentration of the component in the nucleus is also markedly increased following hippocampal LTP elicited by ginsenosides. These results suggest that the activation of calcineurin induces a pre-existing protein component to translocate to the nucleus in the hippocampus. The nuclear translocation of the component may be required for LTP in the hippocampus.展开更多
基金This paper is financially supported by the National Natural Science Foundation of China(Grant Nos.52074263 and 52034007)the Postgraduate Research and Practice Innovation Program of Jiangsu Province(Grant No.KYCX21_2332).
文摘Dynamic load on anchoring structures(AS)within deep roadways can result in cumulative damage and failure.This study develops an experimental device designed to test AS under triaxial loads.The device enables the investigation of the mechanical response,failure mode,instability assessment criteria,and anchorage effect of AS subjected to combined cyclic dynamic-static triaxial stress paths.The results show that the peak bearing strength is positively correlated with the anchoring matrix strength,anchorage length,and edgewise compressive strength.The bearing capacity decreases significantly when the anchorage direction is severely inclined.The free face failure modes are typically transverse cracking,concave fracturing,V-shaped slipping and detachment,and spallation detachment.Besides,when the anchoring matrix strength and the anchorage length decrease while the edgewise compressive strength,loading rate,and anchorage inclination angle increase,the failure intensity rises.Instability is determined by a negative tangent modulus of the displacement-strength curve or the continued deformation increase against the general downward trend.Under cyclic loads,the driving force that breaks the rock mass along the normal vector and the rigidity of the AS are the two factors that determine roadway stability.Finally,a control measure for surrounding rock stability is proposed to reduce the internal driving force via a pressure relief method and improve the rigidity of the AS by full-length anchorage and grouting modification.
基金Project supported by the National Natural Science Foundation of China(Grant No.11964026)the Natural Science Basic Research Plan in Shaanxi Province of China(Grant Nos.2023-JC-YB-021,2022JM-035,and 2022JQ-008)。
文摘Motivated by the recent experimental work,the pressure-induced structural transition of well-known two-dimensional(2D)1T-Hf Te_(2)was investigated up to 50 GPa through the advanced CALYPSO structure search technique combined with the first-principles calculations.Our calculations suggested that the 1T-Hf Te_(2)will first transform to C2/m phase at 3.6 GPa with a volume reduction of 7.6%and then to P62m phase at 9.6 GPa with a volume collapse of 4.6%.The occurrences of 3D C2/m and P62m phases mainly originated from the enhanced Te-Te interlayer coupling and the drastic distortions of Hf-Te polyhedrons in P3m1 phase under compression.Concomitantly,the coordination number of Hf atoms increased from six in P3m1 to eight in C2/m and eventually to nine in P62m at elevated pressure.The metallic and semimetallic nature of C2/m and P62m phases were characterized,and the evidence of the reinforced covalent interactions of Te-Hf and Te-Te orbitals in these two novel high-pressure phases were manifested by the atom-projected electronic DOS and Bader charge.
文摘AIM:To investigate the value of combined detection of circulating cell-free DNA(cfDNA),a-fetal protein(AFP) and a L-fucosidase(AFU) for diagnosis of hepatocellular carcinoma(HCC).METHODS:Serum samples from 39 HCC patients and 45 normal controls were collected.Branched DNA(bDNA) was used to detect the level of cfDNA,and a receiver operating characteristic curve was employed to evaluate the diagnostic sensitivity,specificity,accuracy,positive predictive value,negative predictive value,positive likelihood ratio,negative likelihood ratio and Youden index,and to assess the diagnostic efficiency and their correlations with the clinicopathological features.AFP and AFU were detected by chemiluminescence and colorimetry,respectively.The significance of combined detection of the three biomarkers was discussed.RESULTS:cfDNA level was increased in 22 of the 39 HCC samples and in 2 of the 45 normal controls.cfDNA level in HCC samples was significantly higher than that in normal controls(P < 0.05).There were significant differences in sex and extra-and intrahepatic metastasis(P < 0.05).There was no significant correlation between cfDNA,AFP and AFU in the detection of HCC.The sensitivity of combined detection of cfDNA with one marker(AFP or AFU) and cfDNA with two markers(AFP and AFU) was 71.8%,87.2% and 89.7% vs 56.4%,53.8% and 66.7% for cfDNA,AFP and AFU used alone,respectively,the difference being statistically significant(P < 0.05).CONCLUSION:Quantitative analysis of cfDNA is sensitive and feasible,and the combined detection of cfDNA with AFP or AFU or both could improve the diagnostic sensitivity for HCC.
基金supported by the National Natural Science Foundation of China(Grant Nos.11965005 and 11964026)the 111 Project,China(Grant No.B17035)+1 种基金the Natural Science Basic Research Plan in Shaanxi Province of China(Grant Nos.2020JM-186 and 2020JM-621)the Fundamental Research Funds for the Central Universities,China。
文摘Silicon is a preferred material in solar cells,and most of silicon allotropes have an indirect band gap.Therefore,it is important to find new direct band gap silicon.In the present work,a new direct band gap silicon allotrope of o-Si32 is discovered.The elastic constants,elastic anisotropy,phonon spectra,and electronic structure of o-Si32 are obtained using first-principles calculations.The results show that o-Si32 is mechanically and dynamically stable and is a direct semiconductor material with a band gap of 1.261 e V.
文摘According to the study of super-resolution range-gated system, we proposed an improved system with linear plus detects. And a range function is derived by considering the shot effect noise and dark current noise. The simulation shows that the improved system has a good range accuracy capability.
基金the National Natural Science Foundation of China (Grant Nos. 51379120, 51179100).
文摘The nonlinear hybrid RANS/LES method has been developed considering the simulation efficiency and accuracy to simulate the hydraulic dynamic characteristics of the centrifugal pump. It has been proved that the nonlinear hybrid RANS/LES method could effectively capture the unsteady flow structure and pressure pulsation in 2?D centrifugal pumps. To further investigate the hydraulic dynamic performance of a redesigned 3-D-gap drainage centrifugal pump, the nonlinear hybrid RANS/LES method is employed. Both numerical simulation and experimental results show that the hydraulic performance and pressure pulsation characteristics of the 3-D-gap drainage impeller centrifugal pump are significantly enhanced.
基金This study was supported by the National Natural Science Foundation of China （81101581 and 81672840 to Q.W.）, AHA- 16GRNT3071000, NIHRO1-HL10947, and CPRIT-RP120717 to Z.-P.L
文摘Leptin receptor （LepRb） signaling pathway in the hypothalamus of the forebrain controls food intake and energy expenditure in response to an altered energy state. Defects in the LepRb signaling pathway can result in leptin-resistance and obesity. Leucine zipper transcription factor like 1 （Lztfl1）/BBS17 is a member of the Bardet-Biedl syndrome （BBS） gene family. Human BBS patients have a wide range of pathologies including obesity. The cellular and molecular mechanisms underlying Lztfl1-regulated obesity are unknown. Here, we generated Lztfl1lf/f mouse model in which Lztfl1 can be deleted globally and in tissue-specific manner. Global Lztfl1 deficiency resulted in pleiotropic phenotypes including obesity. Lztfl1^-/- mice are hyperphagic and showed similar energy expenditure as WT littermates. The obese phenotype of Lztfl1-/- mice is caused by the loss of Lztfl1 in the brain but not in the adipocytes.Lztfl1-/- mice are leptin-resistant. Inactivation of Lztfl1 abolished phosphorylation of Stat3 in the LepRb signaling pathway in the hypothalamus upon leptin stimulation. Deletion of Lztfl1 had no effect on LepRb membrane localization. Furthermore, we observed that Lztfl1-/- mouse embryonic fibroblasts （MEFs） have significantly longer cilia than WT MEFs. We identified several proteins that potentially interact with Lztfl1. As these proteins are known to be involved in regulation of actin/cytoskeleton dynamics, we suggest that Lztfl1 may regulate leptin signaling and ciliary structure via these proteins. Our study identified Lztfl1 as a novel player in the LepRb signaling pathway in the hypothalamus that controls energy homeostasis.
基金This work was financially supported by the National Natural Science Foundation of China （Grant No. 11204007）, the 111 Project （B17035）, tile Natural Science New Star of Science and Technologies Research Plan in Shaanxi Province of China （Grant No. 2017KJXX-53）, and Education Com- mittee Natural Science Foundation in Shaanxi Province of China （Grant No. 16JK1049）. Xiao-Feng Shi is acknowledged for help- fill discussions and comments on the manuscript. All the authors thank the computing facilities at the High Performance Computing Center of Xidian University.
文摘In this paper, we propose a novel hybrid sp-sp2 monoclinic carbon allotrope mC12. This allotrope is a promising light metallic material, the mechanical and electronic properties of which are studied based on first-principles calculations. The structure of this new mC12 is mechanically and dynamically stable at ambient pressure and has a low equilibrium density due to its large cell volume. Furthermore, calculations of the elastic constants and moduli reveal that mC12 has a rigid mechanical property. Finally, it exhibits metallic characteristics, owing to the mixture of sp-sp2 hybrid carbon atoms.
基金This work was financlally supported by the Fundamental Research Funds for the Central Universities,the Na-tional Natural Science Foundation of China(Grant Nos.11965005 and 11964026)the 111 Project(No.B17035)the Natural Sci-ence Basie Research plan in Shaanxi Province of China(Grant Nos.2020JM-186 and 2020JM-621).
文摘Based on structure prediction method,the machine learning method is used instead of the density functional theory(DFT)method to predict the material properties,thereby accelerating the material search process.In this paper,we established a data set of carbon materials by high-throughput calculation with available carbon structures obtained from the Samara Carbon Allotrope Database.We then trained a machine learning(ML)model that specifically predicts the elastic modulus(bulk modulus,shear modulus,and the Young's modulus)and confirmed that the accuracy is better than that of AFLOW-ML in predicting the elastic modulus of a carbon allotrope.We further combined our ML model with the CALYPSO code to search for new carbon structures with a high Young's modulus.A new carbon allotrope not included in the Samara Carbon Allotrope Database,named Cmcm-C24,which exhibits a hardness greater than 80 GPa,was firstly revealed.The Cmcm-C24 phase was identified as a semiconductor with a direct bandgap.The structural stability,elastic modulus,and electronic properties of the new carbon allotrope were systematically studied,and the obtained results demonstrate the feasibility of ML methods accelerating the material search process.
基金The work was supported by Innovation Fund of Postgraduate of Huazhong University of Science and Technology(HUST)(No.HF-05-07-2007-261)the Opening Project of State Key Laboratory of Freshwater Ecology and Biotechnology of Chinese Academy of Sciences(No.2008FB006)HUST Brain Gain Fund(No.0124261002),China.
文摘Algal biofilmtechnology is a new and advanced wastewater treatment method.Experimental study on removing nitrogen and phosphorus from simulated wastewater using algal biofilm under the continuous light of 3500 Lux in the batch and continuous systems was carried out in this paper to assess the performance of algal biofilm in removing nutrients.The results showed that the effect of removing nitrogen and phosphorus by algal biofilm was remarkable in the batch system.The removal efficiencies of total phosphorus(TP),total nitrogen(TN),ammonianitrogen(NH_(3)-N),and chemical oxygen demand(COD)reached 98.17%,86.58%,91.88%,and 97.11%,respectively.In the continuous system,hydraulic retention time(HRT)of 4 days was adopted;the effects of removing TP,TN,NH_(3)-N,and COD by algal biofilm were very stable.During a run of 24 days,the removal efficiencies of TP,TN,NH_(3)-N,and COD reached 95.38%,83.93%,82.38%,and 92.31%,respectively.This study demonstrates the feasibility of removing nitrogen and phosphorus from simulated wastewater using algal biofilm.
基金This work was financially supported by the National Natural Sci-ence Foundation of China(Grant Nos.:11965005 and 11964026)the Natural Science Basic Research plan in Shaanxi Province of China(Grant Nos.:2020JM-186,2022JM-035,and 2022JQ-008)the Fundamen-tal Research Funds for the Central Universities.All the authors thank the computing facilities at High Performance Computing Center of Xidian University.
文摘There are various new structures with superior performance for Heusler alloy materials that are widely used in spintronic materials,thermoelectric materials,and other fields.In this study,three new Ag-based full-Heusler alloys(Ag 2 TiGa,Ag 2 VGa,and Ag 2 TiTl)are proposed.The stability,mechanical properties,and electronic proper-ties of these three new Ag-based Heusler alloys were studied using first-principles calculations.The results showed that the compression resistance,shear resistance,stiffness,and elastic anisotropy of Ag 2 VGa were greater than those of Ag 2 TiGa and Ag 2 TiTl,whereas the ductility of Ag 2 TiGa and Ag 2 TiTl was better than that of Ag 2 VGa.The spin density of states of Ag 2 VGa has evident spin splitting near the Fermi level,and the total magnetic moment of the Ag 2 VGa structure is 2.28μB,which indicates that Ag 2 VGa is magnetic.These studies enrich research on Ag-based Heusler alloys and provide theoretical references for subsequent theoretical and experimental research.
基金supported by the National Important Novel Medicine Research Project (2012ZX09304010, 2013ZX09102062)the National Natural Science Foundation of China (31270849)
文摘The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineurin subunit A Δ316 mutant(CNAΔ316). A series of CNB concentrations were fitted to a four-parameter equation to calculate the corresponding p NPP maximum dephosphorylation rates. Values were calculated based on biological activity references using a parallel line method. The method was then validated for accuracy, precision, linearity, linear range, sensitivity, specificity, and robustness. The recovery results were greater than 98%. Intra-plate precision was 6.7%, with inter-plate precision of 10.8%. The coefficient of determination was greater than 0.98. The linear range was 0.05–50 μg m L?1, with sensitivity of 50 μg m L?1. Tested cytokines did not induce CNAΔ316 dephosphorylation of p NPP. The chosen CNAΔ316 concentration range did not affect activity determinations.
文摘Results demonstrate that calcineurin plays a role in long term potentiation (LTP) in the hippocampus. A 45 ku enzyme was administered, which exhibits Ca 2+/calmodulin independent activity to rats, and the protein components of nuclear extracts from the cell-free system of rat hippocampus were tested. It is found that the level of a component significantly increased in nuclear extracts following the activation of calcineurin. The concentration of the component in the nucleus is also markedly increased following hippocampal LTP elicited by ginsenosides. These results suggest that the activation of calcineurin induces a pre-existing protein component to translocate to the nucleus in the hippocampus. The nuclear translocation of the component may be required for LTP in the hippocampus.
