The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and doco- sahexaenoic acid (C22 : 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investiga...The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and doco- sahexaenoic acid (C22 : 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investigated in the present study. The results revealed that the optimal temperature, initial pH and salinity level of the medium for DHA production were 23℃, 7.0 and 18, respectively. Glucose was proved the best carbon source for the growth and DHA production from S. limacinum. Among the nitrogen sources tested, soybean cake hydrolysate, a cheap by-product, was found to be effective for the accumulation of DHA in S. lima- cinum cells. In addition, increasing the concentration of carbon sources in the medium caused a significant increase in cell biomass; however, accumulation of DHA in cells was mainly stimulated by the ratio of C/N in the medium. Under the optimal culture condi- tions, the maximum DHA yield achieved in flasks was 4.08 g L-1 after 5 d of cultivation.展开更多
目的观察经颅直流电刺激(transcranial direct current stimulation,tDCS)在治疗儿童注意缺陷多动障碍(Attention-deficit hyperactivity disorder,ADHD)自我意识及自我控制中的作用。方法将165例ADHD患儿随机分为观察组(tDCS+盐酸哌甲...目的观察经颅直流电刺激(transcranial direct current stimulation,tDCS)在治疗儿童注意缺陷多动障碍(Attention-deficit hyperactivity disorder,ADHD)自我意识及自我控制中的作用。方法将165例ADHD患儿随机分为观察组(tDCS+盐酸哌甲酯缓释片)、对照组1(生物反馈治疗+盐酸哌甲酯缓释片)、对照组2(单纯口服盐酸哌甲酯缓释片),观察8周。采用注意缺陷多动障碍诊断量表父母版(ADHD-RS-Ⅳ)、Piers-Harris儿童自我意识量表(PHCSS)、儿童自我控制双系统量表(DSSCSC)评估治疗效果。结果干预8周末,各组与治疗前自身对比,发现ADHD-RS-Ⅳ量表各项和总分项均有下降;PHCSS量表中除焦虑项分值下降以外,其他各项分值均有升高;DSSCSC中自我控制系统项和总分项分值升高,冲动系统项分值下降;经t检验,P<0.05,差异有统计学意义;各组组间对比ADHD-RS-Ⅳ、PHCSS、DSSCSC各项评分,经方差分析,P<0.05,差异有统计学意义。两两比较,经q'检验,A-B、A-C,P<0.05,差异有统计学意义。B-C,ADHD中的注意缺陷项和总分,经q'检验,P>0.05,差异无统计学意义。提示,tDCS+盐酸哌甲酯缓释片治疗效果优于生物反馈治疗+盐酸哌甲酯缓释片治疗优于盐酸哌甲酯缓释片。结论tDCS能提高ADHD患儿的自我意识水平,促进其自我控制的能力。其结果可能与tDCS机制有关。展开更多
文摘The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and doco- sahexaenoic acid (C22 : 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investigated in the present study. The results revealed that the optimal temperature, initial pH and salinity level of the medium for DHA production were 23℃, 7.0 and 18, respectively. Glucose was proved the best carbon source for the growth and DHA production from S. limacinum. Among the nitrogen sources tested, soybean cake hydrolysate, a cheap by-product, was found to be effective for the accumulation of DHA in S. lima- cinum cells. In addition, increasing the concentration of carbon sources in the medium caused a significant increase in cell biomass; however, accumulation of DHA in cells was mainly stimulated by the ratio of C/N in the medium. Under the optimal culture condi- tions, the maximum DHA yield achieved in flasks was 4.08 g L-1 after 5 d of cultivation.
文摘目的观察经颅直流电刺激(transcranial direct current stimulation,tDCS)在治疗儿童注意缺陷多动障碍(Attention-deficit hyperactivity disorder,ADHD)自我意识及自我控制中的作用。方法将165例ADHD患儿随机分为观察组(tDCS+盐酸哌甲酯缓释片)、对照组1(生物反馈治疗+盐酸哌甲酯缓释片)、对照组2(单纯口服盐酸哌甲酯缓释片),观察8周。采用注意缺陷多动障碍诊断量表父母版(ADHD-RS-Ⅳ)、Piers-Harris儿童自我意识量表(PHCSS)、儿童自我控制双系统量表(DSSCSC)评估治疗效果。结果干预8周末,各组与治疗前自身对比,发现ADHD-RS-Ⅳ量表各项和总分项均有下降;PHCSS量表中除焦虑项分值下降以外,其他各项分值均有升高;DSSCSC中自我控制系统项和总分项分值升高,冲动系统项分值下降;经t检验,P<0.05,差异有统计学意义;各组组间对比ADHD-RS-Ⅳ、PHCSS、DSSCSC各项评分,经方差分析,P<0.05,差异有统计学意义。两两比较,经q'检验,A-B、A-C,P<0.05,差异有统计学意义。B-C,ADHD中的注意缺陷项和总分,经q'检验,P>0.05,差异无统计学意义。提示,tDCS+盐酸哌甲酯缓释片治疗效果优于生物反馈治疗+盐酸哌甲酯缓释片治疗优于盐酸哌甲酯缓释片。结论tDCS能提高ADHD患儿的自我意识水平,促进其自我控制的能力。其结果可能与tDCS机制有关。
