In the present study, the effect of blockage of the costimulatory signal CD86 at time of implantation on the expressions of TGF-β1, MMP-9, TIMP-3 and PAI-1 proteins at the maternal-fetal interface and the outcome of ...In the present study, the effect of blockage of the costimulatory signal CD86 at time of implantation on the expressions of TGF-β1, MMP-9, TIMP-3 and PAI-1 proteins at the maternal-fetal interface and the outcome of pregnancy in mufine abortion-prone model was investigated, in which the CBA/J × DBA/2 matings were used as the abortion-prone model and the CBA/J × BALB/c matings used as the normal pregnant model. The study was performed in following three groups： 2 groups of the abortion-prone model, which were experimental group and control experimental group, and 1 group of normal pregnant model, and each group had 10 pregnant CBA/J mice exclusively. Female pregnant CBA/J mice in the experimental group received an intraperitoneal （i. p. ） injection of 100μg of antimouse CD86 mAb in 200 μl of PBS at day 4.5 of gestation, and the irrelevant-isotope matched rat IgG2b was administrated in the control experimental group with the same dosage and at same time. For the normal pregnant group, no treatment was given. The pregnant CBA/J mice were killed on day 13.5 of gestation. Then, the embryo resorption rate was calculated and the expressions of TGF-β1, MMP-9, TIMP3 and PAI-1 were detected by using immunohistochemical methods. It was demonstrated that the embryo resorption rate in the experimental group was significantly reduced in comparison with that in the control experimental group （X^2 = 7.441, P = 0.006）, but there was no significant difference with that in normal pregnant group （X^2 = 0.016, P = 0.898）. The expressions of TGF-β1 and PAI-1 in the experimental group were significantly increased in comparison with that in the control experimental group （P =0.010, P = 0.003, respectively）, with no significant difference from that in the nonnal pregnant group （P = 0.500）. However, the expression of MMP-9 in the experimental group was significantly reduced in comparison with that in the control experimental group （P = 0.012） with no significant difference from that in the normal pregnant group （P = 0.500）. The expression of TIMP-3 in the experimental group showed no significant difference both with the control experimental group （ P = 0. 328） and the normal pregnant group （ P = 0. 500）. It is concluded that the blockage of the costimulatory molecule CD86 at early stage of gestation can render TGF-β1, MMP-9, TIMP-3 and PAI-1 proteins to express their immuno-tolerant effects through their characteristic pathways and induce the reduction of the embryo resorption rate in the natural abortion-prone model of mice to the level of normal pregnancy.展开更多
To investigate the expression of Bcl-2 and Survivin and HPV16/18 infection in uterine cervical carcinogenesis, formalin-fixed, paraffin-embedded specimens from cervical carcinomas, cervical intraepithelial neoplasia ...To investigate the expression of Bcl-2 and Survivin and HPV16/18 infection in uterine cervical carcinogenesis, formalin-fixed, paraffin-embedded specimens from cervical carcinomas, cervical intraepithelial neoplasia （C1N） and normal cervical tissues were studied. Using streptavidin-biotin peroxidase （S- P） immunohistochemical technique, the authors examined the expression of Bcl-2 and Survivin in these specimens. The number of apoptosis cells was assessed in situ by TdT-mediated dUTP-biotin end labeling （TUNEL） method. The infection of HPV type 16, 18 DNA were determined by PCR. It was found that there were significant differences in Bcl-2, Survivin and apoptotic index （AI） between cervical carcinomas, CIN and normal cervical tissues, respectively. Expression of Bcl-2 and AI were correlated with tu- mor grades, clinical stages and lymph node metastasis and expression of Survivin was associated with tu- mor grades and lymph node metastasis. There were different positive rate of HPV^s between cervical car- cinomas, C1N and normal tissues and were not associated with tumor grades, clinical stages and lymph node metastasis. The infection of HPV16/18 was associated with the expression of Bcl-2, Survivin and AI, respectively. It was concluded that the abnormal expression of Bcl-2, Survivin and infection of HPV16/18 were associated with cervical carcinomas. They possibly can be useful indexes for the primary screening and prognosis of cervical carcinomas.展开更多
文摘In the present study, the effect of blockage of the costimulatory signal CD86 at time of implantation on the expressions of TGF-β1, MMP-9, TIMP-3 and PAI-1 proteins at the maternal-fetal interface and the outcome of pregnancy in mufine abortion-prone model was investigated, in which the CBA/J × DBA/2 matings were used as the abortion-prone model and the CBA/J × BALB/c matings used as the normal pregnant model. The study was performed in following three groups： 2 groups of the abortion-prone model, which were experimental group and control experimental group, and 1 group of normal pregnant model, and each group had 10 pregnant CBA/J mice exclusively. Female pregnant CBA/J mice in the experimental group received an intraperitoneal （i. p. ） injection of 100μg of antimouse CD86 mAb in 200 μl of PBS at day 4.5 of gestation, and the irrelevant-isotope matched rat IgG2b was administrated in the control experimental group with the same dosage and at same time. For the normal pregnant group, no treatment was given. The pregnant CBA/J mice were killed on day 13.5 of gestation. Then, the embryo resorption rate was calculated and the expressions of TGF-β1, MMP-9, TIMP3 and PAI-1 were detected by using immunohistochemical methods. It was demonstrated that the embryo resorption rate in the experimental group was significantly reduced in comparison with that in the control experimental group （X^2 = 7.441, P = 0.006）, but there was no significant difference with that in normal pregnant group （X^2 = 0.016, P = 0.898）. The expressions of TGF-β1 and PAI-1 in the experimental group were significantly increased in comparison with that in the control experimental group （P =0.010, P = 0.003, respectively）, with no significant difference from that in the nonnal pregnant group （P = 0.500）. However, the expression of MMP-9 in the experimental group was significantly reduced in comparison with that in the control experimental group （P = 0.012） with no significant difference from that in the normal pregnant group （P = 0.500）. The expression of TIMP-3 in the experimental group showed no significant difference both with the control experimental group （ P = 0. 328） and the normal pregnant group （ P = 0. 500）. It is concluded that the blockage of the costimulatory molecule CD86 at early stage of gestation can render TGF-β1, MMP-9, TIMP-3 and PAI-1 proteins to express their immuno-tolerant effects through their characteristic pathways and induce the reduction of the embryo resorption rate in the natural abortion-prone model of mice to the level of normal pregnancy.
文摘To investigate the expression of Bcl-2 and Survivin and HPV16/18 infection in uterine cervical carcinogenesis, formalin-fixed, paraffin-embedded specimens from cervical carcinomas, cervical intraepithelial neoplasia （C1N） and normal cervical tissues were studied. Using streptavidin-biotin peroxidase （S- P） immunohistochemical technique, the authors examined the expression of Bcl-2 and Survivin in these specimens. The number of apoptosis cells was assessed in situ by TdT-mediated dUTP-biotin end labeling （TUNEL） method. The infection of HPV type 16, 18 DNA were determined by PCR. It was found that there were significant differences in Bcl-2, Survivin and apoptotic index （AI） between cervical carcinomas, CIN and normal cervical tissues, respectively. Expression of Bcl-2 and AI were correlated with tu- mor grades, clinical stages and lymph node metastasis and expression of Survivin was associated with tu- mor grades and lymph node metastasis. There were different positive rate of HPV^s between cervical car- cinomas, C1N and normal tissues and were not associated with tumor grades, clinical stages and lymph node metastasis. The infection of HPV16/18 was associated with the expression of Bcl-2, Survivin and AI, respectively. It was concluded that the abnormal expression of Bcl-2, Survivin and infection of HPV16/18 were associated with cervical carcinomas. They possibly can be useful indexes for the primary screening and prognosis of cervical carcinomas.
